LOCUS       NZ_OW970491             4167 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 147 plasmid P5, complete sequence.
ACCESSION   NZ_OW970491
VERSION     NZ_OW970491.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082317
            Assembly: GCF_903993165.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993165.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:56:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,573
            CDSs (total)                      :: 5,451
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 50 of 123
            Pseudo Genes (incomplete)         :: 74 of 123
            Pseudo Genes (internal stop)      :: 16 of 123
            Pseudo Genes (multiple problems)  :: 15 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970491.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="147"
                     /isolation_source="Hospital Universitario Lucus Augusti
                     (Lugo)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            join(3704..4167,1..169)
                     /locus_tag="LQV31_RS27710"
     CDS             join(3704..4167,1..169)
                     /locus_tag="LQV31_RS27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            535..777
                     /locus_tag="LQV31_RS27715"
     CDS             535..777
                     /locus_tag="LQV31_RS27715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1123..1524)
                     /locus_tag="LQV31_RS27720"
                     /old_locus_tag="AI2830V1_5245"
     CDS             complement(1123..1524)
                     /locus_tag="LQV31_RS27720"
                     /old_locus_tag="AI2830V1_5245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1524..1715)
                     /locus_tag="LQV31_RS27725"
                     /old_locus_tag="AI2830V1_5246"
     CDS             complement(1524..1715)
                     /locus_tag="LQV31_RS27725"
                     /old_locus_tag="AI2830V1_5246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2131..2244
                     /locus_tag="LQV31_RS27730"
                     /pseudo=""
     CDS             2131..2244
                     /locus_tag="LQV31_RS27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2589..3704
                     /locus_tag="LQV31_RS27735"
                     /old_locus_tag="AI2830V1_5247"
     CDS             2589..3704
                     /locus_tag="LQV31_RS27735"
                     /old_locus_tag="AI2830V1_5247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          1837..2244
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 tgacaaagag tacacttatc caacaaacga aaaaaaagct aaggtacaag tctatcttag
       61 ctgcaataga gaatccgact caagaatagg aatatccatg aaaaatggaa taataaacac
      121 agcagacaat tgctttgatg aaataaaaga ttttatttca aaaatataaa tccaaatcaa
      181 gccagccctt cacgggctgg cttatcacca gggtgttatt aaacgccgga taacgtatag
      241 aaagcatcat acactttagt tatcagcaat ctcaaacgtc ctaaagacat caccagatgc
      301 caaaacaatg attgtgcaga tctaatgtaa aagacccagc acaagatgct cccttaagtt
      361 atattctctc tattcactta aaacaattta acataaaatc ataattgtac gcaccgctaa
      421 aacggactca ggacgtttct gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa
      481 cccgccagaa acgcgcaggc tctgttttta cggcatgcat gagtatgcgc gcaagtgcat
      541 agcgcggaaa ttcacaccat tcagccaggg gaagtcctgt ttccggcgtt cttctcctcc
      601 gaaaacggtg aacggggacg caatttattg cgtggccgtg aagcgttgcc ggcgcagccg
      661 gaaaaccggt tttcagcacc ttcaggaatc gggttcgtcg acggccggca atcacgttat
      721 ggcaagtcgc cggacgactc accacacgat tcaccggcga ttcgaatcgt gaattgaatc
      781 aagagtcacc atgagcaaaa aacacccaaa gtgatgaatt gaatcaagag tcgattcatc
      841 ataacaatat gataataaag cgtgattcac atatgaatcg tgggcaattc gtcagcgatt
      901 cgccatagag tcgtccacga cttggatctg cactttgtaa cacatcaccg cacatttatt
      961 tttcattaac ccattgttgt aaataaggaa tctgcaaaat tttaattttg cgtggggtgt
     1021 gggtattttt agtggtgagg agcgttcaga gatctgacga tttttgggaa ggttcaggaa
     1081 ctccctggga tggccggaga ggaaaactga agcatgacgg ggtcaggatt ttttccagaa
     1141 tttccaccat ggcgccgggg attcagttaa ttttgcctgg tgattttcaa gaaggagcat
     1201 ggcctgtttc aggctgtcaa tgtgcgaatc cttggacaaa agtaaggtgt tcaggtgttc
     1261 aatctcctgt ttcagatgac ggatcacctg ttcactctgg tcagcagagg ctgtatcacg
     1321 ctgtccagag acctgtccag aatggtgttc acccttcggt tgctcaaagg ggccaaagac
     1381 acgtaaaagc tctgaagtat ctaccatttt tgcattgcgc tcaccgctac aggtggacag
     1441 gtcacctgct ttaattaacc tgtgcagtgt tgttctactc ttacccgtca gtcgggcagc
     1501 ctcactaata gacactctgg ccatcagtta ccatcctgta atgcatccag tctcgaggac
     1561 agtgtacgga aaagatgctc cgcatcatcg tggagtttct cacaaaggtc ggcctcgaca
     1621 tcgagatcaa gctcgttaag cttctccagc aggagcagag actgattctg gatgaattta
     1681 gccatgttca gcgctgtttt ctgttgtttc gtcataagag gcctcagagc gtcagtggtc
     1741 agtgtaggga tctcctcttt tccgcttcct cgctcactca gtcgctacgc tcctgccgtt
     1801 cggctgcggc gagcggcagg agcatgcgta gaaacgctcg agaaaaccga gtaatcgcat
     1861 ggaggaaaga aggaaaggcc gcacagcggc cgtttttcca taggctccgc ccccctgacg
     1921 agcatcacgg aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     1981 accaggcgtt tccccctgat ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     2041 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     2101 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc
     2161 tgcgcctaat ccggtaacta tcgtcttgag tccaacccgg aaagacacga caaagcgcca
     2221 ctggcagcag ccactggtaa ctgatttagg gagttagtct tgaagttata cgccagttaa
     2281 agctaaacta aacgaacagt tttggtgact gcgctccctt taagccagtt acctcggttc
     2341 atagagcgcc aactctactg aacctttcga aaaaaccgcc cgccaaggcg gtttttttcg
     2401 ttcacagccg gaagcttacg gctgctgcca gataatctca agaagaaagt aaaaaaaatc
     2461 gattaaaaca caagcaatca aagttaatct ttgacataaa gtctagctaa tcacaatgaa
     2521 agctgtacat tttattaagg tactaacaaa tcgtatggca tataacaaaa acacgaacag
     2581 aggctatcat gataaaggaa ataagtttaa agaacttcaa atgcttcaat gagctatatc
     2641 taaagcaatt aaagacttta aatatcatag ctgggaaaaa caactacggg aaaacatcta
     2701 tactggatgc tattttttgt ttttatgatg taaaaaaccc ggctgttttg ttaaacattc
     2761 aagcattcag aaaagaaatg gcagaaataa acaaaaacaa gccattttgg gttagttact
     2821 ttcatgatat ggacacatca caaaagatgt ccatagtcat tcgagatgaa cgctctgaag
     2881 taacacaaac atatgaaaca gaaacaaatc agagattaga atcaagtttg tctctaaacg
     2941 ttgacaccgt actatctaac caaaacatac ctcgtcaaac tatcggttct caggccacag
     3001 taaactcttt aaaaataaat gtgaccgaga cacccaatgc gaaaaacagg gtttccaata
     3061 agcttttctc tatgcaattg aaagaagatg gcgtagaaat aaaatccgaa ataaaaaacg
     3121 aaagggatgg gattaattat aaatttaaga cagcaacaat aataacaaca tccagaaaga
     3181 taaacaaaga ggcaacaatt acaaatgtat ctagcctact aacacaaaaa aggaaaaaag
     3241 acatactaga aaacttaaaa aaaatagatg acagaattgt agatatagca atatctgcga
     3301 taggtaacaa taaggaaata tatttagaca ttggatttag tgagctaaat gaaatatcaa
     3361 tgcttggcga gggtatcagt cgagcattat cttttatcag ctcagtgctg gtacaggaaa
     3421 actcaattat tcttattgat gaaatcgaga acggaataca ttactcagta attaaagaca
     3481 taataaaatc attaatttca tctgcaaaac aaaacaacaa tcaaatattt gcaacaactc
     3541 atagtcaaga tgtgatacga gcaataaacg aaatagatag caaaaatgaa gatatcgctt
     3601 atattagact aggaagagaa aagaactccc taaaaccaac agccgtacaa ttcaatatgg
     3661 atgatttctc ctactcagtt gagaacgact gggaggtaag ataatgaaag acataaataa
     3721 tagcaaaaag atcattttcg ttgagggtga tgatgatttt cattttctat gcaacctact
     3781 tgaaatacaa ggcattaccg atgtttttgt cgaaaaaata aatggaaaat caaaattaaa
     3841 acaagcactt agagcattca aaaacattga ttctttcgat gaaaaagaaa gtatttttat
     3901 tatattagat gctgacacat cattttctga cacagaaaga tcaataattg ccacattgcg
     3961 agaactacaa atctcctccc ccagctcgca taacgagata gctatgaatg gcaatacaaa
     4021 aatttctttc tttataatgc caggaaaaga caagcaaggt gccattgaag atctgataat
     4081 tgaacacgca agcactcgag cgttatatga atacataata gaatttttcg aaaaaattaa
     4141 aaataataaa gatgacatct cagcgag
//
