LOCUS       NZ_OX291772            12879 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1044 plasmid 2, complete sequence.
ACCESSION   NZ_OX291772
VERSION     NZ_OX291772.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201013
            Assembly: GCF_946414065.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:43:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,244
            CDSs (total)                      :: 5,113
            Genes (coding)                    :: 5,041
            CDSs (with protein)               :: 5,041
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 28 of 73
            Pseudo Genes (incomplete)         :: 44 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 11 of 73
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291772.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1044"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="2"
     gene            join(12796..12879,1..501)
                     /locus_tag="N4J73_RS26100"
     CDS             join(12796..12879,1..501)
                     /locus_tag="N4J73_RS26100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351424.1"
                     /translation="MDITISDATYSRLAAQAKGFETPEQVINRLIDVVEIDHDTRPELF
                     FNPEGEADFKKALLKSKQAKVEMFKRDGSCETGVWNARAISEESSVRANIWSGYLRGWK
                     EKGIVRAVFTVQEVPDLPGAAESVQWNDYTISRTFSGGISVQRNGMPVTIVKPFLRDVA
                     HDLKISLLNSNGNEMNTRQLGAAIIKKLNNS"
                     /name="hypothetical protein"
     gene            complement(756..1214)
                     /locus_tag="N4J73_RS26105"
                     /old_locus_tag="SAMEA13201013_05182"
     CDS             complement(756..1214)
                     /locus_tag="N4J73_RS26105"
                     /old_locus_tag="SAMEA13201013_05182"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765952.1"
                     /translation="MKSKTELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNTLATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDGAAA"
                     /name="hypothetical protein"
     gene            complement(1440..2621)
                     /locus_tag="N4J73_RS26110"
                     /old_locus_tag="SAMEA13201013_05183"
     CDS             complement(1440..2621)
                     /locus_tag="N4J73_RS26110"
                     /old_locus_tag="SAMEA13201013_05183"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765953.1"
                     /translation="MAFDNSHLTDAGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSTRHNRGSAHHRSQYRHFLKLKGVTNPEAMAQVFLRFSGEVYWRLDSNR
                     TPPSTEQVAALAERKRRSIEAVARREDADRRKADKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDTSPHAPYSPLFVCEGFATAVALH
                     HLTKGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPV
                     PTIPGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            complement(2608..2817)
                     /locus_tag="N4J73_RS26115"
     CDS             complement(2608..2817)
                     /locus_tag="N4J73_RS26115"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765954.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPVLKRFIADQRRPGRAPYGVR"
                     /name="hypothetical protein"
     gene            3173..4462
                     /locus_tag="N4J73_RS26120"
                     /old_locus_tag="SAMEA13201013_05184"
     CDS             3173..4462
                     /locus_tag="N4J73_RS26120"
                     /old_locus_tag="SAMEA13201013_05184"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157698538.1"
                     /translation="MNDDNDTTEATQPSTEALKCVGAAERFPDIMPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQGEQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRNRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            4459..5100
                     /locus_tag="N4J73_RS26125"
                     /old_locus_tag="SAMEA13201013_05185"
     CDS             4459..5100
                     /locus_tag="N4J73_RS26125"
                     /old_locus_tag="SAMEA13201013_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_070559060.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELNMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(5258..5614)
                     /locus_tag="N4J73_RS26130"
     CDS             complement(5258..5614)
                     /locus_tag="N4J73_RS26130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559062.1"
                     /translation="MPRHLQGKTRMPLAGGCYSAGHRLALISLLPVLEARPGDRDDGER
                     SKLALYQESLIKAGQPHAFALHEPSLYRWGAYLKSRRARYLQETDAGVAAASDALDRHA
                     FGRAIGAVLGADDE"
                     /name="hypothetical protein"
     gene            complement(5620..6003)
                     /locus_tag="N4J73_RS26135"
                     /old_locus_tag="SAMEA13201013_05186"
     CDS             complement(5620..6003)
                     /locus_tag="N4J73_RS26135"
                     /old_locus_tag="SAMEA13201013_05186"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559064.1"
                     /translation="MSAFSMLCDRLRHEATLAKSTGAGDALAQVLETGGAAQTFAPIGA
                     PVASPTESVHRARAQSIQDAAFLTEMKQAGVAPADLVMLDGVLASSRGLPDASRAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            complement(6004..6660)
                     /locus_tag="N4J73_RS26140"
                     /old_locus_tag="SAMEA13201013_05187"
     CDS             complement(6004..6660)
                     /locus_tag="N4J73_RS26140"
                     /old_locus_tag="SAMEA13201013_05187"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879883.1"
                     /translation="MTEIKTECPDATWLRTTLAEMASDHFPVYDLPCLSVDPNSSAQLT
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLRTEAERLRGLIADGKTALRAGETVSPD
                     AGVAAFLLPDIEAELVAAEAAEADVARERDALLQDADRRDAAAAIGLFNLAHAVRVQRI
                     EQLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEA"
                     /name="hypothetical protein"
     gene            6920..7507
                     /locus_tag="N4J73_RS26145"
                     /old_locus_tag="SAMEA13201013_05188"
     CDS             6920..7507
                     /locus_tag="N4J73_RS26145"
                     /old_locus_tag="SAMEA13201013_05188"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181654175.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQILQEGDTLVIYAIDRIGRNARDALELVEELEKRKVTLVSIREGVDLS
                     SAVGQAVLGIMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            7618..7803
                     /locus_tag="N4J73_RS26150"
                     /old_locus_tag="SAMEA13201013_05189"
     CDS             7618..7803
                     /locus_tag="N4J73_RS26150"
                     /old_locus_tag="SAMEA13201013_05189"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879885.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDSA
                     GRYQTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            7922..8167
                     /locus_tag="N4J73_RS26155"
                     /old_locus_tag="SAMEA13201013_05190"
     CDS             7922..8167
                     /locus_tag="N4J73_RS26155"
                     /old_locus_tag="SAMEA13201013_05190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558916.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTVANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            8366..8833
                     /locus_tag="N4J73_RS26160"
                     /old_locus_tag="SAMEA13201013_05191"
     CDS             8366..8833
                     /locus_tag="N4J73_RS26160"
                     /old_locus_tag="SAMEA13201013_05191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_070559148.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATAATVARTMGIPPRTVRDWAKART
                     DAPSNVLSIEEMRERAQRAVEATPQAAIRRLKNHFVQRQFELLQRHATDLQALRTASLQ
                     AMLEKDATMVKAISGLMTSLLKTQERERVIYEIKPGTEADIMREGMNRQQS"
                     /name="transposase"
     gene            complement(9479..10774)
                     /locus_tag="N4J73_RS26165"
                     /old_locus_tag="SAMEA13201013_05192"
     CDS             complement(9479..10774)
                     /locus_tag="N4J73_RS26165"
                     /old_locus_tag="SAMEA13201013_05192"
                     /inference="COORDINATES: protein motif:HMM:NF017116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_119879886.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKLDVDDCDPTAFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            11302..11793
                     /locus_tag="N4J73_RS26170"
     CDS             11302..11793
                     /locus_tag="N4J73_RS26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558888.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGT
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQTAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            11858..12349
                     /locus_tag="N4J73_RS26175"
     CDS             11858..12349
                     /locus_tag="N4J73_RS26175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
ORIGIN
        1 cgtctgatcg atgttgttga aatcgatcat gacacacgcc ctgaactttt ttttaatcct
       61 gaaggtgaag cggattttaa aaaagcgtta ctaaaatcaa aacaggccaa agtcgaaatg
      121 tttaaaaggg atggttcctg tgaaactgga gtatggaatg ctcgtgcgat ttcagaagaa
      181 tcatcagttc gggcaaatat ttggagtggg tatttgcgtg gatggaagga aaaagggatt
      241 gttcgtgcgg ttttcactgt ccaggaggtt cctgacttgc caggagcggc tgaaagtgtg
      301 cagtggaatg attatacaat cagtcgcact tttagtggcg gtatttcagt tcaaagaaat
      361 ggcatgcctg taactattgt gaagccattt ctacgtgatg ttgcgcatga tttaaaaata
      421 tcattgctaa atagcaatgg aaatgaaatg aatactcggc agcttggcgc agcgatcata
      481 aagaagttaa ataactctta acatcgatgc ttcacgcaga ggagcgacgg ctcaacgccg
      541 ccgccggtca gagttctact gcgacgaata cgcaccattc atagctggcg aggcagaacg
      601 cccgccacct gtcatcagac gcccggtcaa cacactccag ccataaccgc gccgcagcaa
      661 ggtggtcgca gcgcaccggg tcgccccgca gttccgtcgc catggcctcc tcgatcagtc
      721 gcccttccca ctccagctga tcgaggatcg agcccctacg ccgccgcccc atcgagccac
      781 tgcagcatcg ccgccctgat ctgccccgcc tcgctctcgt ccagcgtcag gaacgccaga
      841 tcggcaacct tacccagccc ggcgaacgcc acgcagccgg tgacggagaa cagctccacg
      901 aggagcgccg tcgccagcgt ctcccgttca acgccgcaca accaggcgta gttacgctca
      961 cgcgatgccg gaagtttcac ctgcagcgca cgggtggcca gagtgttaaa tttctcggta
     1021 ccagcccact cgtagtcgaa cggcgggcga tcaaccacca ccgacccgga cagcgtctgc
     1081 tggcgttccc cagcggagaa gttcggcagc ttgaggtggg agaacccatc gagcgaccac
     1141 tccgacattt cagtggcgat caccgccaga gagcgagccg tcgcggcctc gcgtagttca
     1201 gtctttgatt tcatgtgccc tcctttgggc taaaaaaaaa cccgacatca agacgaggcc
     1261 gggctttatg ctgctgtgag aaaccgatcg caggatagcg tcacgacggc agcgccggtg
     1321 cgtcgcttta gtccgtctca tcaaaaaagc atcggtagtg tctcgaaagt cgttcgctaa
     1381 accagacgcc acgacggcaa aaaagatgtc ccgaaaggcg caccattcgg acacgccaat
     1441 caacgccgct caagccgggc atgacgaacc gcgtagcgca gcagctcacg agcccgatct
     1501 tccccttcct caaccagcac gtcgaaccag tcgaggttat cccggggcgt gaagcgatcg
     1561 aggccgtcga acgacacaac cgccgcgcct ggaattgtcg ggaccggctc tcgctcatcg
     1621 ccataggccg ctttatgcgc caggcggtac gcttcgccgt gatccggtac cagcatcaca
     1681 tcaacatctg gccagaaccg gcgcagcgac gccacgcagc tgccgaaccc ggcaattgag
     1741 aacgccgcgc agactgccac cttgcccttc gtcagatgat gcagggcaac cgccgtggcg
     1801 aacccctcac agacgaacag aggcgagtag ggcgcatgtg gtgaggtatc gagcacgtcc
     1861 ataaagccgg gcatcggata gaaccgtccc gccttttggg tacctgcagg ggagaacttg
     1921 tcgccggttt tgccgccagc agagcagacg ccgcagatgg cctccagcgc gtcgccaacc
     1981 agctggcgat catcggtcag gcgataggca ggaacaagca gacagcgcga ccagtacgcc
     2041 ctcggttcga acgagcggaa caagccaccc acacgaaggt cagcggggag gggcgggagg
     2101 ctgacgccac cacgcctggc gattgtggtc ttattgcgcc agtacggggt ataggccgga
     2161 ttaccgtcgc ctttcgccag catcctgacc gcatcagccg cagccttatc ggccttgcgc
     2221 cggtcggcat cttcgcgtct ggccaccgcc tcgatcgatc ggcgtttccg ttcagcaaga
     2281 gcagcaacct gttcagtcga cggcggcgtg cgatttgagt cgagccgcca gtacacctcg
     2341 cctgagaacc gcaggaatac ttgcgccatg gcctcgggat tggttacgcc tttcagcttc
     2401 aggaagtgcc ggtactgcga ccggtgatgc gcagatccgc gattgtggcg ggtagaccag
     2461 gcctcgatag cagcaagccc gtcctctccg cagagagatg cccagcatgc gatgccagaa
     2521 cagaggcgca gccagtcctc gcggtcaacc ggagccccag ccgcagagac ggcacgccag
     2581 aacaggccag cgtcagtcag gtgagagtta tcgaacgcca taaggagccc tcccgggacg
     2641 acgctggtcg gcaataaagc gtttgagcac gggatcccca gccagccagt aatgcccgcc
     2701 acgaagggaa cgccctgccg gagtcggaac gatcccccgc gaactcaggc gggagagcgt
     2761 gcgcagcgac acgttcagca gcgcagcgag gtcagcggta tagattcgtt ttttcattgg
     2821 gagtaccaga taggtgcccc cctcagctta acgggcatcg gggggcggga gtgacgatta
     2881 tcgataaagc cgcccttgag gacttaccgc atagcggcag gacagcagcc cttctcgctt
     2941 actcgcacct gccaaggacg aagcgcgcat cctcgtttac aaccccgcct cgttggggag
     3001 gagggaaagc ggcaccgggc gatccggttt catgtttccc aactgctcgt caggaatcag
     3061 taagacaagc ttaacacaat taacgccaaa gtggcgtttt ttgtgagata ctaaaaaaga
     3121 cgcacagcca gtgctggcga ggtctggcga ccatttcgac gcagggggag cagtgaacga
     3181 cgataacgac acgacagaag cgacgcagcc gtcaaccgag gcactgaagt gcgtaggcgc
     3241 tgcggagcgg ttccccgaca tcatgcccgg cgttttgttc gtcacctgcc ccggcgggtg
     3301 ctccctccgg gtcgaggcat gccgacagat ttatgagcag agccacctcg atcgcacgat
     3361 tgaaaacccc cacctcgagt gccaccggtg ccccactggc ctcgcgcttt gggtcggtac
     3421 cgaacagccc cctgacgcac cactgaccac cccggcagac acccgtaagc tctgcccccg
     3481 ttgcagccgg aaagacaacc gcctgatcgc ccccaataag cccggttcga gcctgtgcgt
     3541 ctcctgctgg aaccgcgaac gcgaagccac catcggagcc aacgcacgcg ggagagtcaa
     3601 ccgcacccca ccgttcgtct cgccgttcct gatcggcatc ggcgatggct ggcaggtctg
     3661 gtacggcatc caccagggcg aggcgctcgt ccgcgccatg cgagccaacc cgggcgcacg
     3721 gttccacgat cgccgaccag gcccggtcgg gcaaacacgc cgcgacggtt tcgtgtacta
     3781 ctgccccgaa caccgcgagc ccctgcgctg gaacggtggc gacgggcacg ccggagaggt
     3841 ggagcttgct tgtcccaggt gccagccatg ggcggcacct ctcagcgtcg cccccacgtt
     3901 tcagacggtc agcttccaga caccgcatga cgccgcgcac cagtaccgga acgccaccct
     3961 gccagacaac gcggtctgga catcgtcggt ctgcgctgat tgcaaagccg cgcccctggt
     4021 gatcagcaag gcaccggtca agggcaagcc gcgacgggag atcgtcgtct cctgcccgtc
     4081 ctgcgactcc agcgcatcag ccaggcccgg aggcaggaca tggacagacg agcacatcgc
     4141 ggtgcccgag ttcgcgcaga acgagacgcg cagcctggcg aagatccccg ttattttgcc
     4201 gcagggggag cagccgcagc tgctcaaaag accagggaca gggcgcggtc caggacgccc
     4261 ccgcaagcgc atccccctga ccctcgaccg ggtaccagag agccggttca accgcctcgc
     4321 cctttccgtc cttgccgccg tcgggcgctg ggactacatc aacgacatca tccggcgcat
     4381 cgatcggaac cgcggcgacg ccgccaccct gatcgccctt gccgaccaca tccggaccaa
     4441 ccgaccagag agagatttat gaccgaaacc acccgtccga tcctgaccat caagcgacgc
     4501 aaccgcacgg tctacgtccc gccggaaaag gccgcgaaat ctgagccgca gccagccgaa
     4561 cggactacca gcaaggcgaa ggtacagcca aaagcgaaag ccccgcagaa gcccgcacag
     4621 aagcccacag cggcaaagcc gaaaaagacg cctgaacagg tcgccgaggg gatccgcaga
     4681 caggaggaga acgaccgccg ccagcaggac atcgcgatcg ccaacaagtc caaaaaggcg
     4741 ctggccaacc gggtgaccaa agcattcctg aaagagcact ggcctgactt gctgacccgg
     4801 ggagttgacc gtcgccccat ggcaaagggc atcatcgagc agatcctgac ccgaggcgca
     4861 gagctgaaca tgccgccaga ggcagaccgc aaagccgtcc gtcgcatcct gggcattgtc
     4921 acgggtacga gctcgtacca gcagagcctc atcgaccagt gcatgcgcta cgacctggac
     4981 ggcaacccca ccgaaccagc aaccccggag gaggccgaaa acgccaaagc ccgcctgaca
     5041 gagatcaagg cattccggcg caaggcacgc caggcgaagg gcgaaaaaaa gggcgcataa
     5101 cgcgccccgt ctcacccagc accatgtagg cgcagcacga cgttcagcag cacggcgagc
     5161 agctgcgaca cgttgcgcga tccccctggc ctttcgcgca gcaacaaccc cgtcagggat
     5221 caccacctca gggatgacca gacgcgagac ggcaaactca ctcatcatca gcccccagga
     5281 ccgcaccaat agcacgcccg aaagcatgcc gatcaagcgc gtctgaggcc gcagcaacac
     5341 ctgcgtctgt ctcctgtaaa taccgggcgc ggcggctctt cagatacgcc ccccagcgat
     5401 acagcgacgg ctcatgtaac gcgaacgcgt gcggttgccc ggccttgatc agcgattcct
     5461 gatacagcgc cagcttcgat cgttcaccat catccctgtc accaggacga gcctccagaa
     5521 caggcagcaa cgagatcagc gcaagacggt ggccagcaga gtaacaacca cctgccagcg
     5581 gcatccgcgt cttaccctgc aggtggcgcg gcatcggctt tactcgtccc aattgatacc
     5641 ccgggcagca ggggagagag acgcctgttg ctgcacctga gcacgcaccg cagcgaacgc
     5701 cggagcacgc gacgcatcag ggagtccgcg agaggaggcc agcacgccgt caagcatcac
     5761 cagatcggca ggagccacgc ccgcctgttt catctcagtc aggaacgccg catcctggat
     5821 ggactgagca cgcgcccggt gaaccgactc cgtaggagag gcaaccggag ccccgatcgg
     5881 cgcgaacgtc tgcgcagcac cgcccgtctc cagcacctgc gccagcgcgt cgccggcccc
     5941 ggtagatttt gccagcgtcg cctcatggcg aagccggtca cacagcatcg aaaatgcaga
     6001 catttacgcc tccatgccgc gattgcggcg cagaatttcg atcgcaccct gcgtggagaa
     6061 cagctggttc aggcggcgat caggattgat aacagtacgg tacaaactgc gccccgcgtc
     6121 ggactcggca agcagcgtcg cctcatccat cgccagcttc agcagctgct caatacgctg
     6181 tacgcgcacg gcgtgcgcca ggttgaacag gccgatcgca gccgccgcat cacggcggtc
     6241 agcatcctgc agcagcgcgt cacgctcccg ggccacatca gcctcagcag cctcggcggc
     6301 aaccagctcg gcctcgatat ccggcagcag gaacgcagcg acgcccgcat cggggctcac
     6361 agtctcgcca gcacgcagcg ccgtcttgcc gtcggcaatc agcccgcgca ggcgttcagc
     6421 ttccgtcctg agcccttcca ccacgcgagc ggcgtcaatg cgtcgcgttt ccacgtcggc
     6481 tgctgcctga cgcatttcac gcgccacccg gcgatcgtca agcgcggtaa gttgagcgga
     6541 ggagttagga tccaccgaca ggcaaggaag gtcataaacg ggaaaatggt cactcgccat
     6601 ttcagcaagc gtggtacgca gccaggtcgc gtccgggcat tcggttttga tttcggtcat
     6661 ggtttggttc tcagcaagtt atcagcaaag cggcagcaaa cgccgctcag acagcatgcc
     6721 gtcacgacac cgcgcagaca tcaacgcaga gggcgcaggg ctctcgccca gcgcggagag
     6781 ccgcctggag gccgtcgcta atcccctaca ttccgggaca cccgacaaag cgacgaaata
     6841 gacactttag cgatagactt tcggtacagt tgtggcatgt tgtgtcgaaa gagacgtatc
     6901 ggtcagagga gagaaaaaag tgagcaaaac atacggttac gttcgcgtat caaccgacga
     6961 acaaaccagc aacagccagc gcgacgccat catcgcccgg ttcccaacag cctccatcct
     7021 cgccgacgac gggatcagcg ggacggtacc accacgcgag cgtccagccc tcggcatcct
     7081 cctgaaccag atcctgcagg agggcgacac gctggtcatc tacgcgatcg accgcatcgg
     7141 caggaacgcc cgggacgccc tcgaactggt cgaggagctg gagaaacgaa aagtaaccct
     7201 ggtcagcatc cgcgagggtg tcgatttgag cagcgccgtc ggacaggccg tcctcggcat
     7261 catggcgagt ttcgcgcaga tggagcgcaa gctcatactg gaacgccagg cggcagggat
     7321 cgaggccgca aagagccgtg gccagaagtt cggacgcccg accaagatcg gcgcgaaaga
     7381 agcgggggag atagtcatgc gccgcgccat gggggtacca atagccacca tagccaagca
     7441 gttaaatctg agcaggagcg cgatttaccg ccaccttgag aaggtcgacg cggtagcgcc
     7501 ggagtaagag agagcgcagc ggtgtaggtg gctccaacca ttccacaccg ccgcaaccca
     7561 gcccggggaa cgcatgagga gcagacccgg gcaccattcc caacaaggaa cagaaccatg
     7621 ataaaccaag cagcaacacc gcgctatccc ggcctgagca ttgaacacgg cgagatccgc
     7681 cagttcggca agccccgcca ttgcgccgcc aaccgcaaag gccagtacca gaccaccctc
     7741 cgcgacagcg ccggtcggta ccagacagtc agcatcatca gcctttacgc ctgggaagca
     7801 tgaccataac gaaagcctta caggtgtaag cagcaagcaa taacaaaagc cttacgccat
     7861 tacgaaccac ttacaggggc gcgggagcgc ccccagaaac cagaatcata aggagagaac
     7921 gatggcattt gaaaacattc cccgtaggtt ccagagatgg aaggtaatca tggttcccgg
     7981 agcaaagtca tatgagtcat gggacgagat ggttaccggc gagaacttct ccgaagaaac
     8041 caggatgaaa acgtacaaaa agcaccgcac caaggccatc gaccgcaccg tggcaaacca
     8101 ggagtacatc cataccatga cggaggacgg gatcaccgtc aggtaccgcc tcgaacgctg
     8161 gcgctaaccc cagctaggag cgcagcgact gctgcgcctc ctcccttacc cagaacgcac
     8221 cagagccatt ctaagcgcct cagccacaaa aatacccaac cgcacgacca cgcacgcaca
     8281 aacgccgtgt accccccaaa aacccccgcc attttcagcg cgtgacgttt caatacagga
     8341 gagaaaccaa ccggagcagg acaccatgaa gaaatacacc accgagcaga aagcccaggc
     8401 gctcagacta ctggagcagg acggcgcaac cgcagccacc gtcgcacgca ccatggggat
     8461 cccaccccgc accgtcagag attgggcgaa agcccgcacc gacgcaccca gcaacgtcct
     8521 cagcatcgag gagatgcgcg aacgcgcaca gcgagccgtc gaggccaccc cacaggcagc
     8581 gatccgacgc ctaaaaaacc actttgtaca gcgccagttt gagctgctac agcgccacgc
     8641 caccgacctg caagccctcc gcacagcttc gctacaggcc atgctggaga aggacgccac
     8701 catggtcaaa gcgatcagcg gccttatgac atccctactc aagacgcagg aacgcgagcg
     8761 cgtcatctac gagatcaagc caggcaccga ggccgacatc atgcgcgagg gcatgaatcg
     8821 ccaacagagc tgaaaacggc gggaaaaccg ccttttaccg ccgctttacc gccgcagaaa
     8881 acccgggagc ccgcgaaata cgcggatccg cacgttaacg agttgttacc gctcgcgtgc
     8941 gcgagggcaa gccctcacgc agaaccaaca gacgaccacc cagcgcgagg cgaaagcctc
     9001 gtgccctcag cgccacccaa acctccgatc cccctcagaa ccactaccac cgaccatccc
     9061 ggcacgacga acccatccac cgatctaata agacaagcaa agtgcatccg gcagggtgca
     9121 gctgcggcgc gtcgattcca agatgatcca cccgttccac ccgatccggc atagattcat
     9181 aaatagtaaa aaaaacaact aattgttcaa ttagtgagca tttaccccct atttacccaa
     9241 tttattcatt ttttgttttt aattagaaaa tagggtggaa cgggtggaac acaacaatga
     9301 aagcctttaa acacgaggcc ttagctgttc catccacggg cggaacacgg gtggaacggg
     9361 tggaacaccc gggtggaaca ggtggtacaa aaacggcgtg atgatccacc cgtgatccac
     9421 ccgggtggaa caataaaaaa ggggagcata tagctcccct cttacggaaa agtaaccctt
     9481 acacgacagc ttgcgttcga cggaagtagc cacgaactaa cgcaccgttt atgcgcttcc
     9541 tgccaacctc atagcccaat cgggacatga tagccgccat gcgctgatgc tgcttgttgc
     9601 tggtatggcc aatcatgagc cctacctgag agaacaaggc cttcgtcggc acgaaagcag
     9661 tcgggtcgca gtcgtcaacg tccagcttga gcagctcggc gatctcgtct tcccacggat
     9721 cctcgacgcg gaactcgtca tgcaccttac gcgccagcgt ttcagcggcc tcccacatca
     9781 ccccattctc attgaacagg tggagcgctt cagcccacag ctgcgagcgg tttttctcga
     9841 tggccgcgca gtccacgtcc ccggaattca tgggcaaata gcggcggttc cccgtcgagt
     9901 cattgaggat ctgcttgttg ttcgtggtac cgatgaacac cacgcggcgc gggtttcgca
     9961 ccttgaactc tttaaatttc ggcacccact cgtcgtgggc agcggagaag aacgctttca
    10021 ccgattcgga gtcgcgcccg tggaggccgc gcagctcctc cagctcgcca atcaggcacc
    10081 cacgcatacg gcgcactagg gagtcgtcct tgtcgttcag gttgaagctg gagtaggagt
    10141 cagggaatgg cgacataagg cgaacgcccg tcgatttgcg ggcaccctgt cgcccgtaca
    10201 gcagcggaac catgtcggcc ttgattccgg gattcattac gcgcccggca agcgccgtcc
    10261 aggtgtagag gctgaccgcc gccaggtact cgggatcccc ttggcatttg aagcaccgct
    10321 cgaagaagta actgacgcga ggtttgccgt cccattcctg agccgtcagc caacgctggg
    10381 cgctgtcgaa tttgttctca tcgcagacgt agaacatggc gtcgcgggca ttgtccttgg
    10441 tcaccttctc gaagtcggca gcagcgaggc gcagacgcag ctcggtaact aggttgtcgg
    10501 acagcgggta ccattgcccg ttcacgaacg ccccctcctc cgcaaagaac tcatcgaaag
    10561 cgagctggat gccggtgacg gaatggcagc gcatagcggc gacaacgttc tcaagagagg
    10621 acgagtagcg ccccttccgg tagttgatct ccagctccac gggaaacgcg gtagtcgggt
    10681 attttttttg tagagacggg cgtcccacct tggcagcggc gatcggcgtt acgttgtcgg
    10741 cagcagtgcc ggaattttca tcaatttgtg acacgcaatt actcctttaa aaacgtagga
    10801 gtcggcgtca ggcgggggct tacgagggaa attcacactt gcgcgatctt atcgacgtgg
    10861 tacacttctc tttgagtttg aggagtacgc tgcgcaagtg ctattcttcc ttcgagaacg
    10921 tcccgctaag acgtgactcg aattaatcgg aggagttacc agctcccccg gcagaaaaag
    10981 gtgaccaccc aacaggtcgc ctttttttgt gcccgaagat ccccaaccgg atccgcagac
    11041 gccagatcag cttacccgat cccaaccctc cacgccactt agcgccgcgc acttatcgcg
    11101 ctgtcgtgat cacgcccagc tcaatctctg cgccgcctct gctatttatc catgtacaca
    11161 aacggcaccc cggcgacgca aagccgtcgc ccctctgtgc acgacaactg cacgacgtcg
    11221 aattcgacgc gcacgacaaa acgcattaca ctacgcaaga caaacagcac gacaaaacgc
    11281 acgacgtgga gagcagcagc catgagagag aaaaccaaaa ccctgaccgt tcgcctaccc
    11341 gttagcctga tcgaacgcat cgagcagaac gcagcacgaa ccggagcaac ccgcacggat
    11401 gctgtcatag aggcgctgaa cgccggactt ggcacgaagc cggaagggat ggacaccaaa
    11461 cttttagctg ccatcgaagc gctgagggct gaagtcaggg cgctgtccgt cggcggtaaa
    11521 cctgcacgac aaaccgcaca acaaacagca ccacaaagcg cacaacaacc tgcaagacag
    11581 atagtccccc ggggagccga tccgcgcttc caggccgtca gtgatgaggc tgaccagctc
    11641 attgtcagca tgaccgcaga gaacgcgact ctgcccgcca tcgcgtcggc actgaacgca
    11701 gcagggcacc ggacagcgac gggacaggag tggaacacca gccgtgtccg ggactatcgc
    11761 cataagttgc gggaaagtgg gctactgaat tagcagtcct aagtgacata gctaaatatg
    11821 ataaaattcg aacataaaaa ttttacaagg aaaagaagtg gctatcggtg atgatgctat
    11881 ccgtgacgct ttctatgctt ttacacagca agctgaagaa atggatgata aaggtctccc
    11941 tcaggaagtt tgggagacgc cttgttttac ctatctgatg actaagaagc agttcaatca
    12001 aatgaaagtt gtttgtcaga gaaacggctg ggacatacct gcatcaccag caataccgat
    12061 cacttgggca atgtttaggc atgtgctatc tgctcgtaac tcgaaggata agctaagttg
    12121 gcaagagtgt gcggagattt tggcaactgc attctcagtg cagagcaatg tgtacgttaa
    12181 cagagattac tcggagcaga ctattgtgct caatgctgtt cgtcgcatta acgttgctgg
    12241 ggctggcttt ttcgcaatgg cgatagttga tgtctctgaa aataatttgg cccccgtgac
    12301 tgcataccat gcaacggagg ccaaatgtaa agctatacag cgagggtgat aggaaggacg
    12361 cccatttaag caattctcct caatttaatg agaatgaacc ataacgccta aatatttccc
    12421 ttcattggtt cctttcggat ctagcagcat cggcggtttt ctgctgtcta tcaaattcac
    12481 tgttattact aggaagccgc aacataactt cccaacagga gaccagcctc ctgactgccc
    12541 agcgcaaagg gttaattact ggttaatacc ccctgcgaat gctcttgcag tacaacaata
    12601 gtaggctatt gttgacgtag attcaactgg tttgtttata accaagcggc acgaggagcg
    12661 ggtttacagg tttttatagc tctttagagg cggttaacca tattgcgata tcaggtaatt
    12721 atagatcttg aaatcattaa atttatcaat tatgattaat tttatgatta atttttgatc
    12781 tatagaggta ctttgatgga tattacgata tctgacgcta catatagccg tcttgctgca
    12841 caagcaaagg gatttgaaac tcccgaacag gtaataaac
//
