LOCUS       NZ_OW968294             2442 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P6, complete sequence.
ACCESSION   NZ_OW968294
VERSION     NZ_OW968294.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065812
            Assembly: GCF_905331185.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905331185.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:10:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,161
            CDSs (total)                      :: 5,040
            Genes (coding)                    :: 4,783
            CDSs (with protein)               :: 4,783
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 90 of 257
            Pseudo Genes (incomplete)         :: 177 of 257
            Pseudo Genes (internal stop)      :: 50 of 257
            Pseudo Genes (multiple problems)  :: 55 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968294.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital de El Escorial (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P6"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            join(2300..2442,1..88)
                     /locus_tag="LQ134_RS25615"
     CDS             join(2300..2442,1..88)
                     /locus_tag="LQ134_RS25615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(158..1126)
                     /gene="mobV"
                     /locus_tag="LQ134_RS25620"
                     /old_locus_tag="AI2981V1_4949"
     CDS             complement(158..1126)
                     /gene="mobV"
                     /locus_tag="LQ134_RS25620"
                     /old_locus_tag="AI2981V1_4949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            1831..2313
                     /locus_tag="LQ134_RS25625"
                     /old_locus_tag="AI2981V1_4950"
     CDS             1831..2313
                     /locus_tag="LQ134_RS25625"
                     /old_locus_tag="AI2981V1_4950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          complement(162..1135)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 taaatactca actaagggtt tgtcaccaaa gcagaaaaac gcagcttttt ctattttaaa
       61 aaaacataat taccatctga atgattaatt ttttcgtggt gaatgccgca aatgacgtca
      121 ctttgcggcg ttagccctat cctgcatcgc atcaaaacta acgctccata ccacccatgc
      181 cacgcgatcg cgtctgtcgc tgtttttcac gttcaatacg ctgctgtttt tccttttcct
      241 ggttttgctg cttaaaccgc tgagccatca tcaggatttc ggtcatctgc tctctggaaa
      301 gccccttaaa aggctctgtg acgctctgta ggcgtttctg ctgcgcatcc atagtcttct
      361 gtagctcacg cgctcttttt gcgttctggg cgctctgaga ggccatagcg atggttcctg
      421 caaagccatc gttcactgcg tggcttagac gtttggctat ggctgcctga tcttccacgt
      481 ctttggtgaa aatgcccttc cgcaatacac gcggctccag cgcctccggc gagatcgata
      541 cctggctacg ggtgccacgg tttatcgatt cgtagtagtg ctgaactgtc tgatgcgtgg
      601 cgcggcttcc ctcgatgccg cgctcaagac ccagtttttt tactgattca gcgtaggtgc
      661 tctgatcttc acgcatcttt gaccgcccgc caataaactc tttcgcggac agccgtccat
      721 cctgcgtcaa tggcaccacg aacgcagaca agtgcggggt ggcctcgtcc cggtgaacca
      781 ccgccgccac aacacgatcc ttcccgtatt tcttctccag ccattgctca gagcgggcga
      841 aaaactccgc ctgctgccgt ggtgtggcct ctttccacca ctcagggctt gctgtcataa
      901 cgtattcaac ggccagtacc gcatctttac ggcgtttttc tggcagcagc tcccgcacac
      961 gccccattgc ctgatccgtt gatttggaca cagagcagta gttttctggt gtccgttcag
     1021 cgttagcgtt cggcgtctca cgctctcggt agcagtgctg caacgcgctg gccacgcttc
     1081 ccattcccgt cagctttttg cagcgcatta tcgcgtagtt cgccatgttc ccccctgatt
     1141 ccggcattca ttcagccagg cgggggagcg cattgcgata cctccggcgg gccactatca
     1201 agatgatagt gtactcacta gactttgcta cgcaaaatcg ttcgggcagc aagctgccag
     1261 aaggtcaaaa tcaacggcgg tctggcaggt ggaaggcgtc gcttcgcgcc accccccacc
     1321 actgccaagc ctgttttatc cggagtgtcg tcaagggtaa atggcactcg ctacgctcgc
     1381 ccttgacgaa atcaggcaat gaaatcgtct ttgaaagagc aggaaccgct cgccgcagct
     1441 ttggctgagg cgtaccgcag gagcaacgcg acgaggaacg aacagccaaa gcgaggaagc
     1501 ggaatttcca ctccccgtac atagaaatgc gtacactcct tgctaaaaaa aggcagtttt
     1561 tcagtgtcta aaaatgccgt ttttcagccc ttattttccc ccttaatata tagatataga
     1621 tatgggaacc cgcttgtacc aagggctaga gcgaagaatc gtactcacct caagtaccaa
     1681 tcgtactcac ctcaagtact aatcgtactc acctcaagga ctaatcgtac tcacctcaag
     1741 tactaatcgt acttgataca aaggacttga ctgtatgtct ctttagtact taaatgcgag
     1801 tactaaatca tggcgaaacg tggggctgga gtgaatacga aaaagatttc taggcttgaa
     1861 cggcatacat acatggatga aaacggtgag gtaaaacaag actctaagat cgagaccttt
     1921 tccatcccag ccgaacctcc ctacgtcaag ctatacattg cagacattgc gatgattcat
     1981 ggtttatcaa aatctcaaaa tgatgtgctt ttacaattgg caatgctgat tggatgggat
     2041 gggtttgtgt ctgtatcaaa aactagattt gaaaaaacca tacagcccag ggttcatatt
     2101 gaatatcaga cctttaaaaa cataattgcg gctcttgtag ataaacaaat attcttccgg
     2161 gcagggcgtg gggaacttga agctaacccc tttctttttg cacggggaga ctggccatca
     2221 atttacgaaa gaagaaaaaa aattgagtta aagatcagtt atgacaccaa tggaagaacg
     2281 gtgcagacga aggtaatgga tgatgaatgc tgaagataaa ctttttaaaa atgtagacag
     2341 gctaatgctg catgattcac taaaaaagaa tgaggttctg actgtttggg agcgtacttt
     2401 tttaagcagt gtttacagca tatatcgtag aactagcggt aa
//
