LOCUS       NZ_OY754362             6074 bp    DNA     circular CON 01-NOV-2023
DEFINITION  Escherichia coli isolate F1022 plasmid pF1022-5, complete sequence.
ACCESSION   NZ_OY754362
VERSION     NZ_OY754362.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA113543002
            Assembly: GCF_963575365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Leclercq,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2023) INRAE, ISP, Centre INRAE Val de Loire, Site
            de Tours, Bat 213, 37380 Nouzilly, France
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/01/2023 10:22:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,486
            Genes (coding)                    :: 5,131
            CDSs (with protein)               :: 5,131
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 355
            CDSs (without protein)            :: 355
            Pseudo Genes (ambiguous residues) :: 0 of 355
            Pseudo Genes (frameshifted)       :: 126 of 355
            Pseudo Genes (incomplete)         :: 254 of 355
            Pseudo Genes (internal stop)      :: 76 of 355
            Pseudo Genes (multiple problems)  :: 90 of 355
            Pseudo Genes (short protein)      :: 4 of 355
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY754362.1.
            Complete genomic sequence of E. coli strain F1022.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="F1022"
                     /db_xref="taxon:562"
                     /plasmid="pF1022-5"
     gene            1..915
                     /locus_tag="R8966_RS27970"
                     /old_locus_tag="FGAF1022_54420"
     CDS             1..915
                     /locus_tag="R8966_RS27970"
                     /old_locus_tag="FGAF1022_54420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032333168.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1119..1517)
                     /locus_tag="R8966_RS27975"
                     /old_locus_tag="FGAF1022_54430"
     CDS             complement(1119..1517)
                     /locus_tag="R8966_RS27975"
                     /old_locus_tag="FGAF1022_54430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1694..2620
                     /locus_tag="R8966_RS27980"
                     /old_locus_tag="FGAF1022_54440"
     CDS             1694..2620
                     /locus_tag="R8966_RS27980"
                     /old_locus_tag="FGAF1022_54440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_114419937.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WAWDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPEWGGAKKDSGSLT
                     LTQQKEQLRELRKRWEMKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2623..3156
                     /locus_tag="R8966_RS27985"
                     /old_locus_tag="FGAF1022_54450"
     CDS             2623..3156
                     /locus_tag="R8966_RS27985"
                     /old_locus_tag="FGAF1022_54450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            3378..5105
                     /locus_tag="R8966_RS27990"
                     /old_locus_tag="FGAF1022_54460"
     CDS             3378..5105
                     /locus_tag="R8966_RS27990"
                     /old_locus_tag="FGAF1022_54460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_136698891.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGHGNGGGNGNSGGGSNSSVAAVAFGFPALAAPGAGTLGISVSGE
                     ALSAAIADIFSALKGPFKFSAWGIALYGILPSEIAKDDPKMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTALPRGITQDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPVYVSV
                     TDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTNVN
                     NKKAAFDAAAKEKSDADVALSSALERRKQKENKEKDAKAKLDKESKRNKPGRATGKGKP
                     VNNKWLNNAGKDLGSPVPDRIANKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFSRNNN
                     DRMKVGKAPKTRTQDVSGKRTSFELHHEKPISQNGGVYDMDNISVVTPKRHIDIHRGK"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5107..5370
                     /locus_tag="R8966_RS27995"
                     /old_locus_tag="FGAF1022_54470"
     CDS             5107..5370
                     /locus_tag="R8966_RS27995"
                     /old_locus_tag="FGAF1022_54470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_001560791.1"
                     /translation="MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKIT
                     EHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5432..5575
                     /locus_tag="R8966_RS28000"
                     /old_locus_tag="FGAF1022_54480"
     CDS             5432..5575
                     /locus_tag="R8966_RS28000"
                     /old_locus_tag="FGAF1022_54480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001560790.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6074
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..776
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.268"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1695..2617
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.13"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgacgaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacata
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgaaggggca
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa aaatcctgtg
      241 aacggtcatg ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc gccggacagc
      301 tcggttaagg cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt
      361 gctgatgtga actacagcgg actgatttgt aaaaatccgt tccacctgga atggcaggtg
      421 atggagtggc gagaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctga attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tcaccgcctg aatgtcgggc tattggcagg agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca caccagaaat tcaggctgca
      781 cgcggtcgca agggcggttc taagtctaag cgcagcacag tggctacatc tgcacgcacg
      841 ctgaaaccgt gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttt tggcgtcttt
      961 ttgagcagcc ggttttttgc cgtttttccc cgttcagccc tgcaatggtg ctgttttccg
     1021 catgattgcg ctggtagtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcac cagggaatcg gaacacggat ggaggcactt acgcgtctgg gtttggggat
     1141 gtctccagat cgtacttaag ttccttaagt gcctgtaata tcctgtcttt tttatagcat
     1201 agctcgcctg tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag
     1261 agccggtgat tgcgttctcg tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt
     1321 tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggagg
     1381 agtttgcgtt cctgttctgt gagtcctgct tcgttgtcga gcaggtcaat aatctgtctc
     1441 tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt cagccattcg
     1501 atgttgctga tgcgcatgtt cgtggtgctc cctggtgttt tttgcggtgt gttgtggtcg
     1561 tgcagtatat cgcttgcggt cggtgggtgc aggggggtat cttccgtgct aattcgtaaa
     1621 attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg cgctcctgcg
     1681 gagggctgaa accgtggcaa cctatcacct cagcgtcaaa tttggaggca agggacaggc
     1741 tgcaaaccac gccgattaca tcgagcgcaa aggggagtat gcgaaacgga aagatctgga
     1801 atacacggag cacggaaaca tgccggaatg ggcctgggat aatccttccc atttctggca
     1861 ggcggctgac cagtttgaac gtgcgaacgg ttcgacttat cgggaactgg aaatcgcgct
     1921 gccccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg tacgtcagga
     1981 agccggcgaa cggcacgcct ggagttttgc cattcacaat ccgaaagcga gcattgacgg
     2041 cggcgaacag cctcatgcgc atatcatgat gagccagcgt gtgaatgatg gcattgaccg
     2101 tacaccggaa cagtatttcc ggcgctataa cgccaggtat cccgagtggg gaggtgcgaa
     2161 aaaggattcc ggaagcctga ctctcactca gcaaaaagag cagttgcggg aattgcgaaa
     2221 acgctgggag atgaagcaca acgaacatat gagaaagcat ggctttgaaa ggggcttcat
     2281 tgactgccgg acgctgaaag aacagggcat tgaacgacgt cccgaagtgc atctgggttt
     2341 tgaagctgcc ggacggcttg atgatgccca gcgtcatgac atcatgcaaa aacggtctga
     2401 accggactat tcccgccatc ttgaacaggc gcgaacggtc gggcatgaca tcagggaatg
     2461 gctggacact ccggttaagc aacatgaact ggtcagaagg cttcaacatg atgttgcgcg
     2521 ggagcatggc tttgacatga gagacagagt gagcagacag gaccgaactg aacctgaacg
     2581 ggttaaatca ccgtcacgtg gattttctat gggattctga acatggccag cacagaagaa
     2641 aaattactca cgctcctgtt atcggtggag catctgcaac ataccgcaat ggagcaacag
     2701 aaagctgttg ctgattctct ggctgtggca agggaaacac ttcgcggata tcgggatatt
     2761 tctgacggtg tggcaaaaca ggtcagggat gaagtcagaa acgaggtgca gaagctggat
     2821 gttgcagggc tgataggtga gcgcatcagc caggattttc agcggctgga agagtcagtc
     2881 gcaaacatga aggcaaacgc ggatgccctg aacagggata ttgcgacaat tcacagcaat
     2941 atcgtgcagg aatacaacac ccttaaaggg tatagctgga aatggctggg tggggtgtca
     3001 gtggcttttc tggttatgtt ctgtgtgctt ggatggggaa tgaaaacaat gctcgacagg
     3061 aactacaacg ccacagcggc ggtgtatcag aaagttgtga cactggaaga taaactggcg
     3121 gagaaacctg tcacgggcaa gagccggaaa aactgacgcg cttcgcttgt ccaacgcctc
     3181 acgacccgcg aaaggatttc gcgttccgtt caccgttttc atgagtgatt cctccgaact
     3241 gccaaaaaat atcccctccc tgacttgaca gaaaaaataa tggcgagtac tttttgatct
     3301 gtacataaaa ccagtggttt tatgtacagt attaactatg taattaattg ttttaacgct
     3361 taaaagaggg aatttttatg agcggtggag atggacgcga ccacaacagt ggcgcacata
     3421 acacaggtgg taacattaat ggcggcccta cggggcttgg tggaaatggt ggggcttctg
     3481 acggctccgg atggagttcg gaaaataacc catggggtgg cggttccggt agtggtgttc
     3541 actggggagg tggctccggc catggcaatg gcggggggaa tggtaattcc ggtggtggca
     3601 gcaattcatc cgtagcagca gtggcatttg gttttcctgc tttggctgct cctggtgccg
     3661 gaactcttgg tatttccgta tctggcgagg ctttgtctgc ggcaatagcg gatatctttt
     3721 cagccctgaa aggcccgttt aaattcagtg catggggtat tgcgctttat ggcattctgc
     3781 catctgaaat agcaaaagat gacccgaaaa tgatgtcaaa gattgtgacg tcattaccgg
     3841 cagaaacagt aacaaatgtt caggtcagta cacttccgct ggaccaggca actgttagcg
     3901 tgacgaaacg tgtgaccgat gttgtgaagg acacacgaca gcatattgcc gtagttgcag
     3961 gtgtacctat gagtgtgcct gttgtgaatg ctaaaccaac acgtactcca ggtgtatttc
     4021 acgcatcatt tcctggtgtc ccttctctga cagtcagcac tgtcaaaggc cttccggtgt
     4081 caacagccct tccccgtggt attactcagg ataaaggccg gactgccgtt cctgcgggat
     4141 ttacctttgg tggtggttca catgaagcgg tgattcgctt tccgaaagaa agtgggcaga
     4201 agccggttta tgtatcagtg acagatgttc ttaccccagc acaggtaaaa caacgtcagg
     4261 atgaggaaaa acgcctccag caggaatgga atgacgcaca tccggtggaa gtggctgaac
     4321 gcaattatga acaggcccgc gcagaactga atcaggctaa taaagatgtt gccagaaacc
     4381 aggaacgaca ggctaaagct gttcaggttt ataattctcg taaaagtgaa cttgatgcag
     4441 cgaataaaac tcttgctgat gcaaaggctg aaataaaaca attcgagcga tttgcccgag
     4501 aaccaatggc tgctggtcac agaatgtggc aaatggcagg gcttaaggcc cagcgggcac
     4561 agacgaatgt aaataataag aaggctgcat ttgatgctgc tgcaaaagaa aagtcagatg
     4621 ctgatgttgc attgagttct gcgttggagc gccgcaaaca gaaagaaaat aaagaaaagg
     4681 acgctaaagc caaattagat aaggagagta aacggaataa gccagggagg gcaacaggta
     4741 aaggaaaacc tgtcaataat aagtggttaa ataatgcagg taaagactta ggttctcctg
     4801 ttccagatcg tatagctaat aaactacgtg ataaggagtt taagagtttc gatgattttc
     4861 gtaagaaatt ctgggaagaa gtgtcaaaag atcctgagtt aagtaaacaa tttagtagga
     4921 acaataatga tcgaatgaag gttggaaaag cgcccaagac tagaacccag gatgtttcag
     4981 ggaagagaac ttcattcgag cttcatcatg agaagccgat cagccaaaat ggtggtgtct
     5041 atgatatgga taacatcagc gtggtaacac ctaaaaggca tattgatatt caccgaggta
     5101 aataatatgg aactgaaaaa tagtattagt gattacacag aggctgagtt tgttcaactt
     5161 cttaaggaaa ttgaaaaaga gaatgttgct gcaactgatg atgtgttaga tgtgttactc
     5221 gaacactttg taaaaattac tgagcatcca gatggaacgg atctgattta ttatcctagt
     5281 gataatagag acgatagccc agaagggatt gtcaaggaaa ttaaagaatg gcgagctgct
     5341 aacggtaagc caggatttaa acagggctaa aatatgaatg ccggttgttt aaggatgaat
     5401 gactggcatt ctttcacaac aaggagtcgt tatgaaaaaa ataacaggga ttattttatt
     5461 gcttcttgca gccattattc ttgctgcatg tcaggcaaac tatatccgtg atgttcaggg
     5521 cgggacagta tcaccgtcgt caactgctga actgaccgga gtggaaacgc agtaacctgc
     5581 aatcctcatt tgacaagaac aaagcgtgtc aggctgattc tgacgcgctt tttttgttga
     5641 agtgccacaa aaattccatg tgggagatgg gatctaaaat ctccgtgcag aaccttccat
     5701 ccggagggag aaaacttttc gttttgagcc gttcggtgtt cagaacgcac gaaaccgatc
     5761 gcgcatcgct ttcgtgcata gttatgcaac gcgctaaaaa cgattctgac gcatttttct
     5821 ggtttagcct ggtgtttcct tgtctttttg cgttttttgc gtcaggaagc gtctgagagc
     5881 gttttaaggg gtacgtacaa cgggagttat ggtaaatgga tcgggttttc gggaaggttc
     5941 gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc ctgaaatcat
     6001 gtccagatcc ggcaagattc aacatgaaat acagagggcg tgtaggtcgc ataacccgga
     6061 gagaatcgta acac
//
