LOCUS       NZ_OY754369             3372 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F404 plasmid pF404-5, complete sequence.
ACCESSION   NZ_OY754369
VERSION     NZ_OY754369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113542961
            Assembly: GCF_963575385.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:01:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,048
            CDSs (total)                      :: 4,926
            Genes (coding)                    :: 4,730
            CDSs (with protein)               :: 4,730
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 79 of 196
            Pseudo Genes (incomplete)         :: 120 of 196
            Pseudo Genes (internal stop)      :: 36 of 196
            Pseudo Genes (multiple problems)  :: 39 of 196
            Pseudo Genes (short protein)      :: 3 of 196
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754369.1.
            Complete genomic sequence of E. coli strain F404.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F404"
                     /db_xref="taxon:562"
                     /plasmid="pF404-5"
     gene            complement(910..1101)
                     /locus_tag="R8800_RS25210"
                     /old_locus_tag="FGAF404_48570"
     CDS             complement(910..1101)
                     /locus_tag="R8800_RS25210"
                     /old_locus_tag="FGAF404_48570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1165..1488
                     /locus_tag="R8800_RS25215"
                     /old_locus_tag="FGAF404_48580"
     CDS             1165..1488
                     /locus_tag="R8800_RS25215"
                     /old_locus_tag="FGAF404_48580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1697..>2137
                     /locus_tag="R8800_RS25220"
                     /pseudo=""
     CDS             1697..>2137
                     /locus_tag="R8800_RS25220"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2166..2726
                     /locus_tag="R8800_RS25225"
                     /old_locus_tag="FGAF404_48590"
     CDS             2166..2726
                     /locus_tag="R8800_RS25225"
                     /old_locus_tag="FGAF404_48590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067486.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGMKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2733..2966
                     /locus_tag="R8800_RS25230"
                     /old_locus_tag="FGAF404_48600"
     CDS             2733..2966
                     /locus_tag="R8800_RS25230"
                     /old_locus_tag="FGAF404_48600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          746..829
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          1479..3049
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.628"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaccagttca ttcagtttgc atgagccttc tgtattcttc aagaattttt caacagcaca
       61 gtctcccagg aaccccgaaa aaaacctgtt tttgcagata cttgcgtatt tgcgagaatc
      121 gtttgcggtt gggggagctg tgacgacggc gaccgcacgc gtgagagttc cgctaccggc
      181 gcagagagtt tcatcgacag cggacacctg atgagttgcc acaaaaaccg tatgtagcgc
      241 ggctagattg gttgagttag accttggcgg cagttcccgg tatgtggtgg cgtgacgggt
      301 gagatggtgc ggcgaagacg catacgtcac gcaggcgcgg gttcttccac tgacgcgccc
      361 cgtgatcgat ggccttggct ccgtccggca tatcgagagc ggcaaactac ccgtcccttc
      421 actgggaggg gtgagttgtt gccgttccct ccgaccttcc accatccagc atatcgcacg
      481 atcccatgtg caccgctact cctgataact ccagtgcaaa aaaaaaacgg atctgcatca
      541 caacgagagt gatcccaccg ctcgccgcag ccccagcgac cgaacgtagt gagcgagtgg
      601 gcgaggaagc ggaagagtca cagaggcaag cattgtgcac ttacgcgcct gtgccagtgt
      661 ccgcgtgtgt gttgtttatc gggcgcgctg tttttacgca tctgtgcggc tttttactcc
      721 tgacatggtg cacttcccat ccactctgca ccgatgcaaa atgaggtaag ccagtataca
      781 catcgctatc gctctgtgaa ctgggtcagg gctgcgcccc gacacccgca ctgacgcgcc
      841 ctggcgggct tgtctgctcc cggcatccgc tcacaggcaa gctgtgaacg tctccgggag
      901 ctgcatgttt cagttgcttt caccgtcatc gccgaagcgt gcgaggcaac tgcggtagag
      961 ttcgtcggcg tggtcgtgaa gtgattcgca gatgtctgcc tgttcgtccg cgtccagatc
     1021 gttgagtttt tccagcaggg tcagcgtctg gcttctgata aagcgagcca tgttaagggc
     1081 tgttttttcc tgtttggtca tggtcttaag gctccttgta tgggggattt gttaggggga
     1141 atgataccga tgaaacgcga gaagatgctc acgatacggg ttaccgatga ggagcacgcc
     1201 cggttactgg aacgctgcga gggtaaacga ctggcggaat ggatgcggcg ggtctgtctg
     1261 ggggagccgg ttgcacgcac aggaaaactg ccgacactgg caccgccatt gctgcggcat
     1321 ctggctgcca tcgggaacaa cctgaaccag acagcccgga aggtgaacag cggccagtgg
     1381 tcttcgattg accgggttca tgtggtggcc gcactgatgg ccatcgaggg tgaattgcgc
     1441 cagttgcggc aggctgtcag ggaacagggg gtacgggatg atagttaaat ttcatgccag
     1501 ggggaaaggc ggtggaagtg gcccggtcga ttacctgctg gggcgggagc gtaaccgcga
     1561 aggcgcaacg gtgctccggg gtaacccgga agaagtgcgg gaactcattg attccacgcc
     1621 attttcgaag aaatacacgt caggcgttct gtcgttcgcg gagaaggagc taccaccggg
     1681 tgaacgcgaa agagtgatga cgagctttga gcgtgttctt atgcccggtc tcgaaaaaga
     1741 ccagtacagc attctctggg tggaacacca ggataagggg cggctcgaac tgaattttgt
     1801 catcccgaac atggagctac agagcggtaa gcgcctccag ccgtactacg accgtgcgga
     1861 caggccgagg ataaatgcct ggcagacgct ggttaatcat cattacgggc tgcgtgaccc
     1921 gaacgcaccg gagaacagac ggacgcttgt cacaccgaat aatctgccaa aagcgaaaca
     1981 ggaggccgca gaagcgatta cacggggtct tgaggcgctt taccatgccg gagcaataaa
     2041 aacgcgtcag gatgtcactg aggcgctcac agcggcaggt tttgaggtgg tgaggacaac
     2101 cagaagcagt atcagtattg ccgacccgga gggggggcgg aatctcaggc taaagggggc
     2161 gctgtatgag caatctttta caaatgggaa cagacttcga gaagaaactg aaagagcgag
     2221 ccgcttctac cgagaacatg ctgaacagcg agttcaggaa gctcgaagag tctgtagaca
     2281 aggcattgag cttaaacagg cagaaaatca gagacgctat cagcgaacat acaacatccg
     2341 tgaaaaagca actggatacg ctgagcacca ccgtgagcat gcagttcagc accacagagg
     2401 ccgaactgtc ccggcagcag aaaaaactgc tctggcgggt gatcaaaggg cggatactgt
     2461 ttccggcgct gaccgcgctc agcgtcacgg gcgggatttt cctggggtgc tgggggctga
     2521 tggagtggca ggaaagcaag attgcgaaaa atatcctgac tatcagagag caggaaaaca
     2581 cgctggcgaa actcgaggca aaaacctggg gggtgacgtt cgtcaatggc gagaacggga
     2641 aatttctggt gttgccggac gggatgaaag gggaaaacac ctggacggtg ggcgacaaga
     2701 acgccgtgag gctggtcagg gagtaaacga gtatgacgga actggaaggg catttgctga
     2761 acgccttaga gcatttgcaa caggactata tgcggcggct gaacgagtgg gagagcgcct
     2821 tcgcggaatt gcagaagatg cacgaggtta cgcaacggaa caacgcgata ctgaacgagc
     2881 gcgtcgtgat attgagtcag caggtgcaga gattagccgg gcaagtggat cgcttgagtc
     2941 ggttatttat cgcgaacaac agctaaggga tgaacaggag cgtgcccggt acagacatga
     3001 ggtacaggaa cgcgcggcac gggttcggag tgggccatca atggagatgt gaccggggaa
     3061 gtgacgaaat cttgccattt tgacccgttc gacggctggg gcgagcgaaa caaatcgtgt
     3121 gccgtatttg tgcatagcta tgcaacatcc tgaaaacgca tttcacgcgt ttttggggat
     3181 tcagccaggg gaaaacatgg tttttttact ttttgagcgt aaagacgtga aaaatctcgt
     3241 tattgcactt gcgatctttc gtcggcgggc gtagaacgaa aacagctcgc caggaaggcg
     3301 agctgtttcg ggttcattga ggtctctgac ctcctcaatg ttcctgtgga aactgttgag
     3361 atccacaaca ga
//
