LOCUS       NZ_OY725783             8632 bp    DNA     circular CON 06-DEC-2023
DEFINITION  Parabacteroides distasonis strain Parabacteroides distasonis
            TSDC17.2-1.1 plasmid 38, complete sequence.
ACCESSION   NZ_OY725783
VERSION     NZ_OY725783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA114266242
            Sequence Read Archive: ERR11836198, ERR11836210
            Assembly: GCF_963360645.1
KEYWORDS    RefSeq.
SOURCE      Parabacteroides distasonis
  ORGANISM  Parabacteroides distasonis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
            Parabacteroides.
REFERENCE   1
  AUTHORS   Beller W,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2023) WASHINGTON UNIVERSITY CENTER FOR GENOME
            SCIENCES, Jeffrey I Gordon Lab, 4523 Clayton Avenue, MSC
            8510-41-4209, St. Louis, MO 63110, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/06/2023 16:00:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,487
            CDSs (total)                      :: 4,412
            Genes (coding)                    :: 4,304
            CDSs (with protein)               :: 4,304
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 108
            CDSs (without protein)            :: 108
            Pseudo Genes (ambiguous residues) :: 0 of 108
            Pseudo Genes (frameshifted)       :: 32 of 108
            Pseudo Genes (incomplete)         :: 69 of 108
            Pseudo Genes (internal stop)      :: 22 of 108
            Pseudo Genes (multiple problems)  :: 13 of 108
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OY725783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Parabacteroides distasonis"
                     /mol_type="genomic DNA"
                     /strain="Parabacteroides distasonis TSDC17.2-1.1"
                     /db_xref="taxon:823"
                     /plasmid="38"
                     /geo_loc_name="USA"
                     /collection_date="2021-09"
     gene            156..722
                     /locus_tag="UNS60_RS16720"
                     /old_locus_tag="PDTSDC17_04482"
     CDS             156..722
                     /locus_tag="UNS60_RS16720"
                     /old_locus_tag="PDTSDC17_04482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="WP_005868760.1"
                     /translation="MAHQSKYPSLDRQWHKMMFSFFEYLPMQYRQATEREWQIRKMIWS
                     FKDGKAYLNIAWMIANKLHQVFGDDVKNIVFACVPASSADKNELRYKGFASAVCKFSGA
                     INAYEHIRVSGDRLAIHEKFDSKSLQKVQVIEFDKDFFRGKKILVFDDILTKGFSYARF
                     ACQLEKIGGEVLGGFFLGKTVVRML"
                     /name="phosphoribosyltransferase"
     gene            724..1338
                     /locus_tag="UNS60_RS16725"
                     /old_locus_tag="PDTSDC17_04483"
     CDS             724..1338
                     /locus_tag="UNS60_RS16725"
                     /old_locus_tag="PDTSDC17_04483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="WP_005868759.1"
                     /translation="MKEKESYIEKQKDIFGDTTWFTYRYEVNGMVYETSAGSLDICRKA
                     RDKWMKMMSVAFTGHRTIRTNKYALSVSLNEEVRFCYENGIRFFYIGCAVGFDMMAAHT
                     VLEQRKQYPDMVLVAVVPYVGQDVYFNKEDKQRYADILRQADKVVVLSEYYYAQCYAHR
                     NDYMISHACRLIAYWDGKSAGGTSYTFNKAQKKKLVIYNLF"
                     /name="SLOG family protein"
     gene            complement(1532..2695)
                     /locus_tag="UNS60_RS16730"
                     /old_locus_tag="PDTSDC17_04484"
     CDS             complement(1532..2695)
                     /locus_tag="UNS60_RS16730"
                     /old_locus_tag="PDTSDC17_04484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568430.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="WP_005868407.1"
                     /translation="MFQDKYVFAQLASFLNRSKFNRIVTKYDGDKYVKHFTCWNQLLAL
                     MFGQLSNRESLRDLIVALEAHHSKCYHLGMGKNVSKSSLARANQDRDYHIFEEYAYYLV
                     SEARQKCANHIFKLGGNVYAFDSTTIDLCLSVFWWAKFRKKKGGIKVHTLYDVETQIPA
                     FFHITEASVHDSKVMIEIPYEPSSYYIFDRGYNNFKILYKIHQIEAYFVVRAKKNLGYK
                     SIQWKRRLPKNVLSDASVFLTGFYPKQYYPEPLRLVKYWDEEQEREFTFITNAMHISAL
                     QVAELYKNRWQVELFFKWLKQHLKIKRFWGTTENAVRIQIYAAICAYCLVAIIQHDMQL
                     NRSTYEVLQILSISLTDKTHLRDLFDKTKFQNDKERFGPNGPSLFNF"
                     /name="IS4 family transposase"
     gene            3132..3581
                     /locus_tag="UNS60_RS16735"
                     /old_locus_tag="PDTSDC17_04487"
     CDS             3132..3581
                     /locus_tag="UNS60_RS16735"
                     /old_locus_tag="PDTSDC17_04487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="WP_005868755.1"
                     /translation="MEITHKNQGELDSTMLPFVMRELVELVMKKKALPLGDALYYIYSS
                     KLYKSLLDKSTKLWYSSTLSLYETLEKEKTEEKRRYNGDTKILLFKMFCIENYREEKKQ
                     SAEETLLLFSDYGVFDFLDETFEMLHTQDPEYILDTITTYINKRK"
                     /name="DUF3791 domain-containing protein"
     gene            3578..4060
                     /locus_tag="UNS60_RS16740"
                     /old_locus_tag="PDTSDC17_04488"
     CDS             3578..4060
                     /locus_tag="UNS60_RS16740"
                     /old_locus_tag="PDTSDC17_04488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="WP_005868753.1"
                     /translation="MRLFHGSTVTVKRPNIQKGRKATDFGKGFYTTTNFEQAKKWALLK
                     KNREQSEKAIVSVYEVPDDILDREYPVLRFMGATKEWLEFVVNNRRGRENGDYDLIMGP
                     VANDQLYATIRLYEQRVVTAEAAIEMLKAHKLFNQLSFHTVTVALLLKFVESIEVG"
                     /name="DUF3990 domain-containing protein"
     gene            complement(4405..4602)
                     /locus_tag="UNS60_RS16745"
     CDS             complement(4405..4602)
                     /locus_tag="UNS60_RS16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007564664.1"
                     /translation="MKISNRFGKDAKNNRLDSKHGTSRKDGQPPIDTPEETSAIPFKKM
                     KPKNEAYHIPRIEKSDCTIK"
                     /name="hypothetical protein"
     gene            4934..5269
                     /locus_tag="UNS60_RS16750"
                     /pseudo=""
     CDS             4934..5269
                     /locus_tag="UNS60_RS16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781195.1"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /name="single-stranded DNA-binding protein"
     gene            5439..5777
                     /locus_tag="UNS60_RS16755"
                     /old_locus_tag="PDTSDC17_04490"
     CDS             5439..5777
                     /locus_tag="UNS60_RS16755"
                     /old_locus_tag="PDTSDC17_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_005868750.1"
                     /translation="MKRLYDTYTIYEFSDECIVKEICKRVKSIRLSCCFSQQEFADKAG
                     VSIVTIKRIESCKVSDIALSTLLKILRISGTLEGVVGLVPELPDSPFLINEKTGKRIQR
                     INGKRKMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5774..7051
                     /locus_tag="UNS60_RS16760"
                     /old_locus_tag="PDTSDC17_04491"
     CDS             5774..7051
                     /locus_tag="UNS60_RS16760"
                     /old_locus_tag="PDTSDC17_04491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin"
                     /protein_id="WP_005868749.1"
                     /translation="MKKEPIIVNVYLWGTCIGKLNWDFEKHCSVFQFTDEYRKQDYDIC
                     PSTHPKRTPLFASFYGNKDKLYQGLPEFLADALPDKWGASLFDQWLTDNNIKVTESLPL
                     LKLSYIGKRAMGALEFEPEFNDDAIHESVNMSSLATLASKIYNDRDAAVISPEDSLTMK
                     KLIYLGTSAGGMRPKAVIAYNLETEEFRSGQVDLPENFKQYIIKFKEADDSPTTEIEMV
                     YSEMAKAAGINMMPCFLKEIDGRNHFVTERFDRKNGEKLFSQTLAAIMPGADDYMKLCW
                     IAETLKLPQEDKDQIFIRMVFNYVAGISDDHNKNISFIMDKAGVWRLSPAYDVMFTANT
                     WENSSAHIHSMGVMGKRSALTTSDFVNFAEDFVEEPEKKILQVFDAVSKFQSLCATYGI
                     DKAIFDKIQHVLDGLVTDDLDLLQLT"
                     /name="type II toxin-antitoxin system HipA family toxin"
     gene            complement(7209..7370)
                     /locus_tag="UNS60_RS16765"
                     /old_locus_tag="PDTSDC17_04492"
     CDS             complement(7209..7370)
                     /locus_tag="UNS60_RS16765"
                     /old_locus_tag="PDTSDC17_04492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005868748.1"
                     /translation="MGKQQTAGQIKGEWHSRRKEVVSMWPKLRKQTGYASLLCLPHSWI
                     SHNHGACE"
                     /name="hypothetical protein"
     gene            complement(7589..7786)
                     /locus_tag="UNS60_RS16770"
     CDS             complement(7589..7786)
                     /locus_tag="UNS60_RS16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_007564676.1"
                     /translation="MKISNRFGKDAKNNRSDSKHGTFRKYGQPPIDTPEETSAIPFKKM
                     KPKNEAYNIPRIEKSDCTIK"
                     /name="hypothetical protein"
     gene            8118..8516
                     /locus_tag="UNS60_RS16775"
                     /old_locus_tag="PDTSDC17_04493"
     CDS             8118..8516
                     /locus_tag="UNS60_RS16775"
                     /old_locus_tag="PDTSDC17_04493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_005868746.1"
                     /translation="MKKIENSFAVTGFIGKDAEIRSFETASVARFSIAVSRADKTGEET
                     SYVSAFMGIEAWRKNEALDSFDVLKKGELITVEGYFKPEEWTDSKSGEKRNRIVMVATK
                     FYPAPEKEEEKPDQPKQKAVSKKKKASK"
                     /name="single-stranded DNA-binding protein"
ORIGIN
        1 atgcgtgccg taaaaaaaag gctcataaac tcagatgggg catgaaagct caaaaaaaaa
       61 gtcggcagaa agaaaaggcc atcccggaaa ggggtttaaa aaaaggtctt taaatctagc
      121 tgaagcataa gtttaatttt aatagataaa agactatggc acatcagagt aaatatccta
      181 gtttagacag acaatggcat aagatgatgt tttctttctt tgagtatctt cctatgcaat
      241 atcggcaggc aacggaaaga gaatggcaaa ttcgtaagat gatatggagt tttaaggatg
      301 gaaaagctta tttgaatata gcttggatga tagcgaataa gttacatcaa gtttttggtg
      361 atgatgttaa gaatattgtt tttgcttgtg ttccggcttc ttccgctgat aagaatgaac
      421 ttcgttataa ggggtttgct tcggctgtgt gtaagttttc aggtgctatc aatgcgtatg
      481 aacatatacg tgtcagtgga gatcgtttgg ctattcacga gaaatttgac agcaagtcgc
      541 ttcaaaaagt acaagtgata gaatttgaca aggacttttt tcggggtaag aaaatcttgg
      601 tatttgacga tattttaaca aaagggtttt cgtatgctcg tttcgcatgt cagttggaaa
      661 aaataggagg tgaggttttg ggaggtttct ttttaggtaa aacagttgtc cgtatgttat
      721 aagatgaaag aaaaagagag ttatatagag aagcagaagg atatctttgg agatactact
      781 tggttcactt atcgttatga ggtgaatgga atggtgtatg aaacttcggc aggttcgttg
      841 gatatatgta gaaaggcacg tgataaatgg atgaaaatga tgtccgttgc ttttaccggt
      901 catagaacga tacgaacaaa taaatatgct ttgtccgtat cgttgaatga agaggtacgt
      961 ttctgctatg agaatggaat ccgttttttc tatattggtt gtgcggttgg atttgacatg
     1021 atggctgctc ataccgtact tgaacaaagg aaacaatatc ccgatatggt tttggtggcg
     1081 gttgttcctt atgtcgggca agatgtgtat ttcaacaaag aggacaaaca gaggtatgct
     1141 gatattttgc gccaagccga caaggtggta gttctatccg aatattatta tgcccaatgc
     1201 tatgcccacc gtaatgatta catgatttca cacgcctgta ggctgattgc ctattgggat
     1261 ggaaaatctg ccggaggaac ttcttatacg tttaacaagg cacaaaaaaa gaaattggtg
     1321 atatacaatc tgttttgatt cagattgtat atcgttgttt tccagcggta tgatcaaacg
     1381 tggcgatgtc atactgtctg gttgctgtaa gtgctaatac ttccgatgca aaagagaaag
     1441 acacaataaa tatgaaaagc aatgatagat gaattttact taattgtttt acctttgtaa
     1501 aacattagtg tcccattaaa attgggacga attaaaaatt aaataaactt ggcccatttg
     1561 gtccgaatcg ttctttgtca ttttgaaatt tagttttatc aaagaggtct ctcagatgag
     1621 tcttatcagt caatgagatg ctcagtattt gtaacacttc atatgtactt ctgttcagtt
     1681 gcatatcgtg ttgaatgatt gccaccaaac agtaagcgca tatagcagca tatatctgta
     1741 ttcgaacagc attctctgta gttccccaaa atcttttgat tttaaggtgc tgcttgagcc
     1801 atttgaaaaa cagctctacc tgccagcgat ttttataaag ttcagcaact tgaagcgcag
     1861 atatatgcat cgcattggtt atgaatgtaa attctcgttc ttgttcttca tcccaatatt
     1921 taaccaatct aagtggctct ggataatatt gtttaggata gaatcctgtc agaaatacac
     1981 ttgcgtctga aagcacattc ttaggcagcc tacgtttcca ttggatggat ttgtacccga
     2041 gattcttttt tgctctgaca acaaagtagg cttcaatttg atgaatttta tacagtattt
     2101 tgaagttgtt ataaccgcgg tcaaagatgt aataagagct tggttcataa ggaatttcaa
     2161 tcataacttt agaatcgtgt acggatgctt ccgtgatatg aaagaatgca ggaatctgtg
     2221 tttccacatc atataatgta tgcactttga taccaccttt ctttttgcgg aattttgccc
     2281 accaaaagac tgaaaggcac aggtcaatag tagtcgaatc gaaagcataa acgttaccgc
     2341 caagtttgaa aatatgatta gcacactttt gtcgtgcttc gctaaccagg tagtaagcat
     2401 attcttcaaa gatgtgatag tctctatctt gatttgctct tgccagcgat gactttgata
     2461 catttttacc cattcctaaa tgataacatt tggaatgatg agcttcaaga gcaactatca
     2521 aatctcgcag actttcacga ttagaaagtt gaccaaacat caaagcaagt agttgattcc
     2581 agcaggtgaa gtgcttcaca tatttatcac catcatactt ggtgactatg cggttaaact
     2641 tacttcgatt cagaaatgaa gccaattgag cgaaaacgta tttgtcttgg aacatatgcc
     2701 atcggattaa tctgatgcaa agctacaaat tcaagtccgt tcgctcggaa aatcgctgta
     2761 actaactaaa tttcaaatat ttcaaagaac tcttttagat tttaatggga caacaatgtt
     2821 tgtaaaaggt aaaacaatat gcgtttttca gatacagaga acaatatgac cttttgcata
     2881 ggtgtgtgtt ggttaggaac tccaaatatg gagtcgttgc agaaaataat gatgaacttg
     2941 aatattattg ataaggatgg aaacaatgga agctaatgaa ctgatgcgat atgcctatga
     3001 attactgcaa gggcattcga atgaagaatg tgcccgaata ttaagagaag tggtggccga
     3061 gtgtcaggtg tgtataaaga actgtgaaga aggtgttttc gaaaatatgc taaagtaata
     3121 acaacaagaa tatggaaatt actcataaaa atcaaggaga acttgactca accatgctcc
     3181 cattcgtgat gagagagctg gtagaactgg tcatgaagaa aaaggcattg cctttaggcg
     3241 atgcactgta ttatatatat tcttccaagt tgtataaatc tttgctggat aagagtacca
     3301 agttatggta ttcaagtaca ttgtcgcttt atgaaacatt ggagaaggaa aaaacagaag
     3361 aaaaacgacg gtataacggt gacacgaaga tcctgctttt caagatgttc tgcattgaga
     3421 attataggga ggaaaagaaa caaagtgcgg aagaaacctt gttgttgttt tcggactatg
     3481 gggtttttga ttttttggat gagacttttg aaatgcttca tacccaagac cccgaatata
     3541 tattagatac tataacgact tatatcaata aaagaaaatg agactatttc atggatctac
     3601 agttaccgtg aaacgcccta atatacagaa aggacggaag gctacggact ttgggaaggg
     3661 attttatacg acaaccaact ttgaacaagc aaaaaaatgg gctttgttga agaagaacag
     3721 ggaacagagc gaaaaagcaa tcgtctctgt ttatgaagtt cccgatgata ttcttgatag
     3781 ggaatatccg gttcttcggt ttatgggtgc gacaaaagaa tggctggagt ttgttgtcaa
     3841 caaccgcaga ggaagagaaa acggtgacta tgacctaata atggggcctg tcgcaaatga
     3901 ccagttatat gcgacaattc ggctttatga acagagagtt gttacggctg aagccgctat
     3961 tgagatgctg aaagcgcata agttgttcaa tcagctttca tttcatacgg taacagtcgc
     4021 tttgttgttg aagtttgttg aatcaattga agtggggtaa agaatatgtg aagtgaaaat
     4081 agggggaagt ttatccccca atggtaaggt tgttttttgc gtccacttag ttttttccca
     4141 cccacccttt cagcggagtg actgcccggc agtctgttgc ttacccatat gtcctagcct
     4201 tttagggctg tagataggta gttggatatt tcgctgccaa atcggcagtc cgcttcaatc
     4261 gtttgcttgc ttttctccga gtggagcctt tctgcttttc cggtggtgta atcttctttt
     4321 attggaaaca ggtgtagtgg ctgtatgcct gtgtggttat gttctcccgc agaatccgca
     4381 agtcagttct tccgttgcag cttttcattt tatcgtgcag tcactttttt cgatacgtgg
     4441 aatatgatat gcttcattct tcggcttcat ttttttaaaa ggaattgcag atgtttcttc
     4501 cggtgtgtcg ataggcggct gaccatcttt tcgggatgtg ccatgcttgc tgtctaaccg
     4561 gttgtttttt gcatcctttc caaacctgtt actgattttc aagttttgtc tttttcatat
     4621 cattttttag ggaagtcaaa tcattttccg ttacaaatat agattgttgc taaccccttt
     4681 tacggggata aggggacgat tcacaaacac cacgttgtta ttcgtctgaa atcttccttt
     4741 ttttgccttg cacaaaaagc gtatttaacc cgaattattc gttgccatct tcaccaataa
     4801 aaaagttaca aatcttcctt tttccggcca gcataaaaag agtatttata tcttttttca
     4861 tggtgtcccc caaagccaac aatcagttat tgcactgtaa aattgattaa ttaacttcta
     4921 aaaaatcaga gatatgaaaa agattgaaaa ttcattcgca gtaacaggca ggatttgtag
     4981 ggatgcagaa atccatttat tcgaaatagt aaaggttgca cacttctcta ttgtggtcag
     5041 tcgcataggc aagataaatg aagaaaaaac atttgtttcc tctgttatga aagttgaagc
     5101 ctagagtaag atggaagacc gctatgcttt ttggatactg caaaaagatg tactgattac
     5161 cgttgaaggc tactttaagc cggaagaatg gacggattct aaaagtgggg agaaacgtaa
     5221 ccgcattgtg atggtagcta ctaagttcta cccgactccg gaaaaataag aacccccgga
     5281 ctaaaaagaa aaagcaatca aacgattgaa gcggcacaag gtcgcttcaa tcgtttgcta
     5341 atgcggcaga agattataaa aaaagtgttt cttaatttgg tgattttcag atttatcggt
     5401 atttttgtat caaaaatgat acctaaatat gtttttctat gaaaagatta tatgatacat
     5461 atacaatata cgaattttca gatgaatgta ttgtaaagga aatctgcaag agagttaagt
     5521 ctataagact tagctgctgt ttttcccagc aagaatttgc ggataaggca ggagtatcta
     5581 tcgttactat taagagaata gaatcttgta aagtaagtga tattgcccta agtacattgc
     5641 ttaagatttt gcgtataagt ggaactcttg aaggcgttgt cggtttggtt ccggaacttc
     5701 cagactcccc tttcctaatc aatgaaaaaa caggtaaaag aatacaaaga attaacggta
     5761 aacgtaagat ggtatgaaga aagagccgat tatagtgaat gtatatctgt gggggacttg
     5821 cattggcaaa ttgaattggg actttgaaaa gcattgttct gtattccaat ttacggatga
     5881 atataggaaa caagattatg atatatgccc ttccacacac cctaagagaa cccctttgtt
     5941 tgcgtctttc tatgggaata aagataaatt atatcaaggg cttccggaat ttcttgcaga
     6001 tgcgcttcct gacaaatggg gggcttccct atttgaccaa tggctcacgg ataataatat
     6061 aaaggttacg gaatcattgc ctctattgaa gctctcttat attggcaaga gggctatggg
     6121 agcattggaa tttgaaccgg aatttaatga tgatgccatc catgagtcag taaatatgtc
     6181 atcattggca actttggctt ctaaaatata taatgatcga gatgctgctg tcatatcacc
     6241 ggaggatagc ctcacgatga aaaagttgat atatcttgga acttctgccg gtggcatgcg
     6301 cccgaaggca gtgattgcct ataatctcga aacggaagag tttcgttccg gccaggtgga
     6361 tttgcctgaa aattttaagc agtatatcat caaatttaaa gaagcagatg actctcctac
     6421 cacagagata gaaatggtat atagcgaaat ggcaaaggcg gcgggaatca acatgatgcc
     6481 atgcttcctg aaagaaattg atggaagaaa tcatttcgta acggaaagat ttgatcgtaa
     6541 gaatggagaa aagctcttta gtcagaccct tgcggccatt atgccgggtg cagatgatta
     6601 tatgaagctg tgttggatag cagagactct gaaacttccc caggaagaca aagatcagat
     6661 atttatcaga atggtattta attatgtcgc aggtatatcc gatgatcata ataagaacat
     6721 atcgtttatc atggataagg caggggtatg gcgtttatca cctgcatatg atgtaatgtt
     6781 cacggcaaat acatgggaaa attcttcagc acacattcat tcgatgggcg tgatgggcaa
     6841 aaggtctgcc ttgactacaa gtgatttcgt caactttgcc gaggattttg tagaggagcc
     6901 cgaaaagaag atattacagg tgttcgatgc tgtaagcaaa tttcaatcgt tatgcgccac
     6961 gtatggcata gataaggcta tatttgataa aatccaacat gtattggatg gattggtcac
     7021 tgatgatttg gatttgctgc aattaactta aagaacagta gatggtgata gttgctccag
     7081 tcttttctgg aacggctttc ttttcttttg agatctttat acccctattt gttattttgg
     7141 agaaagtcac atgtttccca cccacccttt cagcggagtg actgtccccg tttcaggatt
     7201 tctcatagtc actcgcaggc tccgtgattg tgtgaaatcc aggaatgggg aaggcaaagg
     7261 agagaggcat atccggtctg ctttcggagc ttcggccaca tcgagacaac ctctttccgg
     7321 cggctatgcc actctccttt tatttgcccg gcagtctgtt gcttacccat atgccccagc
     7381 cttttagggc tgtatatagg tagtcggata tttcgccgct aaatcggcag tccgcttcaa
     7441 tcgtttgctt gcttttctcc gagtggatgc ctttctgttt ttccggttgt ataatcttct
     7501 ttttccggaa acagttgtag tggctgtatg cctgtatggt tatgttctcc ccgcagaatc
     7561 cgcaagtctg ttcttccgtt gcagcctttc attttatcgt gcagtcactt ttttcgatac
     7621 gtggaatatt gtatgcttca ttcttcggct tcattttttt aaaaggaatt gcagaggttt
     7681 cttccggtgt gtcgataggc ggctgaccat attttcggaa tgtgccatgc ttgctgtctg
     7741 accggttgtt ttttgcatcc tttccaaacc tgttgctgat tttcaagttt tgtctttttc
     7801 atatcatttt ttagggaagt caaatcattt tccgttgcaa atatagattg ttgctaaccc
     7861 cttttacggg gataagggga cgattcacaa acaccacgtt gttattcgtc tgaaatcttc
     7921 ctttttttgc cttgcataaa aagcgtattt aacccgaatt atttgctgcc atcttcacca
     7981 ataaaaaagt tacaaatctt cctttttccg gccagcataa aaagagtatt tatatctttt
     8041 ttcatggtgt cccccaaagc caacaatcag ttattgcact gtaaaattga ttaattaact
     8101 tctaaaaaat cagagatatg aaaaagattg aaaattcatt cgcagtaaca ggttttatcg
     8161 gtaaggatgc agaaatccgt agttttgaaa cagcaagcgt agcacgcttt tctatcgcgg
     8221 tcagccgtgc agacaagaca ggtgaagaaa cctcttatgt ttccgctttc atggggattg
     8281 aagcctggag aaagaatgaa gcactggatt ccttcgatgt attgaaaaaa ggtgagctga
     8341 taaccgttga aggctacttt aagccggaag aatggacgga ttctaaaagt ggggagaagc
     8401 gtaaccgcat tgtgatggta gccaccaagt tttatccggc tccggaaaaa gaagaagaaa
     8461 aaccggatca gcctaaacag aaggctgtct ctaaaaagaa aaaagcaagc aaatgattga
     8521 agcggcctag tgccgctttt ttttgctaca tatccgagag tgtttttgcg ttcacgcagt
     8581 attcttcccc accctcccct tcatcggaat gactgttctc attcccggtt tg
//
