LOCUS       NZ_OX030734             3223 bp    DNA     circular CON 16-JUL-2023
DEFINITION  Escherichia coli isolate 68 plasmid P6, complete sequence.
ACCESSION   NZ_OX030734
VERSION     NZ_OX030734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065790
            Assembly: GCF_905330955.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/16/2023 03:42:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,517
            CDSs (total)                      :: 5,399
            Genes (coding)                    :: 5,222
            CDSs (with protein)               :: 5,222
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 70 of 177
            Pseudo Genes (incomplete)         :: 116 of 177
            Pseudo Genes (internal stop)      :: 35 of 177
            Pseudo Genes (multiple problems)  :: 40 of 177
            Pseudo Genes (short protein)      :: 4 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX030734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="68"
                     /isolation_source="Hospital de Guadalajara (Guadalajara)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P6"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            316..801
                     /locus_tag="LQ200_RS27485"
                     /old_locus_tag="AI2959V1_5298"
     CDS             316..801
                     /locus_tag="LQ200_RS27485"
                     /old_locus_tag="AI2959V1_5298"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            805..1020
                     /locus_tag="LQ200_RS27490"
                     /old_locus_tag="AI2959V1_5299"
     CDS             805..1020
                     /locus_tag="LQ200_RS27490"
                     /old_locus_tag="AI2959V1_5299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1188..1520
                     /locus_tag="LQ200_RS27495"
                     /old_locus_tag="AI2959V1_5300"
     CDS             1188..1520
                     /locus_tag="LQ200_RS27495"
                     /old_locus_tag="AI2959V1_5300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1729..1899)
                     /locus_tag="LQ200_RS27500"
                     /old_locus_tag="AI2959V1_5301"
     CDS             complement(1729..1899)
                     /locus_tag="LQ200_RS27500"
                     /old_locus_tag="AI2959V1_5301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2538..2861
                     /locus_tag="LQ200_RS27505"
                     /old_locus_tag="AI2959V1_5302"
     CDS             2538..2861
                     /locus_tag="LQ200_RS27505"
                     /old_locus_tag="AI2959V1_5302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             1189..1520
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          2251..2442
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1788..2268)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..1118
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.374"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2852..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="99.0"
                     /evalue="5.02e-72"
ORIGIN
        1 accgcgccga ccgtccgcgc atcgatgcct ggcagaccat agtgaacggc aggctggggc
       61 tgcatgaccc gaacgcgccg gagaaccggc gggtgctggt ctcgccttcc gcgctgccgg
      121 aagcgaagca ggaagccgcc caggcgatta cgagcggcct gcttgccctt gcctcatccg
      181 gggagctgaa aacgcgtcag gacgtcactg aggcgctgga aagcgcaggt tttgaggtgg
      241 tgcgcaccac aaaaagcagc atcagcattg ccgacccgga cggggggcga aacatccgac
      301 tgaagggagc catctatgaa cagtctttta acgctggcga aggacttaga gcagaaatcg
      361 aaagcgcagc agcagactac cggcgagatg ctgaaagccg cattcagcga gcacgagaag
      421 tctgtcagag cggaactgag cgaaagcgag aagagaatca gcgccgccat cctcgaccac
      481 gaccggaagc tgtcctcagc catgagccag cgcacgaaag ggatgctgcg catggtcagc
      541 cagacgtggc tgaccatcgt tctggtctcc gcgctgctga tagcgtcgag cgcgggcatt
      601 ctgtggtggc aggggcagca gatactcgag aactatacga ccatccggga gcagaagagc
      661 acgcaggcca tgctgtcaga gaggaacagc ggcgtacagc tctcgacctg cggcgagcag
      721 agacgccgct gcgtgagggt gaacccggaa gcgggacagt tcggagagga ctcgagctgg
      781 atgatactgg cggggaaata gcacatgacg gagctggaaa aacagttgct gagcgcatta
      841 gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagaacgc cttcgcggaa
      901 tggcggacga tgtctggtct tattcaacgg gagaacgcgg cgctgaacga gcgcgtcacg
      961 gtcttgagca ggcaggtgca gagtttgagc gagcagctgc gccggttgtc gaaaggctga
     1021 acgcgataga aactcaccgg cagcaggagc gggcggtgca gcaccagaaa gcgctggagc
     1081 tggaacgatc gcagtggcag catcatggtc cttcgctatg ataagccggg ttctattaat
     1141 tagttgttat gtaaagatat ttaaattttt caacatgagg tatttttatg aatacatata
     1201 ttttcctggg aggggctatt gtggctgaag tcataggcac cacctttatg aaatattcag
     1261 aaggatttac acgcctgtgg ccgtctgtag gaactgcaat aagttattgc gctgcctttt
     1321 atctgctttc ccaaacattg gaaaatatac ctaccggtat tgcttacgca atttggtctg
     1381 gtgcgggaat tgtactaata agcctagctg gttggttggt tagcggtcaa aaacttgatt
     1441 ggcccgcaat tgcaggaatg ttccttattt gcgttggagt tttggttatt aatatttttt
     1501 caaaatcttc tgcgcattaa ctttttaccg aagcatattg gccaaagcaa aaggatttcc
     1561 ttgagatcct ttctttctgc gcgtaatctt tttccctgta aacaaaaaaa ccaccgctac
     1621 caacggtggt tcgtttgccg gaattcagag ctaccaactc tttgttccga tggtaactgg
     1681 ctttcacgga gcgcagatac ctaaactgtc ctttcagtgt agccgcactc aggctttcac
     1741 ttcaagaact ctgtaggcat tctctacaat ctctgctccg tgaacccagt taccaatggc
     1801 taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     1861 acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccatcc tggagcgaac
     1921 tgcctacccg gaaccgagtt tcaggcgtgg aatgagaaac cgcggccata acagcggaat
     1981 gacaccggta aaccgaaagg caggaatgcg ggggagcacg agggagccac cagggggaaa
     2041 cgcctggtat ctttaagccg catcgggttt cgccaccgct gatttgagcg tcattttcgt
     2101 gatactcgtc aggggggcgg agcctatgga aaaacggcaa ctccgcatcc ttttcttggc
     2161 ctttcctgct tcgagcggca tttgcttatc ccgtaatctg tgagctggta acgcccgccg
     2221 cagtctcacg gcaggagcgt agcgaccgag tgagcgagga ggcgtcattt catcaccacc
     2281 tgcattttgc acttacgccc ggcagccgca tttttcccct gtcacatgaa gcactttcat
     2341 aaattgccac tccgtcataa catgtttagc cagtatacac tccgctagcg ctacgtgact
     2401 ggttcagggc tgcgccccga aacccgctaa aaccactgcc gcgcctgcgg cttgtccaac
     2461 accgtgccga ccgggaaaag atttcccgtc cgtcccggcg ttatcaggag gccggattgg
     2521 cagacaaacg cagcaaaatg ctcaccatgt gggtgactga ggacgagcac cggcgtctgc
     2581 tggagcgctg cgagggtaag caactcgcgg cctggatgcg gcagacgtgc ctggacgaga
     2641 agcccgcacg cgccggcaaa cttccgtcga tctcgccggc gctgcttcgt cagcttgccg
     2701 gcatggggaa caaccttaac cagattgccc gccaggttaa cgccggtggt ggaagcggac
     2761 acgaccgcgt gcagattgtc gccgcgctga tggccatcga tgccggactc gagcggttgc
     2821 ggcatgccgt actggaaaag ggtgctgatg atgatcgtta agtttcatcc tcgggggcgc
     2881 ggcggcggcg gcgggccggt cgattatctg ctgggtaaag atcgccagcg cgacggcgcc
     2941 agcgttctgc aggggaagcc ggatgaagtc cgggagctta tcgacgcctc gccctacgcc
     3001 aaaaagtaca cttccggcgt tctgtctttt gccgaacagg atttaccgcc cggccagcgc
     3061 gaaaagctga tggcgagttt cgagcgggtt ctgatgcccg gactcgataa agaccagtac
     3121 agcgtgctgt gggttgagca ccgggacaag gggcggctgg agctgaactt cctgatcccg
     3181 aacacggagc ttctgaccgg caagcggcta cagccgtatt acg
//
