LOCUS       NZ_OX030735             2317 bp    DNA     circular CON 16-JUL-2023
DEFINITION  Escherichia coli isolate 68 plasmid P7, complete sequence.
ACCESSION   NZ_OX030735
VERSION     NZ_OX030735.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065790
            Assembly: GCF_905330955.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/16/2023 03:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,399
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 70 of 177
            Pseudo Genes (incomplete)         :: 116 of 177
            Pseudo Genes (internal stop)      :: 35 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 4 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX030735.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="68"
                     /isolation_source="Hospital de Guadalajara (Guadalajara)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(2243..2317,1..462))
                     /locus_tag="LQ200_RS27510"
     CDS             complement(join(2243..2317,1..462))
                     /locus_tag="LQ200_RS27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(751..987)
                     /locus_tag="LQ200_RS27515"
                     /old_locus_tag="AI2959V1_5303"
     CDS             complement(751..987)
                     /locus_tag="LQ200_RS27515"
                     /old_locus_tag="AI2959V1_5303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1749..1862
                     /locus_tag="LQ200_RS27520"
     CDS             1749..1862
                     /locus_tag="LQ200_RS27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(1218..1396)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.066"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          1373..1862
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 tcccattaca cccagaagcc caccctccca aacactataa gctctctctc catttaacat
       61 atacacgttt tccggaattg ttcttaatgt atatacgggg ttttgaatac catattcctg
      121 cccaagagtt ttaaaatact ctccttttaa gtaaaactta tctttccctc tgtaactaag
      181 ataaacctta ctaaaatcat aatctttcac tttttcagca aattgcagca tcaccctact
      241 aacatccagt gaactatttt ctgcagaaac agaacgaagg tcataattaa tctcggaaga
      301 gtttacaaac cacttataat aaacccaaac gctaatacca tcattccttg gatcttcttt
      361 cagaacctca ttcatgtgcc tttgaagaaa aatgtagttg gcagataaaa caccaccaca
      421 taagagtact attaatatga ttgctgtata tacttttttc atttctataa tccatctcaa
      481 ctgctctaaa gtttaccctg agatttttaa gctaaggtgc cataagttag caaggaatta
      541 agcatcgatt taccataacg tcttcttacg cgcaccgcga aaacggcctc agggcgtttc
      601 tgcgccttca gccacaaaag agcacgtctt acccccagaa aaccgcccgg aatcgctctg
      661 gtgagtttta cggtggcgaa tgactatgca ccggagtgaa taccacgctg tttcgccctg
      721 aactccccac aaaaccgacg aaaatggcaa tcagcgacgc ggcgcccgcg gacgcgacca
      781 ggtaaattcc gcctgctgcc gctcctcctc acgcgcagat cgctgccgtt ctgcctggcg
      841 ctgcgattcg atctgtgcac gagccctgtt aaggacaatg tgcctgttgg gctgattcag
      901 cttcttgcct gacgctctct cagcggcggc aacagcatcg tcgagaaatc ttttctcggc
      961 aggcgtcagg tttatcagtc gtttcatggc gttctccggt cagaataact ctgcacagtc
     1021 tgccagacac ggcgaacctc atgaagaacc tgcagccggg aacggccctg agaaacggtg
     1081 aacgggaacg gattttaatc cggtgccgtg aagcgttggc ggggtatcgg ggcgcagccc
     1141 tgagatatcg ttttggtgcc ggcatcatga tggcggtact ccaaaaacac atcttgtccc
     1201 gctttcagtt tctgaatttg cgggttccgg gcgcagcgcc ggaccagtca cgtagcgcta
     1261 gcggagtgta tactggctta atatgttagt tgggcgtggt ggctcaggga agtgcttcat
     1321 gtggcagggg aaaaatgcgg ctgcggtgcg taagtgcaaa atgctgatga tggtgaaatg
     1381 acgcctcctc gctcactcgg tcgctacgct cctgccgtga gactgcggcg ggcgttacct
     1441 gctcacgaat gacgaggtaa cgaaatgaag ctgaggtcaa gaagggcccg aaaaaggatg
     1501 cggcgctgcc gtttttccat aggctccgcc cccctgacaa gcatcacaaa atctgacgct
     1561 caaatcagtg gcggcgaaac ccgacaggac ttaaagatcc caggcgtttc cccctggtgg
     1621 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     1681 gccgcgttta tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     1741 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgccttatcc ggtaactatc
     1801 gtcttgagtc caacccggta agacatgcaa aagcgccact ggcagcagcc actggtaact
     1861 gatttagagg agatagtctt gaagtcatgc gccagttaag gctaaactaa acgaacagtt
     1921 ttggtgactg cgctcctcta actagccagt tacctcggtt caaagagttg ctgctctgag
     1981 aaccttcgag aaaaccgcct tgctgggcgg ttttttcgtt tacagagcaa gagattacga
     2041 cgcaacagaa aggatctcaa gaagttcctt gctgtattgc tactatccta gagatctgac
     2101 ccattagggg cattatcaga tgacggcctt gtaatccacg gggagcaggt cacacatcta
     2161 aagcatctac gagtaaaaac accccagtga ccaatgagcg atttgcgtga gctaatcgtc
     2221 aatgtatact gatcgcttca aattaatcgc tcatgctttt aatgaaatca tctaagtacc
     2281 aagccttaga gaaatcacta agatcttcca tctgttt
//
