LOCUS       NZ_OY754376             2442 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F374 plasmid pF374-5, complete sequence.
ACCESSION   NZ_OY754376
VERSION     NZ_OY754376.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542958
            Assembly: GCF_963575375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:03:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,624
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,266
            CDSs (with protein)               :: 5,266
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 83 of 235
            Pseudo Genes (incomplete)         :: 162 of 235
            Pseudo Genes (internal stop)      :: 50 of 235
            Pseudo Genes (multiple problems)  :: 53 of 235
            Pseudo Genes (short protein)      :: 5 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754376.1.
            Complete genomic sequence of E. coli strain F374.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F374"
                     /db_xref="taxon:562"
                     /plasmid="pF374-5"
     gene            199..681
                     /locus_tag="R8837_RS28110"
                     /old_locus_tag="FGAF374_53830"
     CDS             199..681
                     /locus_tag="R8837_RS28110"
                     /old_locus_tag="FGAF374_53830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     gene            668..898
                     /locus_tag="R8837_RS28115"
                     /old_locus_tag="FGAF374_53840"
     CDS             668..898
                     /locus_tag="R8837_RS28115"
                     /old_locus_tag="FGAF374_53840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(968..1936)
                     /gene="mobV"
                     /locus_tag="R8837_RS28120"
                     /old_locus_tag="FGAF374_53850"
     CDS             complement(968..1936)
                     /gene="mobV"
                     /locus_tag="R8837_RS28120"
                     /old_locus_tag="FGAF374_53850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     Typing          complement(972..1945)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 cttgtaccaa gggctagagc gaagaatcgt actcacctca agtactaatc gtactcacct
       61 caagtactaa tcgtactcac ctcaaggact aatcgtactc acctcaagta ctaatcgtac
      121 ttgatacaaa ggacttgact gtatgtctct ttagtactta aatgcgagta ctaaatcatg
      181 gcgaaacgtg gggctggagt gaatacgaaa aagatttcta ggcttgaacg gcatacatac
      241 atggatgaaa acggtgaggt aaaacaagac tctaagatcg agaccttttc catcccagcc
      301 gaacctccct acgtcaagct atacattgca gacattgcga tgattcatgg tttatcaaaa
      361 tctcaaaatg atgtgctttt acaattggca atgctgattg gatgggatgg gtttgtgtct
      421 gtatcaaaaa ctagatttga aaaaaccata cagcccaggg ttcatattga atatcagacc
      481 tttaaaaaca taattgcggc tcttgtagat aaacaaatat tcttccgggc agggcgtggg
      541 gaacttgaag ctaacccctt tctttttgca cggggagact ggccatcaat ttacgaaaga
      601 agaaagaaaa ttgagttaaa gatcagttat gacaccaatg gaagaacggt gcagacgaag
      661 gtaatggatg atgaatgctg aagataaact ttttaaaaat gtagacaggc taatgctgca
      721 tgattcacta aaaaaaaatg aggttctgac tgtttgggag cgtacttttt taagcagtgt
      781 ttacagcata tatcgtagaa ctagcggtaa taaatactca actaagggtt tgtcaccaaa
      841 gcagaaaaac gcagcttttt ctattttaaa aaaacataat taccatctga atgattaatt
      901 ttttcgtggt gaatgccgca aatgacgtca ctttgcggcg ttagccctat cctgcatcgc
      961 atcaaaacta acgctccata ccacccatgc cacgcgatcg cgtctgtcgc tgtttttcac
     1021 gttcaatacg ctgctgtttt tccttttcct ggttttgctg cttaaaccgc tgagccatca
     1081 tcaggatttc ggtcatctgc tctctggaaa gccccttaaa aggctctgtg acgctctgta
     1141 ggcgtttctg ctgcgcatcc atagtcttct gtagctcacg cgctcttttt gcgttctggg
     1201 cgctctgaga ggccatagcg atggttcctg caaagccatc gttcactgcg tggcttagac
     1261 gtttggctat ggctgcctga tcttccacgt ctttggtgaa aatgcccttc cgcaatacac
     1321 gcggctccag cgcctccggc gagatcgata cctggctacg ggtgccacgg tttatcgatt
     1381 cgtagtagtg ctgaactgtc tgatgcgtgg cgcggcttcc ctcgatgccg cgctcaagac
     1441 ccagtttttt tactgattca gcgtaggtgc tctgatcttc acgcatcttt gaccgcccgc
     1501 caataaactc tttcgcggac agccgtccat cctgcgtcaa tggcaccacg aacgcagaca
     1561 ggtgcggggt ggcctcgtcc cggtgaacca ccgccgccac aacacgatcc ttcccgtatt
     1621 tcttctccag ccattgctca gagcgggcga aaaactccgc ctgctgccgt ggtgtggcct
     1681 ctttccacca ctcagggctt gctgtcataa cgtattcaac ggccagtacc gcatctttac
     1741 ggcgtttttc cggcagcagc tcccgcacac gccccattgc ctgatccgtt gatttggaca
     1801 cagagcagta gttttctggt gtccgttcag cgttagcgtt cggcgtctca cgctctcggt
     1861 agcagtgctg caacgcgctg gccacgcttc ccattcccgt cagctttttg cagcgcatta
     1921 tcgcgtagtt cgccatgttc ccccctgatt ccggcattca ttcagccagg cgggggagcg
     1981 cattgcgata cctccggcgg gccactatca agatgatagt gtactcacta gactttgcta
     2041 cgcaaaatcg ttcgggcagc aagctgccag aaggtcaaaa tcaacggcgg tctggcaggt
     2101 ggaaggcgtc gcttcgcgcc accccccacc actgccaagc ctgttttatc cggagtgtcg
     2161 tcaagggtaa atggcactcg ctacgctcgc ccttgacgaa atcaggcaat gaaatcgtct
     2221 ttgaaagagc aggaaccgct cgccgcagct ttggctgagg cgtaccgcag gagcaacgcg
     2281 acgaggaacg aacagccaaa gcgaggaagc ggaatttcca ctccccgtac atagaaatgc
     2341 gtacactcct tgctaaaaaa aggcagtttt tcagtgtcta aaaatgccgt ttttcagccc
     2401 ttattttccc ccttaatata tagatataga tatgggaacc cg
//
