LOCUS       NZ_OY754370             2182 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F404 plasmid pF404-6, complete sequence.
ACCESSION   NZ_OY754370
VERSION     NZ_OY754370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542961
            Assembly: GCF_963575385.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:01:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,048
            CDSs (total)                      :: 4,926
            Genes (coding)                    :: 4,730
            CDSs (with protein)               :: 4,730
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 79 of 196
            Pseudo Genes (incomplete)         :: 120 of 196
            Pseudo Genes (internal stop)      :: 36 of 196
            Pseudo Genes (multiple problems)  :: 39 of 196
            Pseudo Genes (short protein)      :: 3 of 196
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754370.1.
            Complete genomic sequence of E. coli strain F404.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F404"
                     /db_xref="taxon:562"
                     /plasmid="pF404-6"
     gene            complement(join(2080..2182,1..425))
                     /locus_tag="R8800_RS25235"
                     /old_locus_tag="FGAF404_48610"
     CDS             complement(join(2080..2182,1..425))
                     /locus_tag="R8800_RS25235"
                     /old_locus_tag="FGAF404_48610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_072650454.1"
                     /translation="MSVERYKSNPFLENMIVPVKGRQVKLSRLGKDNNILMNQSTGEFL
                     GTHVTTYKQVDADQFIKIFTDNIAMTFDFTSAGIKAFGVLLWAVQHHALSKDEVDLDSF
                     ILDAFISENNKENKKIRLSMATFKRGINELEKASIIAKTLRKGRYYINPNFVFNGDRIA
                     FTNLIERKAKDK"
                     /name="replication/maintenance protein RepL"
     gene            1613..2038
                     /gene="mobA"
                     /locus_tag="R8800_RS25240"
                     /old_locus_tag="FGAF404_48620"
     CDS             1613..2038
                     /gene="mobA"
                     /locus_tag="R8800_RS25240"
                     /old_locus_tag="FGAF404_48620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_064261972.1"
                     /translation="MVKNKRSGSETRQRSKVITLRINNKIDREIRRLAKAEGMTISEYI
                     RTSCLNKEIIQRIPSRYLDELLELGRMQKNLFDKGRTPKDKDYLDVMHKIITLCAEAKE
                     VILRNSDIYKEMDLLKQEIKIIKQYYKNKYPGSDLFN"
                     /name="mobA"
ORIGIN
        1 tttgctatta tacttgcttt ctcaagttca ttaatgcccc ttttaaatgt ggccatactc
       61 aaccttatct tcttgttttc tttattattt tcagagatga aggcatccaa tataaaacta
      121 tctaaatcaa cttcatcttt agataatgca tgatgctgaa ccgcccaaag aaggacacca
      181 aaagctttaa ttccggcgga agtaaaatca aatgtcatcg ctatattatc tgtaaatatt
      241 ttgataaatt gatcggcgtc gacttgttta tatgttgtca cgtgagtacc aaggaactct
      301 cccgtcgatt gattcattaa tatattatta tctttgccta aacgagataa tttaacctgc
      361 ctacctttga caggcacgat catattttcc aaaaatgggt tcgatttgta cctttcaacg
      421 ctcataatgc accattatat tgaaccttag tagctaaaaa agcgcccttt caaagctcat
      481 aatgaacgaa ctatttgagc tttgaaagct cacactatag ctaacacggc tcaaaaacaa
      541 cacctatttt gagccgtgtt ggtaacaggg ttgagctaac aagttacaga tccgaagatc
      601 acgatcccca tttcatgcgg gttgcagagg attcccttct tagttcttat acggtggtgc
      661 gtaagtgccc taggctgaca tctggttcac ttccgcttcc tcgcccactc gctcactaac
      721 gttcggtcgc tggggctgcg gcgagcggat cctcctctct cgtagtggct ggcttttggt
      781 gttgttcttt cgaagatgag tcgtcagcat ggccctaaaa cgcgctcaga gcctctcaaa
      841 cgcacgaaac ggggtttaaa tgcccgtgag ggcatggaag gcgcgaaaaa cgcttacaga
      901 gggtttcagg aggccaggcg cgccgatacc cctaactagg ggctttcagg tcgctaggaa
      961 ccgcgcaagc cccctgcaaa gcccaaccgc acgggacagg ccgccactcg gttggtgggt
     1021 ggtcgaagac cgttggtgtt acagcggcgg gggtctgggg gtctccccag acggtttccg
     1081 gtagcgctca aggcgctgtc ggaaccgtgg cgggtttttt ttaacaaaaa acgacaggtc
     1141 ggaaattaaa gcgcgaactg aacttatatt tcacatatat aaacgcgata gttatcatgc
     1201 acacacatat atattcgctt catcgttgta atctgtgcga tttctgcctt aatcgagcat
     1261 attgacggca tcggttaaca ctgaaaataa tactatcgcg aggattaaat aattatttag
     1321 catatatata ccgcagacga taagagaaga aacgtaatat caaataaaaa taataaaaaa
     1381 aataacaagc accattttaa aacctgtgta aaaaatattg cgtgatgcct tgcttttttg
     1441 tattgaagat accagcttat gaggtagttt tttattttcc gccgaaaagt tgtagcgaaa
     1501 aacgggacag gatgtgtatt tgtcataccg cgaatacatt cgcttgtatg aaaatacctc
     1561 ctgtcagggc aggccccgac accccgaacg tggaaaccag aggttcattg tgatggttaa
     1621 aaataagcgc agcggctcag aaacacggca gagaagcaag gttataacct tgcggataaa
     1681 taacaagatt gacagggaga tccgacgcct ggcaaaggcg gaagggatga cgatttcaga
     1741 atatataaga acgtcatgtt taaataaaga aataatacag cgcatccctt ccagatattt
     1801 ggatgaatta ttagagcttg gccggatgca gaagaattta tttgataaag gaagaacacc
     1861 gaaggataag gactaccttg atgtgatgca caaaataatc acgctgtgcg cagaagcgaa
     1921 ggaggttatt ttacgtaatt ctgatatata taaagagatg gatttattaa aacaggaaat
     1981 aaaaataatt aaacagtact acaaaaataa atacccaggc tctgatttat ttaattaaaa
     2041 taaaccctgc tcaattttta gagcggggtt tatatgttgt tatttgtctt ttgcttttct
     2101 ttctatcaga tttgtgaagg ctattctatc accattaaaa acaaagtttg ggtttatata
     2161 ataacgtcct tttcttaggg tt
//
