LOCUS       NZ_OW970490             4715 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 147 plasmid P4, complete sequence.
ACCESSION   NZ_OW970490
VERSION     NZ_OW970490.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082317
            Assembly: GCF_903993165.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993165.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:56:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,573
            CDSs (total)                      :: 5,451
            Genes (coding)                    :: 5,328
            CDSs (with protein)               :: 5,328
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 50 of 123
            Pseudo Genes (incomplete)         :: 74 of 123
            Pseudo Genes (internal stop)      :: 16 of 123
            Pseudo Genes (multiple problems)  :: 15 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970490.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="147"
                     /isolation_source="Hospital Universitario Lucus Augusti
                     (Lugo)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            join(4441..4715,1..682)
                     /locus_tag="LQV31_RS27690"
     CDS             join(4441..4715,1..682)
                     /locus_tag="LQV31_RS27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            666..1532
                     /locus_tag="LQV31_RS27695"
                     /old_locus_tag="AI2830V1_5242"
     CDS             666..1532
                     /locus_tag="LQV31_RS27695"
                     /old_locus_tag="AI2830V1_5242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            1514..2182
                     /locus_tag="LQV31_RS27700"
                     /old_locus_tag="AI2830V1_5243"
     CDS             1514..2182
                     /locus_tag="LQV31_RS27700"
                     /old_locus_tag="AI2830V1_5243"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_223195384.1"
                     /translation="MGYIQMIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCS
                     EWNMKILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYN
                     KSGVLLTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFD
                     LNDENKEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS
                     "
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(2404..2517)
                     /locus_tag="LQV31_RS27900"
     CDS             complement(2404..2517)
                     /locus_tag="LQV31_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427893.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(3171..3365)
                     /locus_tag="LQV31_RS27705"
                     /old_locus_tag="AI2830V1_5244"
     CDS             complement(3171..3365)
                     /locus_tag="LQV31_RS27705"
                     /old_locus_tag="AI2830V1_5244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3014..3098
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          3402..3500
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          3625..3880
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          complement(2446..3003)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.821"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttttttgtt aaaggtaacg atggttctcc ggaaaatgat atagtgtgct ctacgtatcc
       61 atatggatac ttatcctatt tatccgctat ggattggcat aacatcacag ataaaatacc
      121 caagataatc cactttacga catgtcgttc cgaacagtgg aagaataagt tctatcatga
      181 ttttcaaaag gataatcctg atatagacga agccaaacaa ttcatgccta gatttccaag
      241 aaattctgag gtcaatggaa taaaaattat cgttacacaa gaaacaaatt atgttgagcc
      301 aatgaaggct cgtaattccc ctgtcagggt atcatctatc ggtaagacct ttatggatat
      361 gaccaggcgg cctgattact gtggtggtga ggagcatgta ttggaatcat ttattgagca
      421 cggtaaaaaa tatgcacctc tgattataaa ggaagtcgat aagcttggcc gtcatattga
      481 caaggtaagg gttggattca ttttggatag aattattggg gttaataata aaaaaataag
      541 cgaatggaaa caagaggcgg gtaaatacag aggtagttcg aaagtgctat ctccaaagag
      601 tccattctca ccttactttg atgaagaatg gtttatttct attaatgttg atatggttaa
      661 gtcttatgga aaaagaaatt aataattgga tcgaagagtc tggtgggaag tacaggttga
      721 tgcgccaggc tgtacatata atacttaaat ctatttctat agaggagagt ttgaatgaaa
      781 tgatggttat gaagggaggg actcttcttg gtgttaagca tggtagtgat agatacacaa
      841 aggatattga tttttctacc gaaaaaaaac tttctgaaat tgacttgggg gattttttca
      901 ataggtttaa tgactcaatg gatgttgctg aggctgaatt gggatatggt attaaatgta
      961 aagttcaatc tcataaaatt caacctaatg cacaaggaac atttccaact gtacagataa
     1021 atatagcctt ttgcgatcgt tctaatgcgt ctgcaatgaa aaggcttgaa tctaatcaat
     1081 caccttcagt gataaatatc gattttagct ttaacgaaaa aacgtatgat tttgactttt
     1141 tatcattaga tggtgatgat gacaatgata gcgtaaagac ctacagctta actgatttga
     1201 tggctgaaaa atatcgatcc gttttgcaac aaaaagttag agagagaaat cgagagcaag
     1261 atatatatga tatcaattat cttttaggta aatatgagtt ttctcggcaa gataagtata
     1321 aaatactgga gtctctttta aaaaagagtg aaggtaaaca attggataat ctcttaaacg
     1381 ttaaaggtat aagggatgta gaaattatcg agcgttccag tcagcgatat caggatttat
     1441 catccaccgt taaatcatta cctctttttg aagatgctta tgaaaaagtt gcctgtttct
     1501 atgaaagtct tccgtgggat atattcaaat gatagcgttc agttttagtg atattggtaa
     1561 cagatgtgaa aacttagact tattatctac gataaattgt caaaatgatg tgtttggaat
     1621 ggttattatg gatgggtatt cctgctctga gtggaatatg aaaattctct ctggtaggtt
     1681 agataagtgc gtgttttctg gtgatctgaa taaagatatt attgaaccat gcaagtcagt
     1741 tcctgttaag gctgcagtat gtttcctttt taaaaataat acgcacctcc atatatataa
     1801 cgcaggagat tgccgtgtat ataacaaaag tggagtcctt ctaacagagg ataactcggt
     1861 tgcctggaaa agattaaagg aaaatggaat cgataataaa aaaatacctc acctcgtttg
     1921 ttattgccca gacagaaatt atctaacatc atttgttgat ttctgctctg atgagataat
     1981 tttcaatcat tcagtttttg atttaaatga tgaaaacaaa gaggttattt tatgtactga
     2041 tgggttttgg gagcatttta atgatggtac tgttattgac ctcatgaaag ataacccaga
     2101 atctaacttg gctaaattta ctaaaaatat cccgggtaac gcagagaatc atacggtttg
     2161 ctacgttaga ttcccttctt gatttcgtgt tttttgaaga agctattgag atcttcgtgt
     2221 catatagata atctcttttc tgtaaaagaa aaaaaccgcc tttgcggggc ggttttctct
     2281 taggttcaaa gagtttgcgg ctcgaagaac caaggtaact ggcttggagg agcgcagtca
     2341 ccaaaacctg ttctttcagt ttagccttaa ctggcgcatg acttcaagac taactcctct
     2401 aaatcagtta ccagtggctg ctgccagtgg cgttaaggcg tgtcttaccg ggttggactc
     2461 aagacgatag ttaccggata aggcgcagca gtcgggctga acggggggtt cgtgcacaca
     2521 gcccagcttg gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga
     2581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2641 aacaggagag cgcacgaggg agccaccagg gggaaacgtc tggtatcttt aagtcctgtc
     2701 gggtttcgcc acctctgact tgagcgtcga tttctgtgat gctcgtcagg ggggcggagc
     2761 ctatggaaaa acgcctgcgg cgcggccttc ttcctgttct tggtcttttg ctcacatgtt
     2821 ctttccggcc ttatcccctg attctgtgga taaccgtgct gccgtttttg tgtaagtcag
     2881 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaaa
     2941 gcgcctggac gcgcattttc tccttacgca tctgtgcgga atttcacacc cggcatgggg
     3001 cacttttcat acaattcgca ctgatgccgc atagttaagc cagtatacac tccgctatcg
     3061 ctacgtgact gggtcagggc ttcgccccga cacccgctga cgcgccctgg cgggcttgtc
     3121 tgctgccgaa aacgctcgcg gacaagccgt gagcgcctcc gtcagcttcg ttatgttcct
     3181 gtttcacttt cggtctcaaa gcgaacctgg atgctgttct ggagttcttc cgcgagttcg
     3241 tgcagtcgtt cgcacatggc cgcctgttcg tcggcatcca gagcatccag tttttcaagc
     3301 agtgtcaggc tctggctttt gataaatcgc gccatattca gcgcagtttg ttgctgtttg
     3361 ttcaccttcc gttgttctcc gtcagtaccg ggtgtcgggt ggaccctgac caggtggtaa
     3421 ttgtaagacg ttgcgggcga cggtattaca attgcacatc ctgtcctgtt tttttaggac
     3481 gtatcatggc acagcatatc aactgataaa taatccctgt gcagtgcagc cgctgttgca
     3541 taaaatttcg ctctgcgtcg agtgtttctt aatcaataat tccgctgata ctccggaaaa
     3601 acgcgagtct gttctcatgc ctgaaatgcc cacaccccac gcaaaaacaa ttttttgctg
     3661 atttttcttt ataaatagag agttatgaca aattagttct tcttgctctc tttgtgatat
     3721 ttaaaaaagc ggtgtcggcg cggtgttgta gctgcgccaa caccgctttt taggggtggt
     3781 actgactatt ttcataaaaa acatcatttt atattagggg ggctgctagc ggcgcggtgt
     3841 gtttttttac aggacgcccc tgggggcgct gctaggggtg tctgttcaga tatgtagcaa
     3901 tgagtcaggg attcacgaac tgaaaatgtc cgaaaatgtg tctcatgggc actgtatgcg
     3961 cccaaaccga tcgtcattca ctttcatgca tagctatgca gtgagctgaa agcgatcctg
     4021 acgcattttt ccggtttacc ccggggagaa catctctttt tgcggtgttc gcggcagaat
     4081 cgcgttcagc gcgttttagc ggtgcgcgta atgcgacgtt atggtaaatg acgctgaaaa
     4141 atgatctttt acgctcatcg tttatgagca acagatgtcc taatcatgga tttgaactat
     4201 aaccggcgct gaattgatcg catgaggtgt tatgaaaacg gtgatataag taaacttttt
     4261 cttgacttcc tacttattat cctataaaat aaatccttag gatttatttt ataggatatc
     4321 aaaaatataa aaatggcaaa tgcttgtttg ttattatttt tttcttgaca atcaatctct
     4381 aatagaatat acgtccttag gacgtatatt ctattagaga ctaaaccgaa aaggttgaat
     4441 atgtatctta tcgaagaagc gctaacgtct tggttgttga atgaatataa aaaaccagtc
     4501 ataaccaaag aagaaatctc tttgcagatt tacctttttt atttaaaaaa agaatataat
     4561 ggtgagagga tatatcaaat tagaaagaac aaagcaggac ctaatgaatt tgaaaaaaat
     4621 atattaaaat tattaaagtc aggggtttta tcaagagttg aaagtaacga taagcatcat
     4681 gcttaccccc atttaattgg cggtttctat gaggt
//
