LOCUS       NZ_OW968293             4074 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P5, complete sequence.
ACCESSION   NZ_OW968293
VERSION     NZ_OW968293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065812
            Assembly: GCF_905331185.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905331185.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:10:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,161
            CDSs (total)                      :: 5,040
            Genes (coding)                    :: 4,783
            CDSs (with protein)               :: 4,783
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 90 of 257
            Pseudo Genes (incomplete)         :: 177 of 257
            Pseudo Genes (internal stop)      :: 50 of 257
            Pseudo Genes (multiple problems)  :: 55 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital de El Escorial (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2016"
     gene            complement(join(2624..4074,1..709))
                     /gene="mobQ"
                     /locus_tag="LQ134_RS25600"
                     /old_locus_tag="AI2981V1_4948"
     CDS             complement(join(2624..4074,1..709))
                     /gene="mobQ"
                     /locus_tag="LQ134_RS25600"
                     /old_locus_tag="AI2981V1_4948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023308213.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGVSGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            888..1142
                     /gene="mobC"
                     /locus_tag="LQ134_RS25605"
                     /old_locus_tag="AI2981V1_4946"
     CDS             888..1142
                     /gene="mobC"
                     /locus_tag="LQ134_RS25605"
                     /old_locus_tag="AI2981V1_4946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1674..2366
                     /locus_tag="LQ134_RS25610"
                     /old_locus_tag="AI2981V1_4947"
     CDS             1674..2366
                     /locus_tag="LQ134_RS25610"
                     /old_locus_tag="AI2981V1_4947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023308214.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVEELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(352..710)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          711..887
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          1675..2366
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.815"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..796)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.491"
                     /coverage="99.0"
                     /evalue="7.68e-175"
     Typing          complement(2628..4072)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gggaaatctg tatttccagg ttaagcctgt taatcagacg attctgttcg ccgcgttctg
       61 tttcgatacc gcgtgtttcc atcgcacagg cagactttcc gaggtgaata ccaggcgttt
      121 tctcaagccc ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa ccggcgttcg
      181 ccagtgctct gtttgcatgg tcagcccatg atgcacgcca cgtctcggca tgtgtccggt
      241 cgttccagtc cctgaccttt ccgccgaacc cagcaggtcc gacagccctc gtggtcagca
      301 tgatgtgcgc atggggattt gttttatcca tgtgatggaa cgccacatca gcaatcatgc
      361 cctgactgac aaagttttct ctgacaaagg cgagaacggt ttcccgtgcg gcatcctgcg
      421 gcagttcacg ggggatagcg agttcaattt cccgcgcaag ttgtgagttc ttccggcatt
      481 cggccttctc aacggcattc cagagttcgg cacggttcgt acaccaggca ggggctgtcg
      541 cgggggtcag aatcacagct tcagccacac cgcttttgcg ggtgtagtcg tgagtcagtc
      601 cggtacgctc atcaagcatc agtgaaccgg aacgataggc agcggaagct acggcgctgt
      661 agccgctgct tctggagata attttcatgc tgagatgata aatcgccatt tctgccgtaa
      721 tgcctttttg tagttttctt tcagccccgc agggcgcaca caatcgaaac ggagtggaga
      781 ttgtataagt gcgcactttg tgatttgtcg tgcattatgc cagctcagtt tttaaactac
      841 cagtgacgcg ctaaaatgcg atttttcatc gcgcacggag ttacatcatg agcagaacgc
      901 tcgaacagaa gattgctgat gctgaggcga gattacagcg cctgaaggcg aagagccgga
      961 gcctggacac agcgcagaag gttgttgtgg gtgcagcatt gctggcaaag gtcagaaagc
     1021 cggaggaggt gcagttgcgt gcctggttac tccagttcct gaaggcagag gtgacacgac
     1081 aggctgatgt gacgcgcata ctgccgctga ttaacgagct ggaggcgctg ccaggacagt
     1141 gattgtatcg tccgctcgcc gcagccccag cgaccgagcg ttagcgagcg agtgggcgag
     1201 gaagcggaag tgaatccgtc gccaggttga gcacatacgc acggtttttc ggtaggtgat
     1261 atgcgctgaa cccgcatgaa tactggattt ccacagggtt ggaaggggag tattcggaac
     1321 ttgcaattct gctttcttgc aacacattgt ttttattggt tttttgtttt cagcgcccca
     1381 agttatccac aggctcgccg ttaagttttt agatcttgtt tttaagatct tgtttttaat
     1441 acttgtttta gggtcaaaat tcattaataa aatcaatatg ttatagcggt atatgtggag
     1501 gaatttaagg taaaggtgga ggaatttaag gtgtttaggt ggaggaattt aaggtaaagg
     1561 tggaggaatt taaggtgtta ttcacagaaa tgtgaggata taaaaaaaaa tattccacat
     1621 ttcttatttg tggggcacat taaagcatca ccacatttga acgggatctt tgtatgtcgg
     1681 acttgattgc ctacaagtcg aatgctttgg ttgaggccag ctacaagttg accctacagg
     1741 aacaacgttt tctgttgctg tgtatcagtc gattgaaatc tggtgctgat gctgaattgc
     1801 aaaagacaat gaccattacc gctgcggaat attttgattc attccctgac atggggcgga
     1861 aaaatgccga ggttcagtta caggaagcga tcgatcgttt atgggacagg tcgatcattc
     1921 tcaagaatga cgagaagcgt gaggagttcc gctggatcca gtatcgggca cagtacgcta
     1981 aaggtgaggc caaggctcga ataacctttt ctgacgcggt aatgccttat ctgacgcaac
     2041 tgaaggggca attcacgcgc gttgttataa aaaacatatc aggtttatca agtagttaca
     2101 gtataagaat atacgagttc ctccagcaat tccgcagtac gggagagaga attattgctc
     2161 ttaatgactt tcgttcaatg ttagggattg aaaataaata caaacaattc agagacttaa
     2221 ataaaatact aataaaaccc tgtgttgaag aactgaacaa aaaaagcgac ctggctgtaa
     2281 cggttgagac gatcaaaaag gggcgcacgg ttgtagccct gcattttcgc ttcaaggaag
     2341 acaaacagat caagatgacg atctaaaatc gattttaagg cacctaaaac gctccctgct
     2401 gcgttttttt tgtgttagta gtgcgaaggt actattgaag tgaaaaattg cgtcaaaagc
     2461 gctctaatgc agcgaaaaaa gaacccccaa tttttggggg cggaaaaaca aaactatgct
     2521 tgcattatgt gtgtgtgctt cttgcattca tccctgtcga tgaacatcga agaacatcga
     2581 cgaacataac acgatgttaa acaaaacaaa atattgttca cattcactgt gaccaaccgc
     2641 gatcatgtct cctctcggtg gtgtgtgttc ttgcatcttc ctcgcgccgc cattcatccc
     2701 aggtacgatt gaaacccaga gagcggaaca gatgatccgg ctgtttttct ggtgtgttga
     2761 cggcagcaaa aagcggtgcc tcacgggttt tcagatgaaa ctccataagt tccggcgcag
     2821 gtgatatgtg gctgtctgca tcgtgattta cggcaacacc ataccctgca taccgtaacc
     2881 ccttatcggt cagtgcactg atgaccagct ggctgttatc ggctgaggga acggcaaacg
     2941 cgggatacag cgtaccatta agccggacaa caccgcccag agagtcatcc acgcccatga
     3001 ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga actcattacc ggcatcagcg
     3061 tcggtagcag acgtccctgc gtaaaatcca ccttccttgc tgccagtgat ggcatggctg
     3121 gctggcggtc ttctccaaga cagaaagact ccggccatgt cttcggctgg cggagattgc
     3181 gggtatattg cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag gtcgcagagg
     3241 aataaacacg ttgcagtggt ggatgttgac cgatttcgct tcggtcacag atataaccac
     3301 tcctgtcacg cattaaatca acacgctcaa agattctggt gcggtatttc tccagctcgt
     3361 tcccccagag cgtgtattct ccgttccggt tgtgaagcag aaggtaacag gtcatttccc
     3421 cgttaccagg catgggggcc tcgccatacc ccgtaacgac tgcgctcagt acccctgaat
     3481 ccataacggc attgtcattt ttcctgagct gttccgctcg tcgctccgcg cgactacgtt
     3541 ctgtatcctc ctgctccttc cggcgcaatt cctcttcacg tttaagcaac tggactctga
     3601 gagctgcaag gccggatacc ccccactgct gccactgttt ttcaaatgcc gtctgactcg
     3661 cgggaatgtc ctgtttctcc accttccggt aatgctgtcg caatttttcc atttgctggc
     3721 tcagcgactg gatttttccc tgttcgctac ggtattccgg tgccgcccat tgcatcaggg
     3781 gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca
     3841 tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg
     3901 ccatcatcga catggcatta ctggtgagtt tccatgccgc attactgtcc ttcggcaacg
     3961 cttcgatgat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg atattcagaa
     4021 ggtcagccac acggcgggca ggctctgtca gcccgctgtt cctgagttca gtgc
//
