LOCUS       NZ_OW969783             4665 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Klebsiella oxytoca isolate 328 plasmid P2, complete sequence.
ACCESSION   NZ_OW969783
VERSION     NZ_OW969783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8158514
            Assembly: GCF_905338025.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905338025.2-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:14:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,406
            CDSs (total)                      :: 5,290
            Genes (coding)                    :: 5,216
            CDSs (with protein)               :: 5,216
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 39 of 74
            Pseudo Genes (internal stop)      :: 22 of 74
            Pseudo Genes (multiple problems)  :: 18 of 74
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW969783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /isolate="328"
                     /isolation_source="Hospital Arquitecto Marcide (Ferrol)"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="P2"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(242..565)
                     /locus_tag="LQ195_RS26980"
                     /old_locus_tag="AN2363V1_5263"
     CDS             complement(242..565)
                     /locus_tag="LQ195_RS26980"
                     /old_locus_tag="AN2363V1_5263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1623..3179)
                     /locus_tag="LQ195_RS26985"
                     /old_locus_tag="AN2363V1_5265"
     CDS             complement(1623..3179)
                     /locus_tag="LQ195_RS26985"
                     /old_locus_tag="AN2363V1_5265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589620.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_032993376.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(3525..3740)
                     /locus_tag="LQ195_RS26990"
                     /old_locus_tag="AN2363V1_5266"
     CDS             complement(3525..3740)
                     /locus_tag="LQ195_RS26990"
                     /old_locus_tag="AN2363V1_5266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3744..4229)
                     /locus_tag="LQ195_RS26995"
                     /old_locus_tag="AN2363V1_5267"
     CDS             complement(3744..4229)
                     /locus_tag="LQ195_RS26995"
                     /old_locus_tag="AN2363V1_5267"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012565228.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(679..852)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          776..1328
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(2..252)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="97.0"
                     /evalue="9.38e-46"
     Typing          complement(249..565)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="1.81e-61"
     Typing          complement(3422..4665)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.084"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccgggcatc agaacccgct caaagcttgc catcagcttt tcccgctggc cggaaggtaa
       61 atccgcttcc gcaaacgaca gtaccccgga ggtgtatttc ttaacgtaag gcgacgcgtc
      121 aatcagctcc cggacttctt ccggcttccc ctgcaggacg gtggcgcctt cacgctgccg
      181 gtctttaccc agcagatagt ccaccggccc ggcgccaccg ccgcgaccgc gtggatgaaa
      241 tttaacgatc atcgtccgtt cctttctcaa gaacggcgtg ccgcagccgc tcaagtccgg
      301 catcgatggc catcagcgcc gcaacaatct gcaccctgtc atggccggta ccgccgccgg
      361 cgttaacacg gcgggctatc tggttcaggt tattgcccat gccggcaagc tggcgcagca
      421 gcgccggcga gattgagggg agctttccgg aacgtgccgg cttttcgtcc aggcaggtct
      481 gccgcatcca cgccgccagc tgcctgccgt cacagcgctc aagcagccgc cggtgctcgt
      541 cctccgtcac ccacatggtg agcatcttgt tccgtttctc tgacaaaccg gtctcctgat
      601 aacgccggga cgggcgggaa aacctttccc ggtcggcacg gggttggaca agccgcaggc
      661 gcgtcaggct tttggcgggt ttcggggcgc agccctgaac cagtcacgta gcgctagcgg
      721 agtgtatact ggcttaatat gttggcacgg atgagggtgt cagtgaagtg cttcatgtgg
      781 caggagaaaa aaggcggcac cggcgcgtca gcagaatatg tgatacagga tatattccgc
      841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tgccggctta
      901 caggtggggc ggaaatttcc tggaagatgc caggaaggaa cgtaacaggg aagcgagaag
      961 gccgcggcga agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1021 cgctcaaatc agtggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccctg
     1081 gtggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     1141 tatggccgcg tttatctcat tccacgcctg acactcggtt ccgggaaggc agttcgctcc
     1201 aagctggact gtatgcacga accccccgtt cagcccgact accacgttgt cccggtaact
     1261 atcatcttga gtccaacccg gaacagacac gaaaaatcgc cagtggcggt agccattagt
     1321 aagaggattc acggagcgga gtagtagagt gttctacaga gttcttgaag tggtggcctg
     1381 actgcggcta cactggaagg acagttttgg tacctgcgct ccgtgaaagc cagttacctt
     1441 cggaagaaag agttggtagc tctgaattcc ggcaaacgaa ccaccgttgg tagcggtggt
     1501 ttttttgttt acagggtatg agattacgac gacatcgaag ggataacaag aggatccgct
     1561 tcttgcccac tcttcgccac aaaaaaaacg acacactagc gcgtaaagca tcatcaaaaa
     1621 actcaaatag cgctatatac aatcacttta ttacgcccct cccttttggc ttgataaaga
     1681 gcatcgtcag catttttcag caaaaaatcg atcctaccaa gaggatcatc ttgtgttgcc
     1741 acaccagcag atatggtgac ccgcaagtta ttagggaaga cagtactttc aataacactc
     1801 ctaatacgct ccgcaacaca cttagctgtt accacagatg tattcggcag gaaaacaaca
     1861 aactcttcgc caccaaaccg acaaacaaca tcttcactac ggcaacagga attaataata
     1921 tgagccaaag aaacaagcac ctcatctccg accccatgcc cgaaactgtc atttatctct
     1981 ttaaaaaaat caacatcaat tacaataact gaataccctg aatcccgctt atgattctgt
     2041 attttactta aaaaccccaa tctattgttc acgccggtaa ggggatcttt aagcgccacc
     2101 tcatttagaa aatcaactct ttccatcatt accttaatat acgtaaaaag cgccttttta
     2161 agcctatcag cttcggcata ccaagcattg atagctttaa tataaactag cgaagctccg
     2221 atatctttag cattggtaga tatagcaagc ttttctaaag gaagtgaaat actggaggca
     2281 atgaaatatg aaataagtga aaacacaata attattataa ttagcagaac aagtacactt
     2341 ttagcataag caataagaat agtagtcaca ctgtcagcac gactataaac caacacattc
     2401 caagcagtat ttttcatact agcataacca agaagatatc tatgccccga agaaaaaaat
     2461 gccccctcac ctctatcaga cgcagttaac tttgatttca aatcatcaga cataaccata
     2521 ggccgatcaa cagcactttt atcgttatta aagatcacat tcccatcatc actaacaatg
     2581 ctaatttcag tgttgttctt gaaaaaatgc ctgctcaaaa catcgccaaa taaattatgt
     2641 ttttttagat atatagaccc accgacatac cctatgtaat tgccaccctt atcataaata
     2701 ggatgtgaca acaaaataat caaattccca gtaactgatt ttaacggtga agatatgaat
     2761 ggtttctttt cctcaatcgc caatttgctg gcacttgaat tcaagcgggt accaactaaa
     2821 tcaagagatt ctggcgaagt cgccagaaca acagcatcat tactaacaac aagaactgaa
     2881 ttaaacatac cagactgcaa tcttaaccta tccgcctcat ctttaagtga gtcaatatca
     2941 ttagttgatt ttattttttt tgctccatat gctaattcac cttgagccat ttcaatataa
     3001 ccactcatta catctgacat tttcatagcg tacgatgaat tagcataaag caaatctttt
     3061 tctatatttt cgcgctgaaa aaccataata acggcaatca gaaaaataga agttaacaaa
     3121 atcccaccaa cggacaaggt tatgactatc ggacgaaggt gaggcttttt aattctcata
     3181 aaaatacact ccatataata aaaaatgccg catttaccat aacatccctt atcagcaccg
     3241 ccccggacgg gttcggaaca aatgcggcgt cagcccggta aaaccggcag caaactctcc
     3301 gctaaaccgc gcattctgcg ccagataaat ttcacccggc tgcatggcta tgcagtcggg
     3361 tgaatggctc cggctttcag cgcagatgtc cgcagaaagc cccgttttat caggttatca
     3421 cagtgaaggg ctgttatacg tccgctgtcg ctgagatcgc tccagctcca gcgctttctg
     3481 atgctgcacc acgcgctcct gctcccggtg cagctcgacg gcattcagtc gctcgacaac
     3541 cggcgcagct gctcgctcaa actctgcacc tgcctgctca agcccgtgac gcgctcgctc
     3601 agcgctgcgt tctcccgttg cataagacca gacatcgtcc gccattccgc gaaggcgctc
     3661 tcccactcgt ccagcctttt cgagtagtcc tgctgtagct gctctaatgc gctcagcaac
     3721 tgtttttcca gctccgtcat gtgctatttc cccgccagta tcatccagct cgaatcctct
     3781 ccgaaccgtc ccgcttccgg gttcaccttc acgcagcggc gtctctgctc gccgcaggtc
     3841 gagagctgca cgccgctgtt cctctctgac agcatggcct gcgtgctctt ctgctcccgg
     3901 atggtcgtgt agttgtcgag tatctgctgc ccctgccacc acagaatgcc cgcgctcgag
     3961 gcgatcagca gcgcggagac caggacgatg gtcagccacg tctggctgac catgcgcacc
     4021 atgcctttcg tgcgctggct catggctgag gacagtttcc ggtcgtggtc gaggatggcg
     4081 gcgctgattc tcttcgcgct ttcgttcagt tccgctttta cagatttctc gtgctcgctg
     4141 aatgcggctt tcagcatctc gccggtattc tgctgctgcg ctttcgattt ctgctctaag
     4201 tccttcgcca gcgttaaaag actgttcata aatggctccc ttcagtcgga tgtttcgccc
     4261 cccgtccggg tcggcaatgc tgatactgct ttttgtggtg cggaccacct caaaacctgc
     4321 gctttccagc gcctcagtga catcctgacg ggttttcagc tccccggatg aggcaagagc
     4381 aagcagaccg cgtgtaatcg cttctgcagc ctcctgcttc gctttaggca gtgcagacgg
     4441 tgtgaccagt gcccgccggt tctccggtgc gttcgggtcg tgcagcccca gtctgccgtt
     4501 cacgatggtc tgccaggcat cgatgcgtgg acggtcggcg cgatcgtaat acggctggag
     4561 ccgtctgccg gtcagcagct ccgtgttcgg gatcagaaaa ttcagctcaa gccgcccctt
     4621 gtcacgatgc tccacccaca ggatgctgta ctggtcttta tcaag
//
