LOCUS       NZ_OX359177             5875 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP47 isolate 2018-047100-007-01 plasmid
            p02, complete sequence.
ACCESSION   NZ_OX359177
VERSION     NZ_OX359177.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA111391101
            Assembly: GCF_947163785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Pongolini,S., Scaltriti,E. and Menozzi,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2022) IZSLER, Risk Analysis and Genomic
            Epidemiology Unit, IZSLER, Risk Analysis and Genomic Epidemiology
            Unit, Strada Mercati 13/A, 43126, Parma, Italy, Italy
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 02:24:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,439
            CDSs (total)                      :: 5,315
            Genes (coding)                    :: 5,169
            CDSs (with protein)               :: 5,169
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 146
            CDSs (without protein)            :: 146
            Pseudo Genes (ambiguous residues) :: 0 of 146
            Pseudo Genes (frameshifted)       :: 75 of 146
            Pseudo Genes (incomplete)         :: 81 of 146
            Pseudo Genes (internal stop)      :: 23 of 146
            Pseudo Genes (multiple problems)  :: 30 of 146
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX359177.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP47"
                     /isolate="2018-047100-007-01"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p02"
                     /geo_loc_name="Italy"
                     /collection_date="2018-02-07"
                     /collected_by="Policlinico SantOrsola in Bologna"
     gene            complement(1067..1258)
                     /locus_tag="ONI05_RS27085"
                     /old_locus_tag="KPNEU47_KP47_05252"
     CDS             complement(1067..1258)
                     /locus_tag="ONI05_RS27085"
                     /old_locus_tag="KPNEU47_KP47_05252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021539356.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1324..1647
                     /locus_tag="ONI05_RS27090"
                     /old_locus_tag="KPNEU47_KP47_05253"
     CDS             1324..1647
                     /locus_tag="ONI05_RS27090"
                     /old_locus_tag="KPNEU47_KP47_05253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024188420.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAAWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALIAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1856..>2287
                     /locus_tag="ONI05_RS27095"
                     /pseudo=""
     CDS             1856..>2287
                     /locus_tag="ONI05_RS27095"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2325..2855
                     /locus_tag="ONI05_RS27100"
                     /old_locus_tag="KPNEU47_KP47_05254"
     CDS             2325..2855
                     /locus_tag="ONI05_RS27100"
                     /old_locus_tag="KPNEU47_KP47_05254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            2862..3095
                     /locus_tag="ONI05_RS27105"
                     /old_locus_tag="KPNEU47_KP47_05255"
     CDS             2862..3095
                     /locus_tag="ONI05_RS27105"
                     /old_locus_tag="KPNEU47_KP47_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021520366.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3815..4867
                     /locus_tag="ONI05_RS27110"
                     /old_locus_tag="KPNEU47_KP47_05257"
     CDS             3815..4867
                     /locus_tag="ONI05_RS27110"
                     /old_locus_tag="KPNEU47_KP47_05257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021517772.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGNGGYSHPEGISTTWHSESKLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIDNSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGVPVDDASMFFNSFQPRKFE
                     NNPLYEKEDD"
                     /name="hypothetical protein"
     gene            4869..5735
                     /locus_tag="ONI05_RS27115"
                     /old_locus_tag="KPNEU47_KP47_05258"
     CDS             4869..5735
                     /locus_tag="ONI05_RS27115"
                     /old_locus_tag="KPNEU47_KP47_05258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     Typing          892..973
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          248..435
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          1638..3199
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.232"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agacgtttgt aacaaaaaga tcaaaggatc ctcttgagat cctttttttc tgcgcgtaat
       61 ctgctgcctg taaacgaaaa aaccgccttg cagggcggtt tgatcgaagg ttaagtcagc
      121 ttgcagactg cttaaccagg taactgtctt atgcgagacg agtcaccaaa attgttcttc
      181 tagtgtagcc gcactccggc agccacttca agtactctct aaatatctcg cacatgactt
      241 ttaccaatgg ctgctgccag tggcgctttg tggtgtcttt tccgggttgg acccaagaga
      301 acagttaccg gagaaggcgc agcagtcggg ctgaacgggg ggttcctgca tacagcccag
      361 cccggagcga actgcctaag tcgccccgag ataccagaag acgtgagcta tgagaaagcg
      421 ccacgtgtcc cgtaagggag aaaggcggaa aaggtatccg gtaatcgata tggcgggaac
      481 gtgggggcgc aggagggagc gattcaccgg aaacggtgat gatctttaag ccacgtcggg
      541 tttcgccacc gctggcggat ttcatggttg agccgcaagg ctcccacaga tgcaccgaaa
      601 aagtgtctgt tttgtgaagt ccgtcagggg ggcggagcct atggaaaaag cgcctccggc
      661 gcggcctttt ccggggtttc gttttttgcg cacatgttct ttcctggttt atccccgggt
      721 tctgtggata acagggttag cgtttttgag aaaggagagt ccgctcgccg cagccgaacg
      781 accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gcctgaactg gcattttctc
      841 cttacgcatc tgtgcggcat ttcacacccg acatggcgca cttttcatac aatccgcact
      901 gatgccgaat gattaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt
      961 cgccccgaca ccccaaaggt caacggcctg acgcgccctg gcgggcttgt ctgctcccgg
     1021 catccgctca cagacaagct gtgaccgtct ccgggagctg catgtgtcag cggttttcac
     1081 cgtcatcccc gaagcgtgcg aggcaactgc ggtaaagctc atcggcgtgg tcgtgaagcg
     1141 attcacagat gtctgcctgt tcatccgcgt ccagttcgtt gagcttctcc agcagcgtta
     1201 atgtctggct tctgataaag cggaccatgt taagggcggt tttttcctgt ttagtcactg
     1261 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgcgag
     1321 aggatgctca cgatacgggt gactgatgat gagcatgccc gtttactgga acgctgtgag
     1381 ggtaaacaac tggcggcatg gatgcggcgg gtctgcctgg gtgagcctgt tgcccgttcc
     1441 gggaagctgc cgacactggc accgccgtta ctgcgtcagc tggccgctat cgggaataac
     1501 cttaaccaga cagcgaggaa ggtgaacagc gggcagtggt cttccggtga ccgggttcag
     1561 gtggtggccg cactgatagc catcggggat gagctgcgcc ggctgcgtct ggcggtcagg
     1621 gaacaggggg cgcgggatga tagttaagtt tcatgccagg ggaaaaggtg gtggcagtgg
     1681 tccggttgat tacctgctgg gaagggagcg taaccgggaa ggggcaacgg tgcttcaggg
     1741 gaatccggaa gaggtccggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc
     1801 aggtgttctg tcgttcgcgg agaaggagct gccgcctgga gggcgtgaaa aagtgatggc
     1861 gagctttgag cgtgtactga tgcccggtct cgagaaaaat cagtacagca tcctgtgggt
     1921 ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca
     1981 gaccggaaaa cgcctccagc cgtactacga ccgggcagac aggccgagaa ttgatgcctg
     2041 gcagacgctg gtgaatcacc attacggtct gcatgacccc aacgccccgg agaaccgccg
     2101 gacgctgacc cttcctgata acctgccgga aacgaaacag gcgcttgctg agggcgtcac
     2161 acgcgggata gacgcgcttt accatgccgg agagataaaa ggccgtcagg atgtgattca
     2221 ggcgctcaca gaggcggggc ttgaagtggt cagggtgacg cgaagcagta tcagcattgc
     2281 agacccgaac ggcgggagga atatcagact gaaaggagcg ttttatgagc aatcttttac
     2341 agacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc
     2401 tgaacgacga gttcaggaag ctcgaagaat ctgtaaacag ggaactgacc tcaaacgaat
     2461 ctttaatcag gaacgctatc aacgaccata caacagccct gaaagaactg ctggagaggt
     2521 atcagaaaac gaccgtcgat acgatggacg cgcactggaa aaccgtactg aaaatgagcg
     2581 tgaaacgctg gctgtggctg attatcgtct cagtactgat gtttgccacg accgggagtc
     2641 tgctgtggta ccagggcatg aagataaacg cgaatatgaa tattctccgc gagcagaaag
     2701 agagtctgga gaagctgaac gcgaagacat ggggcgtgcg ttatcacgag gacagcaacg
     2761 gacgttttct ggtgttgccg aaggggatga aagccgaaac gaactggacg aaggacaacg
     2821 gcaaactgaa cgcagtgaga ctggtgcagg agtgacagag catgacggag ctggaaagac
     2881 atttgctgaa cgcattagag caactgcaac aggactatat gcagcggctg aacgaatggg
     2941 agaacgcctt cgtggaattg cagaaaatgt tttcgcttac gcaacgggac aacgcgatgc
     3001 tgaacgggcg agtcatgcag ttgagtcagc aggtgcagca cttgagcgag cggacagaac
     3061 gcttgagcca gttatacagc gagaactgga gataagggat gagcggctga tacaggagcg
     3121 cgaatatgcg ttatccctgg agcgggagcg ccagccggaa attcaggaac ggacactgga
     3181 tggcccttcg ctgggatggt aaccagagac aggctaaaat gttggcagcg cgtttccggt
     3241 gcattttttg ggataatggt tggttcgggg gcaccgggaa cgcgcttcag tcagagcttg
     3301 ctgaaaacac aggaacgcgt taaaacgcat gcgggcgcga ttctggtgtg tctttgttgc
     3361 tgtgttttgc cgttattgcc agatcgcgct cattcccatg aatttctgtt tttccgctcc
     3421 ggttccgtct tctgcctttc cgaaagtatg aaaaaaatcc gtttcagcag tctctggctg
     3481 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttatcgggt
     3541 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3601 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     3661 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt taaaatatcg
     3721 gtaacgataa taaattattt cgttttacgt cggtaataaa tataatgtag tttcagtata
     3781 taaaaaatat atgaggtgtc ttgtgggagc tttcatgaat cttcaacaac gtataaataa
     3841 actaccacaa ttatcatcca gtttttcatt tggaaaagat attgataata ttcattcatt
     3901 tatctttaac gaaacttcta aagataaaat tgaagattta ctaagaaaat gggtgtcagg
     3961 caatcagccc tgtgtatttg gtaaattggc aagcaagaaa attaaagggc ttgatttcca
     4021 tttatctatt gtaaatagcc cccaactata taatgatgat ggtcatttgt ttgatttttt
     4081 gcgtaatgaa agagttcgtt ttaaagaacg cgcaagaaga ggagaggtca gtgcgcatct
     4141 catttatttt attcatccac aacttgcatt tgctcgtcca tcagaagagt tagtggacat
     4201 acaaaaatat atttgttcgc tgcatatgcc agagtgctat ccaataaaag aggatgttat
     4261 ttatactgaa agcgtaccat ttcaggataa agatgggcta aaaatatata aggctggtgt
     4321 taatgttttt tactcttctg ctcatagaac aagaaatcat gatagaagaa taccgggggg
     4381 aatacttatt tctgtaaatg ccccggggca ttttatgagg ctggcgatag aaaaaggttt
     4441 ctataaagat caagaacaag cgttagcgga tattcgtaac atgactattc agtctgttgg
     4501 taacggtggg tattcacatc cggaagggat ttctacaaca tggcattcag aatctaaatt
     4561 ggatcgtttt ggatgcccag tgcacaccgg taatagttct tattacagcg gtttttatca
     4621 tactgatgta ttaattccag gagaattaac taaggatgaa agattgctcc atgaaataga
     4681 taatagtgat ccaatgatct tcaactggaa tgtattgttt tatgtttcat tagaagagtt
     4741 tcctatagat gatccttatt atggagagtt tattggtgtt cctgtagatg atgcatctat
     4801 gttttttaac tcctttcaac caaggaaatt tgaaaacaac cccctttatg agaaagagga
     4861 tgattaatat gattaaagga atgctttttg cttttttagc agcgttctca tggggggcag
     4921 ctatcgtaat gagcaaaaaa ggagttgaag aactagatgc cggaggggtg tttttctggc
     4981 agatttgtag tgcagtaata ctatcttgga tagttctttt tataaaaaga aaaaaactcc
     5041 cattaaactc aaaagcgata cttgcttatg cgacaggcgt ttttgaacca ttcttagcgt
     5101 atacttttac actctacgga ctttatttta tttcagcagg aagcgcatca attatttttt
     5161 cacttgaatc agtgtttata ttggtgttgt ctgttcttat ttgctcagtt aaaattaatt
     5221 ccccgcaata ttttatattg ttccttatag gtgcaattgt tggcagtatt ttggtagcct
     5281 tgccagacat cagtaatgga tcgaataata ttgttggata ttgtcttgtt gttgccggtg
     5341 tgttatcggc agcattttat gttgttatat cgtctcgcct tattaaatcg tttgagccta
     5401 tcacattatt gagcggacag ttgacattct cccttatact gtcatcgctc tttattttta
     5461 tatctaaatc ctcaatttat cttccgttgg aatccgtgat tattgttttt tcatctggaa
     5521 tattacaata ttttttagct ttttgttttt acttgaactc cttgcgttgg ctcccagttc
     5581 atttggcagg gataatgttg tactttattc cgctagttgc attatttttg tcatggttgt
     5641 ttttggatga acaattcaca ttcttacagg gggtaggatt tattgtaact gtgctaagtg
     5701 tatattttct taacagaaaa tactcagagg agtaacattc tgttcaggat tgcccccccc
     5761 ccggcctcca tcgataccgt gccaaatgca cgtaaaaaac gggagcgttt ctggtgtcgg
     5821 aatgtaacca aggggggcag aggcgcgcta aaacgctctc aggggagtca aatag
//
