LOCUS       NZ_OX030736             2101 bp    DNA     circular CON 16-JUL-2023
DEFINITION  Escherichia coli isolate 68 plasmid P8, complete sequence.
ACCESSION   NZ_OX030736
VERSION     NZ_OX030736.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065790
            Assembly: GCF_905330955.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/16/2023 03:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,399
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 70 of 177
            Pseudo Genes (incomplete)         :: 116 of 177
            Pseudo Genes (internal stop)      :: 35 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 4 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX030736.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="68"
                     /isolation_source="Hospital de Guadalajara (Guadalajara)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P8"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            339..1304
                     /locus_tag="LQ200_RS27525"
                     /old_locus_tag="AI2959V1_5304"
     CDS             339..1304
                     /locus_tag="LQ200_RS27525"
                     /old_locus_tag="AI2959V1_5304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1308..1574
                     /locus_tag="LQ200_RS27530"
                     /old_locus_tag="AI2959V1_5305"
     CDS             1308..1574
                     /locus_tag="LQ200_RS27530"
                     /old_locus_tag="AI2959V1_5305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          733..964
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 ttcgggggtg cttgttttgg ggggttaaaa ttgtggttat tttttgcgca attctcgcgc
       61 gtgatccttg tatttatact taagggataa atggcggata tgaaatagtg gtttagccca
      121 gtaatgacga ggctttgagt gggttttgac aggtcaaaga aaatggagca gaattgaggc
      181 gtttttaatc ggcgttgggg agtgcgtcaa cactccccaa catttcgaat gtgtcacctc
      241 agcggcaaac tctggtgaca tgtactggct cgcaatgcac aggtacgtga tgaatatacc
      301 acatcaaatc acagcctgcc cagatcggag caggcttaat gtcagaagat aaattccttt
      361 cggactacag cccccgtgat gcagtttggg atacccagcg cacgcttacc gattctgtcg
      421 ggggtatcta ccagactgct gctgaattcg agcgctatgc actccgtatg gcctcctgta
      481 gcggtttgtt acgttttggt tggtctacca tcatggaaac cggagaaacg cgcctacggc
      541 ttcgtagtgc gcaattttgc cgtgtccgtc attgccctgt ctgccagtgg agaagaaccc
      601 tcatgtggca agcccgtttt tatcaggctc taccgaaaat cgttgtggat tacccgtctt
      661 cccgatggtt gtttctgacg ttaactgtca ggaactgcga gataggtgaa cttggaacag
      721 tccttacagc aatgaatgcg gcgtttaagc gaatggaaaa gcgaaaggag ctatcacctg
      781 ttcaggggtg gatcagggct acggaggtga cgcgaggtaa ggatggcagc gcacatccgc
      841 attttcactg tctgctgatg gtgcaacctt cttggtttaa agggaagaac tacgttaagc
      901 acgaacgttg ggtagaactc tggcgcgatt gcttgcgggt gaactatgag ccgaatatcg
      961 atattcgggc agtaaaaact aagacaggtg aggttgtggc caacgttgcc gagcaactgc
     1021 aaagcgcggt tgctgaaacg ctgaaatact ccgttaaacc ggaagatatg gcaaacgatc
     1081 ctgagtggtt tcttgagctg acgcggcagc ttcacaagcg ccgttttatc tcgaccggtg
     1141 gggcgctaaa aaacgtcctc cagttggatc gagaaaccaa tgaggatctt gtcattgccg
     1201 acgatgtagg ggatggcact gatgacggga agcggacggc gtttgtctgg gattcaggta
     1261 aacggcgtta caaacgcgcc cctgagaagg ataaatcgga ttaacgtatg aatattaata
     1321 ttgaatacct gaatggaaat aagactattg gtttattttt tttaagaagt gaagcggtga
     1381 ttcctgacag gtttaaaaac cttattttgc ttattgatgg attaagtttt ggcacatttg
     1441 gttttcatcc gcacgaaggt tttgaggatg aattaatttt atatattcag aaaacaaacg
     1501 agagggtaaa aactcttttt gtgaaaatag atttaaacgg tatcaagttt ggatttttaa
     1561 gaacgcattc ttagttctgg aaaagagcca gcggcaggct gaggtgatag gtacgagatt
     1621 gcatgcaatc tctagtgctc tgtctatcct gcattatcct cagcattatc ctcagcatta
     1681 tcctcagcct tgccaactcg acaccaatgc aggatagaca atccgatgtc aaatgttaac
     1741 actctgcgag tggtacattt tccccggatt atcgtcctga gcctgccgct ggctctcttt
     1801 ctaccgcctc gctttgctcg ttgctcaacg cctcacagac acggattaaa atccgcatcc
     1861 gttcaccgtt ttttaaagtc cgttaaaagc atgatgccat ctccgagagt taatctcgtc
     1921 aaatgctaaa tcgtgggggt cccctttggg gttccgattt agtgattgac gacaccaccg
     1981 attaaaaaac ttatgcgggg tggatggttt cacgaagtga ggccatccac ctgtaagaca
     2041 gggttttgtt tttattccct gttttagtga tcgggtgtgt ggaaaaggtt ggggtaagcc
     2101 g
//
