LOCUS       NZ_OW970499             4167 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 147 plasmid P7, complete sequence.
ACCESSION   NZ_OW970499
VERSION     NZ_OW970499.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082332
            Assembly: GCF_903993145.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993145.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:56:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,547
            CDSs (total)                      :: 5,426
            Genes (coding)                    :: 5,289
            CDSs (with protein)               :: 5,289
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 49 of 137
            Pseudo Genes (incomplete)         :: 87 of 137
            Pseudo Genes (internal stop)      :: 17 of 137
            Pseudo Genes (multiple problems)  :: 14 of 137
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970499.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="147"
                     /isolation_source="Hospital Univeristario de Bellvitge
                     (Barcelona)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            join(3905..4167,1..370)
                     /locus_tag="LQV37_RS27580"
     CDS             join(3905..4167,1..370)
                     /locus_tag="LQV37_RS27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            736..978
                     /locus_tag="LQV37_RS27585"
     CDS             736..978
                     /locus_tag="LQV37_RS27585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1324..1725)
                     /locus_tag="LQV37_RS27590"
                     /old_locus_tag="AI2996V1_5236"
     CDS             complement(1324..1725)
                     /locus_tag="LQV37_RS27590"
                     /old_locus_tag="AI2996V1_5236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1725..1916)
                     /locus_tag="LQV37_RS27595"
                     /old_locus_tag="AI2996V1_5237"
     CDS             complement(1725..1916)
                     /locus_tag="LQV37_RS27595"
                     /old_locus_tag="AI2996V1_5237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2332..2445
                     /locus_tag="LQV37_RS27600"
                     /pseudo=""
     CDS             2332..2445
                     /locus_tag="LQV37_RS27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2790..3905
                     /locus_tag="LQV37_RS27605"
                     /old_locus_tag="AI2996V1_5238"
     CDS             2790..3905
                     /locus_tag="LQV37_RS27605"
                     /old_locus_tag="AI2996V1_5238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          2038..2445
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 acaaatctcc tcccccagct cgcataacga gatagctatg aatggcaata caaaaatttc
       61 tttctttata atgccaggaa aagacaagca aggtgccatt gaagatctga taattgaaca
      121 cgcaagcact cgagcgttat atgaatacat aatagaattt ttcgaaaaaa ttaaaaataa
      181 taaagatgac atctcagcga gtgacaaaga gtacacttat ccaacaaacg aaaaaaaagc
      241 taaggtacaa gtctatctta gctgcaatag agaatccgac tcaagaatag gaatatccat
      301 gaaaaatgga ataataaaca cagcagacaa ttgctttgat gaaataaaag attttatttc
      361 aaaaatataa atccaaatca agccagccct tcacgggctg gcttatcacc agggtgttat
      421 taaacgccgg ataacgtata gaaagcatca tacactttag ttatcagcaa tctcaaacgt
      481 cctaaagaca tcaccagatg ccaaaacaat gattgtgcag atctaatgta aaagacccag
      541 cacaagatgc tcccttaagt tatattctct ctattcactt aaaacaattt aacataaaat
      601 cataattgta cgcaccgcta aaacggactc aggacgtttc tgacgggaaa ccggtaaaac
      661 aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt acggcatgca
      721 tgagtatgcg cgcaagtgca tagcgcggaa attcacacca ttcagccagg ggaagtcctg
      781 tttccggcgt tcttctcctc cgaaaacggt gaacggggac gcaatttatt gcgtggccgt
      841 gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac cttcaggaat cgggttcgtc
      901 gacggccggc aatcacgtta tggcaagtcg ccggacgact caccacacga ttcaccggcg
      961 attcgaatcg tgaattgaat caagagtcac catgagcaaa aaacacccaa agtgatgaat
     1021 tgaatcaaga gtcgattcat cataacaata tgataataaa gcgtgattca catatgaatc
     1081 gtgggcaatt cgtcagcgat tcgccataga gtcgtccacg acttggatct gcactttgta
     1141 acacatcacc gcacatttat ttttcattaa cccattgttg taaataagga atctgcaaaa
     1201 ttttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgacg
     1261 atttttggga aggttcagga actccctggg atggccggag aggaaaactg aagcatgacg
     1321 gggtcaggat tttttccaga atttccacca tggcgccggg gattcagtta attttgcctg
     1381 gtgattttca agaaggagca tggcctgttt caggctgtca atgtgcgaat ccttggacaa
     1441 aagtaaggtg ttcaggtgtt caatctcctg tttcagatga cggatcacct gttcactctg
     1501 gtcagcagag gctgtatcac gctgtccaga gacctgtcca gaatggtgtt cacccttcgg
     1561 ttgctcaaag gggccaaaga cacgtaaaag ctctgaagta tctaccattt ttgcattgcg
     1621 ctcaccgcta caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact
     1681 cttacccgtc agtcgggcag cctcactaat agacactctg gccatcagtt accatcctgt
     1741 aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc
     1801 tcacaaaggt cggcctcgac atcgagatca agctcgttaa gcttctccag caggagcaga
     1861 gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt cgtcataaga
     1921 ggcctcagag cgtcagtggt cagtgtaggg atctcctctt ttccgcttcc tcgctcactc
     1981 agtcgctacg ctcctgccgt tcggctgcgg cgagcggcag gagcatgcgt agaaacgctc
     2041 gagaaaaccg agtaatcgca tggaggaaag aaggaaaggc cgcacagcgg ccgtttttcc
     2101 ataggctccg cccccctgac gagcatcacg gaatctgacg ctcaaatcag tggtggcgaa
     2161 acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc gtgcgctctc
     2221 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     2281 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     2341 ccccccgttc agtccgactg ctgcgcctaa tccggtaact atcgtcttga gtccaacccg
     2401 gaaagacacg acaaagcgcc actggcagca gccactggta actgatttag ggagttagtc
     2461 ttgaagttat acgccagtta aagctaaact aaacgaacag ttttggtgac tgcgctccct
     2521 ttaagccagt tacctcggtt catagagcgc caactctact gaacctttcg aaaaaaccgc
     2581 ccgccaaggc ggtttttttc gttcacagcc ggaagcttac ggctgctgcc agataatctc
     2641 aagaagaaag taaaaaaaat cgattaaaac acaagcaatc aaagttaatc tttgacataa
     2701 agtctagcta atcacaatga aagctgtaca ttttattaag gtactaacaa atcgtatggc
     2761 atataacaaa aacacgaaca gaggctatca tgataaagga aataagttta aagaacttca
     2821 aatgcttcaa tgagctatat ctaaagcaat taaagacttt aaatatcata gctgggaaaa
     2881 acaactacgg gaaaacatct atactggatg ctattttttg tttttatgat gtaaaaaacc
     2941 cggctgtttt gttaaacatt caagcattca gaaaagaaat ggcagaaata aacaaaaaca
     3001 agccattttg ggttagttac tttcatgata tggacacatc acaaaagatg tccatagtca
     3061 ttcgagatga acgctctgaa gtaacacaaa catatgaaac agaaacaaat cagagattag
     3121 aatcaagttt gtctctaaac gttgacaccg tactatctaa ccaaaacata cctcgtcaaa
     3181 ctatcggttc tcaggccaca gtaaactctt taaaaataaa tgtgaccgag acacccaatg
     3241 cgaaaaacag ggtttccaat aagcttttct ctatgcaatt gaaagaagat ggcgtagaaa
     3301 taaaatccga aataaaaaac gaaagggatg ggattaatta taaatttaag acagcaacaa
     3361 taataacaac atccagaaag ataaacaaag aggcaacaat tacaaatgta tctagcctac
     3421 taacacaaaa aaggaaaaaa gacatactag aaaacttaaa aaaaatagat gacagaattg
     3481 tagatatagc aatatctgcg ataggtaaca ataaggaaat atatttagac attggattta
     3541 gtgagctaaa tgaaatatca atgcttggcg agggtatcag tcgagcatta tcttttatca
     3601 gctcagtgct ggtacaggaa aactcaatta ttcttattga tgaaatcgag aacggaatac
     3661 attactcagt aattaaagac ataataaaat cattaatttc atctgcaaaa caaaacaaca
     3721 atcaaatatt tgcaacaact catagtcaag atgtgatacg agcaataaac gaaatagata
     3781 gcaaaaatga agatatcgct tatattagac taggaagaga aaagaactcc ctaaaaccaa
     3841 cagccgtaca attcaatatg gatgatttct cctactcagt tgagaacgac tgggaggtaa
     3901 gataatgaaa gacataaata atagcaaaaa gatcattttc gttgagggtg atgatgattt
     3961 tcattttcta tgcaacctac ttgaaataca aggcattacc gatgtttttg tcgaaaaaat
     4021 aaatggaaaa tcaaaattaa aacaagcact tagagcattc aaaaacattg attctttcga
     4081 tgaaaaagaa agtattttta ttatattaga tgctgacaca tcattttctg acacagaaag
     4141 atcaataatt gccacattgc gagaact
//
