LOCUS       NZ_OW970372             4167 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Klebsiella pneumoniae isolate 147 plasmid P7, complete sequence.
ACCESSION   NZ_OW970372
VERSION     NZ_OW970372.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA7082334
            Assembly: GCF_903993005.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_903993005.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:16:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,580
            CDSs (total)                      :: 5,458
            Genes (coding)                    :: 5,322
            CDSs (with protein)               :: 5,322
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 51 of 136
            Pseudo Genes (incomplete)         :: 84 of 136
            Pseudo Genes (internal stop)      :: 17 of 136
            Pseudo Genes (multiple problems)  :: 14 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW970372.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /isolate="147"
                     /isolation_source="Hospital Univeristario de Bellvitge
                     (Barcelona)"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2017"
     gene            complement(join(4061..4167,1..7))
                     /locus_tag="LQV23_RS27940"
                     /pseudo=""
     CDS             complement(join(4061..4167,1..7))
                     /locus_tag="LQV23_RS27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            423..614
                     /locus_tag="LQV23_RS27740"
                     /old_locus_tag="AI3000V1_5267"
     CDS             423..614
                     /locus_tag="LQV23_RS27740"
                     /old_locus_tag="AI3000V1_5267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            614..1015
                     /locus_tag="LQV23_RS27745"
                     /old_locus_tag="AI3000V1_5268"
     CDS             614..1015
                     /locus_tag="LQV23_RS27745"
                     /old_locus_tag="AI3000V1_5268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1361..1603)
                     /locus_tag="LQV23_RS27750"
     CDS             complement(1361..1603)
                     /locus_tag="LQV23_RS27750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1969..2601)
                     /locus_tag="LQV23_RS27755"
                     /old_locus_tag="AI3000V1_5269"
     CDS             complement(1969..2601)
                     /locus_tag="LQV23_RS27755"
                     /old_locus_tag="AI3000V1_5269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(2601..3716)
                     /locus_tag="LQV23_RS27760"
                     /old_locus_tag="AI3000V1_5270"
     CDS             complement(2601..3716)
                     /locus_tag="LQV23_RS27760"
                     /old_locus_tag="AI3000V1_5270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     Typing          complement(2..302)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.046"
                     /coverage="99.0"
                     /evalue="1.77e-121"
     Typing          complement(4062..4167)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.393"
                     /coverage="100.0"
                     /evalue="2.31e-38"
ORIGIN
        1 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
       61 tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
      121 gagcgcacga gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      181 cgccaccact gatttgagcg tcagattccg tgatgctcgt caggggggcg gagcctatgg
      241 aaaaacggcc gctgtgcggc ctttccttct ttcctccatg cgattactcg gttttctcga
      301 gcgtttctac gcatgctcct gccgctcgcc gcagccgaac ggcaggagcg tagcgactga
      361 gtgagcgagg aagcggaaaa gaggagatcc ctacactgac cactgacgct ctgaggcctc
      421 ttatgacgaa acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc
      481 tgctcctgct ggagaagctt aacgagcttg atctcgatgt cgaggccgac ctttgtgaga
      541 aactccacga tgatgcggag catcttttcc gtacactgtc ctcgagactg gatgcattac
      601 aggatggtaa ctgatggcca gagtgtctat tagtgaggct gcccgactga cgggtaagag
      661 tagaacaaca ctgcacaggt taattaaagc aggtgacctg tccacctgta gcggtgagcg
      721 caatgcaaaa atggtagata cttcagagct tttacgtgtc tttggcccct ttgagcaacc
      781 gaagggtgaa caccattctg gacaggtctc tggacagcgt gatacagcct ctgctgacca
      841 gagtgaacag gtgatccgtc atctgaaaca ggagattgaa cacctgaaca ccttactttt
      901 gtccaaggat tcgcacattg acagcctgaa acaggccatg ctccttcttg aaaatcacca
      961 ggcaaaatta actgaatccc cggcgccatg gtggaaattc tggaaaaaat cctgaccccg
     1021 tcatgcttca gttttcctct ccggccatcc cagggagttc ctgaaccttc ccaaaaatcg
     1081 tcagatctct gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaaaatt
     1141 ttgcagattc cttatttaca acaatgggtt aatgaaaaat aaatgtgcgg tgatgtgtta
     1201 caaagtgcag atccaagtcg tggacgactc tatggcgaat cgctgacgaa ttgcccacga
     1261 ttcatatgtg aatcacgctt tattatcata ttgttatgat gaatcgactc ttgattcaat
     1321 tcatcacttt gggtgttttt tgctcatggt gactcttgat tcaattcacg attcgaatcg
     1381 ccggtgaatc gtgtggtgag tcgtccggcg acttgccata acgtgattgc cggccgtcga
     1441 cgaacccgat tcctgaaggt gctgaaaacc ggttttccgg ctgcgccggc aacgcttcac
     1501 ggccacgcaa taaattgcgt ccccgttcac cgttttcgga ggagaagaac gccggaaaca
     1561 ggacttcccc tggctgaatg gtgtgaattt ccgcgctatg cacttgcgcg catactcatg
     1621 catgccgtaa aaacagagcc tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt
     1681 tttaccggtt tcccgtcaga aacgtcctga gtccgtttta gcggtgcgta caattatgat
     1741 tttatgttaa attgttttaa gtgaatagag agaatataac ttaagggagc atcttgtgct
     1801 gggtctttta cattagatct gcacaatcat tgttttggca tctggtgatg tctttaggac
     1861 gtttgagatt gctgataact aaagtgtatg atgctttcta tacgttatcc ggcgtttaat
     1921 aacaccctgg tgataagcca gcccgtgaag ggctggcttg atttggattt atatttttga
     1981 aataaaatct tttatttcat caaagcaatt gtctgctgtg tttattattc catttttcat
     2041 ggatattcct attcttgagt cggattctct attgcagcta agatagactt gtaccttagc
     2101 ttttttttcg tttgttggat aagtgtactc tttgtcactc gctgagatgt catctttatt
     2161 atttttaatt ttttcgaaaa attctattat gtattcatat aacgctcgag tgcttgcgtg
     2221 ttcaattatc agatcttcaa tggcaccttg cttgtctttt cctggcatta taaagaaaga
     2281 aatttttgta ttgccattca tagctatctc gttatgcgag ctgggggagg agatttgtag
     2341 ttctcgcaat gtggcaatta ttgatctttc tgtgtcagaa aatgatgtgt cagcatctaa
     2401 tataataaaa atactttctt tttcatcgaa agaatcaatg tttttgaatg ctctaagtgc
     2461 ttgttttaat tttgattttc catttatttt ttcgacaaaa acatcggtaa tgccttgtat
     2521 ttcaagtagg ttgcatagaa aatgaaaatc atcatcaccc tcaacgaaaa tgatcttttt
     2581 gctattattt atgtctttca ttatcttacc tcccagtcgt tctcaactga gtaggagaaa
     2641 tcatccatat tgaattgtac ggctgttggt tttagggagt tcttttctct tcctagtcta
     2701 atataagcga tatcttcatt tttgctatct atttcgttta ttgctcgtat cacatcttga
     2761 ctatgagttg ttgcaaatat ttgattgttg ttttgttttg cagatgaaat taatgatttt
     2821 attatgtctt taattactga gtaatgtatt ccgttctcga tttcatcaat aagaataatt
     2881 gagttttcct gtaccagcac tgagctgata aaagataatg ctcgactgat accctcgcca
     2941 agcattgata tttcatttag ctcactaaat ccaatgtcta aatatatttc cttattgtta
     3001 cctatcgcag atattgctat atctacaatt ctgtcatcta ttttttttaa gttttctagt
     3061 atgtcttttt tccttttttg tgttagtagg ctagatacat ttgtaattgt tgcctctttg
     3121 tttatctttc tggatgttgt tattattgtt gctgtcttaa atttataatt aatcccatcc
     3181 ctttcgtttt ttatttcgga ttttatttct acgccatctt ctttcaattg catagagaaa
     3241 agcttattgg aaaccctgtt tttcgcattg ggtgtctcgg tcacatttat ttttaaagag
     3301 tttactgtgg cctgagaacc gatagtttga cgaggtatgt tttggttaga tagtacggtg
     3361 tcaacgttta gagacaaact tgattctaat ctctgatttg tttctgtttc atatgtttgt
     3421 gttacttcag agcgttcatc tcgaatgact atggacatct tttgtgatgt gtccatatca
     3481 tgaaagtaac taacccaaaa tggcttgttt ttgtttattt ctgccatttc ttttctgaat
     3541 gcttgaatgt ttaacaaaac agccgggttt tttacatcat aaaaacaaaa aatagcatcc
     3601 agtatagatg ttttcccgta gttgtttttc ccagctatga tatttaaagt ctttaattgc
     3661 tttagatata gctcattgaa gcatttgaag ttctttaaac ttatttcctt tatcatgata
     3721 gcctctgttc gtgtttttgt tatatgccat acgatttgtt agtaccttaa taaaatgtac
     3781 agctttcatt gtgattagct agactttatg tcaaagatta actttgattg cttgtgtttt
     3841 aatcgatttt ttttactttc ttcttgagat tatctggcag cagccgtaag cttccggctg
     3901 tgaacgaaaa aaaccgcctt ggcgggcggt tttttcgaaa ggttcagtag agttggcgct
     3961 ctatgaaccg aggtaactgg cttaaaggga gcgcagtcac caaaactgtt cgtttagttt
     4021 agctttaact ggcgtataac ttcaagacta actccctaaa tcagttacca gtggctgctg
     4081 ccagtggcgc tttgtcgtgt ctttccgggt tggactcaag acgatagtta ccggattagg
     4141 cgcagcagtc ggactgaacg gggggtt
//
