LOCUS       NZ_OW970378             4714 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 101 plasmid P4, complete sequence.
ACCESSION   NZ_OW970378
VERSION     NZ_OW970378.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082336
            Assembly: GCF_903993065.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993065.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:54:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,629
            CDSs (total)                      :: 5,509
            Genes (coding)                    :: 5,370
            CDSs (with protein)               :: 5,370
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 54 of 139
            Pseudo Genes (incomplete)         :: 83 of 139
            Pseudo Genes (internal stop)      :: 24 of 139
            Pseudo Genes (multiple problems)  :: 17 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970378.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="101"
                     /isolation_source="Hospital Universitario Valencia Tres
                     cruces (Valencia)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            complement(join(4677..4714,1..76))
                     /locus_tag="LQV21_RS28210"
     CDS             complement(join(4677..4714,1..76))
                     /locus_tag="LQV21_RS28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071785923.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            492..683
                     /locus_tag="LQV21_RS27975"
                     /old_locus_tag="AI3007V1_5316"
     CDS             492..683
                     /locus_tag="LQV21_RS27975"
                     /old_locus_tag="AI3007V1_5316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            683..1084
                     /locus_tag="LQV21_RS27980"
                     /old_locus_tag="AI3007V1_5317"
     CDS             683..1084
                     /locus_tag="LQV21_RS27980"
                     /old_locus_tag="AI3007V1_5317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2126..2395
                     /locus_tag="LQV21_RS27985"
     CDS             2126..2395
                     /locus_tag="LQV21_RS27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024167138.1"
                     /translation="MSITMSQKSAREGLGNPELFQGGVYVTKNGLAELFVQTAEEREAE
                     LRERERERQSNALLKLVMAAKREIKNGRGMSPEDAMRRLREART"
                     /name="hypothetical protein"
     gene            2410..2754
                     /locus_tag="LQV21_RS27990"
     CDS             2410..2754
                     /locus_tag="LQV21_RS27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032431017.1"
                     /translation="MANSFNVTIAEAALWSLQDAESFKSNYTDSVQAALWADDLLASSI
                     KAISQDPDRYRYNAMLADKGISIRERLDPDNQYRCLYDFDEQNGVIEILLFVSTKQDLE
                     KMLYRYLVVK"
                     /name="hypothetical protein"
     gene            complement(2842..3273)
                     /locus_tag="LQV21_RS27995"
     CDS             complement(2842..3273)
                     /locus_tag="LQV21_RS27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086073960.1"
                     /translation="MDWYDVKWFFGDVCDSVSEVAGNIYDTAADGVAAVTDFIEENPKT
                     SIAIGALVGIATAGIAAPAIAATIGATGALGTTAGGTVIANLSGAALSSSSLAALGGGS
                     IAAGGAGMAGGTCVVAAGGGVVAGSVSAGGVAATKAIKS"
                     /name="hypothetical protein"
     gene            complement(3598..4269)
                     /locus_tag="LQV21_RS28000"
                     /old_locus_tag="AI3007V1_5318"
     CDS             complement(3598..4269)
                     /locus_tag="LQV21_RS28000"
                     /old_locus_tag="AI3007V1_5318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_179186626.1"
                     /translation="MLIVILSLYYTITEPFKSELIKNLSYSLIATFIFDTGLNFSKENI
                     TKSIVSRKWHNSLYFAFERLRAIHEIFTAQNKLEITKELASDMASSILMENGAVISKKH
                     IKTMWNPSSGEPLTYQDIVIKEGDNLYFASMMFIRDDYEFLHSIYFDENIFLSFPQLLK
                     PAQKCYRSLAMIQHNINSKQKFNVTKKSLERDLINYLELRSTLLMKMEDVLGHYEQRAP
                     Q"
                     /name="hypothetical protein"
     Typing          complement(2..371)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.418"
                     /coverage="90.0"
                     /evalue="1.66e-166"
ORIGIN
        1 gtctttctgg gttggactca agacgatagt taccggataa ggcgcagcag tcggactgaa
       61 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
      121 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
      181 ggaacaggag agcgcacgag ggagccatca gggggaaacg cctgatatct ttatagtcct
      241 gtcgggtttc gccaccactg atttgagcgt cagtttctgt gatgcttgtc aggggggcgg
      301 agcctatgga aaaacggccg ctgcgcggcc tttccttctt tccaccatgc gattgctcgg
      361 ttttctcgag cgtttctacg catgctcctg ccgctcgccg cagccgaacg gcaggagcgt
      421 agcgactgag tgagcgagga agcggaaaag agaacatccc tacactgagc actgacgctc
      481 tgaggcctct tatgacgaaa caacagaaaa cagcgctgaa tatggctaaa ttcatccaga
      541 accagtctct gctcctgctg gagaagctta acgagcttga tctcgatgtc gaagccgacc
      601 tttgtgagaa actccacgat gatgcggagc atcttttccg tacactgtcc tcgagactgg
      661 atgcattaca ggatggtaac tgatggccag agtgtctatt agtgaggctg cccgactgac
      721 gggtaagagt agaacaacac tgcacaggtt aattaaagca ggtgacctgt ccacctgtag
      781 cggtgaacgc aatgcaaaaa tggtagatac ttcagagctt ttacgtgtct ttggtccctt
      841 tgaacaaccg aagggtgaac accattctgg acaggtctct gaacagcgtg atacagcctc
      901 tgctgaccag agtgaacagg tgattcgtca tctgaaacag gagattgaac acctgaacac
      961 cttacttttg tccaaggatt cgcacattga cagcctgaaa caggccatgc tccttcttga
     1021 aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct ggaaaaaatc
     1081 ctgaccccgt catgcttcag ttttcctctc cggccatccc ggggagttcc tgaaccttcc
     1141 caaaaatcgt cagatctctg aacgctcctc accactaaaa atacccacac cccacgcaaa
     1201 attaaaattt tgaagataac tcattaatat cagttagttg tgggttatta aattacgcag
     1261 tattactttc tgtaaggtga atccaagtcg tcggcgattc gtgtacgact tgccgacgac
     1321 tcgtcataga gtcatcgacg actcacttaa gactatcagt aacttatgac gacttgactc
     1381 tagagtcgaa tcgtcatttt ggtgtttttt tgaccttgac gactctagag tcaagtcacg
     1441 agtcgaatcg ccggtgaatt gtctggtgag tcgtccggcg acttgccata acgtgattgc
     1501 cggccgtcga cgaacccgat tcctgatcgt gctgaaaacc ggttttccgg ctgcgccggc
     1561 aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga tggaaagaac
     1621 gccggaaaca ggacttatcc tggctgaatg gcgtgaattt tcgcgctatg cacttgcgtg
     1681 catactcatg catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt
     1741 tgttgcctgt tttaccggtt tcccgtcaga aacgccctga ggccgttctg gcggtgcgta
     1801 caatcatggc tttatgttaa ataacagacg aaaaaatctc gatagtagaa gaatggggat
     1861 tgttcctgag ctggggatgc cttactgatg cgcgtaattt tcgtttaata ggaggttggt
     1921 cgaagctcca gaatcttata tttgaatggt ggtgtatata tttatgataa atttgtactc
     1981 ccctgtgagt tttgatacaa tataactgcc attcacccta agtggcagca agatgtgagg
     2041 tctttttgac ctctttttca ttcaggatct agtcgcttat gcttaatctt gcgtgtaagt
     2101 ggctgtttta attaaaggta taagtatgtc tataactatg tctcagaagt cagcccgtga
     2161 aggtcttggc aaccctgaac tgttccaagg aggcgtttat gttacgaaaa atggtttagc
     2221 agagctgttc gtacagaccg cagaagagag agaggccgag ctgcgtgaga gagaaagaga
     2281 acggcaatca aatgctcttt tgaagctagt catggctgca aagagagaga taaaaaatgg
     2341 tcgtggaatg tcccccgaag atgctatgcg gaggttacgt gaagcgcgaa cttaatataa
     2401 ggagccaact tggcaaattc attcaatgta acgatcgcgg aagcggcact atggtctctg
     2461 caggatgcag aatcctttaa atcaaactac acggatagtg ttcaagctgc gttatgggct
     2521 gatgatcttt tggcttcatc gataaaggcc atttctcaag atcctgatcg ttatcgctac
     2581 aatgctatgt tggctgataa aggaatatcg attcgtgagc gtttggaccc cgataaccag
     2641 tatcgttgtt tatatgattt tgatgaacaa aatggtgtca ttgagatcct gctttttgtt
     2701 agcactaagc aagatcttga gaaaatgctt tacagatatc ttgttgttaa atagatgtta
     2761 aatgcaaaag cccgctttta tagtgggctt tttatatggt agaaacttta agatgtcgac
     2821 gttatggtaa atggaagctt gttagctttt tatcgctttt gttgctgcca ctcctccagc
     2881 agaaacgctt ccagcgacca ctcctcctcc cgcggctact acacatgttc cgccagccat
     2941 accggctcct ccagctgcga tggaacctcc acccaacgct gctaaagacg agctagataa
     3001 tgctgctcca cttaagtttg ctattacggt acctccagct gtagttccta gcgctccggt
     3061 tgctccaatt gttgccgcta ttgccggagc tgctatgcct gctgtcgcaa tacctacaag
     3121 ggctcctatc gcaatggatg tttttggatt ttcttcaatg aaatcagtta cagccgctac
     3181 cccatcagct gcagtatcgt aaatattgcc tgccacctct gaaacagagt cacagacgtc
     3241 tccgaaaaac catttaacgt cgtaccagtc catatgctac cctcttaacc atatgattta
     3301 ttaagtaatt aatctttttt gattgcacgg aagatataat acacatatta aaagcaatgt
     3361 tttttaaagg caataaaatg atcgcaggcc catgcataat tatggtgagt ggaactggtt
     3421 atgttggtaa attcttcgat ggttcgatag attggattga aagttgctac aaatagcttt
     3481 cagggggggg gggggggaac gccaaacgtt ttttatttat gcacaatcat ttctttatgt
     3541 tcaatcttac ttatctcatt gcctttttta aatttaaaga tgaaagtgac attgatatta
     3601 ttgtggtgct ctttgttcat agtggccaag aacatcttcc attttcatta ataatgtaga
     3661 tctcagttct aaataattaa ttagatctct ttctaatgat ttttttgtga cgttaaattt
     3721 ttgtttgctg tttatattat gctgtatcat tgctagtgat cggtaacatt tttgggctgg
     3781 ttttagtagt tgagggaagg agagaaatat attttcatca aaataaatac tgtgtaggaa
     3841 ttcataatca tctctgatga acatcataga cgcaaagtat aagttatccc cttcttttat
     3901 tactatgtct tgatacgtaa gtggttctcc tgaagaaggg ttccacattg tttttatgtg
     3961 ttttttagat ataacagctc cattttccat tagtatggat gacgccatgt cgctggctag
     4021 ttcttttgtg atctctagtt tgttttgtgc tgtgaatatt tcatgtattg ctcttaatct
     4081 ttcaaatgca aagtataaag aattgtgcca tttcctggat actatacttt ttgttatatt
     4141 ttctttgctg aagtttaagc cggtgtcaaa aataaaagtt gcgattaatg aataggataa
     4201 gtttttaatt aattctgatt taaatggttc tgtaattgta taatagagag ataatataac
     4261 aattaacatt agtgattgtc gtattgcacc agatgaatat atgtataaac gatatctatt
     4321 taatggtgct tttatttttt gagggattag agatattgat ttatattgtc tcctcggggg
     4381 ggtgaaacaa aaaatacatg tgtatgtgaa taatataatg aataatataa agttaaccaa
     4441 tcttatcact cctcgatatc ggtgatgaat gattttcttg agatcttcag caagtcatcg
     4501 taatctcttc cctgctaata agaaaaaacc gccttgcagg gcggtttttt cgaaggttct
     4561 aagagttggc gctcgttgaa cctaggtaac tggctagtta gaggagcaca gtcaccaaaa
     4621 ctgttcgttt agtttagcct taactggcgc ataacttcaa gactaactcc tctaaatcag
     4681 ttaccagtgg ctgctgccag tggcgataag tcgt
//
