LOCUS       NZ_OW969882             2317 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii isolate 22 plasmid P7, complete sequence.
ACCESSION   NZ_OW969882
VERSION     NZ_OW969882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8158487
            Assembly: GCF_905335685.2
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:50:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,913
            CDSs (total)                      :: 5,791
            Genes (coding)                    :: 5,647
            CDSs (with protein)               :: 5,647
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 32 of 144
            Pseudo Genes (incomplete)         :: 110 of 144
            Pseudo Genes (internal stop)      :: 20 of 144
            Pseudo Genes (multiple problems)  :: 18 of 144
            Pseudo Genes (short protein)      :: 1 of 144
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW969882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /isolate="22"
                     /isolation_source="Hospital Puerta de Hierro (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            complement(join(2187..2317,1..106))
                     /locus_tag="LQ207_RS29515"
     CDS             complement(join(2187..2317,1..106))
                     /locus_tag="LQ207_RS29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            868..981
                     /locus_tag="LQ207_RS29520"
     CDS             868..981
                     /locus_tag="LQ207_RS29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1362..1898)
                     /locus_tag="LQ207_RS29525"
                     /old_locus_tag="AI3059V2_5759"
     CDS             complement(1362..1898)
                     /locus_tag="LQ207_RS29525"
                     /old_locus_tag="AI3059V2_5759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     Typing          complement(337..515)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.066"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          492..981
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 gcctgttggg ctgattcagc ttcttgcctg acgctctctc agcggcggca acagcatcgt
       61 cgagaaatct tttctcggca ggcgtcaggt ttatcagtcg tttcatggcg ttctccggtc
      121 agaataactc tgcacagtct gccagacacg gcgaacctca tgaagaacct gcagccggga
      181 acggccctga gaaacggtga acgggaacgg attttaatcc ggtgccgtga agcgttggcg
      241 gggtatcggg gcgcagccct gagatatcgt tttggtgccg gcatcatgat ggcggtactc
      301 caaaaacaca tcttgtcccg ctttcagttt ctgaatttgc gggttccggg cgcagcgccg
      361 gaccagtcac gtagcgctag cggagtgtat actggcttaa tatgttagtt gggcgtggtg
      421 gctcagggaa gtgcttcatg tggcagggga aaaatgcggc tgcggtgcgt aagtgcaaaa
      481 tgctgatgat ggtgaaatga cgcctcctcg ctcactcggt cgctacgctc ctgccgtgag
      541 actgcggcgg gcgttacctg ctcacgaatg acgaggtaac gaaatgaagc tgaggtcaag
      601 aagggcccga aaaaggatgc ggcgctgccg tttttccata ggctccgccc ccctgacaag
      661 catcacaaaa tctgacgctc aaatcagtgg cggcgaaacc cgacaggact taaagatccc
      721 aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
      781 gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg
      841 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
      901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacatgcaaa agcgccactg
      961 gcagcagcca ctggtaactg atttagagga gatagtcttg aagtcatgcg ccagttaagg
     1021 ctaaactaaa cgaacagttt tggtgactgc gctcctctaa ctagccagtt acctcggttc
     1081 aaagagttgc tgctctgaga accttcgaga aaaccgcctt gctgggcggt tttttcgttt
     1141 acagagcaag agattacgac gcaacagaaa ggatctcaag aagttccttg ctgtattgct
     1201 actatcctag agatctgacc cattaggggc attatcagat gacggccttg taatccacgg
     1261 ggagcaggtc acacatctaa agcatctacg agtaaaaaca ccccagtgac caatgagcga
     1321 tttgcgtgag ctaatcgtca atgtatactg atcgcttcaa attaatcgct catgctttta
     1381 atgaaatcat ctaagtacca agccttagag aaatcactaa gatcttccat ctgttttccc
     1441 attacaccca gaagcccacc ctcccaaaca ctataagctc tctctccatt taacatatac
     1501 acgttttccg gaattgttct taatgtatat acggggtttt gaataccata ttcctgccca
     1561 agagttttaa aatactctcc ttttaagtaa aacttatctt tccctctgta actaagataa
     1621 accttactaa aatcataatc tttcactttt tcagcaaatt gcagcatcac cctactaaca
     1681 tccagtgaac tattttctgc agaaacagaa cgaaggtcat aattaatctc ggaagagttt
     1741 acaaaccact tataataaac ccaaacgcta ataccatcat tccttggatc ttctttcaga
     1801 acctcattca tgtgcctttg aagaaaaatg tagttggcag ataaaacacc accacataag
     1861 agtactatta atatgattgc tgtatatact tttttcattt ctataatcca tctcaactgc
     1921 tctaaagttt accctgagat ttttaagcta aggtgccata agttagcaag gaattaagca
     1981 tcgatttacc ataacgtctt cttacgcgca ccgcgaaaac ggcctcaggg cgtttctgcg
     2041 ccttcagcca caaaagagca cgtcttaccc ccagaaaacc gcccggaatc gctctggtga
     2101 gttttacggt ggcgaatgac tatgcaccgg agtgaatacc acgctgtttc gccctgaact
     2161 ccccacaaaa ccgacgaaaa tggcaatcag cgacgcggcg cccgcggacg cgaccaggta
     2221 aattccgcct gctgccgctc ctcctcacgc gcagatcgct gccgttctgc ctggcgctgc
     2281 gattcgatct gtgcacgagc cctgttaagg acaatgt
//
