LOCUS       NZ_OW970451             3479 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 101 plasmid P7, complete sequence.
ACCESSION   NZ_OW970451
VERSION     NZ_OW970451.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082320
            Assembly: GCF_903993205.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993205.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:46:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,497
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 54 of 132
            Pseudo Genes (incomplete)         :: 81 of 132
            Pseudo Genes (internal stop)      :: 19 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970451.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="101"
                     /isolation_source="Hospital Universitario Ramon y Cajal
                     (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            12..203
                     /locus_tag="LQV08_RS27930"
                     /old_locus_tag="AI2898V1_5305"
     CDS             12..203
                     /locus_tag="LQV08_RS27930"
                     /old_locus_tag="AI2898V1_5305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_011977837.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            203..604
                     /locus_tag="LQV08_RS27935"
                     /old_locus_tag="AI2898V1_5306"
     CDS             203..604
                     /locus_tag="LQV08_RS27935"
                     /old_locus_tag="AI2898V1_5306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_011977838.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGSFEQPKGEHLSGQVSEQRDTAFADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1387..2064)
                     /locus_tag="LQV08_RS27940"
     CDS             complement(1387..2064)
                     /locus_tag="LQV08_RS27940"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_117058735.1"
                     /translation="MIYKEKKINRNQIPAILASRWGYSILEEAGWTTVPNLLLEKQMVL
                     ELSNTEMLLLIHLISFIHFADTPAFPSISTLAGRINQHPRTVQRTINKLISKGVLKRKI
                     RAKSVNDKGLSNLYSFEPLIEKLLISISNNKDQYEELQKEQGERDLKTVDQLVRIAAAG
                     GGLRLSASTKTVDQLVRIAAAAGNSITPNKAKLVLFDLESKTVDELVRIAAAGKGCVLF
                     ELE"
                     /name="helix-turn-helix domain-containing protein"
     gene            2257..2487
                     /locus_tag="LQV08_RS27945"
                     /old_locus_tag="AI2898V1_5307"
     CDS             2257..2487
                     /locus_tag="LQV08_RS27945"
                     /old_locus_tag="AI2898V1_5307"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_011977835.1"
                     /translation="MTTAIGKWGNGAALRIPQPFMKQLGLSIGDVVNFRVTERDLVITK
                     AGPTLEELLGQCTAENRHHEYLCDSQGKEML"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2484..2822
                     /gene="mazF"
                     /locus_tag="LQV08_RS27950"
     CDS             2484..2822
                     /gene="mazF"
                     /locus_tag="LQV08_RS27950"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="WP_011977836.1"
                     /translation="MTTYCPARGDVILLDFNPQSGHEQAGKRPALVVSDDLFNQVTGFA
                     VVCPITNQIKGYPFEVPVDGTTKTTGVILADQVKSLDWKARAARTVDSVSGETVTTVVD
                     MVSKIIKA"
                     /name="mazF"
     gene            complement(2953..3086)
                     /locus_tag="LQV08_RS27955"
                     /pseudo=""
     CDS             complement(2953..3086)
                     /locus_tag="LQV08_RS27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(2986..3443)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.5"
                     /coverage="90.0"
                     /evalue="1.69e-147"
ORIGIN
        1 tgaggcttct tatgacgaaa caacagaaaa cagcgctgaa catggctaaa ttcatccagc
       61 atcagtctct gctcctgctg gagaagctta acgagcttga tcttgatgct gaggccgacc
      121 tttgtgagaa actccatgat gatgcggagc atcttttccg tacactgtcc tcgagactgg
      181 atgcattaca ggatggtaac tgatggccag agtgtctatt agtgaggcag cccgattgac
      241 gggtaagagt agaacaacac tgcacaggtt aattaaagca ggtgacctgt ccacctgtag
      301 cggtgaacgt aatgcaaaaa tggtagatac gtcagagctt ttgcgtgtct ttggttcctt
      361 tgagcaacct aagggtgaac atctttctgg acaggtctct gaacagcgtg atacagcctt
      421 tgctgaccag aatgaacagg ttattcgtca tctgaaacag gaaattgaac acctgaacac
      481 cttacttttg tccaaggatt cgcacattga cagcctgaaa caggccatgc tccttcttga
      541 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaagtc
      601 ctgacaccgt catgcttcag ttttcctctc cggccattcc ggggagttcc tgaaccttcc
      661 caaaaatcgt cagatctctg aacgcacctc accacttaaa atacccacac accacgcaaa
      721 attaaaattt tgcagataac tcattaatat cagtttgtta ggtgttatta aattacgaat
      781 tattaccttc tgtaaggtaa atccaagtcg ccggcaagtc gtatacgact tgccggcgac
      841 ttgtcataga gtcatcgaca agtcactcca gactatcagt aacttatgac gacttgactc
      901 tagagttgag tcgttcattt ggtgtttttt tgaccttggt gactctagag tcaagtcacg
      961 agtcgaatcg ccggtgaatc gtgtgctaag tcgtccggtg acttgccatc acgtcattgc
     1021 cggctgtcgc tgaactggat tcttgaacgt gctgaaaacg ggttttccgg ctgcgccggc
     1081 aacgcctcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tcagaaaaac
     1141 gccggaaaca ggacttaccc tggctgaatg gcatgaattc ccgcgctatg cacttgcatg
     1201 catactcatg tatgccgtaa aaacagcccc tgcgcgtttc tggcaggttt tcgggtggtt
     1261 tgttgcctgt tttgccggtt tcccgtcaga aacgccctga agccgtttta gcggtgcacg
     1321 taatagggct ttatgttaaa ttaagactgg tttgatgttt aaacttatag tgatcttttt
     1381 agtgtgttac tccaattcga atagtacaca cccctttcct gctgcagcaa ttcgaactag
     1441 ctcatcaaca gtcttggact caagatcaaa cagaactaac tttgctttat tgggggttat
     1501 cgagttacct gcggctgcgg caatccgcac gagctgatcc actgtttttg tggatgcact
     1561 taaacgtaaa ccacctcctg ctgcagcaat ccgaactaat tgatctactg ttttcaagtc
     1621 tctttctcct tgttctttct gaagttcttc atactggtct ttgttgttac taattgatat
     1681 taatagcttt tctatcaaag gttcaaagct gtaaagattg ctcaagcctt tgtcattgac
     1741 tgattttgcg cggatttttc tcttcaagac ccctttagat attaatttgt ttatggttcg
     1801 ctgcacggtt cttgggtgtt ggttgatcct acctgctagt gtagaaatgc ttgggaatgc
     1861 tggggtgtct gcaaagtgaa tgaaagagat tagatgaatg agtaacagca tttcggtgtt
     1921 actcaattct agaaccatct gtttttcaag caataaatta ggaacagttg tccaccctgc
     1981 ttcctctagg atgctatatc cccacctgct tgctaagatc gctggtattt ggttgcggtt
     2041 tatttttttt tctttgtaaa tcaatttgtt atcgccactt agttaaaaaa aatttttttt
     2101 ttgccattgg taactactta aacaaataag tatttatttg tttaagtagt tacccctttt
     2161 tagggtggca ttggtgccac ccttcaaact gaactcaaag actatacaat acaaatattg
     2221 tttacacatt gtttatccat ctgctttgga ggtattatga ctacggctat tggtaaatgg
     2281 ggtaatggcg ctgctttgcg tatcccgcag ccgttcatga agcagcttgg gcttagtatc
     2341 ggtgatgttg ttaatttccg cgtcactgaa agggatttag taattactaa agctggcccg
     2401 acgcttgaag aactgctcgg acagtgcact gcagaaaatc gccatcacga gtatctatgt
     2461 gactctcaag gtaaggagat gctgtgacga catattgtcc agccagaggc gatgtcattc
     2521 ttttagattt taaccctcaa tcagggcatg agcaggcagg gaaaaggcct gctcttgtcg
     2581 tttctgatga tttgtttaac caagtcactg ggtttgctgt tgtttgcccc ataactaatc
     2641 agataaaagg gtaccctttt gaagttcctg ttgatgggac tactaaaaca acaggggtta
     2701 tcttggctga tcaagtgaag tctttagact ggaaggccag agcggcacgt actgttgata
     2761 gcgtttccgg cgagacagta actacagttg ttgatatggt ttcaaaaatt attaaagcat
     2821 aaaaaaagac cggcataagc cggtcttttc aggccgaaaa tgttaccagc attttcggcc
     2881 ttcggtgact gctctaaagg ggaacagtaa ccaatattgt ttatcaagtg taactgctta
     2941 ctgttacagc aatcaagatt ttctttcccc cgtagagcga ccgatggcta ctgccaatgg
     3001 cgctttggcg tgcttgttcg ggttggactc aagttgatag ttaccgaatt agcgcagtag
     3061 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctaaccggaa
     3121 ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa
     3181 ccgaaaggcg ggaacaggag agcgcacgag ggagccatca gggggaaacg cctggtatct
     3241 ttatagtcct gtcgggtttc gccaccactg atttgagcgt caaattttgt gatgcttgtc
     3301 aggggggcgg agcctatgga aaaacggccg ttgtgcggcc ttgtctctct tcgtgctgtc
     3361 tgtcctatgc cgttatgtgt gcgttgcgac cgctcgccgc agccgaacgg caggagcgta
     3421 gcgactgagt gagcgaggaa gcggaaaaga gactggtttt acactgagca ctgacgctc
//
