LOCUS       NZ_OW970520             4167 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 147 plasmid P6, complete sequence.
ACCESSION   NZ_OW970520
VERSION     NZ_OW970520.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082322
            Assembly: GCF_903993115.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993115.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:47:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,508
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,252
            CDSs (with protein)               :: 5,252
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 49 of 134
            Pseudo Genes (incomplete)         :: 83 of 134
            Pseudo Genes (internal stop)      :: 18 of 134
            Pseudo Genes (multiple problems)  :: 14 of 134
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970520.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="147"
                     /isolation_source="Hospital Univeristario de Bellvitge
                     (Barcelona)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P6"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            join(3693..4167,1..158)
                     /locus_tag="LQ358_RS27385"
     CDS             join(3693..4167,1..158)
                     /locus_tag="LQ358_RS27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            524..766
                     /locus_tag="LQ358_RS27390"
     CDS             524..766
                     /locus_tag="LQ358_RS27390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1112..1513)
                     /locus_tag="LQ358_RS27395"
                     /old_locus_tag="AI2763V1_5199"
     CDS             complement(1112..1513)
                     /locus_tag="LQ358_RS27395"
                     /old_locus_tag="AI2763V1_5199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1513..1704)
                     /locus_tag="LQ358_RS27400"
                     /old_locus_tag="AI2763V1_5200"
     CDS             complement(1513..1704)
                     /locus_tag="LQ358_RS27400"
                     /old_locus_tag="AI2763V1_5200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2120..2233
                     /locus_tag="LQ358_RS27405"
                     /pseudo=""
     CDS             2120..2233
                     /locus_tag="LQ358_RS27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2578..3693
                     /locus_tag="LQ358_RS27410"
                     /old_locus_tag="AI2763V1_5201"
     CDS             2578..3693
                     /locus_tag="LQ358_RS27410"
                     /old_locus_tag="AI2763V1_5201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_230297718.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGENNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          1826..2233
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 acacttatcc aacaaacgaa aaaaaagcta aggtacaagt ctatcttagc tgcaatagag
       61 aatccgactc aagaatagga atatccatga aaaatggaat aataaacaca gcagacaatt
      121 gctttgatga aataaaagat tttatttcaa aaatataaat ccaaatcaag ccagcccttc
      181 acgggctggc ttatcaccag ggtgttatta aacgccggat aacgtataga aagcatcata
      241 cactttagtt atcagcaatc tcaaacgtcc taaagacatc accagatgcc aaaacaatga
      301 ttgtgcagat ctaatgtaaa agacccagca caagatgctc ccttaagtta tattctctct
      361 attcacttaa aacaatttaa cataaaatca taattgtacg caccgctaaa acggactcag
      421 gacgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
      481 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgcg caagtgcata gcgcggaaat
      541 tcacaccatt cagccagggg aagtcctgtt tccggcgttc ttctcctccg aaaacggtga
      601 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt
      661 ttcagcacct tcaggaatcg ggttcgtcga cggccggcaa tcacgttatg gcaagtcgcc
      721 ggacgactca ccacacgatt caccggcgat tcgaatcgtg aattgaatca agagtcacca
      781 tgagcaaaaa acacccaaag tgatgaattg aatcaagagt cgattcatca taacaatatg
      841 ataataaagc gtgattcaca tatgaatcgt gggcaattcg tcagcgattc gccatagagt
      901 cgtccacgac ttggatctgc actttgtaac acatcaccgc acatttattt ttcattaacc
      961 cattgttgta aataaggaat ctgcaaaatt ttaattttgc gtggggtgtg ggtattttta
     1021 gtggtgagga gcgttcagag atctgacgat ttttgggaag gttcaggaac tccctgggat
     1081 ggccggagag gaaaactgaa gcatgacggg gtcaggattt tttccagaat ttccaccatg
     1141 gcgccgggga ttcagttaat tttgcctggt gattttcaag aaggagcatg gcctgtttca
     1201 ggctgtcaat gtgcgaatcc ttggacaaaa gtaaggtgtt caggtgttca atctcctgtt
     1261 tcagatgacg gatcacctgt tcactctggt cagcagaggc tgtatcacgc tgtccagaga
     1321 cctgtccaga atggtgttca cccttcggtt gctcaaaggg gccaaagaca cgtaaaagct
     1381 ctgaagtatc taccattttt gcattgcgct caccgctaca ggtggacagg tcacctgctt
     1441 taattaacct gtgcagtgtt gttctactct tacccgtcag tcgggcagcc tcactaatag
     1501 acactctggc catcagttac catcctgtaa tgcatccagt ctcgaggaca gtgtacggaa
     1561 aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg gcctcgacat cgagatcaag
     1621 ctcgttaagc ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag
     1681 cgctgttttc tgttgtttcg tcataagagg cctcagagcg tcagtggtca gtgtagggat
     1741 ctcctctttt ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg
     1801 agcggcagga gcatgcgtag aaacgctcga gaaaaccgag taatcgcatg gaggaaagaa
     1861 ggaaaggccg cacagcggcc gtttttccat aggctccgcc cccctgacga gcatcacgga
     1921 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1981 ccccctgatg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     2041 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
     2101 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgcctaatc
     2161 cggtaactat cgtcttgagt ccaacccgga aagacacgac aaagcgccac tggcagcagc
     2221 cactggtaac tgatttaggg agttagtctt gaagttatac gccagttaaa gctaaactaa
     2281 acgaacagtt ttggtgactg cgctcccttt aagccagtta cctcggttca tagagcgcca
     2341 actctactga acctttcgaa aaaaccgccc gccaaggcgg tttttttcgt tcacagccgg
     2401 aagcttacgg ctgctgccag ataatctcaa gaagaaagta aaaaaaatcg attaaaacac
     2461 aagcaatcaa agttaatctt tgacataaag tctagctaat cacaatgaaa gctgtacatt
     2521 ttattaaggt actaacaaat cgtatggcat ataacaaaaa cacgaacaga ggctatcatg
     2581 ataaaggaaa taagtttaaa gaacttcaaa tgcttcaatg agctatatct aaagcaatta
     2641 aagactttaa atatcatagc tggggaaaac aactacggga aaacatctat actggatgct
     2701 attttttgtt tttatgatgt aaaaaacccg gctgttttgt taaacattca agcattcaga
     2761 aaagaaatgg cagaaataaa caaaaacaag ccattttggg ttagttactt tcatgatatg
     2821 gacacatcac aaaagatgtc catagtcatt cgagatgaac gctctgaagt aacacaaaca
     2881 tatgaaacag aaacaaatca gagattagaa tcaagtttgt ctctaaacgt tgacaccgta
     2941 ctatctaacc aaaacatacc tcgtcaaact atcggttctc aggccacagt aaactcttta
     3001 aaaataaatg tgaccgagac acccaatgcg aaaaacaggg tttccaataa gcttttctct
     3061 atgcaattga aagaagatgg cgtagaaata aaatccgaaa taaaaaacga aagggatggg
     3121 attaattata aatttaagac agcaacaata ataacaacat ccagaaagat aaacaaagag
     3181 gcaacaatta caaatgtatc tagcctacta acacaaaaaa ggaaaaaaga catactagaa
     3241 aacttaaaaa aaatagatga cagaattgta gatatagcaa tatctgcgat aggtaacaat
     3301 aaggaaatat atttagacat tggatttagt gagctaaatg aaatatcaat gcttggcgag
     3361 ggtatcagtc gagcattatc ttttatcagc tcagtgctgg tacaggaaaa ctcaattatt
     3421 cttattgatg aaatcgagaa cggaatacat tactcagtaa ttaaagacat aataaaatca
     3481 ttaatttcat ctgcaaaaca aaacaacaat caaatatttg caacaactca tagtcaagat
     3541 gtgatacgag caataaacga aatagatagc aaaaatgaag atatcgctta tattagacta
     3601 ggaagagaaa agaactccct aaaaccaaca gccgtacaat tcaatatgga tgatttctcc
     3661 tactcagttg agaacgactg ggaggtaaga taatgaaaga cataaataat agcaaaaaga
     3721 tcattttcgt tgagggtgat gatgattttc attttctatg caacctactt gaaatacaag
     3781 gcattaccga tgtttttgtc gaaaaaataa atggaaaatc aaaattaaaa caagcactta
     3841 gagcattcaa aaacattgat tctttcgatg aaaaagaaag tatttttatt atattagatg
     3901 ctgacacatc attttctgac acagaaagat caataattgc cacattgcga gaactacaaa
     3961 tctcctcccc cagctcgcat aacgagatag ctatgaatgg caatacaaaa atttctttct
     4021 ttataatgcc aggaaaagac aagcaaggtg ccattgaaga tctgataatt gaacacgcaa
     4081 gcactcgagc gttatatgaa tacataatag aatttttcga aaaaattaaa aataataaag
     4141 atgacatctc agcgagtgac aaagagt
//
