LOCUS       NZ_OX291587            12879 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1048 plasmid 2, complete sequence.
ACCESSION   NZ_OX291587
VERSION     NZ_OX291587.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201015
            Assembly: GCF_946406635.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:39:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,243
            CDSs (total)                      :: 5,112
            Genes (coding)                    :: 5,038
            CDSs (with protein)               :: 5,038
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 30 of 75
            Pseudo Genes (incomplete)         :: 44 of 75
            Pseudo Genes (internal stop)      :: 15 of 75
            Pseudo Genes (multiple problems)  :: 11 of 75
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291587.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1048"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="2"
     gene            join(12770..12879,1..247)
                     /locus_tag="N4J61_RS26090"
     CDS             join(12770..12879,1..247)
                     /locus_tag="N4J61_RS26090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559062.1"
                     /translation="MPRHLQGKTRMPLAGGCYSAGHRLALISLLPVLEARPGDRDDGER
                     SKLALYQESLIKAGQPHAFALHEPSLYRWGAYLKSRRARYLQETDAGVAAASDALDRHA
                     FGRAIGAVLGADDE"
                     /name="hypothetical protein"
     gene            complement(405..1046)
                     /locus_tag="N4J61_RS26095"
                     /old_locus_tag="SAMEA13201015_05185"
     CDS             complement(405..1046)
                     /locus_tag="N4J61_RS26095"
                     /old_locus_tag="SAMEA13201015_05185"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_070559060.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELNMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1043..2332)
                     /locus_tag="N4J61_RS26100"
                     /old_locus_tag="SAMEA13201015_05186"
     CDS             complement(1043..2332)
                     /locus_tag="N4J61_RS26100"
                     /old_locus_tag="SAMEA13201015_05186"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157698538.1"
                     /translation="MNDDNDTTEATQPSTEALKCVGAAERFPDIMPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQGEQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRNRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            2688..2897
                     /locus_tag="N4J61_RS26105"
     CDS             2688..2897
                     /locus_tag="N4J61_RS26105"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765954.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPVLKRFIADQRRPGRAPYGVR"
                     /name="hypothetical protein"
     gene            2884..4065
                     /locus_tag="N4J61_RS26110"
                     /old_locus_tag="SAMEA13201015_05187"
     CDS             2884..4065
                     /locus_tag="N4J61_RS26110"
                     /old_locus_tag="SAMEA13201015_05187"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765953.1"
                     /translation="MAFDNSHLTDAGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSTRHNRGSAHHRSQYRHFLKLKGVTNPEAMAQVFLRFSGEVYWRLDSNR
                     TPPSTEQVAALAERKRRSIEAVARREDADRRKADKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDTSPHAPYSPLFVCEGFATAVALH
                     HLTKGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPV
                     PTIPGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            4291..4749
                     /locus_tag="N4J61_RS26115"
                     /old_locus_tag="SAMEA13201015_05188"
     CDS             4291..4749
                     /locus_tag="N4J61_RS26115"
                     /old_locus_tag="SAMEA13201015_05188"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765952.1"
                     /translation="MKSKTELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNTLATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDGAAA"
                     /name="hypothetical protein"
     gene            complement(5004..5588)
                     /locus_tag="N4J61_RS26120"
                     /old_locus_tag="SAMEA13201015_05189"
     CDS             complement(5004..5588)
                     /locus_tag="N4J61_RS26120"
                     /old_locus_tag="SAMEA13201015_05189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351424.1"
                     /translation="MDITISDATYSRLAAQAKGFETPEQVINRLIDVVEIDHDTRPELF
                     FNPEGEADFKKALLKSKQAKVEMFKRDGSCETGVWNARAISEESSVRANIWSGYLRGWK
                     EKGIVRAVFTVQEVPDLPGAAESVQWNDYTISRTFSGGISVQRNGMPVTIVKPFLRDVA
                     HDLKISLLNSNGNEMNTRQLGAAIIKKLNNS"
                     /name="hypothetical protein"
     gene            complement(6035..6526)
                     /locus_tag="N4J61_RS26125"
     CDS             complement(6035..6526)
                     /locus_tag="N4J61_RS26125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(6591..7082)
                     /locus_tag="N4J61_RS26130"
     CDS             complement(6591..7082)
                     /locus_tag="N4J61_RS26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558888.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGT
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQTAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            7610..8905
                     /locus_tag="N4J61_RS26135"
                     /old_locus_tag="SAMEA13201015_05191"
     CDS             7610..8905
                     /locus_tag="N4J61_RS26135"
                     /old_locus_tag="SAMEA13201015_05191"
                     /inference="COORDINATES: protein motif:HMM:NF017116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_119879886.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKLDVDDCDPTAFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            complement(9551..10018)
                     /locus_tag="N4J61_RS26140"
                     /old_locus_tag="SAMEA13201015_05192"
     CDS             complement(9551..10018)
                     /locus_tag="N4J61_RS26140"
                     /old_locus_tag="SAMEA13201015_05192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_070559148.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATAATVARTMGIPPRTVRDWAKART
                     DAPSNVLSIEEMRERAQRAVEATPQAAIRRLKNHFVQRQFELLQRHATDLQALRTASLQ
                     AMLEKDATMVKAISGLMTSLLKTQERERVIYEIKPGTEADIMREGMNRQQS"
                     /name="transposase"
     gene            complement(10217..10462)
                     /locus_tag="N4J61_RS26145"
                     /old_locus_tag="SAMEA13201015_05193"
     CDS             complement(10217..10462)
                     /locus_tag="N4J61_RS26145"
                     /old_locus_tag="SAMEA13201015_05193"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558916.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTVANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(10581..10766)
                     /locus_tag="N4J61_RS26150"
                     /old_locus_tag="SAMEA13201015_05194"
     CDS             complement(10581..10766)
                     /locus_tag="N4J61_RS26150"
                     /old_locus_tag="SAMEA13201015_05194"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879885.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDSA
                     GRYQTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            complement(10877..11464)
                     /locus_tag="N4J61_RS26155"
                     /old_locus_tag="SAMEA13201015_05195"
     CDS             complement(10877..11464)
                     /locus_tag="N4J61_RS26155"
                     /old_locus_tag="SAMEA13201015_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181654175.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQILQEGDTLVIYAIDRIGRNARDALELVEELEKRKVTLVSIREGVDLS
                     SAVGQAVLGIMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            11724..12380
                     /locus_tag="N4J61_RS26160"
                     /old_locus_tag="SAMEA13201015_05196"
     CDS             11724..12380
                     /locus_tag="N4J61_RS26160"
                     /old_locus_tag="SAMEA13201015_05196"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879883.1"
                     /translation="MTEIKTECPDATWLRTTLAEMASDHFPVYDLPCLSVDPNSSAQLT
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLRTEAERLRGLIADGKTALRAGETVSPD
                     AGVAAFLLPDIEAELVAAEAAEADVARERDALLQDADRRDAAAAIGLFNLAHAVRVQRI
                     EQLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEA"
                     /name="hypothetical protein"
     gene            12381..12764
                     /locus_tag="N4J61_RS26165"
                     /old_locus_tag="SAMEA13201015_05197"
     CDS             12381..12764
                     /locus_tag="N4J61_RS26165"
                     /old_locus_tag="SAMEA13201015_05197"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559064.1"
                     /translation="MSAFSMLCDRLRHEATLAKSTGAGDALAQVLETGGAAQTFAPIGA
                     PVASPTESVHRARAQSIQDAAFLTEMKQAGVAPADLVMLDGVLASSRGLPDASRAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
ORIGIN
        1 tggtgacagg gatgatggtg aacgatcgaa gctggcgctg tatcaggaat cgctgatcaa
       61 ggccgggcaa ccgcacgcgt tcgcgttaca tgagccgtcg ctgtatcgct ggggggcgta
      121 tctgaagagc cgccgcgccc ggtatttaca ggagacagac gcaggtgttg ctgcggcctc
      181 agacgcgctt gatcggcatg ctttcgggcg tgctattggt gcggtcctgg gggctgatga
      241 tgagtgagtt tgccgtctcg cgtctggtca tccctgaggt ggtgatccct gacggggttg
      301 ttgctgcgcg aaaggccagg gggatcgcgc aacgtgtcgc agctgctcgc cgtgctgctg
      361 aacgtcgtgc tgcgcctaca tggtgctggg tgagacgggg cgcgttatgc gccctttttt
      421 tcgcccttcg cctggcgtgc cttgcgccgg aatgccttga tctctgtcag gcgggctttg
      481 gcgttttcgg cctcctccgg ggttgctggt tcggtggggt tgccgtccag gtcgtagcgc
      541 atgcactggt cgatgaggct ctgctggtac gagctcgtac ccgtgacaat gcccaggatg
      601 cgacggacgg ctttgcggtc tgcctctggc ggcatgttca gctctgcgcc tcgggtcagg
      661 atctgctcga tgatgccctt tgccatgggg cgacggtcaa ctccccgggt cagcaagtca
      721 ggccagtgct ctttcaggaa tgctttggtc acccggttgg ccagcgcctt tttggacttg
      781 ttggcgatcg cgatgtcctg ctggcggcgg tcgttctcct cctgtctgcg gatcccctcg
      841 gcgacctgtt caggcgtctt tttcggcttt gccgctgtgg gcttctgtgc gggcttctgc
      901 ggggctttcg cttttggctg taccttcgcc ttgctggtag tccgttcggc tggctgcggc
      961 tcagatttcg cggccttttc cggcgggacg tagaccgtgc ggttgcgtcg cttgatggtc
     1021 aggatcggac gggtggtttc ggtcataaat ctctctctgg tcggttggtc cggatgtggt
     1081 cggcaagggc gatcagggtg gcggcgtcgc cgcggttccg atcgatgcgc cggatgatgt
     1141 cgttgatgta gtcccagcgc ccgacggcgg caaggacgga aagggcgagg cggttgaacc
     1201 ggctctctgg tacccggtcg agggtcaggg ggatgcgctt gcgggggcgt cctggaccgc
     1261 gccctgtccc tggtcttttg agcagctgcg gctgctcccc ctgcggcaaa ataacgggga
     1321 tcttcgccag gctgcgcgtc tcgttctgcg cgaactcggg caccgcgatg tgctcgtctg
     1381 tccatgtcct gcctccgggc ctggctgatg cgctggagtc gcaggacggg caggagacga
     1441 cgatctcccg tcgcggcttg cccttgaccg gtgccttgct gatcaccagg ggcgcggctt
     1501 tgcaatcagc gcagaccgac gatgtccaga ccgcgttgtc tggcagggtg gcgttccggt
     1561 actggtgcgc ggcgtcatgc ggtgtctgga agctgaccgt ctgaaacgtg ggggcgacgc
     1621 tgagaggtgc cgcccatggc tggcacctgg gacaagcaag ctccacctct ccggcgtgcc
     1681 cgtcgccacc gttccagcgc aggggctcgc ggtgttcggg gcagtagtac acgaaaccgt
     1741 cgcggcgtgt ttgcccgacc gggcctggtc ggcgatcgtg gaaccgtgcg cccgggttgg
     1801 ctcgcatggc gcggacgagc gcctcgccct ggtggatgcc gtaccagacc tgccagccat
     1861 cgccgatgcc gatcaggaac ggcgagacga acggtggggt gcggttgact ctcccgcgtg
     1921 cgttggctcc gatggtggct tcgcgttcgc ggttccagca ggagacgcac aggctcgaac
     1981 cgggcttatt gggggcgatc aggcggttgt ctttccggct gcaacggggg cagagcttac
     2041 gggtgtctgc cggggtggtc agtggtgcgt cagggggctg ttcggtaccg acccaaagcg
     2101 cgaggccagt ggggcaccgg tggcactcga ggtgggggtt ttcaatcgtg cgatcgaggt
     2161 ggctctgctc ataaatctgt cggcatgcct cgacccggag ggagcacccg ccggggcagg
     2221 tgacgaacaa aacgccgggc atgatgtcgg ggaaccgctc cgcagcgcct acgcacttca
     2281 gtgcctcggt tgacggctgc gtcgcttctg tcgtgtcgtt atcgtcgttc actgctcccc
     2341 ctgcgtcgaa atggtcgcca gacctcgcca gcactggctg tgcgtctttt ttagtatctc
     2401 acaaaaaacg ccactttggc gttaattgtg ttaagcttgt cttactgatt cctgacgagc
     2461 agttgggaaa catgaaaccg gatcgcccgg tgccgctttc cctcctcccc aacgaggcgg
     2521 ggttgtaaac gaggatgcgc gcttcgtcct tggcaggtgc gagtaagcga gaagggctgc
     2581 tgtcctgccg ctatgcggta agtcctcaag ggcggcttta tcgataatcg tcactcccgc
     2641 cccccgatgc ccgttaagct gaggggggca cctatctggt actcccaatg aaaaaacgaa
     2701 tctataccgc tgacctcgct gcgctgctga acgtgtcgct gcgcacgctc tcccgcctga
     2761 gttcgcgggg gatcgttccg actccggcag ggcgttccct tcgtggcggg cattactggc
     2821 tggctgggga tcccgtgctc aaacgcttta ttgccgacca gcgtcgtccc gggagggctc
     2881 cttatggcgt tcgataactc tcacctgact gacgctggcc tgttctggcg tgccgtctct
     2941 gcggctgggg ctccggttga ccgcgaggac tggctgcgcc tctgttctgg catcgcatgc
     3001 tgggcatctc tctgcggaga ggacgggctt gctgctatcg aggcctggtc tacccgccac
     3061 aatcgcggat ctgcgcatca ccggtcgcag taccggcact tcctgaagct gaaaggcgta
     3121 accaatcccg aggccatggc gcaagtattc ctgcggttct caggcgaggt gtactggcgg
     3181 ctcgactcaa atcgcacgcc gccgtcgact gaacaggttg ctgctcttgc tgaacggaaa
     3241 cgccgatcga tcgaggcggt ggccagacgc gaagatgccg accggcgcaa ggccgataag
     3301 gctgcggctg atgcggtcag gatgctggcg aaaggcgacg gtaatccggc ctataccccg
     3361 tactggcgca ataagaccac aatcgccagg cgtggtggcg tcagcctccc gcccctcccc
     3421 gctgaccttc gtgtgggtgg cttgttccgc tcgttcgaac cgagggcgta ctggtcgcgc
     3481 tgtctgcttg ttcctgccta tcgcctgacc gatgatcgcc agctggttgg cgacgcgctg
     3541 gaggccatct gcggcgtctg ctctgctggc ggcaaaaccg gcgacaagtt ctcccctgca
     3601 ggtacccaaa aggcgggacg gttctatccg atgcccggct ttatggacgt gctcgatacc
     3661 tcaccacatg cgccctactc gcctctgttc gtctgtgagg ggttcgccac ggcggttgcc
     3721 ctgcatcatc tgacgaaggg caaggtggca gtctgcgcgg cgttctcaat tgccgggttc
     3781 ggcagctgcg tggcgtcgct gcgccggttc tggccagatg ttgatgtgat gctggtaccg
     3841 gatcacggcg aagcgtaccg cctggcgcat aaagcggcct atggcgatga gcgagagccg
     3901 gtcccgacaa ttccaggcgc ggcggttgtg tcgttcgacg gcctcgatcg cttcacgccc
     3961 cgggataacc tcgactggtt cgacgtgctg gttgaggaag gggaagatcg ggctcgtgag
     4021 ctgctgcgct acgcggttcg tcatgcccgg cttgagcggc gttgattggc gtgtccgaat
     4081 ggtgcgcctt tcgggacatc ttttttgccg tcgtggcgtc tggtttagcg aacgactttc
     4141 gagacactac cgatgctttt ttgatgagac ggactaaagc gacgcaccgg cgctgccgtc
     4201 gtgacgctat cctgcgatcg gtttctcaca gcagcataaa gcccggcctc gtcttgatgt
     4261 cgggtttttt tttagcccaa aggagggcac atgaaatcaa agactgaact acgcgaggcc
     4321 gcgacggctc gctctctggc ggtgatcgcc actgaaatgt cggagtggtc gctcgatggg
     4381 ttctcccacc tcaagctgcc gaacttctcc gctggggaac gccagcagac gctgtccggg
     4441 tcggtggtgg ttgatcgccc gccgttcgac tacgagtggg ctggtaccga gaaatttaac
     4501 actctggcca cccgtgcgct gcaggtgaaa cttccggcat cgcgtgagcg taactacgcc
     4561 tggttgtgcg gcgttgaacg ggagacgctg gcgacggcgc tcctcgtgga gctgttctcc
     4621 gtcaccggct gcgtggcgtt cgccgggctg ggtaaggttg ccgatctggc gttcctgacg
     4681 ctggacgaga gcgaggcggg gcagatcagg gcggcgatgc tgcagtggct cgatggggcg
     4741 gcggcgtagg ggctcgatcc tcgatcagct ggagtgggaa gggcgactga tcgaggaggc
     4801 catggcgacg gaactgcggg gcgacccggt gcgctgcgac caccttgctg cggcgcggtt
     4861 atggctggag tgtgttgacc gggcgtctga tgacaggtgg cgggcgttct gcctcgccag
     4921 ctatgaatgg tgcgtattcg tcgcagtaga actctgaccg gcggcggcgt tgagccgtcg
     4981 ctcctctgcg tgaagcatcg atgttaagag ttatttaact tctttatgat cgctgcgcca
     5041 agctgccgag tattcatttc atttccattg ctatttagca atgatatttt taaatcatgc
     5101 gcaacatcac gtagaaatgg cttcacaata gttacaggca tgccatttct ttgaactgaa
     5161 ataccgccac taaaagtgcg actgattgta taatcattcc actgcacact ttcagccgct
     5221 cctggcaagt caggaacctc ctggacagtg aaaaccgcac gaacaatccc tttttccttc
     5281 catccacgca aatacccact ccaaatattt gcccgaactg atgattcttc tgaaatcgca
     5341 cgagcattcc atactccagt ttcacaggaa ccatcccttt taaacatttc gactttggcc
     5401 tgttttgatt ttagtaacgc ttttttaaaa tccgcttcac cttcaggatt aaaaaaaagt
     5461 tcagggcgtg tgtcatgatc gatttcaaca acatcgatca gacggtttat tacctgttcg
     5521 ggagtttcaa atccctttgc ttgtgcagca agacggctat atgtagcgtc agatatcgta
     5581 atatccatca aagtacctct atagatcaaa aattaatcat aaaattaatc ataattgata
     5641 aatttaatga tttcaagatc tataattacc tgatatcgca atatggttaa ccgcctctaa
     5701 agagctataa aaacctgtaa acccgctcct cgtgccgctt ggttataaac aaaccagttg
     5761 aatctacgtc aacaatagcc tactattgtt gtactgcaag agcattcgca gggggtatta
     5821 accagtaatt aaccctttgc gctgggcagt caggaggctg gtctcctgtt gggaagttat
     5881 gttgcggctt cctagtaata acagtgaatt tgatagacag cagaaaaccg ccgatgctgc
     5941 tagatccgaa aggaaccaat gaagggaaat atttaggcgt tatggttcat tctcattaaa
     6001 ttgaggagaa ttgcttaaat gggcgtcctt cctatcaccc tcgctgtata gctttacatt
     6061 tggcctccgt tgcatggtat gcagtcacgg gggccaaatt attttcagag acatcaacta
     6121 tcgccattgc gaaaaagcca gccccagcaa cgttaatgcg acgaacagca ttgagcacaa
     6181 tagtctgctc cgagtaatct ctgttaacgt acacattgct ctgcactgag aatgcagttg
     6241 ccaaaatctc cgcacactct tgccaactta gcttatcctt cgagttacga gcagatagca
     6301 catgcctaaa cattgcccaa gtgatcggta ttgctggtga tgcaggtatg tcccagccgt
     6361 ttctctgaca aacaactttc atttgattga actgcttctt agtcatcaga taggtaaaac
     6421 aaggcgtctc ccaaacttcc tgagggagac ctttatcatc catttcttca gcttgctgtg
     6481 taaaagcata gaaagcgtca cggatagcat catcaccgat agccacttct tttccttgta
     6541 aaatttttat gttcgaattt tatcatattt agctatgtca cttaggactg ctaattcagt
     6601 agcccacttt cccgcaactt atggcgatag tcccggacac ggctggtgtt ccactcctgt
     6661 cccgtcgctg tccggtgccc tgctgcgttc agtgccgacg cgatggcggg cagagtcgcg
     6721 ttctctgcgg tcatgctgac aatgagctgg tcagcctcat cactgacggc ctggaagcgc
     6781 ggatcggctc cccgggggac tatctgtctt gcaggttgtt gtgcgctttg tggtgctgtt
     6841 tgttgtgcgg tttgtcgtgc aggtttaccg ccgacggaca gcgccctgac ttcagccctc
     6901 agcgcttcga tggcagctaa aagtttggtg tccatccctt ccggcttcgt gccaagtccg
     6961 gcgttcagcg cctctatgac agcatccgtg cgggttgctc cggttcgtgc tgcgttctgc
     7021 tcgatgcgtt cgatcaggct aacgggtagg cgaacggtca gggttttggt tttctctctc
     7081 atggctgctg ctctccacgt cgtgcgtttt gtcgtgctgt ttgtcttgcg tagtgtaatg
     7141 cgttttgtcg tgcgcgtcga attcgacgtc gtgcagttgt cgtgcacaga ggggcgacgg
     7201 ctttgcgtcg ccggggtgcc gtttgtgtac atggataaat agcagaggcg gcgcagagat
     7261 tgagctgggc gtgatcacga cagcgcgata agtgcgcggc gctaagtggc gtggagggtt
     7321 gggatcgggt aagctgatct ggcgtctgcg gatccggttg gggatcttcg ggcacaaaaa
     7381 aaggcgacct gttgggtggt cacctttttc tgccggggga gctggtaact cctccgatta
     7441 attcgagtca cgtcttagcg ggacgttctc gaaggaagaa tagcacttgc gcagcgtact
     7501 cctcaaactc aaagagaagt gtaccacgtc gataagatcg cgcaagtgtg aatttccctc
     7561 gtaagccccc gcctgacgcc gactcctacg tttttaaagg agtaattgcg tgtcacaaat
     7621 tgatgaaaat tccggcactg ctgccgacaa cgtaacgccg atcgccgctg ccaaggtggg
     7681 acgcccgtct ctacaaaaaa aatacccgac taccgcgttt cccgtggagc tggagatcaa
     7741 ctaccggaag gggcgctact cgtcctctct tgagaacgtt gtcgccgcta tgcgctgcca
     7801 ttccgtcacc ggcatccagc tcgctttcga tgagttcttt gcggaggagg gggcgttcgt
     7861 gaacgggcaa tggtacccgc tgtccgacaa cctagttacc gagctgcgtc tgcgcctcgc
     7921 tgctgccgac ttcgagaagg tgaccaagga caatgcccgc gacgccatgt tctacgtctg
     7981 cgatgagaac aaattcgaca gcgcccagcg ttggctgacg gctcaggaat gggacggcaa
     8041 acctcgcgtc agttacttct tcgagcggtg cttcaaatgc caaggggatc ccgagtacct
     8101 ggcggcggtc agcctctaca cctggacggc gcttgccggg cgcgtaatga atcccggaat
     8161 caaggccgac atggttccgc tgctgtacgg gcgacagggt gcccgcaaat cgacgggcgt
     8221 tcgccttatg tcgccattcc ctgactccta ctccagcttc aacctgaacg acaaggacga
     8281 ctccctagtg cgccgtatgc gtgggtgcct gattggcgag ctggaggagc tgcgcggcct
     8341 ccacgggcgc gactccgaat cggtgaaagc gttcttctcc gctgcccacg acgagtgggt
     8401 gccgaaattt aaagagttca aggtgcgaaa cccgcgccgc gtggtgttca tcggtaccac
     8461 gaacaacaag cagatcctca atgactcgac ggggaaccgc cgctatttgc ccatgaattc
     8521 cggggacgtg gactgcgcgg ccatcgagaa aaaccgctcg cagctgtggg ctgaagcgct
     8581 ccacctgttc aatgagaatg gggtgatgtg ggaggccgct gaaacgctgg cgcgtaaggt
     8641 gcatgacgag ttccgcgtcg aggatccgtg ggaagacgag atcgccgagc tgctcaagct
     8701 ggacgttgac gactgcgacc cgactgcttt cgtgccgacg aaggccttgt tctctcaggt
     8761 agggctcatg attggccata ccagcaacaa gcagcatcag cgcatggcgg ctatcatgtc
     8821 ccgattgggc tatgaggttg gcaggaagcg cataaacggt gcgttagttc gtggctactt
     8881 ccgtcgaacg caagctgtcg tgtaagggtt acttttccgt aagaggggag ctatatgctc
     8941 ccctttttta ttgttccacc cgggtggatc acgggtggat catcacgccg tttttgtacc
     9001 acctgttcca cccgggtgtt ccacccgttc cacccgtgtt ccgcccgtgg atggaacagc
     9061 taaggcctcg tgtttaaagg ctttcattgt tgtgttccac ccgttccacc ctattttcta
     9121 attaaaaaca aaaaatgaat aaattgggta aatagggggt aaatgctcac taattgaaca
     9181 attagttgtt ttttttacta tttatgaatc tatgccggat cgggtggaac gggtggatca
     9241 tcttggaatc gacgcgccgc agctgcaccc tgccggatgc actttgcttg tcttattaga
     9301 tcggtggatg ggttcgtcgt gccgggatgg tcggtggtag tggttctgag ggggatcgga
     9361 ggtttgggtg gcgctgaggg cacgaggctt tcgcctcgcg ctgggtggtc gtctgttggt
     9421 tctgcgtgag ggcttgccct cgcgcacgcg agcggtaaca actcgttaac gtgcggatcc
     9481 gcgtatttcg cgggctcccg ggttttctgc ggcggtaaag cggcggtaaa aggcggtttt
     9541 cccgccgttt tcagctctgt tggcgattca tgccctcgcg catgatgtcg gcctcggtgc
     9601 ctggcttgat ctcgtagatg acgcgctcgc gttcctgcgt cttgagtagg gatgtcataa
     9661 ggccgctgat cgctttgacc atggtggcgt ccttctccag catggcctgt agcgaagctg
     9721 tgcggagggc ttgcaggtcg gtggcgtggc gctgtagcag ctcaaactgg cgctgtacaa
     9781 agtggttttt taggcgtcgg atcgctgcct gtggggtggc ctcgacggct cgctgtgcgc
     9841 gttcgcgcat ctcctcgatg ctgaggacgt tgctgggtgc gtcggtgcgg gctttcgccc
     9901 aatctctgac ggtgcggggt gggatcccca tggtgcgtgc gacggtggct gcggttgcgc
     9961 cgtcctgctc cagtagtctg agcgcctggg ctttctgctc ggtggtgtat ttcttcatgg
    10021 tgtcctgctc cggttggttt ctctcctgta ttgaaacgtc acgcgctgaa aatggcgggg
    10081 gtttttgggg ggtacacggc gtttgtgcgt gcgtggtcgt gcggttgggt atttttgtgg
    10141 ctgaggcgct tagaatggct ctggtgcgtt ctgggtaagg gaggaggcgc agcagtcgct
    10201 gcgctcctag ctggggttag cgccagcgtt cgaggcggta cctgacggtg atcccgtcct
    10261 ccgtcatggt atggatgtac tcctggtttg ccacggtgcg gtcgatggcc ttggtgcggt
    10321 gctttttgta cgttttcatc ctggtttctt cggagaagtt ctcgccggta accatctcgt
    10381 cccatgactc atatgacttt gctccgggaa ccatgattac cttccatctc tggaacctac
    10441 ggggaatgtt ttcaaatgcc atcgttctct ccttatgatt ctggtttctg ggggcgctcc
    10501 cgcgcccctg taagtggttc gtaatggcgt aaggcttttg ttattgcttg ctgcttacac
    10561 ctgtaaggct ttcgttatgg tcatgcttcc caggcgtaaa ggctgatgat gctgactgtc
    10621 tggtaccgac cggcgctgtc gcggagggtg gtctggtact ggcctttgcg gttggcggcg
    10681 caatggcggg gcttgccgaa ctggcggatc tcgccgtgtt caatgctcag gccgggatag
    10741 cgcggtgttg ctgcttggtt tatcatggtt ctgttccttg ttgggaatgg tgcccgggtc
    10801 tgctcctcat gcgttccccg ggctgggttg cggcggtgtg gaatggttgg agccacctac
    10861 accgctgcgc tctctcttac tccggcgcta ccgcgtcgac cttctcaagg tggcggtaaa
    10921 tcgcgctcct gctcagattt aactgcttgg ctatggtggc tattggtacc cccatggcgc
    10981 ggcgcatgac tatctccccc gcttctttcg cgccgatctt ggtcgggcgt ccgaacttct
    11041 ggccacggct ctttgcggcc tcgatccctg ccgcctggcg ttccagtatg agcttgcgct
    11101 ccatctgcgc gaaactcgcc atgatgccga ggacggcctg tccgacggcg ctgctcaaat
    11161 cgacaccctc gcggatgctg accagggtta cttttcgttt ctccagctcc tcgaccagtt
    11221 cgagggcgtc ccgggcgttc ctgccgatgc ggtcgatcgc gtagatgacc agcgtgtcgc
    11281 cctcctgcag gatctggttc aggaggatgc cgagggctgg acgctcgcgt ggtggtaccg
    11341 tcccgctgat cccgtcgtcg gcgaggatgg aggctgttgg gaaccgggcg atgatggcgt
    11401 cgcgctggct gttgctggtt tgttcgtcgg ttgatacgcg aacgtaaccg tatgttttgc
    11461 tcactttttt ctctcctctg accgatacgt ctctttcgac acaacatgcc acaactgtac
    11521 cgaaagtcta tcgctaaagt gtctatttcg tcgctttgtc gggtgtcccg gaatgtaggg
    11581 gattagcgac ggcctccagg cggctctccg cgctgggcga gagccctgcg ccctctgcgt
    11641 tgatgtctgc gcggtgtcgt gacggcatgc tgtctgagcg gcgtttgctg ccgctttgct
    11701 gataacttgc tgagaaccaa accatgaccg aaatcaaaac cgaatgcccg gacgcgacct
    11761 ggctgcgtac cacgcttgct gaaatggcga gtgaccattt tcccgtttat gaccttcctt
    11821 gcctgtcggt ggatcctaac tcctccgctc aacttaccgc gcttgacgat cgccgggtgg
    11881 cgcgtgaaat gcgtcaggca gcagccgacg tggaaacgcg acgcattgac gccgctcgcg
    11941 tggtggaagg gctcaggacg gaagctgaac gcctgcgcgg gctgattgcc gacggcaaga
    12001 cggcgctgcg tgctggcgag actgtgagcc ccgatgcggg cgtcgctgcg ttcctgctgc
    12061 cggatatcga ggccgagctg gttgccgccg aggctgctga ggctgatgtg gcccgggagc
    12121 gtgacgcgct gctgcaggat gctgaccgcc gtgatgcggc ggctgcgatc ggcctgttca
    12181 acctggcgca cgccgtgcgc gtacagcgta ttgagcagct gctgaagctg gcgatggatg
    12241 aggcgacgct gcttgccgag tccgacgcgg ggcgcagttt gtaccgtact gttatcaatc
    12301 ctgatcgccg cctgaaccag ctgttctcca cgcagggtgc gatcgaaatt ctgcgccgca
    12361 atcgcggcat ggaggcgtaa atgtctgcat tttcgatgct gtgtgaccgg cttcgccatg
    12421 aggcgacgct ggcaaaatct accggggccg gcgacgcgct ggcgcaggtg ctggagacgg
    12481 gcggtgctgc gcagacgttc gcgccgatcg gggctccggt tgcctctcct acggagtcgg
    12541 ttcaccgggc gcgtgctcag tccatccagg atgcggcgtt cctgactgag atgaaacagg
    12601 cgggcgtggc tcctgccgat ctggtgatgc ttgacggcgt gctggcctcc tctcgcggac
    12661 tccctgatgc gtcgcgtgct ccggcgttcg ctgcggtgcg tgctcaggtg cagcaacagg
    12721 cgtctctctc ccctgctgcc cggggtatca attgggacga gtaaagccga tgccgcgcca
    12781 cctgcagggt aagacgcgga tgccgctggc aggtggttgt tactctgctg gccaccgtct
    12841 tgcgctgatc tcgttgctgc ctgttctgga ggctcgtcc
//
