LOCUS       NZ_OW970377             5838 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 101 plasmid P3, complete sequence.
ACCESSION   NZ_OW970377
VERSION     NZ_OW970377.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082336
            Assembly: GCF_903993065.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993065.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:54:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,629
            CDSs (total)                      :: 5,509
            Genes (coding)                    :: 5,370
            CDSs (with protein)               :: 5,370
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 54 of 139
            Pseudo Genes (incomplete)         :: 83 of 139
            Pseudo Genes (internal stop)      :: 24 of 139
            Pseudo Genes (multiple problems)  :: 17 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970377.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="101"
                     /isolation_source="Hospital Universitario Valencia Tres
                     cruces (Valencia)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P3"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            complement(join(5652..5838,1..1715))
                     /locus_tag="LQV21_RS27935"
     CDS             complement(join(5652..5838,1..1715))
                     /locus_tag="LQV21_RS27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000407093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_069333985.1"
                     /translation="MEIVSFKVSQYRSISGEISSDNFNGLTIVGQNNSGKTNLLKAMRV
                     FFEGKFNSDLYDYEKDKPHGVKQGQTSFAVRFTFSESEINDVSEDLSQDNDDDDDDDDD
                     DSVIDLGVLGEQEELGDSISLDDGGTKQQETKPTQSRGRKLSLRDRYLQLFNMIDDSRL
                     NSPQNEVTVHLTISNKNNYFYTVFKGYKRKPEVTSSMYTSVERRFIEKILSSFECVYIP
                     SSKSIDDIYHNLLVPYVRSEVASAMKTSVQVIHDKLLEVSKKITNEMTLSGIDDCSFMI
                     KAPPSLDELFGRFEVSIKDTIENVISSKGMGLQCLSLFSSFKSISERKLSLGKKTIWMV
                     EEPESFLHPSLGRSCNHIFNSLMDVAYVLITTHSLSFVSDEPSKIIELYKDEAGVTKDK
                     KHEKRFNAVASIRENLGVRFSDFFNISSSAVFVEGITDKQYIESILKLTYESDEFKNRW
                     PFLRSAKVDEFGGVSQLGGFLKGCYEFISKEVPVISIFDGDEAGVKERTRLQSYFGKKQ
                     VRFESNKDYISVRSGFAIEGLFPDDFISDAMETHPSWFIGGKSVDADDVIEPFKVQDNK
                     KTNLSNFFLEKCRVQPICDWISRWEKVFNVIDSALRDKSESITDKKRTEDTSDNQAA"
                     /name="AAA family ATPase"
     gene            complement(1696..2625)
                     /locus_tag="LQV21_RS27940"
                     /old_locus_tag="AI3007V1_5310"
     CDS             complement(1696..2625)
                     /locus_tag="LQV21_RS27940"
                     /old_locus_tag="AI3007V1_5310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_069333986.1"
                     /translation="MNNAVLELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPDNGHAHLLYALKTSVRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHGHWKIAVWQPELYTLDWLADFLDLSAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYDQWLQACYERASAYNLQFAAPLD
                     ENEVSGVSKSIAKWTFSKFTQSSFISYVEKTHTSDIQSRRGQKGGKVSSGGGRSITSNS
                     ERQLKPWEDMSISRATFYRRRKNGNSII"
                     /name="replication initiation protein"
     gene            complement(2817..3101)
                     /gene="nadS"
                     /locus_tag="LQV21_RS27945"
                     /old_locus_tag="AI3007V1_5311"
     CDS             complement(2817..3101)
                     /gene="nadS"
                     /locus_tag="LQV21_RS27945"
                     /old_locus_tag="AI3007V1_5311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_001697687.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="nadS"
     gene            complement(3103..3435)
                     /locus_tag="LQV21_RS27950"
                     /old_locus_tag="AI3007V1_5312"
     CDS             complement(3103..3435)
                     /locus_tag="LQV21_RS27950"
                     /old_locus_tag="AI3007V1_5312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000017531.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSVRVIYFLATAEVIYLVMAYPKSTKDSLTDAEKAALKTLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3591..3824)
                     /locus_tag="LQV21_RS27955"
                     /old_locus_tag="AI3007V1_5313"
     CDS             complement(3591..3824)
                     /locus_tag="LQV21_RS27955"
                     /old_locus_tag="AI3007V1_5313"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_065801905.1"
                     /translation="MTEMEKQLLSALESLQTGYEQQQQAWQDSYSSLQHMFEATSQALA
                     HSDRVCQHLSSQVDSLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3885..4370)
                     /locus_tag="LQV21_RS27960"
                     /old_locus_tag="AI3007V1_5314"
     CDS             complement(3885..4370)
                     /locus_tag="LQV21_RS27960"
                     /old_locus_tag="AI3007V1_5314"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_139103748.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTEQSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMLRKSVSTAMAWIFICFALLIGIMGGIIWFQGTIITSRLSEMD
                     SYSQQLANLKAKSGAGVTIYSDDTRRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<4417..4839)
                     /locus_tag="LQV21_RS27965"
                     /pseudo=""
     CDS             complement(<4417..4839)
                     /locus_tag="LQV21_RS27965"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5048..5374)
                     /locus_tag="LQV21_RS27970"
                     /old_locus_tag="AI3007V1_5315"
     CDS             complement(5048..5374)
                     /locus_tag="LQV21_RS27970"
                     /old_locus_tag="AI3007V1_5315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_069333984.1"
                     /translation="MEKRTKEIKIRLTESEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQADSLTPSERVSLFVVLNSLDRQLGDLLEQ
                     NRDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3500..5058)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accttgaatg gttcaattac atcatcggca tcaacagatt ttcctccgat aaaccacgac
       61 ggatgggttt ccattgcgtc agaaatgaaa tcgtcaggga agaggccttc aattgcaaaa
      121 ccacttctta cagagatata atctttattt gactcaaatc taacttgttt tttaccaaag
      181 taactttgca atctagtcct ttctttaacc ccagcttcat caccgtcaaa aatggatatc
      241 acagggactt ctttagaaat aaactcgtag caacccttta agaaccctcc cagttgactg
      301 acaccgccaa actcatcaac tttagcactt ctcaggaaag gccacctatt cttaaactca
      361 tcactttcat acgttaactt caatattgac tctatatatt gtttgtcggt gatgccttca
      421 acaaaaactg cactgcttga tatgttaaag aaatcagaaa atctgacccc aagattttct
      481 cttatgcttg caactgcatt aaatcttttc tcatgctttt tatcttttgt aacaccggct
      541 tcatctttat atagctcgat tatcttgctt ggctcatcac tgacaaatga taaggaatga
      601 gttgtaataa gaacataagc aacatccatc aatgaattga atatgtgatt acatgaccgc
      661 cctaatgatg gatgtaagaa agactcaggc tcctcaacca tccaaatcgt tttcttacct
      721 agcgaaagtt ttctctctga aatggattta aatgaagaaa ataaagataa gcattgtagc
      781 cccatacctt tactcgagat cacattctca atagtatctt ttatagaaac ctcaaagcga
      841 ccaaataact catccagaga tggtggagct ttaatcataa aactacagtc atctatacca
      901 cttagagtca tttcatttgt tatctttttc gacacttcca gcagcttatc atgaatcact
      961 tgaaccgatg ttttcatggc tgaagcgact tcactcctaa catatggcac taatagatta
     1021 tgatatatat cgtcgatgct ttttgaggat ggtatatata cacattcaaa actagaaagt
     1081 attttttcta tgaaccttct ttcaacagat gtatacatgg aggaagtgac ttcgggcttc
     1141 ctcttgtaac ctttgaaaac agtatagaaa taattatttt tattagatat tgttaagtgc
     1201 acggtcactt cattttgagg tgaatttaac ctagagtcat caatcatgtt aaatagctgc
     1261 aaatacctat ctctcaaact tagcttcctg cctcttgatt gagttggctt tgtttcttgt
     1321 tgtttagtgc ctccatcatc aagtgaaata gaatctccaa gttcttcttg ctctcctaaa
     1381 acaccaagat caataaccga gtcatcatca tcatcatcat catcatcatc attatcttgg
     1441 ctcaaatcct cagagacatc attaatctca ctttcagaaa aagtaaatct tacagcaaaa
     1501 ctggtctgtc cttgcttaac accatgtggt ttatcttttt catagtcata taagtcagaa
     1561 ttaaatttac cttcaaaaaa aaccctcatg gcttttaata gatttgtttt accactattg
     1621 ttctgcccaa caatggttaa tccattaaag ttatcagagg atatctctcc tgaaatagac
     1681 ctatactgag atactttaaa tgatactatt tccatttttc ctcctacgat agaatgtagc
     1741 gcgacttatc gacatgtctt cccaaggctt taactgtctt tcgctatttg aagtaattga
     1801 ccttcctcca ccggaactca ctttcccgcc tttctggccc ctacgcgatt gaatgtcact
     1861 cgtatgagtt ttttcaacat aagaaataaa agagctttga gtgaattttg aaaaagtcca
     1921 tttagctata ctttttgaaa ctccactaac ctcattttca tccagaggcg ctgcaaattg
     1981 cagattatag gcgctggcgc gttcataaca ggcttgcagc cactggtcat actcaggcca
     2041 gccctgacgg atagcgcggt atgcccattt gcgggtttta tcgaacaggg tgcagttacg
     2101 gcctaagccg tagtcggcaa cgatttcttt gtcattcgca gcgctcaggt cgagaaagtc
     2161 ggccagccag tccagcgtgt agagttccgg ctgccataca gcaattttcc agtggccgtg
     2221 gttcggattt ttgcagatta gtcccgaata ccccgcgtca gcctctaatt ttttacgcag
     2281 cgcgttctcc acggcggcgg cgtagcgcag cggcttcatt tttccgtccg gcgcggtacg
     2341 tacagaggtt ttcagcgcgt acagcaggtg cgcgtgtccg ttgtcggggt tggtgatcgt
     2401 cagtgtcggc gcgggggcat tgcggtcgct ccagtcaata gcggcaccgg cgcgatcgac
     2461 gtcgaatccc agccagaaca tggcgtgagg ctgattgaac tggatgtatt tcgccagaat
     2521 ggcgcgttcc ttaccggcaa tgcgtacacc aaagtgcaga tcgtccgaaa agtacggctt
     2581 gtgcggtaag cggtcgttga aaagttcgag tactgcgtta ttcaattcgc cctgcgctta
     2641 tggcgggctg ttgtctggca cgtttcatgt gctaatatcc tttcaaggtt tcgacctggc
     2701 cctggttatc atgcgatccg ccaagatatg acatgatttc cggggctttg ttttttctgt
     2761 tgccgttaaa ttacacgcga acggcctcgc ctgtcgaatc cttctgtcac aacggcttag
     2821 tgcgccgcca gttcctggaa gaatttcggg ttcgcctgga tggttgccag taccttcgcc
     2881 gccagtccgg tcgggttacg gcgtccggtt tcccagctct tgatggtgtc gaggctggtt
     2941 cccagcactt tcgccatctc gctctgagat acgttcagct gtgccctgat ggctttcacg
     3001 tctgccagct cgtaacgggt gacgcgggca ggctctctga cgccgttttt gatttcaacg
     3061 gcttcttcca gcgatgcttt cagctcgtca aaaaaactca tgtcacacct cgtctttcag
     3121 ttgttgggtc agcgtcttca gcgcggcttt ttccgcgtcc gtcaggctgt ctttggtgct
     3181 tttcgggtag gccatcacca gatagatcac ctcggcggtg gccagaaagt aaatcaccct
     3241 cacgctgccg ctttttccct ggttgcccgt cgccatcctg attttgcgca gcccgccggt
     3301 tttctgaatg agatcgcctt tgtccggcga ctcaatcagc gtcttttgca gctccctgag
     3361 ttcttcatcc gtggcgatct gttttatctg acgggtaaac atcggggtct cgataaactc
     3421 aatcgcgtga ctcacggctg tctcctgttt tcaatggcgt acatagtaca cttaattctg
     3481 attccggaaa agccgtcaca tagagggacc atagtagcgg gtcggttttt tctgcggctc
     3541 tggctgctgc tcacgcagct tctcggcgta ggcgcggtca tgctgcctga tcagcgcatc
     3601 aagcggctga cgttcctgct caagctctcg acctgcgcgc gcaagctgtc cacctgactg
     3661 ctcaagtgct gacacaccct gtcgctgtgc gccagcgcct gtgaggtagc ctcgaacatg
     3721 tgctgcaaac tgctgtagct gtcctgccac gcctgctgct gttgctcgta gcctgtctgt
     3781 aagctctcta aggcgctcag aagctgtttt tccatctcgg tcatggtttt ggtcgtcctc
     3841 agtgttaaaa acgcgctgtg ccgtttcagg gcgctctgga ggggttattt cgccgtgtat
     3901 ttcaccacgg tgttgtcggt cgtcgacgtg taggtttcca cgccgtgcgc ctgcttcggc
     3961 agcaccacca ggtaggtgtt ccggcgcgtg tcgtcgctgt aaatcgtcac gcccgcgccg
     4021 cttttcgctt tcaggttcgc cagctgctgg ctgtagctgt ccatctcgct caggcgcgag
     4081 gtgatgatcg tgccctgaaa ccagattatc ccgcccatta tgccgatcag cagggcgaag
     4141 cagatgaata tccacgccat tgctgttgag actgacttcc tgagcatgcc cgtcagcctg
     4201 ccgttctgct cgcggatagc gttgctgatg ctctgcgagc ttgattgcag ttccgcgctg
     4261 atagacgtct ccagcctcgc gaactcgcgc ttcacgctct gctcggtatc ctgcgcctgc
     4321 tgcttcgatt tctcctcgaa gcccttcgcc aaatttaaaa tctcgctcat atatcattcc
     4381 tttcagccgg atatttcggc cgccgtccgg gtcggcaatg ctgatgctgc ttttcgtttc
     4441 ccgcgccacg gtcaggccgt agctttccag ctgcgtcacc acgtcctgcc ggctgcggat
     4501 cacgccctgc tgcatcaggt tcatcaggcc ggcggtgatt ttttctgccg cctgctgttt
     4561 gtcgcgggga agatcgctgg cctgcgtcag cgggcggcga taggtcgggt cgttcgggtc
     4621 gcgcagcccc agccggtcgt tggtcagggt ctgccaggcg ttgacgcggg gacggtcggc
     4681 gcggtcaaag tagggttgca ggcgttttcc gctctgcaat tcgatgttcg ggatgacaaa
     4741 attcagctca agccgcccct tgtcgcggtg ctctacccac aggcaggcgt actggtcgca
     4801 gtcaaggccg gtcagcagcg tgtgctccca ttcgtccatc agtcgcgatt tctccgcgtc
     4861 tgcaatgtcc ggctcctgaa atgacagtac gccggaggtg taagcgcggg caaagtcgca
     4921 gccgtcaatc agctcgcgca catgttcagg attaccacgc agcacgctgg cttgttcacg
     4981 ctgccggtct tttcccagca ggtaatccac cggcccgctg ccagaacccg cgccccgcga
     5041 atgaatttta acgatcacgg ttttgctcca gtaggtcgcc aagctgacgg tcgaggctgt
     5101 tcagcacgac aaacagtgag acacgttcag agggcgtcag ggaatcagcc tggttcaggc
     5161 ggcgggcaat ctggttcagg ttgttgccaa tcccgctgac ctgtcgaagc agttcgggtg
     5221 caacgtcagg cacacgacgc ttgcgtgagg tgtgttcacc cagacctagc tgacgcagcc
     5281 actccgcaag gtgagtccgg tcgcagcgat caagcagtcg ctgatgctca ctttcggtga
     5341 gccggatttt gatctctttc gttcgctttt ccataggctc cgctgagaaa aaattcgcac
     5401 caccgtgaga atttttcgca gtggatgcca ggggtttcgg ggggcggcga gccccctgaa
     5461 ccagtcacgg tggcacctcg ttagagggga cgcggtgtgt actggcttag ggtaaagtgc
     5521 agtatgtcag attcagtgct ggctgcaaaa aaaaaagccc tcttagcaga gggctgttat
     5581 ctgatatagg cttgttatgg tctcactctt gattaatcag gtgcatgagc agcctcggcg
     5641 ctcactatcg attatgcagc ttgattatct gaggtatctt cggttcgttt tttgtctgtg
     5701 atggattcgc ttttatctct caaggctgag tctatgacgt taaacacctt ttcccatcga
     5761 gaaatccaat cgcatatagg ttgaacgcga catttttcca agaagaaatt tgataagtta
     5821 gtttttttat tatcttgc
//
