LOCUS       NZ_OY754375             3082 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F374 plasmid pF374-4, complete sequence.
ACCESSION   NZ_OY754375
VERSION     NZ_OY754375.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542958
            Assembly: GCF_963575375.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:03:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,624
            CDSs (total)                      :: 5,501
            Genes (coding)                    :: 5,266
            CDSs (with protein)               :: 5,266
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 83 of 235
            Pseudo Genes (incomplete)         :: 162 of 235
            Pseudo Genes (internal stop)      :: 50 of 235
            Pseudo Genes (multiple problems)  :: 53 of 235
            Pseudo Genes (short protein)      :: 5 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754375.1.
            Complete genomic sequence of E. coli strain F374.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F374"
                     /db_xref="taxon:562"
                     /plasmid="pF374-4"
     gene            complement(join(2709..3082,1..349))
                     /locus_tag="R8837_RS28085"
     CDS             complement(join(2709..3082,1..349))
                     /locus_tag="R8837_RS28085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139821448.1"
                     /translation="MALMVTRRYLLGGEYPSEEFVQASLEKYFFRQGFDIDTDSYIDLI
                     CRDKESRDIVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIE
                     KTSILAVSKLNLHWIFVEPDGSVTIKKPDSSMMKNGNGNGREVSLVNGVFKRFIPGDFM
                     PTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPNIADIKAEAESRSGKWTLSNVKIEY
                     QFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(421..720)
                     /locus_tag="R8837_RS28090"
                     /old_locus_tag="FGAF374_53790"
     CDS             complement(421..720)
                     /locus_tag="R8837_RS28090"
                     /old_locus_tag="FGAF374_53790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139821447.1"
                     /translation="MANITNFDMWLDDVDGDHEEVMALYDAVQNVSDMGVYKCVEGNGR
                     DTWVVSSNHHHEDLFLESVVDKGDFLKLIKKRLCGGENVESWYGFQRNMSNDHS"
                     /name="hypothetical protein"
     gene            complement(1057..1380)
                     /gene="mobA"
                     /locus_tag="R8837_RS28095"
                     /old_locus_tag="FGAF374_53800"
     CDS             complement(1057..1380)
                     /gene="mobA"
                     /locus_tag="R8837_RS28095"
                     /old_locus_tag="FGAF374_53800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1665..1859
                     /locus_tag="R8837_RS28100"
                     /pseudo=""
     CDS             1665..1859
                     /locus_tag="R8837_RS28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2355..>2435
                     /locus_tag="R8837_RS28105"
                     /pseudo=""
     CDS             2355..>2435
                     /locus_tag="R8837_RS28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(1392..1627)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.479"
                     /coverage="98.0"
                     /evalue="1.3000000000000001e-110"
     Typing          1903..2427
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.774"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1061..1380)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.18e-62"
ORIGIN
        1 gtaaattcaa tttactaaca gcaagaatgc tcgttttttc tattaatctt tcatactgtt
       61 ttattcttgg aacagcaata gcataattaa tattatcttt tgtcattctc tgcactaatt
      121 gtccaagaca agttcgaaaa tccaaccctg gttgtgatgt tttccctttt acttcaatat
      181 gccacacaat atccctagat tctttatcac ggcaaataag gtcaatataa gaatccgtat
      241 ctatatcaaa cccttgacga aaaaaatatt tttctaaaga agcttgaaca aattcctcgg
      301 agggatattc accacctagt aagtaccttc tcgtcaccat aagagccaca aacccctacg
      361 catcctattt actataacgt catattttaa gagttcccac aaggagaact ctctgtacga
      421 ttacgagtga tcattcgaca tatttcgttg aaatccatac catgattcaa cattttctcc
      481 gccacatagt ctttttttaa tcaacttgag gaaatccccc ttatccacaa ctgattctag
      541 aaaaagatct tcgtgatgat gattcgagct cacgacccaa gtatccctcc cattaccttc
      601 aacgcactta tagactccca tatcacttac attctgaaca gcatcataaa gagccataac
      661 ctcctcatga tcgccatcaa catcatctaa ccacatatca aaattagtaa tattcgccat
      721 actatccccc cccccatttt tttagttttc agaagttaat ttacacagtg ataggggctg
      781 ttaagcaaat aagtttttac cttttttgcc tcatgctcta aaagtgagca aggtaggtat
      841 ttaccataac gtcgcattac gcgcaccgct aaaacgcgct gaacgcgatt ctgccgcaaa
      901 caccgcaaaa agagatgttt tccccggggt aaaccggaaa aatgcgtcag gatcgctttc
      961 aactcactgc atagctatgc atgaaagtga atggcgatcg gtttgggggc ttacggcgtt
     1021 cataccgtct gttttcgaca gtttctctcc gggaagctaa tctgccataa gcctggataa
     1081 cagggcccgg tgatactccg taatagcgat cagcacctcc gcatactccc tgtccccgac
     1141 acgcttgccg tcgataaaga gttttttctg caacgccccc agtcgcataa cttctttcag
     1201 cacccggtca tcagtcagtg agttaacttt cttaccgaga gctgccgccc gtaaaaaacc
     1261 ggaaacggtc ttgccgcatt cagcagccct ttttctgatt tcctggtctt cttctgccgt
     1321 cagacgaaca gggcgactga tagcccgcct gcgcgtatta cttccacttc gttttgtcat
     1381 aaaacatggt tccgtatctg acatggggtg tcggggcgaa gccctgacca gggtaattgt
     1441 aatagcgcgc atgtatgcgc ggtataacaa ttacacatcc tgtcctgtca gcaagttcga
     1501 attaccgata aaggcacggc agttttttag cagtgacagt attgtcaggc agaaatgccc
     1561 gtcaaagagt cagacggcag aataccggcg gtaaaacagt cgaaaagtgg tacgcgatat
     1621 aatcacacaa cgcagatgac agacagaacg gagaaaacga aaagatgaac aagcagcagc
     1681 aagccgtact caacatggca ggattcataa aaagccagag cctgacactg ctcgaaaagc
     1741 tggacgcact cgatgccgac gaataggccg ccatgtgtga gaagctgcac gaactcgcgg
     1801 aagaactcca gaacagcata cagacacgct ttgaagcgga aaatggcacc ggggtataac
     1861 agtatatccg gcaccagaca ggatgcagaa tggtatacct gtgcgtaagg agaaaatgct
     1921 acatcaggca cttttccgct tcctcgctca ctgactcgct gcgctcggcc gttcggctgc
     1981 ggcgagcggt atcagcccac tcaaagacgg taatgcggtg atccacagaa tcaggggata
     2041 acgccaaaag aaacatgtga gcaaagaaca ataacccgga aaaggccgcg ccagtggcgt
     2101 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2161 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2221 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2281 gcgtggcgct ttctcatagc tcacgctgtt ggtatctcag ttcagtgtag gtcgttcgct
     2341 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2401 actatcgtct tgagtccaac ccggtaagac acgactaatc gccattgaca acggccactg
     2461 gtagacagga tgtgcgagag atgtatcgag agttcttgaa gtggtggcct gactgcggct
     2521 acactgaaag gacagatttg gtgacatgtc tcgcacaaga ctgttaccac ggttcagaag
     2581 ttccccaact tactgaacct tcgaaaaacc acctccccag gtggtttttt cgttttccga
     2641 gaaagagatt acgctttgag cgaatgaatc tcaagaagat catggcatgt gatgaacaaa
     2701 ggagtcagct actcagattg atagaaaaaa tgccccttat ataagtgata tattttccaa
     2761 aactgatact ctattttaac attagaaagc gtccatttcc cactccgcga ttcagcctct
     2821 gctttaatat cagcaatatt tgggatcacc ccatttgaaa taacaaaatc gtccagccaa
     2881 tcccacattt ctcgccgaga tgatttaaat acaacaggag cttttccata tgagcaagtt
     2941 ggcataaaat cacccggaat aaatctttta aacaccccat tcacaagaga aacttctctt
     3001 ccattaccat tcccattttt catcatagag ctatcaggct tcttaattgt cacactgcca
     3061 tcaggctcaa caaatatcca gt
//
