LOCUS       NZ_OX291529            12879 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Serratia marcescens strain SJC1054 plasmid 2, complete sequence.
ACCESSION   NZ_OX291529
VERSION     NZ_OX291529.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA13201022
            Assembly: GCF_946406125.1
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  CONSRTM   Pathogen Informatics
  TITLE     Direct Submission
  JOURNAL   Submitted (11-AUG-2022) WELLCOME SANGER INSTITUTE, Pathogen
            Informatics, Wellcome Sanger Institute, CB10 1SA, United Kingdom
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/02/2023 07:47:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,242
            CDSs (total)                      :: 5,111
            Genes (coding)                    :: 5,039
            CDSs (with protein)               :: 5,039
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 29 of 73
            Pseudo Genes (incomplete)         :: 43 of 73
            Pseudo Genes (internal stop)      :: 15 of 73
            Pseudo Genes (multiple problems)  :: 11 of 73
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX291529.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12879
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="SJC1054"
                     /serovar="NA"
                     /isolation_source="Bloodstream"
                     /db_xref="taxon:615"
                     /plasmid="2"
     gene            join(12300..12879,1..710)
                     /locus_tag="N4J62_RS26090"
     CDS             join(12300..12879,1..710)
                     /locus_tag="N4J62_RS26090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157698538.1"
                     /translation="MNDDNDTTEATQPSTEALKCVGAAERFPDIMPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCPEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTSSVCADCKAAP
                     LVISKAPVKGKPRREIVVSCPSCDSSASARPGGRTWTDEHIAVPEFAQNETRSLAKIPV
                     ILPQGEQPQLLKRPGTGRGPGRPRKRIPLTLDRVPESRFNRLALSVLAAVGRWDYINDI
                     IRRIDRNRGDAATLIALADHIRTNRPERDL"
                     /name="hypothetical protein"
     gene            707..1348
                     /locus_tag="N4J62_RS26095"
                     /old_locus_tag="SAMEA13201022_05186"
     CDS             707..1348
                     /locus_tag="N4J62_RS26095"
                     /old_locus_tag="SAMEA13201022_05186"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_070559060.1"
                     /translation="MTETTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQVAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELNMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1506..1862)
                     /locus_tag="N4J62_RS26100"
     CDS             complement(1506..1862)
                     /locus_tag="N4J62_RS26100"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559062.1"
                     /translation="MPRHLQGKTRMPLAGGCYSAGHRLALISLLPVLEARPGDRDDGER
                     SKLALYQESLIKAGQPHAFALHEPSLYRWGAYLKSRRARYLQETDAGVAAASDALDRHA
                     FGRAIGAVLGADDE"
                     /name="hypothetical protein"
     gene            complement(1868..2251)
                     /locus_tag="N4J62_RS26105"
                     /old_locus_tag="SAMEA13201022_05187"
     CDS             complement(1868..2251)
                     /locus_tag="N4J62_RS26105"
                     /old_locus_tag="SAMEA13201022_05187"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070559064.1"
                     /translation="MSAFSMLCDRLRHEATLAKSTGAGDALAQVLETGGAAQTFAPIGA
                     PVASPTESVHRARAQSIQDAAFLTEMKQAGVAPADLVMLDGVLASSRGLPDASRAPAFA
                     AVRAQVQQQASLSPAARGINWDE"
                     /name="hypothetical protein"
     gene            complement(2252..2908)
                     /locus_tag="N4J62_RS26110"
                     /old_locus_tag="SAMEA13201022_05188"
     CDS             complement(2252..2908)
                     /locus_tag="N4J62_RS26110"
                     /old_locus_tag="SAMEA13201022_05188"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879883.1"
                     /translation="MTEIKTECPDATWLRTTLAEMASDHFPVYDLPCLSVDPNSSAQLT
                     ALDDRRVAREMRQAAADVETRRIDAARVVEGLRTEAERLRGLIADGKTALRAGETVSPD
                     AGVAAFLLPDIEAELVAAEAAEADVARERDALLQDADRRDAAAAIGLFNLAHAVRVQRI
                     EQLLKLAMDEATLLAESDAGRSLYRTVINPDRRLNQLFSTQGAIEILRRNRGMEA"
                     /name="hypothetical protein"
     gene            3168..3755
                     /locus_tag="N4J62_RS26115"
                     /old_locus_tag="SAMEA13201022_05189"
     CDS             3168..3755
                     /locus_tag="N4J62_RS26115"
                     /old_locus_tag="SAMEA13201022_05189"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_181654175.1"
                     /translation="MSKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQILQEGDTLVIYAIDRIGRNARDALELVEELEKRKVTLVSIREGVDLS
                     SAVGQAVLGIMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            3866..4051
                     /locus_tag="N4J62_RS26120"
                     /old_locus_tag="SAMEA13201022_05190"
     CDS             3866..4051
                     /locus_tag="N4J62_RS26120"
                     /old_locus_tag="SAMEA13201022_05190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119879885.1"
                     /translation="MINQAATPRYPGLSIEHGEIRQFGKPRHCAANRKGQYQTTLRDSA
                     GRYQTVSIISLYAWEA"
                     /name="hypothetical protein"
     gene            4170..4415
                     /locus_tag="N4J62_RS26125"
                     /old_locus_tag="SAMEA13201022_05191"
     CDS             4170..4415
                     /locus_tag="N4J62_RS26125"
                     /old_locus_tag="SAMEA13201022_05191"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558916.1"
                     /translation="MAFENIPRRFQRWKVIMVPGAKSYESWDEMVTGENFSEETRMKTY
                     KKHRTKAIDRTVANQEYIHTMTEDGITVRYRLERWR"
                     /name="hypothetical protein"
     gene            4614..5081
                     /locus_tag="N4J62_RS26130"
                     /old_locus_tag="SAMEA13201022_05192"
     CDS             4614..5081
                     /locus_tag="N4J62_RS26130"
                     /old_locus_tag="SAMEA13201022_05192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_070559148.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATAATVARTMGIPPRTVRDWAKART
                     DAPSNVLSIEEMRERAQRAVEATPQAAIRRLKNHFVQRQFELLQRHATDLQALRTASLQ
                     AMLEKDATMVKAISGLMTSLLKTQERERVIYEIKPGTEADIMREGMNRQQS"
                     /name="transposase"
     gene            complement(5727..7022)
                     /locus_tag="N4J62_RS26135"
                     /old_locus_tag="SAMEA13201022_05193"
     CDS             complement(5727..7022)
                     /locus_tag="N4J62_RS26135"
                     /old_locus_tag="SAMEA13201022_05193"
                     /inference="COORDINATES: protein motif:HMM:NF017116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="WP_119879886.1"
                     /translation="MSQIDENSGTAADNVTPIAAAKVGRPSLQKKYPTTAFPVELEINY
                     RKGRYSSSLENVVAAMRCHSVTGIQLAFDEFFAEEGAFVNGQWYPLSDNLVTELRLRLA
                     AADFEKVTKDNARDAMFYVCDENKFDSAQRWLTAQEWDGKPRVSYFFERCFKCQGDPEY
                     LAAVSLYTWTALAGRVMNPGIKADMVPLLYGRQGARKSTGVRLMSPFPDSYSSFNLNDK
                     DDSLVRRMRGCLIGELEELRGLHGRDSESVKAFFSAAHDEWVPKFKEFKVRNPRRVVFI
                     GTTNNKQILNDSTGNRRYLPMNSGDVDCAAIEKNRSQLWAEALHLFNENGVMWEAAETL
                     ARKVHDEFRVEDPWEDEIAELLKLDVDDCDPTAFVPTKALFSQVGLMIGHTSNKQHQRM
                     AAIMSRLGYEVGRKRINGALVRGYFRRTQAVV"
                     /name="VapE domain-containing protein"
     gene            7550..8041
                     /locus_tag="N4J62_RS26140"
     CDS             7550..8041
                     /locus_tag="N4J62_RS26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_070558888.1"
                     /translation="MREKTKTLTVRLPVSLIERIEQNAARTGATRTDAVIEALNAGLGT
                     KPEGMDTKLLAAIEALRAEVRALSVGGKPARQTAQQTAPQSAQQPARQIVPRGADPRFQ
                     AVSDEADQLIVSMTAENATLPAIASALNAAGHRTATGQEWNTSRVRDYRHKLRESGLLN
                     "
                     /name="hypothetical protein"
     gene            8106..8597
                     /locus_tag="N4J62_RS26145"
     CDS             8106..8597
                     /locus_tag="N4J62_RS26145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351425.1"
                     /translation="MAIGDDAIRDAFYAFTQQAEEMDDKGLPQEVWETPCFTYLMTKKQ
                     FNQMKVVCQRNGWDIPASPAIPITWAMFRHVLSARNSKDKLSWQECAEILATAFSVQSN
                     VYVNRDYSEQTIVLNAVRRINVAGAGFFAMAIVDVSENNLAPVTAYHATEAKCKAIQRG
                     "
                     /name="hypothetical protein"
     gene            9044..9628
                     /locus_tag="N4J62_RS26150"
                     /old_locus_tag="SAMEA13201022_05195"
     CDS             9044..9628
                     /locus_tag="N4J62_RS26150"
                     /old_locus_tag="SAMEA13201022_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004589499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061351424.1"
                     /translation="MDITISDATYSRLAAQAKGFETPEQVINRLIDVVEIDHDTRPELF
                     FNPEGEADFKKALLKSKQAKVEMFKRDGSCETGVWNARAISEESSVRANIWSGYLRGWK
                     EKGIVRAVFTVQEVPDLPGAAESVQWNDYTISRTFSGGISVQRNGMPVTIVKPFLRDVA
                     HDLKISLLNSNGNEMNTRQLGAAIIKKLNNS"
                     /name="hypothetical protein"
     gene            complement(9883..10341)
                     /locus_tag="N4J62_RS26155"
                     /old_locus_tag="SAMEA13201022_05196"
     CDS             complement(9883..10341)
                     /locus_tag="N4J62_RS26155"
                     /old_locus_tag="SAMEA13201022_05196"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765952.1"
                     /translation="MKSKTELREAATARSLAVIATEMSEWSLDGFSHLKLPNFSAGERQ
                     QTLSGSVVVDRPPFDYEWAGTEKFNTLATRALQVKLPASRERNYAWLCGVERETLATAL
                     LVELFSVTGCVAFAGLGKVADLAFLTLDESEAGQIRAAMLQWLDGAAA"
                     /name="hypothetical protein"
     gene            complement(10567..11748)
                     /locus_tag="N4J62_RS26160"
                     /old_locus_tag="SAMEA13201022_05197"
     CDS             complement(10567..11748)
                     /locus_tag="N4J62_RS26160"
                     /old_locus_tag="SAMEA13201022_05197"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765953.1"
                     /translation="MAFDNSHLTDAGLFWRAVSAAGAPVDREDWLRLCSGIACWASLCG
                     EDGLAAIEAWSTRHNRGSAHHRSQYRHFLKLKGVTNPEAMAQVFLRFSGEVYWRLDSNR
                     TPPSTEQVAALAERKRRSIEAVARREDADRRKADKAAADAVRMLAKGDGNPAYTPYWRN
                     KTTIARRGGVSLPPLPADLRVGGLFRSFEPRAYWSRCLLVPAYRLTDDRQLVGDALEAI
                     CGVCSAGGKTGDKFSPAGTQKAGRFYPMPGFMDVLDTSPHAPYSPLFVCEGFATAVALH
                     HLTKGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPV
                     PTIPGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            complement(11735..11944)
                     /locus_tag="N4J62_RS26165"
     CDS             complement(11735..11944)
                     /locus_tag="N4J62_RS26165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_088765954.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGDPVLKRFIADQRRPGRAPYGVR"
                     /name="hypothetical protein"
ORIGIN
        1 aaacacgccg cgacggtttc gtgtactact gccccgaaca ccgcgagccc ctgcgctgga
       61 acggtggcga cgggcacgcc ggagaggtgg agcttgcttg tcccaggtgc cagccatggg
      121 cggcacctct cagcgtcgcc cccacgtttc agacggtcag cttccagaca ccgcatgacg
      181 ccgcgcacca gtaccggaac gccaccctgc cagacaacgc ggtctggaca tcgtcggtct
      241 gcgctgattg caaagccgcg cccctggtga tcagcaaggc accggtcaag ggcaagccgc
      301 gacgggagat cgtcgtctcc tgcccgtcct gcgactccag cgcatcagcc aggcccggag
      361 gcaggacatg gacagacgag cacatcgcgg tgcccgagtt cgcgcagaac gagacgcgca
      421 gcctggcgaa gatccccgtt attttgccgc agggggagca gccgcagctg ctcaaaagac
      481 cagggacagg gcgcggtcca ggacgccccc gcaagcgcat ccccctgacc ctcgaccggg
      541 taccagagag ccggttcaac cgcctcgccc tttccgtcct tgccgccgtc gggcgctggg
      601 actacatcaa cgacatcatc cggcgcatcg atcggaaccg cggcgacgcc gccaccctga
      661 tcgcccttgc cgaccacatc cggaccaacc gaccagagag agatttatga ccgaaaccac
      721 ccgtccgatc ctgaccatca agcgacgcaa ccgcacggtc tacgtcccgc cggaaaaggc
      781 cgcgaaatct gagccgcagc cagccgaacg gactaccagc aaggcgaagg tacagccaaa
      841 agcgaaagcc ccgcagaagc ccgcacagaa gcccacagcg gcaaagccga aaaagacgcc
      901 tgaacaggtc gccgagggga tccgcagaca ggaggagaac gaccgccgcc agcaggacat
      961 cgcgatcgcc aacaagtcca aaaaggcgct ggccaaccgg gtgaccaaag cattcctgaa
     1021 agagcactgg cctgacttgc tgacccgggg agttgaccgt cgccccatgg caaagggcat
     1081 catcgagcag atcctgaccc gaggcgcaga gctgaacatg ccgccagagg cagaccgcaa
     1141 agccgtccgt cgcatcctgg gcattgtcac gggtacgagc tcgtaccagc agagcctcat
     1201 cgaccagtgc atgcgctacg acctggacgg caaccccacc gaaccagcaa ccccggagga
     1261 ggccgaaaac gccaaagccc gcctgacaga gatcaaggca ttccggcgca aggcacgcca
     1321 ggcgaagggc gaaaaaaagg gcgcataacg cgccccgtct cacccagcac catgtaggcg
     1381 cagcacgacg ttcagcagca cggcgagcag ctgcgacacg ttgcgcgatc cccctggcct
     1441 ttcgcgcagc aacaaccccg tcagggatca ccacctcagg gatgaccaga cgcgagacgg
     1501 caaactcact catcatcagc ccccaggacc gcaccaatag cacgcccgaa agcatgccga
     1561 tcaagcgcgt ctgaggccgc agcaacacct gcgtctgtct cctgtaaata ccgggcgcgg
     1621 cggctcttca gatacgcccc ccagcgatac agcgacggct catgtaacgc gaacgcgtgc
     1681 ggttgcccgg ccttgatcag cgattcctga tacagcgcca gcttcgatcg ttcaccatca
     1741 tccctgtcac caggacgagc ctccagaaca ggcagcaacg agatcagcgc aagacggtgg
     1801 ccagcagagt aacaaccacc tgccagcggc atccgcgtct taccctgcag gtggcgcggc
     1861 atcggcttta ctcgtcccaa ttgatacccc gggcagcagg ggagagagac gcctgttgct
     1921 gcacctgagc acgcaccgca gcgaacgccg gagcacgcga cgcatcaggg agtccgcgag
     1981 aggaggccag cacgccgtca agcatcacca gatcggcagg agccacgccc gcctgtttca
     2041 tctcagtcag gaacgccgca tcctggatgg actgagcacg cgcccggtga accgactccg
     2101 taggagaggc aaccggagcc ccgatcggcg cgaacgtctg cgcagcaccg cccgtctcca
     2161 gcacctgcgc cagcgcgtcg ccggccccgg tagattttgc cagcgtcgcc tcatggcgaa
     2221 gccggtcaca cagcatcgaa aatgcagaca tttacgcctc catgccgcga ttgcggcgca
     2281 gaatttcgat cgcaccctgc gtggagaaca gctggttcag gcggcgatca ggattgataa
     2341 cagtacggta caaactgcgc cccgcgtcgg actcggcaag cagcgtcgcc tcatccatcg
     2401 ccagcttcag cagctgctca atacgctgta cgcgcacggc gtgcgccagg ttgaacaggc
     2461 cgatcgcagc cgccgcatca cggcggtcag catcctgcag cagcgcgtca cgctcccggg
     2521 ccacatcagc ctcagcagcc tcggcggcaa ccagctcggc ctcgatatcc ggcagcagga
     2581 acgcagcgac gcccgcatcg gggctcacag tctcgccagc acgcagcgcc gtcttgccgt
     2641 cggcaatcag cccgcgcagg cgttcagctt ccgtcctgag cccttccacc acgcgagcgg
     2701 cgtcaatgcg tcgcgtttcc acgtcggctg ctgcctgacg catttcacgc gccacccggc
     2761 gatcgtcaag cgcggtaagt tgagcggagg agttaggatc caccgacagg caaggaaggt
     2821 cataaacggg aaaatggtca ctcgccattt cagcaagcgt ggtacgcagc caggtcgcgt
     2881 ccgggcattc ggttttgatt tcggtcatgg tttggttctc agcaagttat cagcaaagcg
     2941 gcagcaaacg ccgctcagac agcatgccgt cacgacaccg cgcagacatc aacgcagagg
     3001 gcgcagggct ctcgcccagc gcggagagcc gcctggaggc cgtcgctaat cccctacatt
     3061 ccgggacacc cgacaaagcg acgaaataga cactttagcg atagactttc ggtacagttg
     3121 tggcatgttg tgtcgaaaga gacgtatcgg tcagaggaga gaaaaaagtg agcaaaacat
     3181 acggttacgt tcgcgtatca accgacgaac aaaccagcaa cagccagcgc gacgccatca
     3241 tcgcccggtt cccaacagcc tccatcctcg ccgacgacgg gatcagcggg acggtaccac
     3301 cacgcgagcg tccagccctc ggcatcctcc tgaaccagat cctgcaggag ggcgacacgc
     3361 tggtcatcta cgcgatcgac cgcatcggca ggaacgcccg ggacgccctc gaactggtcg
     3421 aggagctgga gaaacgaaaa gtaaccctgg tcagcatccg cgagggtgtc gatttgagca
     3481 gcgccgtcgg acaggccgtc ctcggcatca tggcgagttt cgcgcagatg gagcgcaagc
     3541 tcatactgga acgccaggcg gcagggatcg aggccgcaaa gagccgtggc cagaagttcg
     3601 gacgcccgac caagatcggc gcgaaagaag cgggggagat agtcatgcgc cgcgccatgg
     3661 gggtaccaat agccaccata gccaagcagt taaatctgag caggagcgcg atttaccgcc
     3721 accttgagaa ggtcgacgcg gtagcgccgg agtaagagag agcgcagcgg tgtaggtggc
     3781 tccaaccatt ccacaccgcc gcaacccagc ccggggaacg catgaggagc agacccgggc
     3841 accattccca acaaggaaca gaaccatgat aaaccaagca gcaacaccgc gctatcccgg
     3901 cctgagcatt gaacacggcg agatccgcca gttcggcaag ccccgccatt gcgccgccaa
     3961 ccgcaaaggc cagtaccaga ccaccctccg cgacagcgcc ggtcggtacc agacagtcag
     4021 catcatcagc ctttacgcct gggaagcatg accataacga aagccttaca ggtgtaagca
     4081 gcaagcaata acaaaagcct tacgccatta cgaaccactt acaggggcgc gggagcgccc
     4141 ccagaaacca gaatcataag gagagaacga tggcatttga aaacattccc cgtaggttcc
     4201 agagatggaa ggtaatcatg gttcccggag caaagtcata tgagtcatgg gacgagatgg
     4261 ttaccggcga gaacttctcc gaagaaacca ggatgaaaac gtacaaaaag caccgcacca
     4321 aggccatcga ccgcaccgtg gcaaaccagg agtacatcca taccatgacg gaggacggga
     4381 tcaccgtcag gtaccgcctc gaacgctggc gctaacccca gctaggagcg cagcgactgc
     4441 tgcgcctcct cccttaccca gaacgcacca gagccattct aagcgcctca gccacaaaaa
     4501 tacccaaccg cacgaccacg cacgcacaaa cgccgtgtac cccccaaaaa cccccgccat
     4561 tttcagcgcg tgacgtttca atacaggaga gaaaccaacc ggagcaggac accatgaaga
     4621 aatacaccac cgagcagaaa gcccaggcgc tcagactact ggagcaggac ggcgcaaccg
     4681 cagccaccgt cgcacgcacc atggggatcc caccccgcac cgtcagagat tgggcgaaag
     4741 cccgcaccga cgcacccagc aacgtcctca gcatcgagga gatgcgcgaa cgcgcacagc
     4801 gagccgtcga ggccacccca caggcagcga tccgacgcct aaaaaaccac tttgtacagc
     4861 gccagtttga gctgctacag cgccacgcca ccgacctgca agccctccgc acagcttcgc
     4921 tacaggccat gctggagaag gacgccacca tggtcaaagc gatcagcggc cttatgacat
     4981 ccctactcaa gacgcaggaa cgcgagcgcg tcatctacga gatcaagcca ggcaccgagg
     5041 ccgacatcat gcgcgagggc atgaatcgcc aacagagctg aaaacggcgg gaaaaccgcc
     5101 ttttaccgcc gctttaccgc cgcagaaaac ccgggagccc gcgaaatacg cggatccgca
     5161 cgttaacgag ttgttaccgc tcgcgtgcgc gagggcaagc cctcacgcag aaccaacaga
     5221 cgaccaccca gcgcgaggcg aaagcctcgt gccctcagcg ccacccaaac ctccgatccc
     5281 cctcagaacc actaccaccg accatcccgg cacgacgaac ccatccaccg atctaataag
     5341 acaagcaaag tgcatccggc agggtgcagc tgcggcgcgt cgattccaag atgatccacc
     5401 cgttccaccc gatccggcat agattcataa atagtaaaaa aaacaactaa ttgttcaatt
     5461 agtgagcatt taccccctat ttacccaatt tattcatttt ttgtttttaa ttagaaaata
     5521 gggtggaacg ggtggaacac aacaatgaaa gcctttaaac acgaggcctt agctgttcca
     5581 tccacgggcg gaacacgggt ggaacgggtg gaacacccgg gtggaacagg tggtacaaaa
     5641 acggcgtgat gatccacccg tgatccaccc gggtggaaca ataaaaaagg ggagcatata
     5701 gctcccctct tacggaaaag taacccttac acgacagctt gcgttcgacg gaagtagcca
     5761 cgaactaacg caccgtttat gcgcttcctg ccaacctcat agcccaatcg ggacatgata
     5821 gccgccatgc gctgatgctg cttgttgctg gtatggccaa tcatgagccc tacctgagag
     5881 aacaaggcct tcgtcggcac gaaagcagtc gggtcgcagt cgtcaacgtc cagcttgagc
     5941 agctcggcga tctcgtcttc ccacggatcc tcgacgcgga actcgtcatg caccttacgc
     6001 gccagcgttt cagcggcctc ccacatcacc ccattctcat tgaacaggtg gagcgcttca
     6061 gcccacagct gcgagcggtt tttctcgatg gccgcgcagt ccacgtcccc ggaattcatg
     6121 ggcaaatagc ggcggttccc cgtcgagtca ttgaggatct gcttgttgtt cgtggtaccg
     6181 atgaacacca cgcggcgcgg gtttcgcacc ttgaactctt taaatttcgg cacccactcg
     6241 tcgtgggcag cggagaagaa cgctttcacc gattcggagt cgcgcccgtg gaggccgcgc
     6301 agctcctcca gctcgccaat caggcaccca cgcatacggc gcactaggga gtcgtccttg
     6361 tcgttcaggt tgaagctgga gtaggagtca gggaatggcg acataaggcg aacgcccgtc
     6421 gatttgcggg caccctgtcg cccgtacagc agcggaacca tgtcggcctt gattccggga
     6481 ttcattacgc gcccggcaag cgccgtccag gtgtagaggc tgaccgccgc caggtactcg
     6541 ggatcccctt ggcatttgaa gcaccgctcg aagaagtaac tgacgcgagg tttgccgtcc
     6601 cattcctgag ccgtcagcca acgctgggcg ctgtcgaatt tgttctcatc gcagacgtag
     6661 aacatggcgt cgcgggcatt gtccttggtc accttctcga agtcggcagc agcgaggcgc
     6721 agacgcagct cggtaactag gttgtcggac agcgggtacc attgcccgtt cacgaacgcc
     6781 ccctcctccg caaagaactc atcgaaagcg agctggatgc cggtgacgga atggcagcgc
     6841 atagcggcga caacgttctc aagagaggac gagtagcgcc ccttccggta gttgatctcc
     6901 agctccacgg gaaacgcggt agtcgggtat tttttttgta gagacgggcg tcccaccttg
     6961 gcagcggcga tcggcgttac gttgtcggca gcagtgccgg aattttcatc aatttgtgac
     7021 acgcaattac tcctttaaaa acgtaggagt cggcgtcagg cgggggctta cgagggaaat
     7081 tcacacttgc gcgatcttat cgacgtggta cacttctctt tgagtttgag gagtacgctg
     7141 cgcaagtgct attcttcctt cgagaacgtc ccgctaagac gtgactcgaa ttaatcggag
     7201 gagttaccag ctcccccggc agaaaaaggt gaccacccaa caggtcgcct ttttttgtgc
     7261 ccgaagatcc ccaaccggat ccgcagacgc cagatcagct tacccgatcc caaccctcca
     7321 cgccacttag cgccgcgcac ttatcgcgct gtcgtgatca cgcccagctc aatctctgcg
     7381 ccgcctctgc tatttatcca tgtacacaaa cggcaccccg gcgacgcaaa gccgtcgccc
     7441 ctctgtgcac gacaactgca cgacgtcgaa ttcgacgcgc acgacaaaac gcattacact
     7501 acgcaagaca aacagcacga caaaacgcac gacgtggaga gcagcagcca tgagagagaa
     7561 aaccaaaacc ctgaccgttc gcctacccgt tagcctgatc gaacgcatcg agcagaacgc
     7621 agcacgaacc ggagcaaccc gcacggatgc tgtcatagag gcgctgaacg ccggacttgg
     7681 cacgaagccg gaagggatgg acaccaaact tttagctgcc atcgaagcgc tgagggctga
     7741 agtcagggcg ctgtccgtcg gcggtaaacc tgcacgacaa accgcacaac aaacagcacc
     7801 acaaagcgca caacaacctg caagacagat agtcccccgg ggagccgatc cgcgcttcca
     7861 ggccgtcagt gatgaggctg accagctcat tgtcagcatg accgcagaga acgcgactct
     7921 gcccgccatc gcgtcggcac tgaacgcagc agggcaccgg acagcgacgg gacaggagtg
     7981 gaacaccagc cgtgtccggg actatcgcca taagttgcgg gaaagtgggc tactgaatta
     8041 gcagtcctaa gtgacatagc taaatatgat aaaattcgaa cataaaaatt ttacaaggaa
     8101 aagaagtggc tatcggtgat gatgctatcc gtgacgcttt ctatgctttt acacagcaag
     8161 ctgaagaaat ggatgataaa ggtctccctc aggaagtttg ggagacgcct tgttttacct
     8221 atctgatgac taagaagcag ttcaatcaaa tgaaagttgt ttgtcagaga aacggctggg
     8281 acatacctgc atcaccagca ataccgatca cttgggcaat gtttaggcat gtgctatctg
     8341 ctcgtaactc gaaggataag ctaagttggc aagagtgtgc ggagattttg gcaactgcat
     8401 tctcagtgca gagcaatgtg tacgttaaca gagattactc ggagcagact attgtgctca
     8461 atgctgttcg tcgcattaac gttgctgggg ctggcttttt cgcaatggcg atagttgatg
     8521 tctctgaaaa taatttggcc cccgtgactg cataccatgc aacggaggcc aaatgtaaag
     8581 ctatacagcg agggtgatag gaaggacgcc catttaagca attctcctca atttaatgag
     8641 aatgaaccat aacgcctaaa tatttccctt cattggttcc tttcggatct agcagcatcg
     8701 gcggttttct gctgtctatc aaattcactg ttattactag gaagccgcaa cataacttcc
     8761 caacaggaga ccagcctcct gactgcccag cgcaaagggt taattactgg ttaatacccc
     8821 ctgcgaatgc tcttgcagta caacaatagt aggctattgt tgacgtagat tcaactggtt
     8881 tgtttataac caagcggcac gaggagcggg tttacaggtt tttatagctc tttagaggcg
     8941 gttaaccata ttgcgatatc aggtaattat agatcttgaa atcattaaat ttatcaatta
     9001 tgattaattt tatgattaat ttttgatcta tagaggtact ttgatggata ttacgatatc
     9061 tgacgctaca tatagccgtc ttgctgcaca agcaaaggga tttgaaactc ccgaacaggt
     9121 aataaaccgt ctgatcgatg ttgttgaaat cgatcatgac acacgccctg aacttttttt
     9181 taatcctgaa ggtgaagcgg attttaaaaa agcgttacta aaatcaaaac aggccaaagt
     9241 cgaaatgttt aaaagggatg gttcctgtga aactggagta tggaatgctc gtgcgatttc
     9301 agaagaatca tcagttcggg caaatatttg gagtgggtat ttgcgtggat ggaaggaaaa
     9361 agggattgtt cgtgcggttt tcactgtcca ggaggttcct gacttgccag gagcggctga
     9421 aagtgtgcag tggaatgatt atacaatcag tcgcactttt agtggcggta tttcagttca
     9481 aagaaatggc atgcctgtaa ctattgtgaa gccatttcta cgtgatgttg cgcatgattt
     9541 aaaaatatca ttgctaaata gcaatggaaa tgaaatgaat actcggcagc ttggcgcagc
     9601 gatcataaag aagttaaata actcttaaca tcgatgcttc acgcagagga gcgacggctc
     9661 aacgccgccg ccggtcagag ttctactgcg acgaatacgc accattcata gctggcgagg
     9721 cagaacgccc gccacctgtc atcagacgcc cggtcaacac actccagcca taaccgcgcc
     9781 gcagcaaggt ggtcgcagcg caccgggtcg ccccgcagtt ccgtcgccat ggcctcctcg
     9841 atcagtcgcc cttcccactc cagctgatcg aggatcgagc ccctacgccg ccgccccatc
     9901 gagccactgc agcatcgccg ccctgatctg ccccgcctcg ctctcgtcca gcgtcaggaa
     9961 cgccagatcg gcaaccttac ccagcccggc gaacgccacg cagccggtga cggagaacag
    10021 ctccacgagg agcgccgtcg ccagcgtctc ccgttcaacg ccgcacaacc aggcgtagtt
    10081 acgctcacgc gatgccggaa gtttcacctg cagcgcacgg gtggccagag tgttaaattt
    10141 ctcggtacca gcccactcgt agtcgaacgg cgggcgatca accaccaccg acccggacag
    10201 cgtctgctgg cgttccccag cggagaagtt cggcagcttg aggtgggaga acccatcgag
    10261 cgaccactcc gacatttcag tggcgatcac cgccagagag cgagccgtcg cggcctcgcg
    10321 tagttcagtc tttgatttca tgtgccctcc tttgggctaa aaaaaaaccc gacatcaaga
    10381 cgaggccggg ctttatgctg ctgtgagaaa ccgatcgcag gatagcgtca cgacggcagc
    10441 gccggtgcgt cgctttagtc cgtctcatca aaaaagcatc ggtagtgtct cgaaagtcgt
    10501 tcgctaaacc agacgccacg acggcaaaaa agatgtcccg aaaggcgcac cattcggaca
    10561 cgccaatcaa cgccgctcaa gccgggcatg acgaaccgcg tagcgcagca gctcacgagc
    10621 ccgatcttcc ccttcctcaa ccagcacgtc gaaccagtcg aggttatccc ggggcgtgaa
    10681 gcgatcgagg ccgtcgaacg acacaaccgc cgcgcctgga attgtcggga ccggctctcg
    10741 ctcatcgcca taggccgctt tatgcgccag gcggtacgct tcgccgtgat ccggtaccag
    10801 catcacatca acatctggcc agaaccggcg cagcgacgcc acgcagctgc cgaacccggc
    10861 aattgagaac gccgcgcaga ctgccacctt gcccttcgtc agatgatgca gggcaaccgc
    10921 cgtggcgaac ccctcacaga cgaacagagg cgagtagggc gcatgtggtg aggtatcgag
    10981 cacgtccata aagccgggca tcggatagaa ccgtcccgcc ttttgggtac ctgcagggga
    11041 gaacttgtcg ccggttttgc cgccagcaga gcagacgccg cagatggcct ccagcgcgtc
    11101 gccaaccagc tggcgatcat cggtcaggcg ataggcagga acaagcagac agcgcgacca
    11161 gtacgccctc ggttcgaacg agcggaacaa gccacccaca cgaaggtcag cggggagggg
    11221 cgggaggctg acgccaccac gcctggcgat tgtggtctta ttgcgccagt acggggtata
    11281 ggccggatta ccgtcgcctt tcgccagcat cctgaccgca tcagccgcag ccttatcggc
    11341 cttgcgccgg tcggcatctt cgcgtctggc caccgcctcg atcgatcggc gtttccgttc
    11401 agcaagagca gcaacctgtt cagtcgacgg cggcgtgcga tttgagtcga gccgccagta
    11461 cacctcgcct gagaaccgca ggaatacttg cgccatggcc tcgggattgg ttacgccttt
    11521 cagcttcagg aagtgccggt actgcgaccg gtgatgcgca gatccgcgat tgtggcgggt
    11581 agaccaggcc tcgatagcag caagcccgtc ctctccgcag agagatgccc agcatgcgat
    11641 gccagaacag aggcgcagcc agtcctcgcg gtcaaccgga gccccagccg cagagacggc
    11701 acgccagaac aggccagcgt cagtcaggtg agagttatcg aacgccataa ggagccctcc
    11761 cgggacgacg ctggtcggca ataaagcgtt tgagcacggg atccccagcc agccagtaat
    11821 gcccgccacg aagggaacgc cctgccggag tcggaacgat cccccgcgaa ctcaggcggg
    11881 agagcgtgcg cagcgacacg ttcagcagcg cagcgaggtc agcggtatag attcgttttt
    11941 tcattgggag taccagatag gtgcccccct cagcttaacg ggcatcgggg ggcgggagtg
    12001 acgattatcg ataaagccgc ccttgaggac ttaccgcata gcggcaggac agcagccctt
    12061 ctcgcttact cgcacctgcc aaggacgaag cgcgcatcct cgtttacaac cccgcctcgt
    12121 tggggaggag ggaaagcggc accgggcgat ccggtttcat gtttcccaac tgctcgtcag
    12181 gaatcagtaa gacaagctta acacaattaa cgccaaagtg gcgttttttg tgagatacta
    12241 aaaaagacgc acagccagtg ctggcgaggt ctggcgacca tttcgacgca gggggagcag
    12301 tgaacgacga taacgacacg acagaagcga cgcagccgtc aaccgaggca ctgaagtgcg
    12361 taggcgctgc ggagcggttc cccgacatca tgcccggcgt tttgttcgtc acctgccccg
    12421 gcgggtgctc cctccgggtc gaggcatgcc gacagattta tgagcagagc cacctcgatc
    12481 gcacgattga aaacccccac ctcgagtgcc accggtgccc cactggcctc gcgctttggg
    12541 tcggtaccga acagccccct gacgcaccac tgaccacccc ggcagacacc cgtaagctct
    12601 gcccccgttg cagccggaaa gacaaccgcc tgatcgcccc caataagccc ggttcgagcc
    12661 tgtgcgtctc ctgctggaac cgcgaacgcg aagccaccat cggagccaac gcacgcggga
    12721 gagtcaaccg caccccaccg ttcgtctcgc cgttcctgat cggcatcggc gatggctggc
    12781 aggtctggta cggcatccac cagggcgagg cgctcgtccg cgccatgcga gccaacccgg
    12841 gcgcacggtt ccacgatcgc cgaccaggcc cggtcgggc
//
