LOCUS       NZ_OX030706             4087 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Escherichia coli isolate 131 plasmid P5, complete sequence.
ACCESSION   NZ_OX030706
VERSION     NZ_OX030706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065784
            Assembly: GCF_905330895.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:37:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,311
            CDSs (total)                      :: 5,198
            Genes (coding)                    :: 4,970
            CDSs (with protein)               :: 4,970
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 83 of 228
            Pseudo Genes (incomplete)         :: 171 of 228
            Pseudo Genes (internal stop)      :: 38 of 228
            Pseudo Genes (multiple problems)  :: 57 of 228
            Pseudo Genes (short protein)      :: 3 of 228
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OX030706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="131"
                     /isolation_source="Hospital Universitario Central de
                     Asturias (Oviedo)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(3899..4087,1..519))
                     /locus_tag="LQ128_RS26500"
     CDS             complement(join(3899..4087,1..519))
                     /locus_tag="LQ128_RS26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1051..1305)
                     /gene="mobC"
                     /locus_tag="LQ128_RS26505"
                     /old_locus_tag="AI2951V1_5103"
     CDS             complement(1051..1305)
                     /gene="mobC"
                     /locus_tag="LQ128_RS26505"
                     /old_locus_tag="AI2951V1_5103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1484..3643
                     /gene="mobQ"
                     /locus_tag="LQ128_RS26510"
                     /old_locus_tag="AI2951V1_5104"
     CDS             1484..3643
                     /gene="mobQ"
                     /locus_tag="LQ128_RS26510"
                     /old_locus_tag="AI2951V1_5104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023142279.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(1307..1483)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1484..1842
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..519)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1398..3640
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgatttaaaa tcatcgaggg cgataatacg ttccccagta ctgcggaatt gctggagaat
       61 ttcgtatatt cttatgctgt aacttcttga taaattagaa atatttttaa tgacgactct
      121 ggtgaattgc cccttcaact gtgtcagata aggcattacc gcgtcagaaa aagtgatttg
      181 tgctttcccc tctcccctcg cgtactgtgc ccgatactga atccagcgga actcctcgcg
      241 cttctcgtcg tccttgagaa tgatcgacct gtcccacaaa cgatcgatcg cttcctgtag
      301 ctgaacctcg gcatttttcc gccccatatc agggaatgaa tcaaaatatt ccgcagctgt
      361 aatggtcatt gtcttttgca attcaggtga agctacgtca gtaccggagt taagtcggct
      421 tatgcagagc aataagaaac gctgttcttg taaagttagc ttgtaactgg cctcaaccag
      481 cgcattggac ttataggcta tcagagggtt tgatgtcatt ttaaccacca ggccaaaaat
      541 gaggtgaaat aaaaacctat cacctttagc attggtgagc aattatattt tcacccccca
      601 ttttcctgtg aatattacct taaatctcct cacctttacc ttaaattacc tcacccaagc
      661 accttaaatt atatcacctt taccttaaat catatcacct atagcgttgt aacattttgt
      721 tttctataag gaattttggc gctaaaacaa gtattaaaaa caagatctta aaaacaagat
      781 ctaaaaactt aacgacgagc ctgtggataa cttggagtgc tgaaaacaaa aaaccaataa
      841 aaacaatgcg ttgcaaaaaa gcagaattgc aagttccgaa tacaccccct ccaaccctgt
      901 ggaaatccag tattcatgcg ggttcagcgc atatcacctc ccgaaaaacc gtgcgtatgt
      961 gctcaatcag gcgacggatt cacttccgct tcctcgccca ctcgctcgct aacgctcggt
     1021 cgctgggact gcggcgagcg gacgatacaa tcactgtcct ggcagcgctt ccagctcgtt
     1081 aatcagcggc agtatgcgcg tcacatcagc ctgtcgtgtc acctctgcct tcaggaactg
     1141 gagtaaccag gcacgcaact gcacctcctc cggctttctg acctttgcca gcaatgctgc
     1201 acccacaatg accttctgcg ctgtgtccag actcctgctc ttcgccttca ggcgctgtaa
     1261 tctggcctca gcatcagcaa tcttctgttc gagtgttctg ctcatgatgt aactccgtgc
     1321 gcgatgaaaa atcgcatttt agcgcgtcac tggtagttta aaaactgagc tggcataatg
     1381 cacggaaaat cacgaagtgc gcacttatac aatctccact ccgtttcgat tgtgtgcgcc
     1441 ctgcggggct gaaagaaaac cacaaaaagg cattacggca gaaatggcga tttatcatct
     1501 cagcatgaaa atcatctcca gaaacagcgg ctacagcgcc gttgcttctg ctgcctatcg
     1561 ttccggttca ctgatgcttg atgagcgtac cggactgact cacgactaca cccgcaaaag
     1621 cggtgtggct gaagctgtga ttctgactcc cgcgacagcc cctgcctggt gtacgaaccg
     1681 tgccgaactc tggaatgccg ttgagaaggc cgaacgccgg aagaactcac aacttgcgcg
     1741 ggaaattgaa ctcgccatcc cccgcgaact accgcaggat gccgcacggg aaaccgttct
     1801 cgcctttgtc agagaaaact ttgtcagtca gggcatgatt gctgatgtgg cgttccatca
     1861 catggataaa acaaatcccc atgcgcacat catgctgacc acgagagctg tcggacctgc
     1921 tggattcggt ggaaaggtca gggactggaa cgaccggaca catgccgaga cgtggcgtgc
     1981 atcatgggct gaccatgcga acagggcact ggcgaacgcc ggttatcagg aagagataga
     2041 ccatcgttca tacgagcgac aggggcttga gaaaacgcct ggtattcacc tcggaaagtc
     2101 tgcctgtgcg atggaaacac gcggtatcga aacagaacgc ggcgaacaga atcgtctgat
     2161 taacagactt aacctggaaa tacagatttc ccgtaccgaa ctcagaaaca gcgggctgac
     2221 agagcctgcc cgccgtgtgg ctgaccttct gaatatcgtg cttcctgaca acgcaacttc
     2281 gtacacacta cgtgacatca tcgaagcgtt gccgaaggac agtaatgcgg catggaaact
     2341 caccagtaat gccatgtcga tgatggcaga tatgcaggcg acgcgcagac gctgggaaga
     2401 gctgaacacc gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa aatcatcgcc
     2461 gttttcgtcc ggtttttccc gtattcccct gatgcaatgg gcggcaccgg aataccgtag
     2521 cgaacaggga aaaatccagt cgctgagcca gcaaatggaa aaattgcgac agcattaccg
     2581 gaaggtggag aaacaggaca ttcccgcgag tcagacggca tttgaaaaac agtggcagca
     2641 gtggggggca tccggccttg cagctctcag agtccagttg cttaaacgtg aagaggaatt
     2701 gcgccggaag gagcaggagg atacagaacg tagtcgcgcg gagcgacgag cggaacagct
     2761 caggaaaaat gacaatgccg ttatggattc aggggtactg agcgcagtcg ttacggagta
     2821 tggcgaggcc cccatgcctg gtaacgggga aatgacctgt taccttctgc ttcacaaccg
     2881 gaacggagaa tacacgctct gggggaacga gctggagaaa taccgcacca gaatctttga
     2941 gcgtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg aaatcggtca
     3001 acatccacca ctgcaacgtg tttattcctc tgcgaccttt gagcaacttc tgacgcaggt
     3061 ttgccagaca tggccgcaat atacccgcaa tctccgccag ccgaagacat ggccggagtc
     3121 tttctgtctt ggagaagacc gccagccagc catgccatca ctggcagcaa ggaaggtgga
     3181 ttttacgcag ggacgtctgc tagcgacgct gatgccggta atgagttctg tcgaccggga
     3241 aacccgccag cttcagcttt tgctggtcat gggcgtggat gactctctgg gcggtgttgt
     3301 ccggcttaat ggtacgctgt atcccgcgtt tgccgttccc tcagccgata acagccagct
     3361 ggtcatcagt gcactgaccg ataaggggtt acggtatgca gggtatggtg ttgccgtaaa
     3421 tcacgatgca gacagccaca tatcacctgc gccggaactt atggagtttc atctgaaaac
     3481 ccgtgaggca ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg atcatctgtt
     3541 ccgctcgctg ggttttaatc gtacctggga cgaatggcgg cgcgaggaag atgcacgaac
     3601 acacgccacc gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa caatattttg
     3661 ttttgtttaa catcgtgtta tgttcgtcga tgttcttcga tgttcatcga cagggatgaa
     3721 tgcaagaagc acacacataa tgcaagcata gttttgtttt tccgcccccg aaaaatgggg
     3781 gttctttttt cgctgcatta gagcgctttt gacgcaattt ttcacttcaa tagtaccttc
     3841 gcactactaa cacaaaaaaa acgcagcagg gagcgtttta ggtgccttaa aatcgattct
     3901 agatcgtcat cttgatctgt ttgtcttcct tgaagcgaaa atgcagggct acaaccgtgc
     3961 gccccttttt gatcgtctca accgttacag ccaggtcgct ttttttgttc agttcgtcaa
     4021 cacagggttt tattagatcc ctgtttaacg ttttgaaatc tttatattta ccatcaagca
     4081 tcagtga
//
