LOCUS       NZ_OX291706            12879 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1052 plasmid 2, complete sequence.
ACCESSION   NZ_OX291706
VERSION     NZ_OX291706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201021
            Assembly: GCF_946411625.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 08:21:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,245
            CDSs (total)                      :: 5,114
            Genes (coding)                    :: 5,041
            CDSs (with protein)               :: 5,041
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 29 of 74
            Pseudo Genes (incomplete)         :: 44 of 74
            Pseudo Genes (internal stop)      :: 15 of 74
            Pseudo Genes (multiple problems)  :: 11 of 74
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1052"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="2"
     gene            complement(63..647)
                     /locus_tag="N4J68_RS26105"
                     /old_locus_tag="SAMEA13201021_05183"
     CDS             complement(63..647)
                     /locus_tag="N4J68_RS26105"
                     /old_locus_tag="SAMEA13201021_05183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351424.1"
                     /translation="MDITISDATYSRLAAQAKGFETPEQVINRLIDVVEIDHDTRPELF
                     FNPEGEADFKKALLKSKQAKVEMFKRDGSCETGVWNARAISEESSVRANIWSGYLRGWK
                     EKGIVRAVFTVQEVPDLPGAAESVQWNDYTISRTFSGGISVQRNGMPVTIVKPFLRDVA
                     HDLKISLLNSNGNEMNTRQLGAAIIKKLNNS"
                     /name="hypothetical protein"
     gene            complement(1094..1585)
                     /locus_tag="N4J68_RS26110"
     CDS             complement(1094..1585)
                     /locus_tag="N4J68_RS26110"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(1650..2141)
                     /locus_tag="N4J68_RS26115"
     CDS             complement(1650..2141)
                     /locus_tag="N4J68_RS26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558888.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGT
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQTAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            2669..3964
                     /locus_tag="N4J68_RS26120"
                     /old_locus_tag="SAMEA13201021_05185"
     CDS             2669..3964
                     /locus_tag="N4J68_RS26120"
                     /old_locus_tag="SAMEA13201021_05185"
                     /inference="COORDINATES: protein motif:HMM:NF017116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_119879886.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKLDVDDCDPTAFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            complement(4610..5077)
                     /locus_tag="N4J68_RS26125"
                     /old_locus_tag="SAMEA13201021_05186"
     CDS             complement(4610..5077)
                     /locus_tag="N4J68_RS26125"
                     /old_locus_tag="SAMEA13201021_05186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_070559148.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATAATVARTMGIPPRTVRDWAKART
                     DAPSNVLSIEEMRERAQRAVEATPQAAIRRLKNHFVQRQFELLQRHATDLQALRTASLQ
                     AMLEKDATMVKAISGLMTSLLKTQERERVIYEIKPGTEADIMREGMNRQQS"
                     /name="transposase"
     gene            complement(5276..5521)
                     /locus_tag="N4J68_RS26130"
                     /old_locus_tag="SAMEA13201021_05187"
     CDS             complement(5276..5521)
                     /locus_tag="N4J68_RS26130"
                     /old_locus_tag="SAMEA13201021_05187"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558916.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTVANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(5640..5825)
                     /locus_tag="N4J68_RS26135"
                     /old_locus_tag="SAMEA13201021_05188"
     CDS             complement(5640..5825)
                     /locus_tag="N4J68_RS26135"
                     /old_locus_tag="SAMEA13201021_05188"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879885.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDSA
                     GRYQTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            complement(5936..6523)
                     /locus_tag="N4J68_RS26140"
                     /old_locus_tag="SAMEA13201021_05189"
     CDS             complement(5936..6523)
                     /locus_tag="N4J68_RS26140"
                     /old_locus_tag="SAMEA13201021_05189"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181654175.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQILQEGDTLVIYAIDRIGRNARDALELVEELEKRKVTLVSIREGVDLS
                     SAVGQAVLGIMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            6783..7439
                     /locus_tag="N4J68_RS26145"
                     /old_locus_tag="SAMEA13201021_05190"
     CDS             6783..7439
                     /locus_tag="N4J68_RS26145"
                     /old_locus_tag="SAMEA13201021_05190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879883.1"
                     /translation="MTEIKTECPDATWLRTTLAEMASDHFPVYDLPCLSVDPNSSAQLT
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLRTEAERLRGLIADGKTALRAGETVSPD
                     AGVAAFLLPDIEAELVAAEAAEADVARERDALLQDADRRDAAAAIGLFNLAHAVRVQRI
                     EQLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEA"
                     /name="hypothetical protein"
     gene            7440..7823
                     /locus_tag="N4J68_RS26150"
                     /old_locus_tag="SAMEA13201021_05191"
     CDS             7440..7823
                     /locus_tag="N4J68_RS26150"
                     /old_locus_tag="SAMEA13201021_05191"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559064.1"
                     /translation="MSAFSMLCDRLRHEATLAKSTGAGDALAQVLETGGAAQTFAPIGA
                     PVASPTESVHRARAQSIQDAAFLTEMKQAGVAPADLVMLDGVLASSRGLPDASRAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            7829..8185
                     /locus_tag="N4J68_RS26155"
     CDS             7829..8185
                     /locus_tag="N4J68_RS26155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559062.1"
                     /translation="MPRHLQGKTRMPLAGGCYSAGHRLALISLLPVLEARPGDRDDGER
                     SKLALYQESLIKAGQPHAFALHEPSLYRWGAYLKSRRARYLQETDAGVAAASDALDRHA
                     FGRAIGAVLGADDE"
                     /name="hypothetical protein"
     gene            complement(8343..8984)
                     /locus_tag="N4J68_RS26160"
                     /old_locus_tag="SAMEA13201021_05192"
     CDS             complement(8343..8984)
                     /locus_tag="N4J68_RS26160"
                     /old_locus_tag="SAMEA13201021_05192"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_070559060.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELNMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(8981..10270)
                     /locus_tag="N4J68_RS26165"
                     /old_locus_tag="SAMEA13201021_05193"
     CDS             complement(8981..10270)
                     /locus_tag="N4J68_RS26165"
                     /old_locus_tag="SAMEA13201021_05193"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157698538.1"
                     /translation="MNDDNDTTEATQPSTEALKCVGAAERFPDIMPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQGEQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRNRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            10626..10835
                     /locus_tag="N4J68_RS26170"
     CDS             10626..10835
                     /locus_tag="N4J68_RS26170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765954.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPVLKRFIADQRRPGRAPYGVR"
                     /name="hypothetical protein"
     gene            10822..12003
                     /locus_tag="N4J68_RS26175"
                     /old_locus_tag="SAMEA13201021_05194"
     CDS             10822..12003
                     /locus_tag="N4J68_RS26175"
                     /old_locus_tag="SAMEA13201021_05194"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765953.1"
                     /translation="MAFDNSHLTDAGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSTRHNRGSAHHRSQYRHFLKLKGVTNPEAMAQVFLRFSGEVYWRLDSNR
                     TPPSTEQVAALAERKRRSIEAVARREDADRRKADKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDTSPHAPYSPLFVCEGFATAVALH
                     HLTKGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPV
                     PTIPGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            12229..12687
                     /locus_tag="N4J68_RS26180"
                     /old_locus_tag="SAMEA13201021_05195"
     CDS             12229..12687
                     /locus_tag="N4J68_RS26180"
                     /old_locus_tag="SAMEA13201021_05195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765952.1"
                     /translation="MKSKTELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNTLATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDGAAA"
                     /name="hypothetical protein"
ORIGIN
        1 cgcagtagaa ctctgaccgg cggcggcgtt gagccgtcgc tcctctgcgt gaagcatcga
       61 tgttaagagt tatttaactt ctttatgatc gctgcgccaa gctgccgagt attcatttca
      121 tttccattgc tatttagcaa tgatattttt aaatcatgcg caacatcacg tagaaatggc
      181 ttcacaatag ttacaggcat gccatttctt tgaactgaaa taccgccact aaaagtgcga
      241 ctgattgtat aatcattcca ctgcacactt tcagccgctc ctggcaagtc aggaacctcc
      301 tggacagtga aaaccgcacg aacaatccct ttttccttcc atccacgcaa atacccactc
      361 caaatatttg cccgaactga tgattcttct gaaatcgcac gagcattcca tactccagtt
      421 tcacaggaac catccctttt aaacatttcg actttggcct gttttgattt tagtaacgct
      481 tttttaaaat ccgcttcacc ttcaggatta aaaaaaagtt cagggcgtgt gtcatgatcg
      541 atttcaacaa catcgatcag acggtttatt acctgttcgg gagtttcaaa tccctttgct
      601 tgtgcagcaa gacggctata tgtagcgtca gatatcgtaa tatccatcaa agtacctcta
      661 tagatcaaaa attaatcata aaattaatca taattgataa atttaatgat ttcaagatct
      721 ataattacct gatatcgcaa tatggttaac cgcctctaaa gagctataaa aacctgtaaa
      781 cccgctcctc gtgccgcttg gttataaaca aaccagttga atctacgtca acaatagcct
      841 actattgttg tactgcaaga gcattcgcag ggggtattaa ccagtaatta accctttgcg
      901 ctgggcagtc aggaggctgg tctcctgttg ggaagttatg ttgcggcttc ctagtaataa
      961 cagtgaattt gatagacagc agaaaaccgc cgatgctgct agatccgaaa ggaaccaatg
     1021 aagggaaata tttaggcgtt atggttcatt ctcattaaat tgaggagaat tgcttaaatg
     1081 ggcgtccttc ctatcaccct cgctgtatag ctttacattt ggcctccgtt gcatggtatg
     1141 cagtcacggg ggccaaatta ttttcagaga catcaactat cgccattgcg aaaaagccag
     1201 ccccagcaac gttaatgcga cgaacagcat tgagcacaat agtctgctcc gagtaatctc
     1261 tgttaacgta cacattgctc tgcactgaga atgcagttgc caaaatctcc gcacactctt
     1321 gccaacttag cttatccttc gagttacgag cagatagcac atgcctaaac attgcccaag
     1381 tgatcggtat tgctggtgat gcaggtatgt cccagccgtt tctctgacaa acaactttca
     1441 tttgattgaa ctgcttctta gtcatcagat aggtaaaaca aggcgtctcc caaacttcct
     1501 gagggagacc tttatcatcc atttcttcag cttgctgtgt aaaagcatag aaagcgtcac
     1561 ggatagcatc atcaccgata gccacttctt ttccttgtaa aatttttatg ttcgaatttt
     1621 atcatattta gctatgtcac ttaggactgc taattcagta gcccactttc ccgcaactta
     1681 tggcgatagt cccggacacg gctggtgttc cactcctgtc ccgtcgctgt ccggtgccct
     1741 gctgcgttca gtgccgacgc gatggcgggc agagtcgcgt tctctgcggt catgctgaca
     1801 atgagctggt cagcctcatc actgacggcc tggaagcgcg gatcggctcc ccgggggact
     1861 atctgtcttg caggttgttg tgcgctttgt ggtgctgttt gttgtgcggt ttgtcgtgca
     1921 ggtttaccgc cgacggacag cgccctgact tcagccctca gcgcttcgat ggcagctaaa
     1981 agtttggtgt ccatcccttc cggcttcgtg ccaagtccgg cgttcagcgc ctctatgaca
     2041 gcatccgtgc gggttgctcc ggttcgtgct gcgttctgct cgatgcgttc gatcaggcta
     2101 acgggtaggc gaacggtcag ggttttggtt ttctctctca tggctgctgc tctccacgtc
     2161 gtgcgttttg tcgtgctgtt tgtcttgcgt agtgtaatgc gttttgtcgt gcgcgtcgaa
     2221 ttcgacgtcg tgcagttgtc gtgcacagag gggcgacggc tttgcgtcgc cggggtgccg
     2281 tttgtgtaca tggataaata gcagaggcgg cgcagagatt gagctgggcg tgatcacgac
     2341 agcgcgataa gtgcgcggcg ctaagtggcg tggagggttg ggatcgggta agctgatctg
     2401 gcgtctgcgg atccggttgg ggatcttcgg gcacaaaaaa aggcgacctg ttgggtggtc
     2461 acctttttct gccgggggag ctggtaactc ctccgattaa ttcgagtcac gtcttagcgg
     2521 gacgttctcg aaggaagaat agcacttgcg cagcgtactc ctcaaactca aagagaagtg
     2581 taccacgtcg ataagatcgc gcaagtgtga atttccctcg taagcccccg cctgacgccg
     2641 actcctacgt ttttaaagga gtaattgcgt gtcacaaatt gatgaaaatt ccggcactgc
     2701 tgccgacaac gtaacgccga tcgccgctgc caaggtggga cgcccgtctc tacaaaaaaa
     2761 atacccgact accgcgtttc ccgtggagct ggagatcaac taccggaagg ggcgctactc
     2821 gtcctctctt gagaacgttg tcgccgctat gcgctgccat tccgtcaccg gcatccagct
     2881 cgctttcgat gagttctttg cggaggaggg ggcgttcgtg aacgggcaat ggtacccgct
     2941 gtccgacaac ctagttaccg agctgcgtct gcgcctcgct gctgccgact tcgagaaggt
     3001 gaccaaggac aatgcccgcg acgccatgtt ctacgtctgc gatgagaaca aattcgacag
     3061 cgcccagcgt tggctgacgg ctcaggaatg ggacggcaaa cctcgcgtca gttacttctt
     3121 cgagcggtgc ttcaaatgcc aaggggatcc cgagtacctg gcggcggtca gcctctacac
     3181 ctggacggcg cttgccgggc gcgtaatgaa tcccggaatc aaggccgaca tggttccgct
     3241 gctgtacggg cgacagggtg cccgcaaatc gacgggcgtt cgccttatgt cgccattccc
     3301 tgactcctac tccagcttca acctgaacga caaggacgac tccctagtgc gccgtatgcg
     3361 tgggtgcctg attggcgagc tggaggagct gcgcggcctc cacgggcgcg actccgaatc
     3421 ggtgaaagcg ttcttctccg ctgcccacga cgagtgggtg ccgaaattta aagagttcaa
     3481 ggtgcgaaac ccgcgccgcg tggtgttcat cggtaccacg aacaacaagc agatcctcaa
     3541 tgactcgacg gggaaccgcc gctatttgcc catgaattcc ggggacgtgg actgcgcggc
     3601 catcgagaaa aaccgctcgc agctgtgggc tgaagcgctc cacctgttca atgagaatgg
     3661 ggtgatgtgg gaggccgctg aaacgctggc gcgtaaggtg catgacgagt tccgcgtcga
     3721 ggatccgtgg gaagacgaga tcgccgagct gctcaagctg gacgttgacg actgcgaccc
     3781 gactgctttc gtgccgacga aggccttgtt ctctcaggta gggctcatga ttggccatac
     3841 cagcaacaag cagcatcagc gcatggcggc tatcatgtcc cgattgggct atgaggttgg
     3901 caggaagcgc ataaacggtg cgttagttcg tggctacttc cgtcgaacgc aagctgtcgt
     3961 gtaagggtta cttttccgta agaggggagc tatatgctcc ccttttttat tgttccaccc
     4021 gggtggatca cgggtggatc atcacgccgt ttttgtacca cctgttccac ccgggtgttc
     4081 cacccgttcc acccgtgttc cgcccgtgga tggaacagct aaggcctcgt gtttaaaggc
     4141 tttcattgtt gtgttccacc cgttccaccc tattttctaa ttaaaaacaa aaaatgaata
     4201 aattgggtaa atagggggta aatgctcact aattgaacaa ttagttgttt tttttactat
     4261 ttatgaatct atgccggatc gggtggaacg ggtggatcat cttggaatcg acgcgccgca
     4321 gctgcaccct gccggatgca ctttgcttgt cttattagat cggtggatgg gttcgtcgtg
     4381 ccgggatggt cggtggtagt ggttctgagg gggatcggag gtttgggtgg cgctgagggc
     4441 acgaggcttt cgcctcgcgc tgggtggtcg tctgttggtt ctgcgtgagg gcttgccctc
     4501 gcgcacgcga gcggtaacaa ctcgttaacg tgcggatccg cgtatttcgc gggctcccgg
     4561 gttttctgcg gcggtaaagc ggcggtaaaa ggcggttttc ccgccgtttt cagctctgtt
     4621 ggcgattcat gccctcgcgc atgatgtcgg cctcggtgcc tggcttgatc tcgtagatga
     4681 cgcgctcgcg ttcctgcgtc ttgagtaggg atgtcataag gccgctgatc gctttgacca
     4741 tggtggcgtc cttctccagc atggcctgta gcgaagctgt gcggagggct tgcaggtcgg
     4801 tggcgtggcg ctgtagcagc tcaaactggc gctgtacaaa gtggtttttt aggcgtcgga
     4861 tcgctgcctg tggggtggcc tcgacggctc gctgtgcgcg ttcgcgcatc tcctcgatgc
     4921 tgaggacgtt gctgggtgcg tcggtgcggg ctttcgccca atctctgacg gtgcggggtg
     4981 ggatccccat ggtgcgtgcg acggtggctg cggttgcgcc gtcctgctcc agtagtctga
     5041 gcgcctgggc tttctgctcg gtggtgtatt tcttcatggt gtcctgctcc ggttggtttc
     5101 tctcctgtat tgaaacgtca cgcgctgaaa atggcggggg tttttggggg gtacacggcg
     5161 tttgtgcgtg cgtggtcgtg cggttgggta tttttgtggc tgaggcgctt agaatggctc
     5221 tggtgcgttc tgggtaaggg aggaggcgca gcagtcgctg cgctcctagc tggggttagc
     5281 gccagcgttc gaggcggtac ctgacggtga tcccgtcctc cgtcatggta tggatgtact
     5341 cctggtttgc cacggtgcgg tcgatggcct tggtgcggtg ctttttgtac gttttcatcc
     5401 tggtttcttc ggagaagttc tcgccggtaa ccatctcgtc ccatgactca tatgactttg
     5461 ctccgggaac catgattacc ttccatctct ggaacctacg gggaatgttt tcaaatgcca
     5521 tcgttctctc cttatgattc tggtttctgg gggcgctccc gcgcccctgt aagtggttcg
     5581 taatggcgta aggcttttgt tattgcttgc tgcttacacc tgtaaggctt tcgttatggt
     5641 catgcttccc aggcgtaaag gctgatgatg ctgactgtct ggtaccgacc ggcgctgtcg
     5701 cggagggtgg tctggtactg gcctttgcgg ttggcggcgc aatggcgggg cttgccgaac
     5761 tggcggatct cgccgtgttc aatgctcagg ccgggatagc gcggtgttgc tgcttggttt
     5821 atcatggttc tgttccttgt tgggaatggt gcccgggtct gctcctcatg cgttccccgg
     5881 gctgggttgc ggcggtgtgg aatggttgga gccacctaca ccgctgcgct ctctcttact
     5941 ccggcgctac cgcgtcgacc ttctcaaggt ggcggtaaat cgcgctcctg ctcagattta
     6001 actgcttggc tatggtggct attggtaccc ccatggcgcg gcgcatgact atctcccccg
     6061 cttctttcgc gccgatcttg gtcgggcgtc cgaacttctg gccacggctc tttgcggcct
     6121 cgatccctgc cgcctggcgt tccagtatga gcttgcgctc catctgcgcg aaactcgcca
     6181 tgatgccgag gacggcctgt ccgacggcgc tgctcaaatc gacaccctcg cggatgctga
     6241 ccagggttac ttttcgtttc tccagctcct cgaccagttc gagggcgtcc cgggcgttcc
     6301 tgccgatgcg gtcgatcgcg tagatgacca gcgtgtcgcc ctcctgcagg atctggttca
     6361 ggaggatgcc gagggctgga cgctcgcgtg gtggtaccgt cccgctgatc ccgtcgtcgg
     6421 cgaggatgga ggctgttggg aaccgggcga tgatggcgtc gcgctggctg ttgctggttt
     6481 gttcgtcggt tgatacgcga acgtaaccgt atgttttgct cacttttttc tctcctctga
     6541 ccgatacgtc tctttcgaca caacatgcca caactgtacc gaaagtctat cgctaaagtg
     6601 tctatttcgt cgctttgtcg ggtgtcccgg aatgtagggg attagcgacg gcctccaggc
     6661 ggctctccgc gctgggcgag agccctgcgc cctctgcgtt gatgtctgcg cggtgtcgtg
     6721 acggcatgct gtctgagcgg cgtttgctgc cgctttgctg ataacttgct gagaaccaaa
     6781 ccatgaccga aatcaaaacc gaatgcccgg acgcgacctg gctgcgtacc acgcttgctg
     6841 aaatggcgag tgaccatttt cccgtttatg accttccttg cctgtcggtg gatcctaact
     6901 cctccgctca acttaccgcg cttgacgatc gccgggtggc gcgtgaaatg cgtcaggcag
     6961 cagccgacgt ggaaacgcga cgcattgacg ccgctcgcgt ggtggaaggg ctcaggacgg
     7021 aagctgaacg cctgcgcggg ctgattgccg acggcaagac ggcgctgcgt gctggcgaga
     7081 ctgtgagccc cgatgcgggc gtcgctgcgt tcctgctgcc ggatatcgag gccgagctgg
     7141 ttgccgccga ggctgctgag gctgatgtgg cccgggagcg tgacgcgctg ctgcaggatg
     7201 ctgaccgccg tgatgcggcg gctgcgatcg gcctgttcaa cctggcgcac gccgtgcgcg
     7261 tacagcgtat tgagcagctg ctgaagctgg cgatggatga ggcgacgctg cttgccgagt
     7321 ccgacgcggg gcgcagtttg taccgtactg ttatcaatcc tgatcgccgc ctgaaccagc
     7381 tgttctccac gcagggtgcg atcgaaattc tgcgccgcaa tcgcggcatg gaggcgtaaa
     7441 tgtctgcatt ttcgatgctg tgtgaccggc ttcgccatga ggcgacgctg gcaaaatcta
     7501 ccggggccgg cgacgcgctg gcgcaggtgc tggagacggg cggtgctgcg cagacgttcg
     7561 cgccgatcgg ggctccggtt gcctctccta cggagtcggt tcaccgggcg cgtgctcagt
     7621 ccatccagga tgcggcgttc ctgactgaga tgaaacaggc gggcgtggct cctgccgatc
     7681 tggtgatgct tgacggcgtg ctggcctcct ctcgcggact ccctgatgcg tcgcgtgctc
     7741 cggcgttcgc tgcggtgcgt gctcaggtgc agcaacaggc gtctctctcc cctgctgccc
     7801 ggggtatcaa ttgggacgag taaagccgat gccgcgccac ctgcagggta agacgcggat
     7861 gccgctggca ggtggttgtt actctgctgg ccaccgtctt gcgctgatct cgttgctgcc
     7921 tgttctggag gctcgtcctg gtgacaggga tgatggtgaa cgatcgaagc tggcgctgta
     7981 tcaggaatcg ctgatcaagg ccgggcaacc gcacgcgttc gcgttacatg agccgtcgct
     8041 gtatcgctgg ggggcgtatc tgaagagccg ccgcgcccgg tatttacagg agacagacgc
     8101 aggtgttgct gcggcctcag acgcgcttga tcggcatgct ttcgggcgtg ctattggtgc
     8161 ggtcctgggg gctgatgatg agtgagtttg ccgtctcgcg tctggtcatc cctgaggtgg
     8221 tgatccctga cggggttgtt gctgcgcgaa aggccagggg gatcgcgcaa cgtgtcgcag
     8281 ctgctcgccg tgctgctgaa cgtcgtgctg cgcctacatg gtgctgggtg agacggggcg
     8341 cgttatgcgc cctttttttc gcccttcgcc tggcgtgcct tgcgccggaa tgccttgatc
     8401 tctgtcaggc gggctttggc gttttcggcc tcctccgggg ttgctggttc ggtggggttg
     8461 ccgtccaggt cgtagcgcat gcactggtcg atgaggctct gctggtacga gctcgtaccc
     8521 gtgacaatgc ccaggatgcg acggacggct ttgcggtctg cctctggcgg catgttcagc
     8581 tctgcgcctc gggtcaggat ctgctcgatg atgccctttg ccatggggcg acggtcaact
     8641 ccccgggtca gcaagtcagg ccagtgctct ttcaggaatg ctttggtcac ccggttggcc
     8701 agcgcctttt tggacttgtt ggcgatcgcg atgtcctgct ggcggcggtc gttctcctcc
     8761 tgtctgcgga tcccctcggc gacctgttca ggcgtctttt tcggctttgc cgctgtgggc
     8821 ttctgtgcgg gcttctgcgg ggctttcgct tttggctgta ccttcgcctt gctggtagtc
     8881 cgttcggctg gctgcggctc agatttcgcg gccttttccg gcgggacgta gaccgtgcgg
     8941 ttgcgtcgct tgatggtcag gatcggacgg gtggtttcgg tcataaatct ctctctggtc
     9001 ggttggtccg gatgtggtcg gcaagggcga tcagggtggc ggcgtcgccg cggttccgat
     9061 cgatgcgccg gatgatgtcg ttgatgtagt cccagcgccc gacggcggca aggacggaaa
     9121 gggcgaggcg gttgaaccgg ctctctggta cccggtcgag ggtcaggggg atgcgcttgc
     9181 gggggcgtcc tggaccgcgc cctgtccctg gtcttttgag cagctgcggc tgctccccct
     9241 gcggcaaaat aacggggatc ttcgccaggc tgcgcgtctc gttctgcgcg aactcgggca
     9301 ccgcgatgtg ctcgtctgtc catgtcctgc ctccgggcct ggctgatgcg ctggagtcgc
     9361 aggacgggca ggagacgacg atctcccgtc gcggcttgcc cttgaccggt gccttgctga
     9421 tcaccagggg cgcggctttg caatcagcgc agaccgacga tgtccagacc gcgttgtctg
     9481 gcagggtggc gttccggtac tggtgcgcgg cgtcatgcgg tgtctggaag ctgaccgtct
     9541 gaaacgtggg ggcgacgctg agaggtgccg cccatggctg gcacctggga caagcaagct
     9601 ccacctctcc ggcgtgcccg tcgccaccgt tccagcgcag gggctcgcgg tgttcggggc
     9661 agtagtacac gaaaccgtcg cggcgtgttt gcccgaccgg gcctggtcgg cgatcgtgga
     9721 accgtgcgcc cgggttggct cgcatggcgc ggacgagcgc ctcgccctgg tggatgccgt
     9781 accagacctg ccagccatcg ccgatgccga tcaggaacgg cgagacgaac ggtggggtgc
     9841 ggttgactct cccgcgtgcg ttggctccga tggtggcttc gcgttcgcgg ttccagcagg
     9901 agacgcacag gctcgaaccg ggcttattgg gggcgatcag gcggttgtct ttccggctgc
     9961 aacgggggca gagcttacgg gtgtctgccg gggtggtcag tggtgcgtca gggggctgtt
    10021 cggtaccgac ccaaagcgcg aggccagtgg ggcaccggtg gcactcgagg tgggggtttt
    10081 caatcgtgcg atcgaggtgg ctctgctcat aaatctgtcg gcatgcctcg acccggaggg
    10141 agcacccgcc ggggcaggtg acgaacaaaa cgccgggcat gatgtcgggg aaccgctccg
    10201 cagcgcctac gcacttcagt gcctcggttg acggctgcgt cgcttctgtc gtgtcgttat
    10261 cgtcgttcac tgctccccct gcgtcgaaat ggtcgccaga cctcgccagc actggctgtg
    10321 cgtctttttt agtatctcac aaaaaacgcc actttggcgt taattgtgtt aagcttgtct
    10381 tactgattcc tgacgagcag ttgggaaaca tgaaaccgga tcgcccggtg ccgctttccc
    10441 tcctccccaa cgaggcgggg ttgtaaacga ggatgcgcgc ttcgtccttg gcaggtgcga
    10501 gtaagcgaga agggctgctg tcctgccgct atgcggtaag tcctcaaggg cggctttatc
    10561 gataatcgtc actcccgccc cccgatgccc gttaagctga ggggggcacc tatctggtac
    10621 tcccaatgaa aaaacgaatc tataccgctg acctcgctgc gctgctgaac gtgtcgctgc
    10681 gcacgctctc ccgcctgagt tcgcggggga tcgttccgac tccggcaggg cgttcccttc
    10741 gtggcgggca ttactggctg gctggggatc ccgtgctcaa acgctttatt gccgaccagc
    10801 gtcgtcccgg gagggctcct tatggcgttc gataactctc acctgactga cgctggcctg
    10861 ttctggcgtg ccgtctctgc ggctggggct ccggttgacc gcgaggactg gctgcgcctc
    10921 tgttctggca tcgcatgctg ggcatctctc tgcggagagg acgggcttgc tgctatcgag
    10981 gcctggtcta cccgccacaa tcgcggatct gcgcatcacc ggtcgcagta ccggcacttc
    11041 ctgaagctga aaggcgtaac caatcccgag gccatggcgc aagtattcct gcggttctca
    11101 ggcgaggtgt actggcggct cgactcaaat cgcacgccgc cgtcgactga acaggttgct
    11161 gctcttgctg aacggaaacg ccgatcgatc gaggcggtgg ccagacgcga agatgccgac
    11221 cggcgcaagg ccgataaggc tgcggctgat gcggtcagga tgctggcgaa aggcgacggt
    11281 aatccggcct ataccccgta ctggcgcaat aagaccacaa tcgccaggcg tggtggcgtc
    11341 agcctcccgc ccctccccgc tgaccttcgt gtgggtggct tgttccgctc gttcgaaccg
    11401 agggcgtact ggtcgcgctg tctgcttgtt cctgcctatc gcctgaccga tgatcgccag
    11461 ctggttggcg acgcgctgga ggccatctgc ggcgtctgct ctgctggcgg caaaaccggc
    11521 gacaagttct cccctgcagg tacccaaaag gcgggacggt tctatccgat gcccggcttt
    11581 atggacgtgc tcgatacctc accacatgcg ccctactcgc ctctgttcgt ctgtgagggg
    11641 ttcgccacgg cggttgccct gcatcatctg acgaagggca aggtggcagt ctgcgcggcg
    11701 ttctcaattg ccgggttcgg cagctgcgtg gcgtcgctgc gccggttctg gccagatgtt
    11761 gatgtgatgc tggtaccgga tcacggcgaa gcgtaccgcc tggcgcataa agcggcctat
    11821 ggcgatgagc gagagccggt cccgacaatt ccaggcgcgg cggttgtgtc gttcgacggc
    11881 ctcgatcgct tcacgccccg ggataacctc gactggttcg acgtgctggt tgaggaaggg
    11941 gaagatcggg ctcgtgagct gctgcgctac gcggttcgtc atgcccggct tgagcggcgt
    12001 tgattggcgt gtccgaatgg tgcgcctttc gggacatctt ttttgccgtc gtggcgtctg
    12061 gtttagcgaa cgactttcga gacactaccg atgctttttt gatgagacgg actaaagcga
    12121 cgcaccggcg ctgccgtcgt gacgctatcc tgcgatcggt ttctcacagc agcataaagc
    12181 ccggcctcgt cttgatgtcg ggtttttttt tagcccaaag gagggcacat gaaatcaaag
    12241 actgaactac gcgaggccgc gacggctcgc tctctggcgg tgatcgccac tgaaatgtcg
    12301 gagtggtcgc tcgatgggtt ctcccacctc aagctgccga acttctccgc tggggaacgc
    12361 cagcagacgc tgtccgggtc ggtggtggtt gatcgcccgc cgttcgacta cgagtgggct
    12421 ggtaccgaga aatttaacac tctggccacc cgtgcgctgc aggtgaaact tccggcatcg
    12481 cgtgagcgta actacgcctg gttgtgcggc gttgaacggg agacgctggc gacggcgctc
    12541 ctcgtggagc tgttctccgt caccggctgc gtggcgttcg ccgggctggg taaggttgcc
    12601 gatctggcgt tcctgacgct ggacgagagc gaggcggggc agatcagggc ggcgatgctg
    12661 cagtggctcg atggggcggc ggcgtagggg ctcgatcctc gatcagctgg agtgggaagg
    12721 gcgactgatc gaggaggcca tggcgacgga actgcggggc gacccggtgc gctgcgacca
    12781 ccttgctgcg gcgcggttat ggctggagtg tgttgaccgg gcgtctgatg acaggtggcg
    12841 ggcgttctgc ctcgccagct atgaatggtg cgtattcgt
//
