LOCUS       NZ_OX291589             3223 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1048 plasmid 4, complete sequence.
ACCESSION   NZ_OX291589
VERSION     NZ_OX291589.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201015
            Assembly: GCF_946406635.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:39:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,243
            CDSs (total)                      :: 5,112
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 30 of 75
            Pseudo Genes (incomplete)         :: 44 of 75
            Pseudo Genes (internal stop)      :: 15 of 75
            Pseudo Genes (multiple problems)  :: 11 of 75
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291589.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1048"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="4"
     gene            606..929
                     /locus_tag="N4J61_RS26195"
                     /old_locus_tag="SAMEA13201015_05200"
     CDS             606..929
                     /locus_tag="N4J61_RS26195"
                     /old_locus_tag="SAMEA13201015_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1138..>1572
                     /locus_tag="N4J61_RS26200"
                     /pseudo=""
     CDS             1138..>1572
                     /locus_tag="N4J61_RS26200"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1607..2092
                     /locus_tag="N4J61_RS26205"
                     /old_locus_tag="SAMEA13201015_05201"
     CDS             1607..2092
                     /locus_tag="N4J61_RS26205"
                     /old_locus_tag="SAMEA13201015_05201"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            2096..2311
                     /locus_tag="N4J61_RS26210"
                     /old_locus_tag="SAMEA13201015_05202"
     CDS             2096..2311
                     /locus_tag="N4J61_RS26210"
                     /old_locus_tag="SAMEA13201015_05202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2479..2811
                     /locus_tag="N4J61_RS26215"
     CDS             2479..2811
                     /locus_tag="N4J61_RS26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     ARG             2480..2811
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          319..510
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.821"
                     /coverage="89.0"
                     /evalue="7.28e-73"
     Typing          complement(2..336)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.673"
                     /coverage="93.0"
                     /evalue="2.4e-81"
     Typing          complement(3021..3190)
                     /name="rep_cluster_2405"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.17e-84"
     Typing          920..2409
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accgagtttc aggcgtggaa tgagaaaccg cggccataac agcggaatga caccggtaaa
       61 ccgaaaggca ggaatgcggg ggagcacgag ggagccacca gggggaaacg cctggtatct
      121 ttaagccgca tcgggtttcg ccaccgctga tttgagcgtc attttcgtga tactcgtcag
      181 gggggcggag cctatggaaa aacggcaact ccgcatcctt ttcttggcct ttcctgcttc
      241 gagcggcatt tgcttatccc gtaatctgtg agctggtaac gcccgccgca gtctcacggc
      301 aggagcgtag cgaccgagtg agcgaggagg cgtcatttca tcaccacctg cattttgcac
      361 ttacgcccgg cagccgcatt tttcccctgt cacatgaagc actttcataa attaccactc
      421 cgtcataaca tgtttagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
      481 cgccccgaaa cccgctaaaa ccactgccgc gtctgcggct tgtccaacac cgtgccgacc
      541 gggaaaagat ttcccgtccg tcccggcgtt atcaggaggc cggattggca gacaaacgca
      601 gcaaaatgct caccatgtgg gtgactgagg acgagcaccg gcgtctgctg gagcgctgcg
      661 agggtaagca actcgcggcc tggatgcggc agacgtgcct ggacgagaag cccgcacgcg
      721 ccggcaaact tccgtcgatc tcgccggcgc tgcttcgtca gcttgccggc atggggaaca
      781 accttaacca gattgcccgc caggttaacg ccggtggtgg aagcggacac gaccgcgtgc
      841 agattgtcgc cgcgctgatg gccatcgatg ccggactcga gcggttgcgg catgccgtac
      901 tggaaaaggg tgctgatgat gatcgttaag tttcatcctc gggggcgcgg cggcggcggc
      961 gggccggtcg attatctgct gggtaaagat cgccagcgcg acggcgccag cgttctgcag
     1021 gggaagccgg atgaagtccg ggagcttatc gacgcctcgc cctacgccaa aaagtacact
     1081 tccggcgttc tgtcttttgc cgaacaggat ttaccgcccg gccagcgcga aaagctgatg
     1141 gcgagtttcg agcgggttct gatgcccgga ctcgataaag accagtacag cgtgctgtgg
     1201 gttgagcacc gggacaaggg gcggctggag ctgaacttcc tgatcccgaa cacggagctt
     1261 ctgaccggca agcggctaca gccgtattac gaccgcgccg accgtccgcg catcgatgcc
     1321 tggcagacca tagtgaacgg caggctgggg ctgcatgacc cgaacgcgcc ggagaaccgg
     1381 cgggtgctgg tctcgccttc cgcgctgccg gaagcgaagc aggaagccgc ccaggcgatt
     1441 acgagcggcc tgcttgccct tgcctcatcc ggggagctga aaacgcgtca ggacgtcact
     1501 gaggcgctgg aaagcgcagg ttttgaggtg gtgcgcacca caaaaagcag catcagcatt
     1561 gccgacccgg acggggggcg aaacatccga ctgaagggag ccatctatga acagtctttt
     1621 aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagacta ccggcgagat
     1681 gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcga
     1741 gaagagaatc agcgccgcca tcctcgacca cgaccggaag ctgtcctcag ccatgagcca
     1801 gcgcacgaaa gggatgctgc gcatggtcag ccagacgtgg ctgaccatcg ttctggtctc
     1861 cgcgctgctg atagcgtcga gcgcgggcat tctgtggtgg caggggcagc agatactcga
     1921 gaactatacg accatccggg agcagaagag cacgcaggcc atgctgtcag agaggaacag
     1981 cggcgtacag ctctcgacct gcggcgagca gagacgccgc tgcgtgaggg tgaacccgga
     2041 agcgggacag ttcggagagg actcgagctg gatgatactg gcggggaaat agcacatgac
     2101 ggagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
     2161 gctggacgag tgggagaacg ccttcgcgga atggcggacg atgtctggtc ttattcaacg
     2221 ggagaacgcg gcgctgaacg agcgcgtcac ggtcttgagc aggcaggtgc agagtttgag
     2281 cgagcagctg cgccggttgt cgaaaggctg aacgcgatag aaactcaccg gcagcaggag
     2341 cgggcggtgc agcaccagaa agcgctggag ctggaacgat cgcagtggca gcatcatggt
     2401 ccttcgctat gataagccgg gttctattaa ttagttgtta tgtaaagata tttaaatttt
     2461 tcaacatgag gtatttttat gaatacatat attttcctgg gaggggctat tgtggctgaa
     2521 gtcataggca ccacctttat gaaatattca gaaggattta cacgcctgtg gccgtctgta
     2581 ggaactgcaa taagttattg cgctgccttt tatctgcttt cccaaacatt ggaaaatata
     2641 cctaccggta ttgcttacgc aatttggtct ggtgcgggaa ttgtactaat aagcctagct
     2701 ggttggttgg ttagcggtca aaaacttgat tggcccgcaa ttgcaggaat gttccttatt
     2761 tgcgttggag ttttggttat taatattttt tcaaaatctt ctgcgcatta actttttacc
     2821 gaagcatatt ggccaaagca aaaggatttc cttgagatcc tttctttctg cgcgtaatct
     2881 ttttccctgt aaacaaaaaa accaccgcta ccaacggtgg ttcgtttgcc ggaattcaga
     2941 gctaccaact ctttgttccg atggtaactg gctttcacgg agcgcagata cctaaactgt
     3001 cctttcagtg tagccgcact caggctttca cttcaagaac tctgtaggca ttctctacaa
     3061 tctctgctcc gtgaacccag ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3121 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3181 gttcgtgcat acagtccatc ctggagcgaa ctgcctaccc gga
//
