LOCUS       NZ_OW969915             4805 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Klebsiella variicola isolate 0 plasmid P3, complete sequence.
ACCESSION   NZ_OW969915
VERSION     NZ_OW969915.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8158484
            Assembly: GCF_905335825.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella variicola
  ORGANISM  Klebsiella variicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905335825.2-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:25:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,626
            CDSs (total)                      :: 5,504
            Genes (coding)                    :: 5,367
            CDSs (with protein)               :: 5,367
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 60 of 137
            Pseudo Genes (incomplete)         :: 82 of 137
            Pseudo Genes (internal stop)      :: 24 of 137
            Pseudo Genes (multiple problems)  :: 24 of 137
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW969915.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Klebsiella variicola"
                     /mol_type="genomic DNA"
                     /isolate="0"
                     /isolation_source="Hospital de Parla (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:244366"
                     /plasmid="P3"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            join(4520..4805,1..278)
                     /locus_tag="LQ196_RS28060"
     CDS             join(4520..4805,1..278)
                     /locus_tag="LQ196_RS28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_181586209.1"
                     /translation="MRALSVPPSVARANLASKFSSHLKAIALFNTMQDSQVVILSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTVQTVYFIE
                     PEHIEQLYADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIDERFTHMLDELNAA"
                     /name="hypothetical protein"
     gene            complement(307..768)
                     /locus_tag="LQ196_RS28065"
                     /old_locus_tag="AN2340V1_5373"
     CDS             complement(307..768)
                     /locus_tag="LQ196_RS28065"
                     /old_locus_tag="AN2340V1_5373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042948703.1"
                     /translation="MSKQNYIQLRSLSDQFYADNKGLQEALDGSNDGKVRGYGIVVIDL
                     NGLMFGIPLRSNLNHKFGFISERSEGNKKGLDYTKALLIQKEEYVSRAYKIPTPEFTHL
                     NDNKEKIQKEFKKFIAKYIDAHNKNDTNILRNYRYSTLKNYHKELGIED"
                     /name="hypothetical protein"
     gene            complement(1157..1318)
                     /locus_tag="LQ196_RS28160"
     CDS             complement(1157..1318)
                     /locus_tag="LQ196_RS28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_227502736.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHAKAPLAAATGRQDVREMYL
                     EFLKWWPN"
                     /name="replication initiation protein"
     gene            1733..1924
                     /locus_tag="LQ196_RS28070"
                     /old_locus_tag="AN2340V1_5374"
     CDS             1733..1924
                     /locus_tag="LQ196_RS28070"
                     /old_locus_tag="AN2340V1_5374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042948710.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLTSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1924..2325
                     /locus_tag="LQ196_RS28075"
                     /old_locus_tag="AN2340V1_5375"
     CDS             1924..2325
                     /locus_tag="LQ196_RS28075"
                     /old_locus_tag="AN2340V1_5375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_230138785.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLRGEHHSGQVSEQRDTASADQSEQVIRYLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQEKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3168..3377)
                     /locus_tag="LQ196_RS28080"
                     /old_locus_tag="AN2340V1_5376"
     CDS             complement(3168..3377)
                     /locus_tag="LQ196_RS28080"
                     /old_locus_tag="AN2340V1_5376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_042948706.1"
                     /translation="MKSIKTFVVVTALSMMSFGAFAQSISATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            3853..4263
                     /locus_tag="LQ196_RS28085"
                     /old_locus_tag="AN2340V1_5377"
     CDS             3853..4263
                     /locus_tag="LQ196_RS28085"
                     /old_locus_tag="AN2340V1_5377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042948707.1"
                     /translation="MAFISLSNLAAKTGRIIQSQHVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEDENKKLLSDDSFTQLSEGVIIFKLDDE"
                     /name="hypothetical protein"
     Typing          complement(1211..1612)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.086"
                     /coverage="98.0"
                     /evalue="5.97e-166"
ORIGIN
        1 ctgtacaaac tgtctatttc atagagcctg aacatatcga gcaactttac gctgatcctg
       61 agcttgtctt tgctaatcaa gaaaggagcc ttttctttcg ttctctcgaa aaggaaggaa
      121 agaatcttgg taagcttatc gagaagaaag gcagtgtttc tcaggcagtt ctaagtcttc
      181 tacatcatgc ttacaaagac aaacctcttt ctgacgagat gtggaaagag atagatgaga
      241 ggttcactca tatgttggat gagttgaatg cagcgtgaag gtaagggcgg tctttcgacc
      301 gcctttttag tcctctatcc ctaattcttt gtgatagttt ttcagcgtag agtaacgata
      361 gtttcgcagg atgtttgtat cgtttttgtt gtgagcgtct atgtatttag ctatgaattt
      421 tttgaattct ttttgtattt tttctttgtt atcattgaga tgagtgaact ctggtgttgg
      481 tatcttatac gcccttgata catactcttc tttctgaatt aacagcgcct tagtgtaatc
      541 caaacctttc ttattcccct ctgaacgttc agaaatgaac ccaaatttat gattcaaatt
      601 gcttcgcaag ggaattccga acatcaaccc gttgagatca ataactacga tcccgtaacc
      661 tcttactttt ccatcattac tgccgtcgag agcctcttgt aggcccttgt tgtcagcgta
      721 gaactggtcg gaaagagacc ttaactgtat gtaattttgt ttgctcatct ttaatatcca
      781 ttggcatttt ctttttacca atgtacaaaa aaagcaaagc cctctgtaag agagggcttt
      841 gcttttcttt cgttgagtct tggatttttt acgtgtggcg aactctaccg cacatctttc
      901 tttggaagct tgtttttttt acgtggggct cattcctacc gccacttctt gagttcttta
      961 tatcaaaatt tataggggaa gtcaatttag tggctgttct tgactggact tacttcacca
     1021 gcgtaatctt ctcctgaaac gaaaaaacca ccctggcagg tggtttttcg aaggttattt
     1081 aatcctggca gattatttaa ccgtggtaac agtcttgtgc gagacgtgtc accaaatccg
     1141 tccttccagt gtagcctcag ttaggccacc acttcaagaa ctctaaatac atctctcgca
     1201 catcctgtct accagtggcc gctgccaatg gcgcttttgc atgtcttacc gggttggact
     1261 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcatac
     1321 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
     1381 cgcggccata acagcggaat gacaccggta aaccgaatgg caggaacagg agagcgcacg
     1441 agggagccat cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccgccac
     1501 tgatttgagc gtcaaattct gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc
     1561 cgcgtggcgg cctcctcttt tcccctacct tgatcgctcg gtcttctcga gcttttatat
     1621 gcatgctcct gccgctcgcc gcagccgaac gaccggagcg tagcgactga gtgagcgagg
     1681 aagcggaaaa gagactggtt ttacattgag cactgacgct ctgaggcttc ttatgacgaa
     1741 acaacagaaa acagcgctga acatggctaa attcatccag catcagtctc tgctcctgct
     1801 ggagaagctt aacgagcttg atcttgatgc tgaggctgac ctttgtgaga aactccacga
     1861 tgatgcggag catcttttcc gtacactgac ctcgagactg gatgcattac aggatggtaa
     1921 ctgatggcca gagtgtctat tagtgaggct gcccgactga cgggtaagag tagaacaaca
     1981 ctgcacaggc taattaaagc aggtgacctg tccacctgta gcggtgaacg taatgcgaaa
     2041 atggtagata cttcagagct tttacgtgtc tttggtccct ttgaacaact gaggggtgaa
     2101 caccattctg gacaggtctc tgaacagcgt gatacagcct ctgctgacca gagtgaacag
     2161 gtgattcgtt atctgaaaca ggagattgaa cacctgaaca ccttactttt gtccaaggat
     2221 tcgcacattg acagcctgaa acaagccatg ctccttcttg aaaatcacca ggaaaaatta
     2281 actgaatccc cggcgccatg gtggaaattc tggaaaaaat cctgaccccg tcatgcttca
     2341 gttttcctct ccggccatct cggggagttc ctgaaccttc ccaaaaatcg tcagatctct
     2401 gaacgctcct caccactaaa aatacccaca ccccacgcaa aattaaaatt ttgaagataa
     2461 ctcattaata tcagttagtt gtgggttatt aaattacgca gtattacttt ctgtaaggtg
     2521 aatccaagtc gtcggcgatt cgtgtacgac ttgccgacga ctcgtcatag agtcatcgac
     2581 gactcactta agactatcag taacttatga cgacttgact ctagagtcga atcgtcattt
     2641 tggtgttttt ttgaccttga cgactctaga gtcaagtcac gagtcgaatc gccggtgaat
     2701 cgtgtggtga gtcgtccggc gacttgccat aacgtgattg ccggccgtcg acgaacccga
     2761 ttcctgatcg tgctgaaaac cggttttccg gctgcgccgg caacgcttca cggccacgca
     2821 ataaattgcg tccccgttca ccgttttcgg ataggaagaa cgccggaaac aggacttacc
     2881 ctggctgaat ggcgtgaatt ttcgcgctat gcacttgcgt gcatactcat gcatgccgta
     2941 aaaacagagc ctgcgcgttt ctggcggatt ttcgggtggt ttgttgcctg ttttaccggt
     3001 ttcccgtcag aaacgccctg aggccgtttt agcggtgcgt acaatcaagg ctttatgtta
     3061 aatgacgaac gcaaaaaagc ccccgaaggg gccagagggt tttcctgtta aggggcgaaa
     3121 cgggccgcct tccggcgacc tgttcggcag ctccctctgc gaccatctta tttggtcagt
     3181 tcagccgtca tgtggacacg gttattgaac tgcgcgctgg tgattttgta tgacgcaccc
     3241 tgttcagcgg cctgtgcggc aatttttgct tcagcacgat ccaaggtgga ggcggtagcg
     3301 ctgatgctct gagcaaaggc accgaaagac atcatggaga gagcagtaac tacaacaaaa
     3361 gttttgatgg atttcatggt catattcctt aagtcatttt gttctgtatg tggctttgtt
     3421 gccctgatgt gataaatgat aggacgacca tgagctgatt aaaatcgaaa caatttgcgg
     3481 atgtcattca aaaaaaaaga aagaaagata gaacgtctcc gatctctggt tcttatctac
     3541 cggccatcga ctagatgcct acgactgagg cccgatatga aggatgtttc atggctattt
     3601 agtggttttt ctcgatgttt acgcattgag tgtttttaaa tctaaaagtg aaaatagtct
     3661 ttactaacat aaagatcgat ctcgaatcgt accttgacga gtgttgatac gagcataaat
     3721 tctgtttttt ctgtttttga tgtttttttg ttggatctcg attgggagaa aatacgaaat
     3781 ttcttgaaga tcgagtgggt ccctgggtac actatcgatt aagaactgat cgacgtctaa
     3841 acggattgca ttatggcttt tatatctcta tcgaatttgg cagcgaagac tggaaggatc
     3901 attcagtcgc agcatgttac ggccacgcaa acgaaaaaaa gcactggcgc cgtagagtta
     3961 ggcttccgct taagcaaaag cgctatggac gagaccggaa tcaccattgg ggatcgagtc
     4021 gatattcttt acgacaaaga agaaaatcag tggatgatta agaaaattag tgaaggtggc
     4081 tttggtgtgt ctgggcaaaa aaacaagcac ggtggttact catcatgtgc tgttaggttg
     4141 accttacgcc ctggatttcc tcgcctttcg gaggatgagg atgagaacaa aaagttactt
     4201 tctgatgatt cattcactca gctaagtgaa ggtgtaatca ttttcaagct ggatgatgaa
     4261 taggaaagac caaaaggaaa ggcagagtta gtgagcgtcg gcaaacaatc aactaactcc
     4321 gccttctggt caacacatca ctacctttgg aagggtggcg tgttatgggc ggcattatgt
     4381 ccataaactt gcgttctaat aaagaagaaa tcgtttcttt tgaggggggc aaacacgaca
     4441 ttttctattc tgttaggtgc ggtgctattg gctttggtct cttaacgtta tgtttttaaa
     4501 cacatatgag actatagata tgagagcatt atcagtacca ccttcagttg ctagggctaa
     4561 cctggcaagt aaattctcat ctcatttgaa agctattgcg cttttcaaca cgatgcagga
     4621 ctcccaagtt gtgatcttgt catcgctgat cgagggacat cacctcacca gttctggtaa
     4681 cagtgtcaaa gctgactttg aagtcactcg cttaccagcc atcattgaga tgttagagaa
     4741 aaaatatttt ttccctattc gtcatctaaa tgtctctgtt aaatctgtaa caacaggaag
     4801 aatga
//
