LOCUS       NZ_OY754391             5429 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F637 plasmid pF637-2, complete sequence.
ACCESSION   NZ_OY754391
VERSION     NZ_OY754391.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542884
            Assembly: GCF_963575425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:17:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,851
            CDSs (total)                      :: 4,736
            Genes (coding)                    :: 4,461
            CDSs (with protein)               :: 4,461
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 275
            CDSs (without protein)            :: 275
            Pseudo Genes (ambiguous residues) :: 0 of 275
            Pseudo Genes (frameshifted)       :: 75 of 275
            Pseudo Genes (incomplete)         :: 190 of 275
            Pseudo Genes (internal stop)      :: 47 of 275
            Pseudo Genes (multiple problems)  :: 40 of 275
            Pseudo Genes (short protein)      :: 4 of 275
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754391.1.
            Complete genomic sequence of E. coli strain F637.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F637"
                     /db_xref="taxon:562"
                     /plasmid="pF637-2"
     gene            748..1158
                     /locus_tag="R8926_RS24220"
                     /old_locus_tag="FGAF637_46990"
     CDS             748..1158
                     /locus_tag="R8926_RS24220"
                     /old_locus_tag="FGAF637_46990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874179.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     gene            1416..1979
                     /locus_tag="R8926_RS24225"
                     /old_locus_tag="FGAF637_47010"
     CDS             1416..1979
                     /locus_tag="R8926_RS24225"
                     /old_locus_tag="FGAF637_47010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001749520.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(2008..2634)
                     /locus_tag="R8926_RS24230"
                     /old_locus_tag="FGAF637_47020"
     CDS             complement(2008..2634)
                     /locus_tag="R8926_RS24230"
                     /old_locus_tag="FGAF637_47020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223149424.1"
                     /translation="MSPTLNKQASKERCAVEFATRKKSKTQRKKSKALSYRGLCFFCTL
                     VTGKCQWTSKMSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDLNGLV
                     FGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHINDNK
                     EKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            complement(2936..3019)
                     /locus_tag="R8926_RS24235"
     CDS             complement(2936..3019)
                     /locus_tag="R8926_RS24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532987.1"
                     /translation="MHEPPVQSDRCAFSGNYRLESNPERHD"
                     /name="replication initiation protein"
     gene            3657..3977
                     /locus_tag="R8926_RS24240"
                     /old_locus_tag="FGAF637_47030"
     CDS             3657..3977
                     /locus_tag="R8926_RS24240"
                     /old_locus_tag="FGAF637_47030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957082.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4186..>4620
                     /locus_tag="R8926_RS24245"
                     /pseudo=""
     CDS             4186..>4620
                     /locus_tag="R8926_RS24245"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4655..5140
                     /locus_tag="R8926_RS24250"
     CDS             4655..5140
                     /locus_tag="R8926_RS24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749519.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            5144..5356
                     /locus_tag="R8926_RS24255"
     CDS             5144..5356
                     /locus_tag="R8926_RS24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3370..3549
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          complement(2911..3424)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.217"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3968..5427
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.84"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 accccgtttc cgtcagcgga gctataacgg tccgacgctg ggctgactgc gcgcatccag
       61 tgacaaaccc caagaagaag ggctcataaa tttcctgcta aatacggtcg tgatgaaaag
      121 atcacggctc aagagagggg gatttatgaa gatactaaaa gcgctcagtc tgatactgca
      181 atgcgtcaaa ctggcaatgg atctgtggga gcgattcggg ggaaagtggc ctttttagtg
      241 ctttacaccg tatttatacg cgaaaactgc tgtcattcat ctggctgcat aaccatgcag
      301 ccaagtgaaa tttatctggc gtaaacgggg cggtttaccg ggtgctttgc taggggtttc
      361 acccgacagg caccgtattt gcgccgaacc agcccgaagc ggtgcgggca agaactctta
      421 tgttaaattc atctcatcgg caacctatac aggcataaaa atcagtttct ataagcggct
      481 ttcttgcacc tacttggtga ttttttggat gcgctgacgt tgctcgactt taaatcacca
      541 tagaaaaata ccctttactt acataaagat cgatctggat gtgtatcttg acgaggttca
      601 atacgagcaa ttgtttttgt atttaaggtt tcttatcgcc gttttttgcg gctctagaaa
      661 aatcaataca aaagatcttg aaagccacct cgctccctgg gtacacttcc gtataagaac
      721 tgatcgatgt ctaaaacgga ttgtattatg gcttttatct ctctatcgaa cttggcagcg
      781 aagactggaa ggataatcca gtcgcagtgt gttacggcca cgcaaacgaa aaaaagcacg
      841 ggcgctgtcg agctgggatt ccgcttgagc aagagcgcta tggacgagac tggtattacc
      901 attggggatc gtgtcgacat tctttacgac aaagaagaaa accagtggat gattaagaaa
      961 attagtgaag gtggctttgg tgtgtctggg caaaaaaaca agcacggcgg ttactcatcg
     1021 tgtgctgtta gattgacctt gcgaccaggt tttcctcgcc tttctgagga tgaagaggac
     1081 aacaagaagc tactttccga tgattcattc actcagctaa gtgaaggtgt aatcgtcttc
     1141 aggctggatg atgaatagga aggaccaaaa aggaaaggca gagttagtga gcgtcgccaa
     1201 acaatcgact aactccgcct tcttgtcaac acatcactac ctttggaagg gtggagtgtt
     1261 atgggcggca ttatgttcat aaacttgcat tctaataaag aaaaaatcgt ttcttttgag
     1321 ggatgtgaac acgacatttt ctattctgat aggtgcgatg ctattggctt tggtctctta
     1381 agtttatgtt tttaaaaaca tatgaggcta tacatatgca cgctttatca atactacctt
     1441 cagttgcccg cgctaacctg gcaactaaat tctcgtctca tttgaaggtg atcgagctct
     1501 tcaacacgat gcaggattcc caagttgttg tcttgtcatc actgatcgaa ggccatcacc
     1561 tcaccagttc tggtaacagt gtcaaagccg actttgaggt cactcgctta ccggcgatta
     1621 tcgagatgtt agagaaaaaa tattttttcc ctattcgtca tctaaatgta tctgttaaat
     1681 ctgtaactac aggaaggatg actggacaaa ctgtctattt catagagcct gaacacatcg
     1741 agcaacttct cgctgatcct gagcttgtgt ttgctaatca ggaaaggagc ctcttctttc
     1801 gttctctcga aaaagaaggg aaaaatctcg gcaagcttat cgagaaaaaa ggtagtgttt
     1861 ctcaagccgt cttaagcctt ctacatcacg cctacaaaga taaacctctt tctgacgaga
     1921 tgtggaagga gatagaagat aagttcaccc atatgctgga tgaattgagc gcggcgtaaa
     1981 ggcaagggcg gtcgttagac cgccttttta gtcttctaaa cctaattctt tgtgatagtt
     2041 tttcagcgta gagtaacgat agtttctgag tatgttttca tctttcttga cgttcgcttc
     2101 tatgtatctg tttacgaatt tgttgaaatc ttcttgtatt ttttctttgt tatcattgat
     2161 atgggtaaat tcaggagttg gtatcttata ggctcgcgat acatactctt ctttcttaat
     2221 tagcaacgct ttggtgtaat ccagtccttt cttaacacct tctgatcgct ccgaaacgaa
     2281 tccaaattta tggtttaaat ggcttcgtaa gggtatcccg aacaccaatc cgttgagatc
     2341 aataactacg attccgtagc ctcttacttt tccatcatta ctgccgtcga gagcctcttg
     2401 taggcctttg ttatcagcat aaaactggtc tgtaagggtt cttaactgta tgtaatcctg
     2461 tttgctcatt tttgatgtcc attggcattt tcctgtaacc aatgtacaaa aaaagcaaag
     2521 ccctctgtaa gagagggctt tgcttttctt tcgttgagtc ttggattttt tacgtgtggc
     2581 gaactctacc gcacatcttt ctttggaagc ttgtttgttt aacgtggggc tcattcctac
     2641 cgccacttct agatttactt atatcaaaaa ttatatgtga agtcaatttc gtagctgttc
     2701 ttgactggac ttactttgcc agcgtaatct tctcctgaaa cgaaaaaacc accctggcag
     2761 gtggtttttc gaaggttatt taatcctggc agattatcta accgtggtaa cagtcttgtg
     2821 cgagacgtgt caccaaatcc gtccttccag tgtagcctca gtaaggccac cacttcaaga
     2881 actctcaata catctctcgc acatcatgtt taccagtggc cgttgccaat ggcgattagt
     2941 cgtgtctttc cgggttggac tcaagtcgat agttaccgga aaaggcgcag cggtcggact
     3001 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
     3061 tcaggcgtgg aatgagataa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
     3121 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttatagt
     3181 cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     3241 cggagcctat ggaaaaacgg cagcgccgca tccttttctt gccctgtttg tcatgccgta
     3301 tgcctttgct cttatccgtg tgcaggtaac gcccgccgca gccgaacggc aggagcggaa
     3361 gcgaccgagt gagcgaggag gcgtaatatc accgcaacct ccatgttctc ccgtacgccc
     3421 tgcagctgca ttttcccctg ccacatgaag cacttttata aagtgttact ccgccgtaac
     3481 atattaagcc agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa
     3541 acccgctaaa agccactgac gcgcctgcgg cttgtccaac accgtgccga ccgggaaaca
     3601 tttcccgccc gtcccggtgt tatcaggagg ctactctggc agacaaacgc aacaagatgc
     3661 tcacgatgtg ggtgacggaa gacgaacacc ggcggctgct cgagcggtgc gacggcaagc
     3721 agctggcggc gtggatgcgt cagacctgcc tcgatgagaa gccggcgcgt gccggcagac
     3781 tgccgtcgat atcgccggcg ctgctgcgcc agcttgccgg tatgggtaat aacctgaacc
     3841 agatcgcccg gaaagtgaat accggtggtg ccggtcatga ccgggtgcag atcgtggccg
     3901 ccctgatggc aatcgacgcg ggactggagc ggctgcggca tgcggtgctg gaaaaagggg
     3961 ggaatgatga tcgttaaatt ccatccccgg gggcgcggtg gtggtgccgg tccggtggat
     4021 tacctgctgg gaaaagaccg gcagcgcgag ggggcgaccg tgctgcaggg aaagccggag
     4081 gaagtccggg agcttatcga tgcctcgccg tatgcgaaaa agtacacctc cggcgtactg
     4141 tcgttcgcgg aggctgagct gccgcccggg cagcgggagc agatcatggc cagctttgag
     4201 cgggttctga tgcccgggct cgataaagac cagtacagca ttctgtgggt ggagcacacc
     4261 gacaaggggc ggctggagct gaatttcctg atccccaaca cagagctgct gaccgggaaa
     4321 cgcctgcagc cgtattacga tcgcgccgac cggccgcgca tcgatgcctg gcagaccgtg
     4381 gtgaacggca gactggggct gcatgatcca aacgccccga agaaccggcg gttactggtc
     4441 acgccgtccg cactgccgga aacgaagctg gaagccgctc aggcgattac acggggtttg
     4501 ctggccttag cctcatccgg ggcgctcaaa acgcgcgagg acgtcacagg gtcgctcact
     4561 gcggcaggtt ttgaggtggt ccgcaccaca aagaacagta tcagcattgc cgacccggac
     4621 ggggggcgaa acatccgact gaagggagcc atttatgagc agtcttttag cgctggcgaa
     4681 ggacttggaa aagcaatcga aagcgcagca gcagaatacc tgcgagatgc tgaaagccgc
     4741 attcagcgag cacgagaagt ctgtaaaagc ggaactgagc gcaagcgcga agagaatcag
     4801 cgacgccatc agcgcccacg agcagggtat gaccgcggcg atgcagtcga accggctgag
     4861 cgtgctgcgt atggttggcc ggacgtggct gaccatcacc ctggtctcag tactgctgat
     4921 cgccacgagc gggagcattc tgtggtggca ggggcagcag atcaccggga attaccagac
     4981 tatccgggcg caggagcgga cgcaggccat gctgtcggag aagaaccacg gcgtgcagct
     5041 gtcgctctgt ggggaacaga aactgtcctg cgtcaaagtg aacccgaaag cgggagctta
     5101 cggggaggaa gggaactgga tggtactgga gaggaaatag cccatgacgg aactggaaaa
     5161 acagttgctg accgcattag agcagctaca tcaggactac tcgcaaaggc tggacgagtg
     5221 ggagagcgcc ttcgcggaat ggcagaagat gtctggtctt atgcaacggg agaacgcggc
     5281 gctgaacgag cgcgtcacgc gcttgagtca gcaggtggcg aacttgagca ggcagttaca
     5341 gcgtttgagc cagtagtgca gcggcatgaa atggcggtgg ccgccgaacg ggcatacgag
     5401 cagcgacagc atgaaaaaga gctggcaga
//
