LOCUS       NZ_OY754387             4018 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F138 plasmid pF138-5, complete sequence.
ACCESSION   NZ_OY754387
VERSION     NZ_OY754387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542932
            Assembly: GCF_963575405.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:22:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,099
            CDSs (total)                      :: 4,981
            Genes (coding)                    :: 4,739
            CDSs (with protein)               :: 4,739
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 72 of 242
            Pseudo Genes (incomplete)         :: 158 of 242
            Pseudo Genes (internal stop)      :: 47 of 242
            Pseudo Genes (multiple problems)  :: 34 of 242
            Pseudo Genes (short protein)      :: 3 of 242
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754387.1.
            Complete genomic sequence of E. coli strain F138.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F138"
                     /db_xref="taxon:562"
                     /plasmid="pF138-5"
     gene            complement(<344..1048)
                     /locus_tag="R8951_RS25465"
                     /old_locus_tag="FGAF138_49170"
                     /pseudo=""
     CDS             complement(<344..1048)
                     /locus_tag="R8951_RS25465"
                     /old_locus_tag="FGAF138_49170"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(987..1310)
                     /locus_tag="R8951_RS25470"
                     /old_locus_tag="FGAF138_49180"
     CDS             complement(987..1310)
                     /locus_tag="R8951_RS25470"
                     /old_locus_tag="FGAF138_49180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1958..2071
                     /locus_tag="R8951_RS25475"
     CDS             1958..2071
                     /locus_tag="R8951_RS25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2500..2712)
                     /locus_tag="R8951_RS25480"
                     /old_locus_tag="FGAF138_49190"
     CDS             complement(2500..2712)
                     /locus_tag="R8951_RS25480"
                     /old_locus_tag="FGAF138_49190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(2967..3494)
                     /locus_tag="R8951_RS25485"
                     /old_locus_tag="FGAF138_49200"
     CDS             complement(2967..3494)
                     /locus_tag="R8951_RS25485"
                     /old_locus_tag="FGAF138_49200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(3623..3838)
                     /locus_tag="R8951_RS25490"
     CDS             complement(3623..3838)
                     /locus_tag="R8951_RS25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1424..1601)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          1548..2071
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.434"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..997)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.783"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(3532..4016)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.654"
                     /coverage="99.0"
                     /evalue="1.9099999999999999e-81"
ORIGIN
        1 tatctgttgc ccctgccacc acagaatgcc cgcgctcgag gcgatcagta gcgcggagac
       61 caggactatg gtcagccacg tctggctgat catacgcagc atccctttcg tgcgttggct
      121 catggctgag gacagcttcc ggtcgtggtc gaggatggcg gcgctgattc tcttctcgct
      181 ttcgctcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt tcagcatctc
      241 gccggtagtc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca gcgttaaaag
      301 actgttcata gatggctccc ttcagtcgga tgtttcgccc cccgtcaggg tcggcaatgc
      361 tgatgctgct tttcgtggtg cgcaccacct caaaacccgc actttccagc gcctcagtga
      421 cgtcctgacg ggttttcagc tccccggatg aggcaaggga aagtaggccg cgcgtaatcg
      481 cctgggcggc ttcctgcttc gcttccggca gcgcggacgg tgtaaccagc gcccgccggt
      541 tttccggcgc gttcgggtcg tgcagcccca gtctgccgtt cactatggtc tgccaggcat
      601 caatgcgcgg acggtcggcg cggtcgtaat acggctggag acgtctgccg gtcagcagct
      661 ccgtgttcgg gatcaggaag ttcagctcca gccgcccttt gtcccggtgc tcaacccaca
      721 gtacgctgta ctggtcttta tcgagtccgg gcatcagaac ccgctcaaag ctcgccatca
      781 gcttttcacg ctggccgggc ggcaaatcct gttcggcaaa agacagcacg ccggaggtgt
      841 atttcttggc gtaaggcgag gcgtcaataa gctcccgcac ttcctccggc ttaccctgca
      901 gaacgctggc gccgtcgcgc tgccggtctt tccccagcag ataatccacc ggaccgccgc
      961 caccgccgcg gcctctggga tgaaacttaa cgatcatcgt ctgccccctt ttccagcacg
     1021 gcgtgccgca gccgctcgag tccggcatca atggccatca gcacggcgac aacctgcacg
     1081 cgatcgtgcc cagtaccgcc accggcgtta acctgccggg caatctggtt gaggttgtta
     1141 cccatgccgg caagctgacg aagcagcgcc ggcgagatgg atggaagttt gccagcacgc
     1201 gagggctttt cgtccaggca tatctggcgc atccaggccg cgagttgttt accctcacag
     1261 cgctccagaa gtcggcggtg ctcgtcttct gtcacccaca tagtgagcat tttgttgcgc
     1321 ttttctgcca aaccatcctc cggataacac cggttcgggc gggaaatttt ttcctgtccg
     1381 gagcggtgtt gggcaagccc gcagggcgcg gcagtacttt tcagcgggtt tcggggcgca
     1441 gccctgaacc agtcacgtag cgctagcgga gtgtatactg gcttaacatg gtaaatcgga
     1501 gtggggattc aggaaagtgc ttcatgtggc agggagaaaa tgctgctgtg gggcgtaagt
     1561 gcgaaatgca gatcccggtg aaatgacgcc tcctcgctca ctcggtcgct gcgctcctgc
     1621 cgtgagactg cggcgggcgt tgccagctta cgaatggctg gataaagaga ctaagcggaa
     1681 atcaatcaga aagggcggaa aaaggatgcg gcgttgccgt ttttccatag gctccgcccc
     1741 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     1801 taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt tcctgccttt
     1861 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat ctaattccac gcctgacact
     1921 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     1981 cgaccgctgc gccttatccg gtaactatcg acttgagtcc aacccggaaa gacacgacaa
     2041 aacgccactg gcagcagcca ctggtaactg atttagagga gttagtcttg aagttatgcg
     2101 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg tgctcctcca agccagttac
     2161 ctcggttcaa agagttgctg ctctgagaac cttcgaaaaa accgccttgc tgggcggttt
     2221 tttcgtttac agtgctaaga gattacgatt ctccatgaat gatctcaaga agatccgttt
     2281 aaaaaagcgg gttttatggg gggataaagt tcgtctagcc cagtcacttt ggattacagc
     2341 taggtttcgt aagccatgca agaatgcctg taactaacaa aataggccct gcaacgtacg
     2401 taacatagac tccaacatac gacactgtga aacatatgag tacgtatata gacagtgttg
     2461 tccaaaaaat gactgttgtt ttacagtgaa tccactcttt catttttcaa taaaccccac
     2521 accgtctgga gatcttatta tcgttttgcc aggattagct ttcgcccaaa ccaaagcatc
     2581 ttgaaaagaa gagaattttt tttttgaatt tttagttttt cggcgatctt ctcgaaaata
     2641 ttcgctgtta ttctcatcaa ttatccgatc aaaatcagca ccagctccaa aggtacgctc
     2701 aaaatccccc attttctgcc cccattaaat ttaataaaaa aacagattta aaatatccaa
     2761 aagacactct tatttaccat aacatccctt gttcgcaccg ccgcagacag cctcagcacc
     2821 gttctgcgcc atgaaccgca aaagagcacc tcttaccacg taaaaacgcc gataaatgcc
     2881 ccccgtaagt tcttacggta tgcatagtta tgcacggaaa tgaactgaca ctctgttcaa
     2941 tggattttca tgctgtaagc agtattttag tcttctatgc cccctcgaaa cacacctgtt
     3001 cttgtgtaga tcttttcgac ccaagatggt cttttatccg agttcatgca gccgtacagg
     3061 aacacacctg ttttgggttt atagctcccc ccctcctgtg gctggttatc tacaaatgaa
     3121 taatcaatct tttcaccttt gactgaagca cgccccccaa catgaactaa agaccagtgc
     3181 tcatgcatca catggctttc aatcaatcca aatgaagaaa aaccccatga atcaaaaaag
     3241 accttgagtc cgtcagacgc accaacagga gtaatgattg caaattcaac ctgctcacca
     3301 gtaccttcaa ggtaagaagg tttctcgacg ttaattacag atgccccctt cacctgataa
     3361 cctttacagg taagatataa gtccctcccg gctgacggtt ttgtatatgt aacgttgtcg
     3421 ttattctccg cagcagcgac tgtaaatgac atagccaaaa ccaccgagac tgagcaagcc
     3481 cagaaacatt tcataacgca tcccttttca ttgattaact acattccaag ctcaaaacct
     3541 ttctgccgct gcgatcgttc cagctccagc gctttctggt gctgcgccgc gcgctcctgc
     3601 tcccggtgcg cctcgatgcc attcagcctt gcgacaatcg gcgcagctga tcgctcaaac
     3661 tctgcacctg cctgctcaag cccgtgacgc gctcgctcag cgccgcgttc tcccgttgca
     3721 taagcccaga catcgtccgc cattccgcga aggcgttctc ccactcgtcc agccttttcg
     3781 agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg tttttccagt tccgtcatgt
     3841 gctatttccc cgccagtatc atccagctcg aatcctctcc gaatcttccc gcttccgggt
     3901 tcaccctcac gcagcggcgt ctctcctccc cgcaggtcgt gagctggacg ccgttgttct
     3961 tctctgaaag catggcctgc gtgctcttct gttcctggat ggtcgtgtag ttgtcgag
//
