LOCUS       NZ_OX291535            12879 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1046 plasmid 4, complete sequence.
ACCESSION   NZ_OX291535
VERSION     NZ_OX291535.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201012
            Assembly: GCF_946406225.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:59:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,261
            Genes (coding)                    :: 5,185
            CDSs (with protein)               :: 5,185
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 31 of 77
            Pseudo Genes (incomplete)         :: 49 of 77
            Pseudo Genes (internal stop)      :: 13 of 77
            Pseudo Genes (multiple problems)  :: 13 of 77
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291535.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1046"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="4"
     gene            complement(join(12788..12879,1..154))
                     /locus_tag="N4J72_RS26885"
     CDS             complement(join(12788..12879,1..154))
                     /locus_tag="N4J72_RS26885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558916.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTVANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            complement(273..458)
                     /locus_tag="N4J72_RS26890"
                     /old_locus_tag="SAMEA13201012_05350"
     CDS             complement(273..458)
                     /locus_tag="N4J72_RS26890"
                     /old_locus_tag="SAMEA13201012_05350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879885.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDSA
                     GRYQTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            complement(569..1156)
                     /locus_tag="N4J72_RS26895"
                     /old_locus_tag="SAMEA13201012_05351"
     CDS             complement(569..1156)
                     /locus_tag="N4J72_RS26895"
                     /old_locus_tag="SAMEA13201012_05351"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181654175.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQILQEGDTLVIYAIDRIGRNARDALELVEELEKRKVTLVSIREGVDLS
                     SAVGQAVLGIMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            1416..2072
                     /locus_tag="N4J72_RS26900"
                     /old_locus_tag="SAMEA13201012_05352"
     CDS             1416..2072
                     /locus_tag="N4J72_RS26900"
                     /old_locus_tag="SAMEA13201012_05352"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879883.1"
                     /translation="MTEIKTECPDATWLRTTLAEMASDHFPVYDLPCLSVDPNSSAQLT
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLRTEAERLRGLIADGKTALRAGETVSPD
                     AGVAAFLLPDIEAELVAAEAAEADVARERDALLQDADRRDAAAAIGLFNLAHAVRVQRI
                     EQLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEA"
                     /name="hypothetical protein"
     gene            2073..2456
                     /locus_tag="N4J72_RS26905"
                     /old_locus_tag="SAMEA13201012_05353"
     CDS             2073..2456
                     /locus_tag="N4J72_RS26905"
                     /old_locus_tag="SAMEA13201012_05353"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559064.1"
                     /translation="MSAFSMLCDRLRHEATLAKSTGAGDALAQVLETGGAAQTFAPIGA
                     PVASPTESVHRARAQSIQDAAFLTEMKQAGVAPADLVMLDGVLASSRGLPDASRAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            2462..2818
                     /locus_tag="N4J72_RS26910"
     CDS             2462..2818
                     /locus_tag="N4J72_RS26910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559062.1"
                     /translation="MPRHLQGKTRMPLAGGCYSAGHRLALISLLPVLEARPGDRDDGER
                     SKLALYQESLIKAGQPHAFALHEPSLYRWGAYLKSRRARYLQETDAGVAAASDALDRHA
                     FGRAIGAVLGADDE"
                     /name="hypothetical protein"
     gene            complement(2976..3617)
                     /locus_tag="N4J72_RS26915"
                     /old_locus_tag="SAMEA13201012_05354"
     CDS             complement(2976..3617)
                     /locus_tag="N4J72_RS26915"
                     /old_locus_tag="SAMEA13201012_05354"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_070559060.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELNMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(3614..4903)
                     /locus_tag="N4J72_RS26920"
                     /old_locus_tag="SAMEA13201012_05355"
     CDS             complement(3614..4903)
                     /locus_tag="N4J72_RS26920"
                     /old_locus_tag="SAMEA13201012_05355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157698538.1"
                     /translation="MNDDNDTTEATQPSTEALKCVGAAERFPDIMPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQGEQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRNRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            5259..5468
                     /locus_tag="N4J72_RS26925"
     CDS             5259..5468
                     /locus_tag="N4J72_RS26925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765954.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPVLKRFIADQRRPGRAPYGVR"
                     /name="hypothetical protein"
     gene            5455..6636
                     /locus_tag="N4J72_RS26930"
                     /old_locus_tag="SAMEA13201012_05356"
     CDS             5455..6636
                     /locus_tag="N4J72_RS26930"
                     /old_locus_tag="SAMEA13201012_05356"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765953.1"
                     /translation="MAFDNSHLTDAGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSTRHNRGSAHHRSQYRHFLKLKGVTNPEAMAQVFLRFSGEVYWRLDSNR
                     TPPSTEQVAALAERKRRSIEAVARREDADRRKADKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDTSPHAPYSPLFVCEGFATAVALH
                     HLTKGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPV
                     PTIPGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            6862..7320
                     /locus_tag="N4J72_RS26935"
                     /old_locus_tag="SAMEA13201012_05357"
     CDS             6862..7320
                     /locus_tag="N4J72_RS26935"
                     /old_locus_tag="SAMEA13201012_05357"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765952.1"
                     /translation="MKSKTELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNTLATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDGAAA"
                     /name="hypothetical protein"
     gene            complement(7575..8159)
                     /locus_tag="N4J72_RS26940"
                     /old_locus_tag="SAMEA13201012_05358"
     CDS             complement(7575..8159)
                     /locus_tag="N4J72_RS26940"
                     /old_locus_tag="SAMEA13201012_05358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351424.1"
                     /translation="MDITISDATYSRLAAQAKGFETPEQVINRLIDVVEIDHDTRPELF
                     FNPEGEADFKKALLKSKQAKVEMFKRDGSCETGVWNARAISEESSVRANIWSGYLRGWK
                     EKGIVRAVFTVQEVPDLPGAAESVQWNDYTISRTFSGGISVQRNGMPVTIVKPFLRDVA
                     HDLKISLLNSNGNEMNTRQLGAAIIKKLNNS"
                     /name="hypothetical protein"
     gene            complement(8606..9097)
                     /locus_tag="N4J72_RS26945"
     CDS             complement(8606..9097)
                     /locus_tag="N4J72_RS26945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            complement(9162..9653)
                     /locus_tag="N4J72_RS26950"
     CDS             complement(9162..9653)
                     /locus_tag="N4J72_RS26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558888.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGT
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQTAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            10181..11476
                     /locus_tag="N4J72_RS26955"
                     /old_locus_tag="SAMEA13201012_05360"
     CDS             10181..11476
                     /locus_tag="N4J72_RS26955"
                     /old_locus_tag="SAMEA13201012_05360"
                     /inference="COORDINATES: protein motif:HMM:NF017116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_119879886.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKLDVDDCDPTAFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            complement(12122..12589)
                     /locus_tag="N4J72_RS26960"
                     /old_locus_tag="SAMEA13201012_05361"
     CDS             complement(12122..12589)
                     /locus_tag="N4J72_RS26960"
                     /old_locus_tag="SAMEA13201012_05361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_070559148.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATAATVARTMGIPPRTVRDWAKART
                     DAPSNVLSIEEMRERAQRAVEATPQAAIRRLKNHFVQRQFELLQRHATDLQALRTASLQ
                     AMLEKDATMVKAISGLMTSLLKTQERERVIYEIKPGTEADIMREGMNRQQS"
                     /name="transposase"
ORIGIN
        1 ccttggtgcg gtgctttttg tacgttttca tcctggtttc ttcggagaag ttctcgccgg
       61 taaccatctc gtcccatgac tcatatgact ttgctccggg aaccatgatt accttccatc
      121 tctggaacct acggggaatg ttttcaaatg ccatcgttct ctccttatga ttctggtttc
      181 tgggggcgct cccgcgcccc tgtaagtggt tcgtaatggc gtaaggcttt tgttattgct
      241 tgctgcttac acctgtaagg ctttcgttat ggtcatgctt cccaggcgta aaggctgatg
      301 atgctgactg tctggtaccg accggcgctg tcgcggaggg tggtctggta ctggcctttg
      361 cggttggcgg cgcaatggcg gggcttgccg aactggcgga tctcgccgtg ttcaatgctc
      421 aggccgggat agcgcggtgt tgctgcttgg tttatcatgg ttctgttcct tgttgggaat
      481 ggtgcccggg tctgctcctc atgcgttccc cgggctgggt tgcggcggtg tggaatggtt
      541 ggagccacct acaccgctgc gctctctctt actccggcgc taccgcgtcg accttctcaa
      601 ggtggcggta aatcgcgctc ctgctcagat ttaactgctt ggctatggtg gctattggta
      661 cccccatggc gcggcgcatg actatctccc ccgcttcttt cgcgccgatc ttggtcgggc
      721 gtccgaactt ctggccacgg ctctttgcgg cctcgatccc tgccgcctgg cgttccagta
      781 tgagcttgcg ctccatctgc gcgaaactcg ccatgatgcc gaggacggcc tgtccgacgg
      841 cgctgctcaa atcgacaccc tcgcggatgc tgaccagggt tacttttcgt ttctccagct
      901 cctcgaccag ttcgagggcg tcccgggcgt tcctgccgat gcggtcgatc gcgtagatga
      961 ccagcgtgtc gccctcctgc aggatctggt tcaggaggat gccgagggct ggacgctcgc
     1021 gtggtggtac cgtcccgctg atcccgtcgt cggcgaggat ggaggctgtt gggaaccggg
     1081 cgatgatggc gtcgcgctgg ctgttgctgg tttgttcgtc ggttgatacg cgaacgtaac
     1141 cgtatgtttt gctcactttt ttctctcctc tgaccgatac gtctctttcg acacaacatg
     1201 ccacaactgt accgaaagtc tatcgctaaa gtgtctattt cgtcgctttg tcgggtgtcc
     1261 cggaatgtag gggattagcg acggcctcca ggcggctctc cgcgctgggc gagagccctg
     1321 cgccctctgc gttgatgtct gcgcggtgtc gtgacggcat gctgtctgag cggcgtttgc
     1381 tgccgctttg ctgataactt gctgagaacc aaaccatgac cgaaatcaaa accgaatgcc
     1441 cggacgcgac ctggctgcgt accacgcttg ctgaaatggc gagtgaccat tttcccgttt
     1501 atgaccttcc ttgcctgtcg gtggatccta actcctccgc tcaacttacc gcgcttgacg
     1561 atcgccgggt ggcgcgtgaa atgcgtcagg cagcagccga cgtggaaacg cgacgcattg
     1621 acgccgctcg cgtggtggaa gggctcagga cggaagctga acgcctgcgc gggctgattg
     1681 ccgacggcaa gacggcgctg cgtgctggcg agactgtgag ccccgatgcg ggcgtcgctg
     1741 cgttcctgct gccggatatc gaggccgagc tggttgccgc cgaggctgct gaggctgatg
     1801 tggcccggga gcgtgacgcg ctgctgcagg atgctgaccg ccgtgatgcg gcggctgcga
     1861 tcggcctgtt caacctggcg cacgccgtgc gcgtacagcg tattgagcag ctgctgaagc
     1921 tggcgatgga tgaggcgacg ctgcttgccg agtccgacgc ggggcgcagt ttgtaccgta
     1981 ctgttatcaa tcctgatcgc cgcctgaacc agctgttctc cacgcagggt gcgatcgaaa
     2041 ttctgcgccg caatcgcggc atggaggcgt aaatgtctgc attttcgatg ctgtgtgacc
     2101 ggcttcgcca tgaggcgacg ctggcaaaat ctaccggggc cggcgacgcg ctggcgcagg
     2161 tgctggagac gggcggtgct gcgcagacgt tcgcgccgat cggggctccg gttgcctctc
     2221 ctacggagtc ggttcaccgg gcgcgtgctc agtccatcca ggatgcggcg ttcctgactg
     2281 agatgaaaca ggcgggcgtg gctcctgccg atctggtgat gcttgacggc gtgctggcct
     2341 cctctcgcgg actccctgat gcgtcgcgtg ctccggcgtt cgctgcggtg cgtgctcagg
     2401 tgcagcaaca ggcgtctctc tcccctgctg cccggggtat caattgggac gagtaaagcc
     2461 gatgccgcgc cacctgcagg gtaagacgcg gatgccgctg gcaggtggtt gttactctgc
     2521 tggccaccgt cttgcgctga tctcgttgct gcctgttctg gaggctcgtc ctggtgacag
     2581 ggatgatggt gaacgatcga agctggcgct gtatcaggaa tcgctgatca aggccgggca
     2641 accgcacgcg ttcgcgttac atgagccgtc gctgtatcgc tggggggcgt atctgaagag
     2701 ccgccgcgcc cggtatttac aggagacaga cgcaggtgtt gctgcggcct cagacgcgct
     2761 tgatcggcat gctttcgggc gtgctattgg tgcggtcctg ggggctgatg atgagtgagt
     2821 ttgccgtctc gcgtctggtc atccctgagg tggtgatccc tgacggggtt gttgctgcgc
     2881 gaaaggccag ggggatcgcg caacgtgtcg cagctgctcg ccgtgctgct gaacgtcgtg
     2941 ctgcgcctac atggtgctgg gtgagacggg gcgcgttatg cgcccttttt ttcgcccttc
     3001 gcctggcgtg ccttgcgccg gaatgccttg atctctgtca ggcgggcttt ggcgttttcg
     3061 gcctcctccg gggttgctgg ttcggtgggg ttgccgtcca ggtcgtagcg catgcactgg
     3121 tcgatgaggc tctgctggta cgagctcgta cccgtgacaa tgcccaggat gcgacggacg
     3181 gctttgcggt ctgcctctgg cggcatgttc agctctgcgc ctcgggtcag gatctgctcg
     3241 atgatgccct ttgccatggg gcgacggtca actccccggg tcagcaagtc aggccagtgc
     3301 tctttcagga atgctttggt cacccggttg gccagcgcct ttttggactt gttggcgatc
     3361 gcgatgtcct gctggcggcg gtcgttctcc tcctgtctgc ggatcccctc ggcgacctgt
     3421 tcaggcgtct ttttcggctt tgccgctgtg ggcttctgtg cgggcttctg cggggctttc
     3481 gcttttggct gtaccttcgc cttgctggta gtccgttcgg ctggctgcgg ctcagatttc
     3541 gcggcctttt ccggcgggac gtagaccgtg cggttgcgtc gcttgatggt caggatcgga
     3601 cgggtggttt cggtcataaa tctctctctg gtcggttggt ccggatgtgg tcggcaaggg
     3661 cgatcagggt ggcggcgtcg ccgcggttcc gatcgatgcg ccggatgatg tcgttgatgt
     3721 agtcccagcg cccgacggcg gcaaggacgg aaagggcgag gcggttgaac cggctctctg
     3781 gtacccggtc gagggtcagg gggatgcgct tgcgggggcg tcctggaccg cgccctgtcc
     3841 ctggtctttt gagcagctgc ggctgctccc cctgcggcaa aataacgggg atcttcgcca
     3901 ggctgcgcgt ctcgttctgc gcgaactcgg gcaccgcgat gtgctcgtct gtccatgtcc
     3961 tgcctccggg cctggctgat gcgctggagt cgcaggacgg gcaggagacg acgatctccc
     4021 gtcgcggctt gcccttgacc ggtgccttgc tgatcaccag gggcgcggct ttgcaatcag
     4081 cgcagaccga cgatgtccag accgcgttgt ctggcagggt ggcgttccgg tactggtgcg
     4141 cggcgtcatg cggtgtctgg aagctgaccg tctgaaacgt gggggcgacg ctgagaggtg
     4201 ccgcccatgg ctggcacctg ggacaagcaa gctccacctc tccggcgtgc ccgtcgccac
     4261 cgttccagcg caggggctcg cggtgttcgg ggcagtagta cacgaaaccg tcgcggcgtg
     4321 tttgcccgac cgggcctggt cggcgatcgt ggaaccgtgc gcccgggttg gctcgcatgg
     4381 cgcggacgag cgcctcgccc tggtggatgc cgtaccagac ctgccagcca tcgccgatgc
     4441 cgatcaggaa cggcgagacg aacggtgggg tgcggttgac tctcccgcgt gcgttggctc
     4501 cgatggtggc ttcgcgttcg cggttccagc aggagacgca caggctcgaa ccgggcttat
     4561 tgggggcgat caggcggttg tctttccggc tgcaacgggg gcagagctta cgggtgtctg
     4621 ccggggtggt cagtggtgcg tcagggggct gttcggtacc gacccaaagc gcgaggccag
     4681 tggggcaccg gtggcactcg aggtgggggt tttcaatcgt gcgatcgagg tggctctgct
     4741 cataaatctg tcggcatgcc tcgacccgga gggagcaccc gccggggcag gtgacgaaca
     4801 aaacgccggg catgatgtcg gggaaccgct ccgcagcgcc tacgcacttc agtgcctcgg
     4861 ttgacggctg cgtcgcttct gtcgtgtcgt tatcgtcgtt cactgctccc cctgcgtcga
     4921 aatggtcgcc agacctcgcc agcactggct gtgcgtcttt tttagtatct cacaaaaaac
     4981 gccactttgg cgttaattgt gttaagcttg tcttactgat tcctgacgag cagttgggaa
     5041 acatgaaacc ggatcgcccg gtgccgcttt ccctcctccc caacgaggcg gggttgtaaa
     5101 cgaggatgcg cgcttcgtcc ttggcaggtg cgagtaagcg agaagggctg ctgtcctgcc
     5161 gctatgcggt aagtcctcaa gggcggcttt atcgataatc gtcactcccg ccccccgatg
     5221 cccgttaagc tgaggggggc acctatctgg tactcccaat gaaaaaacga atctataccg
     5281 ctgacctcgc tgcgctgctg aacgtgtcgc tgcgcacgct ctcccgcctg agttcgcggg
     5341 ggatcgttcc gactccggca gggcgttccc ttcgtggcgg gcattactgg ctggctgggg
     5401 atcccgtgct caaacgcttt attgccgacc agcgtcgtcc cgggagggct ccttatggcg
     5461 ttcgataact ctcacctgac tgacgctggc ctgttctggc gtgccgtctc tgcggctggg
     5521 gctccggttg accgcgagga ctggctgcgc ctctgttctg gcatcgcatg ctgggcatct
     5581 ctctgcggag aggacgggct tgctgctatc gaggcctggt ctacccgcca caatcgcgga
     5641 tctgcgcatc accggtcgca gtaccggcac ttcctgaagc tgaaaggcgt aaccaatccc
     5701 gaggccatgg cgcaagtatt cctgcggttc tcaggcgagg tgtactggcg gctcgactca
     5761 aatcgcacgc cgccgtcgac tgaacaggtt gctgctcttg ctgaacggaa acgccgatcg
     5821 atcgaggcgg tggccagacg cgaagatgcc gaccggcgca aggccgataa ggctgcggct
     5881 gatgcggtca ggatgctggc gaaaggcgac ggtaatccgg cctatacccc gtactggcgc
     5941 aataagacca caatcgccag gcgtggtggc gtcagcctcc cgcccctccc cgctgacctt
     6001 cgtgtgggtg gcttgttccg ctcgttcgaa ccgagggcgt actggtcgcg ctgtctgctt
     6061 gttcctgcct atcgcctgac cgatgatcgc cagctggttg gcgacgcgct ggaggccatc
     6121 tgcggcgtct gctctgctgg cggcaaaacc ggcgacaagt tctcccctgc aggtacccaa
     6181 aaggcgggac ggttctatcc gatgcccggc tttatggacg tgctcgatac ctcaccacat
     6241 gcgccctact cgcctctgtt cgtctgtgag gggttcgcca cggcggttgc cctgcatcat
     6301 ctgacgaagg gcaaggtggc agtctgcgcg gcgttctcaa ttgccgggtt cggcagctgc
     6361 gtggcgtcgc tgcgccggtt ctggccagat gttgatgtga tgctggtacc ggatcacggc
     6421 gaagcgtacc gcctggcgca taaagcggcc tatggcgatg agcgagagcc ggtcccgaca
     6481 attccaggcg cggcggttgt gtcgttcgac ggcctcgatc gcttcacgcc ccgggataac
     6541 ctcgactggt tcgacgtgct ggttgaggaa ggggaagatc gggctcgtga gctgctgcgc
     6601 tacgcggttc gtcatgcccg gcttgagcgg cgttgattgg cgtgtccgaa tggtgcgcct
     6661 ttcgggacat cttttttgcc gtcgtggcgt ctggtttagc gaacgacttt cgagacacta
     6721 ccgatgcttt tttgatgaga cggactaaag cgacgcaccg gcgctgccgt cgtgacgcta
     6781 tcctgcgatc ggtttctcac agcagcataa agcccggcct cgtcttgatg tcgggttttt
     6841 ttttagccca aaggagggca catgaaatca aagactgaac tacgcgaggc cgcgacggct
     6901 cgctctctgg cggtgatcgc cactgaaatg tcggagtggt cgctcgatgg gttctcccac
     6961 ctcaagctgc cgaacttctc cgctggggaa cgccagcaga cgctgtccgg gtcggtggtg
     7021 gttgatcgcc cgccgttcga ctacgagtgg gctggtaccg agaaatttaa cactctggcc
     7081 acccgtgcgc tgcaggtgaa acttccggca tcgcgtgagc gtaactacgc ctggttgtgc
     7141 ggcgttgaac gggagacgct ggcgacggcg ctcctcgtgg agctgttctc cgtcaccggc
     7201 tgcgtggcgt tcgccgggct gggtaaggtt gccgatctgg cgttcctgac gctggacgag
     7261 agcgaggcgg ggcagatcag ggcggcgatg ctgcagtggc tcgatggggc ggcggcgtag
     7321 gggctcgatc ctcgatcagc tggagtggga agggcgactg atcgaggagg ccatggcgac
     7381 ggaactgcgg ggcgacccgg tgcgctgcga ccaccttgct gcggcgcggt tatggctgga
     7441 gtgtgttgac cgggcgtctg atgacaggtg gcgggcgttc tgcctcgcca gctatgaatg
     7501 gtgcgtattc gtcgcagtag aactctgacc ggcggcggcg ttgagccgtc gctcctctgc
     7561 gtgaagcatc gatgttaaga gttatttaac ttctttatga tcgctgcgcc aagctgccga
     7621 gtattcattt catttccatt gctatttagc aatgatattt ttaaatcatg cgcaacatca
     7681 cgtagaaatg gcttcacaat agttacaggc atgccatttc tttgaactga aataccgcca
     7741 ctaaaagtgc gactgattgt ataatcattc cactgcacac tttcagccgc tcctggcaag
     7801 tcaggaacct cctggacagt gaaaaccgca cgaacaatcc ctttttcctt ccatccacgc
     7861 aaatacccac tccaaatatt tgcccgaact gatgattctt ctgaaatcgc acgagcattc
     7921 catactccag tttcacagga accatccctt ttaaacattt cgactttggc ctgttttgat
     7981 tttagtaacg cttttttaaa atccgcttca ccttcaggat taaaaaaaag ttcagggcgt
     8041 gtgtcatgat cgatttcaac aacatcgatc agacggttta ttacctgttc gggagtttca
     8101 aatccctttg cttgtgcagc aagacggcta tatgtagcgt cagatatcgt aatatccatc
     8161 aaagtacctc tatagatcaa aaattaatca taaaattaat cataattgat aaatttaatg
     8221 atttcaagat ctataattac ctgatatcgc aatatggtta accgcctcta aagagctata
     8281 aaaacctgta aacccgctcc tcgtgccgct tggttataaa caaaccagtt gaatctacgt
     8341 caacaatagc ctactattgt tgtactgcaa gagcattcgc agggggtatt aaccagtaat
     8401 taaccctttg cgctgggcag tcaggaggct ggtctcctgt tgggaagtta tgttgcggct
     8461 tcctagtaat aacagtgaat ttgatagaca gcagaaaacc gccgatgctg ctagatccga
     8521 aaggaaccaa tgaagggaaa tatttaggcg ttatggttca ttctcattaa attgaggaga
     8581 attgcttaaa tgggcgtcct tcctatcacc ctcgctgtat agctttacat ttggcctccg
     8641 ttgcatggta tgcagtcacg ggggccaaat tattttcaga gacatcaact atcgccattg
     8701 cgaaaaagcc agccccagca acgttaatgc gacgaacagc attgagcaca atagtctgct
     8761 ccgagtaatc tctgttaacg tacacattgc tctgcactga gaatgcagtt gccaaaatct
     8821 ccgcacactc ttgccaactt agcttatcct tcgagttacg agcagatagc acatgcctaa
     8881 acattgccca agtgatcggt attgctggtg atgcaggtat gtcccagccg tttctctgac
     8941 aaacaacttt catttgattg aactgcttct tagtcatcag ataggtaaaa caaggcgtct
     9001 cccaaacttc ctgagggaga cctttatcat ccatttcttc agcttgctgt gtaaaagcat
     9061 agaaagcgtc acggatagca tcatcaccga tagccacttc ttttccttgt aaaattttta
     9121 tgttcgaatt ttatcatatt tagctatgtc acttaggact gctaattcag tagcccactt
     9181 tcccgcaact tatggcgata gtcccggaca cggctggtgt tccactcctg tcccgtcgct
     9241 gtccggtgcc ctgctgcgtt cagtgccgac gcgatggcgg gcagagtcgc gttctctgcg
     9301 gtcatgctga caatgagctg gtcagcctca tcactgacgg cctggaagcg cggatcggct
     9361 ccccggggga ctatctgtct tgcaggttgt tgtgcgcttt gtggtgctgt ttgttgtgcg
     9421 gtttgtcgtg caggtttacc gccgacggac agcgccctga cttcagccct cagcgcttcg
     9481 atggcagcta aaagtttggt gtccatccct tccggcttcg tgccaagtcc ggcgttcagc
     9541 gcctctatga cagcatccgt gcgggttgct ccggttcgtg ctgcgttctg ctcgatgcgt
     9601 tcgatcaggc taacgggtag gcgaacggtc agggttttgg ttttctctct catggctgct
     9661 gctctccacg tcgtgcgttt tgtcgtgctg tttgtcttgc gtagtgtaat gcgttttgtc
     9721 gtgcgcgtcg aattcgacgt cgtgcagttg tcgtgcacag aggggcgacg gctttgcgtc
     9781 gccggggtgc cgtttgtgta catggataaa tagcagaggc ggcgcagaga ttgagctggg
     9841 cgtgatcacg acagcgcgat aagtgcgcgg cgctaagtgg cgtggagggt tgggatcggg
     9901 taagctgatc tggcgtctgc ggatccggtt ggggatcttc gggcacaaaa aaaggcgacc
     9961 tgttgggtgg tcaccttttt ctgccggggg agctggtaac tcctccgatt aattcgagtc
    10021 acgtcttagc gggacgttct cgaaggaaga atagcacttg cgcagcgtac tcctcaaact
    10081 caaagagaag tgtaccacgt cgataagatc gcgcaagtgt gaatttccct cgtaagcccc
    10141 cgcctgacgc cgactcctac gtttttaaag gagtaattgc gtgtcacaaa ttgatgaaaa
    10201 ttccggcact gctgccgaca acgtaacgcc gatcgccgct gccaaggtgg gacgcccgtc
    10261 tctacaaaaa aaatacccga ctaccgcgtt tcccgtggag ctggagatca actaccggaa
    10321 ggggcgctac tcgtcctctc ttgagaacgt tgtcgccgct atgcgctgcc attccgtcac
    10381 cggcatccag ctcgctttcg atgagttctt tgcggaggag ggggcgttcg tgaacgggca
    10441 atggtacccg ctgtccgaca acctagttac cgagctgcgt ctgcgcctcg ctgctgccga
    10501 cttcgagaag gtgaccaagg acaatgcccg cgacgccatg ttctacgtct gcgatgagaa
    10561 caaattcgac agcgcccagc gttggctgac ggctcaggaa tgggacggca aacctcgcgt
    10621 cagttacttc ttcgagcggt gcttcaaatg ccaaggggat cccgagtacc tggcggcggt
    10681 cagcctctac acctggacgg cgcttgccgg gcgcgtaatg aatcccggaa tcaaggccga
    10741 catggttccg ctgctgtacg ggcgacaggg tgcccgcaaa tcgacgggcg ttcgccttat
    10801 gtcgccattc cctgactcct actccagctt caacctgaac gacaaggacg actccctagt
    10861 gcgccgtatg cgtgggtgcc tgattggcga gctggaggag ctgcgcggcc tccacgggcg
    10921 cgactccgaa tcggtgaaag cgttcttctc cgctgcccac gacgagtggg tgccgaaatt
    10981 taaagagttc aaggtgcgaa acccgcgccg cgtggtgttc atcggtacca cgaacaacaa
    11041 gcagatcctc aatgactcga cggggaaccg ccgctatttg cccatgaatt ccggggacgt
    11101 ggactgcgcg gccatcgaga aaaaccgctc gcagctgtgg gctgaagcgc tccacctgtt
    11161 caatgagaat ggggtgatgt gggaggccgc tgaaacgctg gcgcgtaagg tgcatgacga
    11221 gttccgcgtc gaggatccgt gggaagacga gatcgccgag ctgctcaagc tggacgttga
    11281 cgactgcgac ccgactgctt tcgtgccgac gaaggccttg ttctctcagg tagggctcat
    11341 gattggccat accagcaaca agcagcatca gcgcatggcg gctatcatgt cccgattggg
    11401 ctatgaggtt ggcaggaagc gcataaacgg tgcgttagtt cgtggctact tccgtcgaac
    11461 gcaagctgtc gtgtaagggt tacttttccg taagagggga gctatatgct cccctttttt
    11521 attgttccac ccgggtggat cacgggtgga tcatcacgcc gtttttgtac cacctgttcc
    11581 acccgggtgt tccacccgtt ccacccgtgt tccgcccgtg gatggaacag ctaaggcctc
    11641 gtgtttaaag gctttcattg ttgtgttcca cccgttccac cctattttct aattaaaaac
    11701 aaaaaatgaa taaattgggt aaataggggg taaatgctca ctaattgaac aattagttgt
    11761 tttttttact atttatgaat ctatgccgga tcgggtggaa cgggtggatc atcttggaat
    11821 cgacgcgccg cagctgcacc ctgccggatg cactttgctt gtcttattag atcggtggat
    11881 gggttcgtcg tgccgggatg gtcggtggta gtggttctga gggggatcgg aggtttgggt
    11941 ggcgctgagg gcacgaggct ttcgcctcgc gctgggtggt cgtctgttgg ttctgcgtga
    12001 gggcttgccc tcgcgcacgc gagcggtaac aactcgttaa cgtgcggatc cgcgtatttc
    12061 gcgggctccc gggttttctg cggcggtaaa gcggcggtaa aaggcggttt tcccgccgtt
    12121 ttcagctctg ttggcgattc atgccctcgc gcatgatgtc ggcctcggtg cctggcttga
    12181 tctcgtagat gacgcgctcg cgttcctgcg tcttgagtag ggatgtcata aggccgctga
    12241 tcgctttgac catggtggcg tccttctcca gcatggcctg tagcgaagct gtgcggaggg
    12301 cttgcaggtc ggtggcgtgg cgctgtagca gctcaaactg gcgctgtaca aagtggtttt
    12361 ttaggcgtcg gatcgctgcc tgtggggtgg cctcgacggc tcgctgtgcg cgttcgcgca
    12421 tctcctcgat gctgaggacg ttgctgggtg cgtcggtgcg ggctttcgcc caatctctga
    12481 cggtgcgggg tgggatcccc atggtgcgtg cgacggtggc tgcggttgcg ccgtcctgct
    12541 ccagtagtct gagcgcctgg gctttctgct cggtggtgta tttcttcatg gtgtcctgct
    12601 ccggttggtt tctctcctgt attgaaacgt cacgcgctga aaatggcggg ggtttttggg
    12661 gggtacacgg cgtttgtgcg tgcgtggtcg tgcggttggg tatttttgtg gctgaggcgc
    12721 ttagaatggc tctggtgcgt tctgggtaag ggaggaggcg cagcagtcgc tgcgctccta
    12781 gctggggtta gcgccagcgt tcgaggcggt acctgacggt gatcccgtcc tccgtcatgg
    12841 tatggatgta ctcctggttt gccacggtgc ggtcgatgg
//
