LOCUS       NZ_OW970448             5838 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 101 plasmid P4, complete sequence.
ACCESSION   NZ_OW970448
VERSION     NZ_OW970448.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082320
            Assembly: GCF_903993205.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993205.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:46:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,615
            CDSs (total)                      :: 5,497
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 54 of 132
            Pseudo Genes (incomplete)         :: 81 of 132
            Pseudo Genes (internal stop)      :: 19 of 132
            Pseudo Genes (multiple problems)  :: 17 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970448.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="101"
                     /isolation_source="Hospital Universitario Ramon y Cajal
                     (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            70..396
                     /locus_tag="LQV08_RS27835"
                     /old_locus_tag="AI2898V1_5290"
     CDS             70..396
                     /locus_tag="LQV08_RS27835"
                     /old_locus_tag="AI2898V1_5290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_069333984.1"
                     /translation="MEKRTKEIKIRLTESEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERVSLFVVLNSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            605..>1027
                     /locus_tag="LQV08_RS27840"
                     /pseudo=""
     CDS             605..>1027
                     /locus_tag="LQV08_RS27840"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1074..1559
                     /locus_tag="LQV08_RS27845"
                     /old_locus_tag="AI2898V1_5291"
     CDS             1074..1559
                     /locus_tag="LQV08_RS27845"
                     /old_locus_tag="AI2898V1_5291"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139103748.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRKSVSTAMAWIFICFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLANLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            1629..1853
                     /locus_tag="LQV08_RS27850"
                     /old_locus_tag="AI2898V1_5292"
     CDS             1629..1853
                     /locus_tag="LQV08_RS27850"
                     /old_locus_tag="AI2898V1_5292"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_227655332.1"
                     /translation="MEKQLLSALESLQTGYEQQQQAWQDSYSSLQHMFEATSQALAHSD
                     RVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2009..2341
                     /locus_tag="LQV08_RS27855"
                     /old_locus_tag="AI2898V1_5293"
     CDS             2009..2341
                     /locus_tag="LQV08_RS27855"
                     /old_locus_tag="AI2898V1_5293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000017531.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSVRVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2343..2627
                     /gene="nadS"
                     /locus_tag="LQV08_RS27860"
                     /old_locus_tag="AI2898V1_5294"
     CDS             2343..2627
                     /gene="nadS"
                     /locus_tag="LQV08_RS27860"
                     /old_locus_tag="AI2898V1_5294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            2819..3748
                     /locus_tag="LQV08_RS27865"
                     /old_locus_tag="AI2898V1_5295"
     CDS             2819..3748
                     /locus_tag="LQV08_RS27865"
                     /old_locus_tag="AI2898V1_5295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_069333986.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSVRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLSAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASAYNLQFAAPLD
                     ENEVSGVSKSIAKWTFSKFTQSSFISYVEKTHTSDIQSRRGQKGGKVSSGGGRSITSNS
                     ERQLKPWEDMSISRATFYRRRKNGNSII"
                     /name="replication initiation protein"
     gene            3729..5630
                     /locus_tag="LQV08_RS27870"
                     /old_locus_tag="AI2898V1_5296"
     CDS             3729..5630
                     /locus_tag="LQV08_RS27870"
                     /old_locus_tag="AI2898V1_5296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000407093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_069333985.1"
                     /translation="MEIVSFKVSQYRSISGEISSDNFNGLTIVGQNNSGKTNLLKAMRV
                     FFEGKFNSDLYDYEKDKPHGVKQGQTSFAVRFTFSESEINDVSEDLSQDNDDDDDDDDD
                     DSVIDLGVLGEQEELGDSISLDDGGTKQQETKPTQSRGRKLSLRDRYLQLFNMIDDSRL
                     NSPQNEVTVHLTISNKNNYFYTVFKGYKRKPEVTSSMYTSVERRFIEKILSSFECVYIP
                     SSKSIDDIYHNLLVPYVRSEVASAMKTSVQVIHDKLLEVSKKITNEMTLSGIDDCSFMI
                     KAPPSLDELFGRFEVSIKDTIENVISSKGMGLQCLSLFSSFKSISERKLSLGKKTIWMV
                     EEPESFLHPSLGRSCNHIFNSLMDVAYVLITTHSLSFVSDEPSKIIELYKDEAGVTKDK
                     KHEKRFNAVASIRENLGVRFSDFFNISSSAVFVEGITDKQYIESILKLTYESDEFKNRW
                     PFLRSAKVDEFGGVSQLGGFLKGCYEFISKEVPVISIFDGDEAGVKERTRLQSYFGKKQ
                     VRFESNKDYISVRSGFAIEGLFPDDFISDAMETHPSWFIGGKSVDADDVIEPFKVQDNK
                     KTNLSNFFLEKCRVQPICDWISRWEKVFNVIDSALRDKSESITDKKRTEDTSDNQAA"
                     /name="AAA family ATPase"
     Typing          387..1945
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccccgaaac ccctggcatc cactgcgaaa aattctcacg gtggtgcgaa ttttttctca
       61 gcggagccta tggaaaagcg aacgaaagag atcaaaatcc ggctcaccga aagtgagcat
      121 cagcgactgc ttgatcgctg cgaccggact caccttgcgg agtggctgcg tcagctaggt
      181 ctgggtgaac acacctcacg caagcgtcgt gtgcctgacg ttgcacccga actgcttcga
      241 caggtcagcg ggattggcaa caacctgaac cagattgccc gccgcctgaa ccaggctgat
      301 tccctgacgc cctctgaacg tgtctcactg tttgtcgtgc tgaacagcct cgaccgtcag
      361 cttggcgacc tactggagca aaaccgtgat cgttaaaatt cattcgcggg gcgcgggttc
      421 tggcagcggg ccggtggatt acctgctggg aaaagaccgg cagcgtgaac aagccagcgt
      481 gctgcgtggt aatcctgaac atgtgcgcga gctgattgac ggctgcgact ttgcccgcgc
      541 ttacacctcc ggcgtactgt catttcagga gccggacatt gcagacgcgg agaaatcgcg
      601 actgatggac gaatgggagc acacgctgct gaccggcctt gactgcgacc agtacgcctg
      661 cctgtgggta gagcaccgcg acaaggggcg gcttgagctg aattttgtca tcccgaacat
      721 cgaattgcag agcggaaaac gcctgcaacc ctactttgac cgcgccgacc gtccccgcgt
      781 caacgcctgg cagaccctga ccaacgaccg gctggggctg cgcgacccga acgacccgac
      841 ctatcgccgc ccgctgacgc aggccagcga tcttccccgc gacaaacagc aggcggcaga
      901 aaaaatcacc gccggcctga tgaacctgat gcagcagggc gtgatccgca gccggcagga
      961 cgtggtgacg cagctggaaa gctacggcct gaccgtggcg cgggaaacga aaagcagcat
     1021 cagcattgcc gacccggacg gcggccgaaa tatccggctg aaaggaatga tatatgagcg
     1081 agattttaaa tttggcgaag ggcttcgagg agaaatcgaa gcagcaggcg caggataccg
     1141 agcagagcgt gaagcgcgag ttcgcgaggc tggagacgtc tatcagcgcg gaactgcaat
     1201 caagctcgca gagcatcagc aacgctatcc gcgagcagaa cggcaggctg acgggcatgc
     1261 tcaggaagtc agtctcaaca gcaatggcgt ggatattcat ctgcttcgcc ctgctgatcg
     1321 gcataatggg cgggataatc tggtttcagg gcacgatcat cacctcgcgc ctgagcgaga
     1381 tggacagcta cagccagcag ctggcgaacc tgaaagcgaa aagcggcgcg ggcgtgacga
     1441 tttacagcga cgacacgcgc cggaacacct acctggtggt gctgccgaag caggcgcacg
     1501 gcgtggaaac ctacacgtcg acgaccgaca acaccgtggt gaaatacacg gcgaaataac
     1561 ccctccagag cgccctgaaa cggcacagcg cgtttttaac actgaggacg accaaaacca
     1621 tgaccgagat ggaaaaacag cttctgagcg ccttagagag cttacagaca ggctacgagc
     1681 aacagcagca ggcgtggcag gacagctaca gcagtttgca gcacatgttc gaggctacct
     1741 cacaggcgct ggcgcacagc gacagggtgt gtcagcactt gagcagtcag gtggacagct
     1801 tgcgcgcgca ggtcgagagc ttgagcagga acgtcagccg cttgatgcgc tgatcaggca
     1861 gcatgaccgc gcctacgccg agaagctgcg tgagcagcag ccagagccgc agaaaaaacc
     1921 gacccgctac tatggtccct ctatgtgacg gcttttccgg aatcagaatt aagtgtacta
     1981 tgtacgccat tgaaaacagg agacagccgt gagtcacgcg attgagttta tcgagacccc
     2041 gatgtttacc cgtcagataa aacagatcgc cacggatgaa gaactcaggg agctgcaaaa
     2101 gacgctgatt gagtcgccgg acaaaggcga tctcattcag aaaaccggcg ggctgcgcaa
     2161 aatcaggatg gcgacgggca accagggaaa aagcggcagc gtgagggtga tttactttct
     2221 ggccaccgcc gaggtgatct atctggtgat ggcctacccg aaaagcacca aagacagcct
     2281 gacggacgcg gaaaaagccg cgctgaagac gctgacccaa caactgaaag acgaggtgtg
     2341 acatgagttt ttttgacgag ctgaaagcat cgctggaaga agccgttgaa atcaaaaacg
     2401 gcgtcagaga gcctgcccgc gtcacccgtt acgagctggc agacgtgaaa gccatcaggg
     2461 cacagctgaa cgtatctcag agcgagatgg cgaaagtgct gggaaccagc ctcgacacca
     2521 tcaagagctg ggaaaccgga cgccgtaacc cgaccggact ggcggcgaag gtactggcaa
     2581 ccatccaggc gaacccgaaa ttcttccagg aactggcggc gcactaagcc gttgtgacag
     2641 aaggattcga caggcgaggc cgttcgcgtg taatttaacg gcaacagaaa aaacaaagcc
     2701 ccggaaatca tgtcatatct tggcggatcg catgataacc agggccaggt cgaaaccttg
     2761 aaaggatatt agcacatgaa acgtgccaga caacagcccg ccataagcgc agggcgaatt
     2821 gaataacgca gtactcgaac ttttcaacga ccgcttaccg cacaagccgt acttttcgga
     2881 cgatctgcac tttggtgtac gcattgccgg taaggaacgc gccattctgg cgaaatacat
     2941 ccagttcaat cagcctcacg ccatgttctg gctgggattc gacgtcgatc gcgccggtgc
     3001 cgctattgac tggagcgacc gcaatgcccc cgcgccgaca ctgacgatca ccaaccccga
     3061 caacggacac gcgcacctgc tgtacgcgct gaaaacctct gtacgtaccg cgccggacgg
     3121 aaaaatgaag ccgctgcgct acgccgccgc cgtggagaac gcgctgcgta aaaaattaga
     3181 ggctgacgcg gggtattcgg gactaatctg caaaaatccg aaccacggcc actggaaaat
     3241 tgctgtatgg cagccggaac tctacacgct ggactggctg gccgactttc tcgacctgag
     3301 cgctgcgaat gacaaagaaa tcgttgccga ctacggctta ggccgtaact gcaccctgtt
     3361 cgataaaacc cgcaaatggg cataccgcgc tatccgtcag ggctggcctg agtatgacca
     3421 gtggctgcaa gcctgttatg aacgcgccag cgcctataat ctgcaatttg cagcgcctct
     3481 ggatgaaaat gaggttagtg gagtttcaaa aagtatagct aaatggactt tttcaaaatt
     3541 cactcaaagc tcttttattt cttatgttga aaaaactcat acgagtgaca ttcaatcgcg
     3601 taggggccag aaaggcggga aagtgagttc cggtggagga aggtcaatta cttcaaatag
     3661 cgaaagacag ttaaagcctt gggaagacat gtcgataagt cgcgctacat tctatcgtag
     3721 gaggaaaaat ggaaatagta tcatttaaag tatctcagta taggtctatt tcaggagaga
     3781 tatcctctga taactttaat ggattaacca ttgttgggca gaacaatagt ggtaaaacaa
     3841 atctattaaa agccatgagg gttttttttg aaggtaaatt taattctgac ttatatgact
     3901 atgaaaaaga taaaccacat ggtgttaagc aaggacagac cagttttgct gtaagattta
     3961 ctttttctga aagtgagatt aatgatgtct ctgaggattt gagccaagat aatgatgatg
     4021 atgatgatga tgatgatgat gactcggtta ttgatcttgg tgttttagga gagcaagaag
     4081 aacttggaga ttctatttca cttgatgatg gaggcactaa acaacaagaa acaaagccaa
     4141 ctcaatcaag aggcaggaag ctaagtttga gagataggta tttgcagcta tttaacatga
     4201 ttgatgactc taggttaaat tcacctcaaa atgaagtgac cgtgcactta acaatatcta
     4261 ataaaaataa ttatttctat actgttttca aaggttacaa gaggaagccc gaagtcactt
     4321 cctccatgta tacatctgtt gaaagaaggt tcatagaaaa aatactttct agttttgaat
     4381 gtgtatatat accatcctca aaaagcatcg acgatatata tcataatcta ttagtgccat
     4441 atgttaggag tgaagtcgct tcagccatga aaacatcggt tcaagtgatt catgataagc
     4501 tgctggaagt gtcgaaaaag ataacaaatg aaatgactct aagtggtata gatgactgta
     4561 gttttatgat taaagctcca ccatctctgg atgagttatt tggtcgcttt gaggtttcta
     4621 taaaagatac tattgagaat gtgatctcga gtaaaggtat ggggctacaa tgcttatctt
     4681 tattttcttc atttaaatcc atttcagaga gaaaactttc gctaggtaag aaaacgattt
     4741 ggatggttga ggagcctgag tctttcttac atccatcatt agggcggtca tgtaatcaca
     4801 tattcaattc attgatggat gttgcttatg ttcttattac aactcattcc ttatcatttg
     4861 tcagtgatga gccaagcaag ataatcgagc tatataaaga tgaagccggt gttacaaaag
     4921 ataaaaagca tgagaaaaga tttaatgcag ttgcaagcat aagagaaaat cttggggtca
     4981 gattttctga tttctttaac atatcaagca gtgcagtttt tgttgaaggc atcaccgaca
     5041 aacaatatat agagtcaata ttgaagttaa cgtatgaaag tgatgagttt aagaataggt
     5101 ggcctttcct gagaagtgct aaagttgatg agtttggcgg tgtcagtcaa ctgggagggt
     5161 tcttaaaggg ctgctacgag tttatttcta aagaagtccc tgtgatatcc atttttgacg
     5221 gtgatgaagc tggggttaaa gaaaggacta gattgcaaag ttactttggt aaaaaacaag
     5281 ttagatttga gtcaaataaa gattatatct ctgtaagaag tggttttgca attgaaggcc
     5341 tcttccctga cgatttcatt tctgacgcaa tggaaaccca tccgtcgtgg tttatcggag
     5401 gaaaatctgt tgatgccgat gatgtaattg aaccattcaa ggtgcaagat aataaaaaaa
     5461 ctaacttatc aaatttcttc ttggaaaaat gtcgcgttca acctatatgc gattggattt
     5521 ctcgatggga aaaggtgttt aacgtcatag actcagcctt gagagataaa agcgaatcca
     5581 tcacagacaa aaaacgaacc gaagatacct cagataatca agctgcataa tcgatagtga
     5641 gcgccgaggc tgctcatgca cctgattaat caagagtgag accataacaa gcctatatca
     5701 gataacagcc ctctgctaag agggcttttt tttttgcagc cagcactgaa tctgacatac
     5761 tgcactttac cctaagccag tacacaccgc gtcccctcta acgaggtgcc accgtgactg
     5821 gttcaggggg ctcgccgc
//
