LOCUS       NZ_OW970427             4715 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 147 plasmid P5, complete sequence.
ACCESSION   NZ_OW970427
VERSION     NZ_OW970427.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082330
            Assembly: GCF_903993095.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993095.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:01:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,492
            CDSs (total)                      :: 5,371
            Genes (coding)                    :: 5,257
            CDSs (with protein)               :: 5,257
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 114
            CDSs (without protein)            :: 114
            Pseudo Genes (ambiguous residues) :: 0 of 114
            Pseudo Genes (frameshifted)       :: 46 of 114
            Pseudo Genes (incomplete)         :: 66 of 114
            Pseudo Genes (internal stop)      :: 15 of 114
            Pseudo Genes (multiple problems)  :: 11 of 114
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970427.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="147"
                     /isolation_source="Hospital Univeristario de Bellvitge
                     (Barcelona)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            complement(639..833)
                     /locus_tag="LQV34_RS27310"
                     /old_locus_tag="AI2770V1_5188"
     CDS             complement(639..833)
                     /locus_tag="LQV34_RS27310"
                     /old_locus_tag="AI2770V1_5188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1909..2865
                     /locus_tag="LQV34_RS27315"
                     /old_locus_tag="AI2770V1_5189"
     CDS             1909..2865
                     /locus_tag="LQV34_RS27315"
                     /old_locus_tag="AI2770V1_5189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000280763.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            2849..3715
                     /locus_tag="LQV34_RS27320"
                     /old_locus_tag="AI2770V1_5190"
     CDS             2849..3715
                     /locus_tag="LQV34_RS27320"
                     /old_locus_tag="AI2770V1_5190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            3697..4365
                     /locus_tag="LQV34_RS27325"
                     /old_locus_tag="AI2770V1_5191"
     CDS             3697..4365
                     /locus_tag="LQV34_RS27325"
                     /old_locus_tag="AI2770V1_5191"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_223195384.1"
                     /translation="MGYIQMIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCS
                     EWNMKILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYN
                     KSGVLLTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFD
                     LNDENKEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS
                     "
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(4587..4700)
                     /locus_tag="LQV34_RS27500"
     CDS             complement(4587..4700)
                     /locus_tag="LQV34_RS27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427893.1"
                     /translation="MHEPPVQPDCCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="hypothetical protein"
     Typing          482..566
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          870..968
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1093..1348
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          complement(2..471)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.08"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4592..4715)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.2"
                     /coverage="100.0"
                     /evalue="1.51e-47"
ORIGIN
        1 gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa gcgccacgct
       61 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      121 cacgagggag ccaccagggg gaaacgtctg gtatctttaa gtcctgtcgg gtttcgccac
      181 ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      241 gcctgcggcg cggccttctt cctgttcttg gtcttttgct cacatgttct ttccggcctt
      301 atcccctgat tctgtggata accgtgctgc cgtttttgtg taagtcagta ccgctcgccg
      361 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgc
      421 gcattttctc cttacgcatc tgtgcggaat ttcacacccg gcatggggca cttttcatac
      481 aattcgcact gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
      541 gtcagggctt cgccccgaca cccgctgacg cgccctggcg ggcttgtctg ctgccgaaaa
      601 cgctcgcgga caagccgtga gcgcctccgt cagcttcgtt atgttcctgt ttcactttcg
      661 gtctcaaagc gaacctggat gctgttctgg agttcttccg cgagttcgtg cagtcgttcg
      721 cacatggccg cctgttcgtc ggcatccaga gcatccagtt tttcaagcag tgtcaggctc
      781 tggcttttga taaatcgcgc catattcagc gcagtttgtt gctgtttgtt caccttccgt
      841 tgttctccgt cagtaccggg tgtcgggtgg accctgacca ggtggtaatt gtaagacgtt
      901 gcgggcgacg gtattacaat tgcacatcct gtcctgtttt tttaggacgt atcatggcac
      961 agcatatcaa ctgataaata atccctgtgc agtgcagccg ctgttgcata aaatttcgct
     1021 ctgcgtcgag tgtttcttaa tcaataattc cgctgatact ccggaaaaac gcgagtctgt
     1081 tctcatgcct gaaatgccca caccccacgc aaaaacaatt ttttgctgat ttttctttat
     1141 aaatagagag ttatgacaaa ttagttcttc ttgctctctt tgtgatattt aaaaaagcgg
     1201 tgtcggcgcg gtgttgtagc tgcgccaaca ccgcttttta ggggtggtac tgactatttt
     1261 cataaaaaac atcattttat attagggggg ctgctagcgg cgcggtgtgt ttttttacag
     1321 gacgcccctg ggggcgctgc taggggtgtc tgttcagata tgtagcaatg agtcagggat
     1381 tcacgaactg aaaatgtccg aaaatgtgtc tcatgggcac tgtatgcgcc caaaccgatc
     1441 gtcattcact ttcatgcata gctatgcagt gagctgaaag cgatcctgac gcatttttcc
     1501 ggtttacccc ggggagaaca tctctttttg cggtgttcgc ggcagaatcg cgttcagcgc
     1561 gttttagcgg tgcgcgtaat gcgacgttat ggtaaatgac gctgaaaaat gatcttttac
     1621 gctcatcgtt tatgagcaac agatgtccta atcatggatt tgaactataa ccggcgctga
     1681 attgatcgca tgaggtgtta tgaaaacggt gatataagta aactttttct tgacttccta
     1741 cttattatcc tataaaataa atccttagga tttattttat aggatatcaa aaatataaaa
     1801 atggcaaatg cttgtttgtt attatttttt tcttgacaat caatctctaa tagaatatac
     1861 gtccttagga cgtatattct attagagact aaaccgaaaa ggttgaatat gtatcttatc
     1921 gaagaagcgc taacgtcttg gttgttgaat gaatataaaa aaccagtcat aaccaaagaa
     1981 gaaatctctt tgcagattta ccttttttat ttaaaaaaag aatataatgg tgagaggata
     2041 tatcaaatta gaaagaacaa agcaggacct aatgaatttg aaaaaaatat attaaaatta
     2101 ttaaagtcag gggttttatc aagagttgaa agtaacgata agcatcatgc ttacccccat
     2161 ttaattggcg gtttctatga ggtctttttt gttaaaggta acgatggttc tccggaaaat
     2221 gatatagtgt gctctacgta tccatatgga tacttatcct atttatccgc tatggattgg
     2281 cataacatca cagataaaat acccaagata atccacttta cgacatgtcg ttccgaacag
     2341 tggaagaata agttctatca tgattttcaa aaggataatc ctgatataga cgaagccaaa
     2401 caattcatgc ctagatttcc aagaaattct gaggtcaatg gaataaaaat tatcgttaca
     2461 caagaaacaa attatgttga gccaatgaag gctcgtaatt cccctgtcag ggtatcatct
     2521 atcggtaaga cctttatgga tatgaccagg cggcctgatt actgtggtgg tgaggagcat
     2581 gtattggaat catttattga gcacggtaaa aaatatgcac ctctgattat aaaggaagtc
     2641 gataagcttg gccgtcatat tgacaaggta agggttggat tcattttgga tagaattatt
     2701 ggggttaata ataaaaaaat aagcgaatgg aaacaagagg cgggtaaata cagaggtagt
     2761 tcgaaagtgc tatctccaaa gagtccattc tcaccttact ttgatgaaga atggtttatt
     2821 tctattaatg ttgatatggt taagtcttat ggaaaaagaa attaataatt ggatcgaaga
     2881 gtctggtggg aagtacaggt tgatgcgcca ggctgtacat ataatactta aatctatttc
     2941 tatagaggag agtttgaatg aaatgatggt tatgaaggga gggactcttc ttggtgttaa
     3001 gcatggtagt gatagataca caaaggatat tgatttttct accgaaaaaa aactttctga
     3061 aattgacttg ggggattttt tcaataggtt taatgactca atggatgttg ctgaggctga
     3121 attgggatat ggtattaaat gtaaagttca atctcataaa attcaaccta atgcacaagg
     3181 aacatttcca actgtacaga taaatatagc cttttgcgat cgttctaatg cgtctgcaat
     3241 gaaaaggctt gaatctaatc aatcaccttc agtgataaat atcgatttta gctttaacga
     3301 aaaaacgtat gattttgact ttttatcatt agatggtgat gatgacaatg atagcgtaaa
     3361 gacctacagc ttaactgatt tgatggctga aaaatatcga tccgttttgc aacaaaaagt
     3421 tagagagaga aatcgagagc aagatatata tgatatcaat tatcttttag gtaaatatga
     3481 gttttctcgg caagataagt ataaaatact ggagtctctt ttaaaaaaga gtgaaggtaa
     3541 acaattggat aatctcttaa acgttaaagg tataagggat gtagaaatta tcgagcgttc
     3601 cagtcagcga tatcaggatt tatcatccac cgttaaatca ttacctcttt ttgaagatgc
     3661 ttatgaaaaa gttgcctgtt tctatgaaag tcttccgtgg gatatattca aatgatagcg
     3721 ttcagtttta gtgatattgg taacagatgt gaaaacttag acttattatc tacgataaat
     3781 tgtcaaaatg atgtgtttgg aatggttatt atggatgggt attcctgctc tgagtggaat
     3841 atgaaaattc tctctggtag gttagataag tgcgtgtttt ctggtgatct gaataaagat
     3901 attattgaac catgcaagtc agttcctgtt aaggctgcag tatgtttcct ttttaaaaat
     3961 aatacgcacc tccatatata taacgcagga gattgccgtg tatataacaa aagtggagtc
     4021 cttctaacag aggataactc ggttgcctgg aaaagattaa aggaaaatgg aatcgataat
     4081 aaaaaaatac ctcacctcgt ttgttattgc ccagacagaa attatctaac atcatttgtt
     4141 gatttctgct ctgatgagat aattttcaat cattcagttt ttgatttaaa tgatgaaaac
     4201 aaagaggtta ttttatgtac tgatgggttt tgggagcatt ttaatgatgg tactgttatt
     4261 gacctcatga aagataaccc agaatctaac ttggctaaat ttactaaaaa tatcccgggt
     4321 aacgcagaga atcatacggt ttgctacgtt agattccctt cttgatttcg tgttttttga
     4381 agaagctatt gagatcttcg tgtcatatag ataatctctt ttctgtaaaa gaaaaaaacc
     4441 gcctttgcgg ggcggttttc tcttaggttc aaagagtttg cggctcgaag aaccaaggta
     4501 actggcttgg aggagcgcag tcaccaaaac ctgttctttc agtttagcct taactggcgc
     4561 atgacttcaa gactaactcc tctaaatcag ttaccagtgg ctgctgccag tggcgttaag
     4621 gcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcagtcgggc
     4681 tgaacggggg gttcgtgcac acagcccagc ttgga
//
