LOCUS       NZ_OX291799             3223 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1050 plasmid 4, complete sequence.
ACCESSION   NZ_OX291799
VERSION     NZ_OX291799.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201017
            Assembly: GCF_946415285.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:45:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,246
            CDSs (total)                      :: 5,115
            Genes (coding)                    :: 5,043
            CDSs (with protein)               :: 5,043
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 44 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 11 of 73
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291799.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1050"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="4"
     gene            join(3120..3223,1..229)
                     /locus_tag="N4J75_RS26210"
     CDS             join(3120..3223,1..229)
                     /locus_tag="N4J75_RS26210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            1247..1570
                     /locus_tag="N4J75_RS26215"
                     /old_locus_tag="SAMEA13201017_05199"
     CDS             1247..1570
                     /locus_tag="N4J75_RS26215"
                     /old_locus_tag="SAMEA13201017_05199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1779..>2213
                     /locus_tag="N4J75_RS26220"
                     /pseudo=""
     CDS             1779..>2213
                     /locus_tag="N4J75_RS26220"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2248..2733
                     /locus_tag="N4J75_RS26225"
                     /old_locus_tag="SAMEA13201017_05200"
     CDS             2248..2733
                     /locus_tag="N4J75_RS26225"
                     /old_locus_tag="SAMEA13201017_05200"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2737..2952
                     /locus_tag="N4J75_RS26230"
                     /old_locus_tag="SAMEA13201017_05201"
     CDS             2737..2952
                     /locus_tag="N4J75_RS26230"
                     /old_locus_tag="SAMEA13201017_05201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          960..1151
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          complement(497..977)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          1561..3050
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactgcaata agttattgcg ctgcctttta tctgctttcc caaacattgg aaaatatacc
       61 taccggtatt gcttacgcaa tttggtctgg tgcgggaatt gtactaataa gcctagctgg
      121 ttggttggtt agcggtcaaa aacttgattg gcccgcaatt gcaggaatgt tccttatttg
      181 cgttggagtt ttggttatta atattttttc aaaatcttct gcgcattaac tttttaccga
      241 agcatattgg ccaaagcaaa aggatttcct tgagatcctt tctttctgcg cgtaatcttt
      301 ttccctgtaa acaaaaaaac caccgctacc aacggtggtt cgtttgccgg aattcagagc
      361 taccaactct ttgttccgat ggtaactggc tttcacggag cgcagatacc taaactgtcc
      421 tttcagtgta gccgcactca ggctttcact tcaagaactc tgtaggcatt ctctacaatc
      481 tctgctccgt gaacccagtt accaatggct accgccactg gcgatttgtc gtgtctttcc
      541 gggttggact caagttgata gttaccggaa cgggcgtggt agtcggactg aacggggggt
      601 tcgtgcatac agtccatcct ggagcgaact gcctacccgg aaccgagttt caggcgtgga
      661 atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaatgcgg
      721 gggagcacga gggagccacc agggggaaac gcctggtatc tttaagccgc atcgggtttc
      781 gccaccgctg atttgagcgt cattttcgtg atactcgtca ggggggcgga gcctatggaa
      841 aaacggcaac tccgcatcct tttcttggcc tttcctgctt cgagcggcat ttgcttatcc
      901 cgtaatctgt gagctggtaa cgcccgccgc agtctcacgg caggagcgta gcgaccgagt
      961 gagcgaggag gcgtcatttc atcaccacct gcattttgca cttacgcccg gcagccgcat
     1021 ttttcccctg tcacatgaag cactttcata aattaccact ccgtcataac atgtttagcc
     1081 agtatacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa acccgctaaa
     1141 accactgccg cgtctgcggc ttgtccaaca ccgtgccgac cgggaaaaga tttcccgtcc
     1201 gtcccggcgt tatcaggagg ccggattggc agacaaacgc agcaaaatgc tcaccatgtg
     1261 ggtgactgag gacgagcacc ggcgtctgct ggagcgctgc gagggtaagc aactcgcggc
     1321 ctggatgcgg cagacgtgcc tggacgagaa gcccgcacgc gccggcaaac ttccgtcgat
     1381 ctcgccggcg ctgcttcgtc agcttgccgg catggggaac aaccttaacc agattgcccg
     1441 ccaggttaac gccggtggtg gaagcggaca cgaccgcgtg cagattgtcg ccgcgctgat
     1501 ggccatcgat gccggactcg agcggttgcg gcatgccgta ctggaaaagg gtgctgatga
     1561 tgatcgttaa gtttcatcct cgggggcgcg gcggcggcgg cgggccggtc gattatctgc
     1621 tgggtaaaga tcgccagcgc gacggcgcca gcgttctgca ggggaagccg gatgaagtcc
     1681 gggagcttat cgacgcctcg ccctacgcca aaaagtacac ttccggcgtt ctgtcttttg
     1741 ccgaacagga tttaccgccc ggccagcgcg aaaagctgat ggcgagtttc gagcgggttc
     1801 tgatgcccgg actcgataaa gaccagtaca gcgtgctgtg ggttgagcac cgggacaagg
     1861 ggcggctgga gctgaacttc ctgatcccga acacggagct tctgaccggc aagcggctac
     1921 agccgtatta cgaccgcgcc gaccgtccgc gcatcgatgc ctggcagacc atagtgaacg
     1981 gcaggctggg gctgcatgac ccgaacgcgc cggagaaccg gcgggtgctg gtctcgcctt
     2041 ccgcgctgcc ggaagcgaag caggaagccg cccaggcgat tacgagcggc ctgcttgccc
     2101 ttgcctcatc cggggagctg aaaacgcgtc aggacgtcac tgaggcgctg gaaagcgcag
     2161 gttttgaggt ggtgcgcacc acaaaaagca gcatcagcat tgccgacccg gacggggggc
     2221 gaaacatccg actgaaggga gccatctatg aacagtcttt taacgctggc gaaggactta
     2281 gagcagaaat cgaaagcgca gcagcagact accggcgaga tgctgaaagc cgcattcagc
     2341 gagcacgaga agtctgtcag agcggaactg agcgaaagcg agaagagaat cagcgccgcc
     2401 atcctcgacc acgaccggaa gctgtcctca gccatgagcc agcgcacgaa agggatgctg
     2461 cgcatggtca gccagacgtg gctgaccatc gttctggtct ccgcgctgct gatagcgtcg
     2521 agcgcgggca ttctgtggtg gcaggggcag cagatactcg agaactatac gaccatccgg
     2581 gagcagaaga gcacgcaggc catgctgtca gagaggaaca gcggcgtaca gctctcgacc
     2641 tgcggcgagc agagacgccg ctgcgtgagg gtgaacccgg aagcgggaca gttcggagag
     2701 gactcgagct ggatgatact ggcggggaaa tagcacatga cggagctgga aaaacagttg
     2761 ctgagcgcat tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagaac
     2821 gccttcgcgg aatggcggac gatgtctggt cttattcaac gggagaacgc ggcgctgaac
     2881 gagcgcgtca cggtcttgag caggcaggtg cagagtttga gcgagcagct gcgccggttg
     2941 tcgaaaggct gaacgcgata gaaactcacc ggcagcagga gcgggcggtg cagcaccaga
     3001 aagcgctgga gctggaacga tcgcagtggc agcatcatgg tccttcgcta tgataagccg
     3061 ggttctatta attagttgtt atgtaaagat atttaaattt ttcaacatga ggtattttta
     3121 tgaatacata tattttcctg ggaggggcta ttgtggctga agtcataggc accaccttta
     3181 tgaaatattc agaaggattt acacgcctgt ggccgtctgt agg
//
