LOCUS       NZ_OX460320             1783 bp    DNA     circular CON 15-NOV-2023
DEFINITION  Escherichia coli strain EC0880B isolate EC0880B_R3 plasmid 4,
            complete sequence.
ACCESSION   NZ_OX460320
VERSION     NZ_OX460320.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA112856595
            Sequence Read Archive: ERR11181264, ERR11181596
            Assembly: GCF_949786295.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Wan,Y. and Wan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2023) IMPERIAL COLLEGE LONDON, Shiranee Lab,
            Department of Infectious Disease, 7N12 Commonwealth Building,
            Hammersmith Campus, Imperial College London, London W12 0NN England,
            United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 14:49:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,749
            Genes (coding)                    :: 4,546
            CDSs (with protein)               :: 4,546
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 203
            CDSs (without protein)            :: 203
            Pseudo Genes (ambiguous residues) :: 0 of 203
            Pseudo Genes (frameshifted)       :: 83 of 203
            Pseudo Genes (incomplete)         :: 126 of 203
            Pseudo Genes (internal stop)      :: 52 of 203
            Pseudo Genes (multiple problems)  :: 52 of 203
            Pseudo Genes (short protein)      :: 2 of 203
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX460320.1.
            Complete genome assembly of E. coli isolate EC0880B_R3.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC0880B"
                     /isolate="EC0880B_R3"
                     /isolation_source="Derived from parental isolate EC0880B in
                     laboratory."
                     /db_xref="taxon:562"
                     /plasmid="4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2021"
     gene            join(1514..1783,1..720)
                     /locus_tag="QGS87_RS00865"
     CDS             join(1514..1783,1..720)
                     /locus_tag="QGS87_RS00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001529700.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEILNRMNAAFQRLKDRKEFRPVQGWIRTTEVTRSS
                     DGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(447..638)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 atgtggcaag cccgtttcta ccagtccctg ccgcgtatcg tggcggatta ccctgatgcc
       61 cgctggatgt tcctgaccct gaccgtgcgc aactgtgcga tcggggagct gggcgagata
      121 ctgaaccgga tgaatgcagc tttccagcga ctgaaggatc gcaaggagtt ccggcctgtg
      181 cagggctgga tacgtaccac ggaagtcacc cgcagcagtg acggcagcgc acatccccac
      241 ttccacaccc tgatgatggt gccgccctca atgctatctg gtgacggtta cgtaaaacat
      301 gctcgctggg ttgaactctg gcgggagtgc ctgcgggtca gttacgaccc gaatgttgat
      361 gtccgggcag taaaaccccg gaagccgaag gacggcgaat cgctggcctg cgcgaccgcc
      421 gaactggtgc gcggtgcggt ggccgaaacc ctgaagtatt ccacgaagcc ggctgacatg
      481 gtggccgatc ctgaatggtt cctcgaactg acccgccaga cgcacaagcg ccgctttgtg
      541 gcgactggcg gcgcactcaa agacgtactg aagctcgatc aggaaaccga cgcagacatg
      601 gtgatcgggg atgatatttc cgagggcgac gatgacggtt cccgcatcgc gttcgagtgg
      661 aaaactgagg tcagaaagta ccgtcgctca ccgacgaaag ataaaaccga atcagagtaa
      721 cggggtgtcg gggctgcgcc ctgaccatga ggactttggg catccgtcac cttggtggcg
      781 gtgtaccaaa gtcacatctt gtccgattgg ttttactccg atgcaggata gggctaacgc
      841 cgcaaagtgc tagcactcga ttgcctagtt cggaatccga acttagggtt ttgcgatccg
      901 taatacggat tttttttgac gggacaagat gtgtttttgg agtaccgcca tcacgatgcg
      961 ggtaccaaaa acgatccgtc agggctgcgc cccgacaccc tgccaacacc tcacgccgcc
     1021 agattgaaat ctgccagcgt tcgccgttcc tgaaatcgtc aaacgctaaa tcgtggggtc
     1081 ccgctggctt gccagtgggt tccgatttag tgattgacga ccgcgcccgt aaaacactgc
     1141 cgacaggtgg ttgtgatcac gcagtgagcg caaccacctg gtaagcggtt ttgggttttt
     1201 ttgggggtga tttaacgcgg ttttcgggtg gcaaatctcg cgcgcgatcc ttgtatttat
     1261 acttaaggga taactctggg attcaaattg gcctccagcc cttgtcccgc aaggcttgag
     1321 ggcacatttg ctccgaaatg ggtttagaag tattctgtcg gaagtggtcg cgttaaaagc
     1381 gacccccgca acgacgggca tcgttcgggg gcgtgaccac ctgtagagat tcgaattggt
     1441 gatacggcta acacaggtga tatggcaatg ataacacgtc agatgagcca tcgcccacaa
     1501 tcgggtggtg tgcatgaaaa atcctgaact ccagaacctc acggactatt ccccctcaga
     1561 tgcgccctgg gacgctcaca gaagcgtttc tgacgacgtg ggagggattt acctgcttgc
     1621 ggccgaatat gagcgctacg gggcgcgtat ggccctctgt ggcggtttat tgcgtttcgg
     1681 gtggtctacc ctcaaggaaa ccggagaaac gcgtctgagg ctgcgtgagg cgcatttctg
     1741 ccgtgtccgg cactgtcctg tctgccagtg gaggcgctcc ctg
//
