LOCUS       NZ_OW968279             6647 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 131 plasmid P2, complete sequence.
ACCESSION   NZ_OW968279
VERSION     NZ_OW968279.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065782
            Assembly: GCF_905330875.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330875.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:18:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,311
            CDSs (total)                      :: 5,197
            Genes (coding)                    :: 4,948
            CDSs (with protein)               :: 4,948
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 99 of 249
            Pseudo Genes (incomplete)         :: 175 of 249
            Pseudo Genes (internal stop)      :: 48 of 249
            Pseudo Genes (multiple problems)  :: 62 of 249
            Pseudo Genes (short protein)      :: 1 of 249
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968279.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="131"
                     /isolation_source="Hospital Universitario Central de
                     Asturias (Oviedo)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P2"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(6625..6647,1..211))
                     /locus_tag="LQ166_RS26425"
     CDS             complement(join(6625..6647,1..211))
                     /locus_tag="LQ166_RS26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(218..736)
                     /locus_tag="LQ166_RS26430"
                     /old_locus_tag="AI2949V1_5068"
     CDS             complement(218..736)
                     /locus_tag="LQ166_RS26430"
                     /old_locus_tag="AI2949V1_5068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<774..1472)
                     /locus_tag="LQ166_RS26675"
                     /pseudo=""
     CDS             complement(<774..1472)
                     /locus_tag="LQ166_RS26675"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1414..1737)
                     /locus_tag="LQ166_RS26440"
                     /old_locus_tag="AI2949V1_5069"
     CDS             complement(1414..1737)
                     /locus_tag="LQ166_RS26440"
                     /old_locus_tag="AI2949V1_5069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1801..1992
                     /locus_tag="LQ166_RS26445"
                     /old_locus_tag="AI2949V1_5070"
     CDS             1801..1992
                     /locus_tag="LQ166_RS26445"
                     /old_locus_tag="AI2949V1_5070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2662..2820
                     /locus_tag="LQ166_RS26680"
     CDS             2662..2820
                     /locus_tag="LQ166_RS26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            3318..3545
                     /locus_tag="LQ166_RS26450"
                     /old_locus_tag="AI2949V1_5072"
     CDS             3318..3545
                     /locus_tag="LQ166_RS26450"
                     /old_locus_tag="AI2949V1_5072"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(3542..5110)
                     /gene="cea"
                     /locus_tag="LQ166_RS26455"
                     /old_locus_tag="AI2949V1_5073"
     CDS             complement(3542..5110)
                     /gene="cea"
                     /locus_tag="LQ166_RS26455"
                     /old_locus_tag="AI2949V1_5073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            5370..5807
                     /locus_tag="LQ166_RS26460"
                     /old_locus_tag="AI2949V1_5074"
     CDS             5370..5807
                     /locus_tag="LQ166_RS26460"
                     /old_locus_tag="AI2949V1_5074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            5888..6307
                     /locus_tag="LQ166_RS26465"
                     /old_locus_tag="AI2949V1_5075"
     CDS             5888..6307
                     /locus_tag="LQ166_RS26465"
                     /old_locus_tag="AI2949V1_5075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          complement(2078..2165)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2176..2734
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7..1424)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.789"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 actggctcaa gcgttctgtc tgctcgctca agtgctgcac ctgctgactc aactgcatga
       61 cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga aaacatcttc tgcaattcca
      121 cgaaggcgct ctcccattcg ctcagccgct gcatatagtc ctgttgcagc tgctctaagg
      181 cgttcagcaa atgtgtttcc agctctgtca ctctgtgtca ctccttcaga tgtacccact
      241 ctttcccctg aaagggaatc acctccgctg atttcccgta cggaaggaca aggaatttcc
      301 tgttcccgtc ctgcacaaac tccacgcccc atgtcttcgc gttcagtttc tgcaatgtct
      361 cttcctgctt cctgatttct tccaggttcg cctgtatcct ccctccaaga taccagagcg
      421 tcccgccact cgcggtaaac aggagaaaga ctatccccag taacatcatg cccgtattcc
      481 ctgccagctt taacacgtcc ctcctgtgct gcatcatcgc ctctttcacc ccttcccggt
      541 gtttttccag cgattcctct gtcgaggctg tgaacagggc tatagcgtct ctgattttcg
      601 tctcgtttga tgtcacagcc tcgcttacag attcgccgag cctcctgaac tcgttgttca
      661 gcattttctc tgtagattcg gctctctctt tcagcttttt ctcgaactcc gcgcccgtct
      721 gcaaaagatt gctcataaaa tgctcctttc agcctgatat tcttcccgcc gttcggatct
      781 gcaatgctga tactgcttcg cgtcaccctg accacttcca gccccgcctc agtgagcgcc
      841 tgaatcacat cctgacggcc ttttatctct ccggcatggt aaagtgcatc tatacctcgc
      901 gtgacgccct cagcaagcgc ctgtttcgtt tcaggcaggt tatcagggag tgtcagcgtc
      961 ctgcggttct ccggggcgtt cgggtcatgc agcccgtaat ggtgatttac cagcgtctgc
     1021 caggcatcaa ttctaggcct gtctgcgcgg tcgtagtacg gctggaggcg ttttccggtc
     1081 tgtagctcca tgttcggaat gacaaaattc agctcaagcc gtcccttgtc ctggtgctcc
     1141 acccacagga tgctgtactg atttttttcg agaccgggca tcagtacacg ctcaaagctc
     1201 gccatcactt tttcacgtcc tcccggcggc agctccttct ccgcgaacga cagaacaccg
     1261 gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt cccggacttc ttccggatta
     1321 ccctgaagca ccgttgcgcc ttcgcggtta cgctccctcc ccagcaggta atcaaccgga
     1381 ccactgccac caccttttcc cctggcatga aatttaacta tcatcccgcg ccccctgttc
     1441 cctgacagcc agacgcagcc ggcgcagctc atccccgatg gccatcagtg cggccaccac
     1501 ctgaacccgg tcaccggaag accactgccc gctgttcacc ttacgggctg tctgattcag
     1561 gttatttccg atggcggcca gctgacgcag taacggcggt gccagtgtcg gcagttttcc
     1621 ggaacgggca accggctccc ccaggcagac ccgccgcatc cataccgcca gttgtttacc
     1681 ctcacagcgt tcaagtaacc gggcatgttc atcatcagta acccgtattg tgagcatcct
     1741 ctcgcgtttc atcggtatca ttaccccatg aacagaaatc ccccttacac ggaggcatca
     1801 gtgactaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     1861 acgctgctgg agaagctcaa cgaactggac gcagatgaac aggccgatat ttgtgaatcg
     1921 cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg ggatgacggt
     1981 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgtg agcggatgcc
     2041 gggagctgac aagcccgtca gggcgcgtca gcaggtttta gcgggtgtcg gggcgcagcc
     2101 ctgacccagt cacgtagcga tagcggagtg tatactggct taaccatgcg gcatcagtgc
     2161 ggattgtatg aaaagtacgc catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa
     2221 aatgcacgtc caggcgcttt tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     2281 gactgcggcg agcggtactg actcacacaa aaacggtaac acagttatcc acagaatcag
     2341 gggataaggc cggaaagaac atgtgagcaa aagaccagga acaggaagaa ggccacgtag
     2401 caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatag acgctcaagt
     2461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2581 tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc ggtgtaggtc
     2641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2701 tccggtaact atcgtcttga gtccaacccg gtaaggcacg ccttaacgcc actggcagca
     2761 gccactggta accggattag cagagcgatg atggcacaaa cggtgctaca gagttcttga
     2821 agtagtggcc cgactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2881 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgttg
     2941 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3001 aagatccttt aatcttttct actgaaccgc gatccccgtc agtttagaag aggaggatgg
     3061 tgcgatggtc cctccctgaa catcacgtat atagttagcc tgacatccaa caaggaggtt
     3121 tatcgcgaat attcccacaa aaaatctttt cctcataact cgatccttat aaaatgaaaa
     3181 gaatatatgg cgaggtttaa tttatgagct taagatacta cataaaaaat attttatttg
     3241 gcctgtactg cacacttata tatatatacc ttataacaaa aaacagcgaa gggtattatt
     3301 tccttgtgtc agataagatg ctatatgcaa tagtgataag cactattcta tgtccatatt
     3361 caaaatatgc tattgaatac atagctttta acttcataaa gaaagatttt ttcgaaagaa
     3421 gaaaaaacct aaataacgcc cccgtagcaa aattaaacct atttatgcta tataatctac
     3481 tttgtttggt cctagcaatc ccatttggat tgctaggact ttttatatca ataaagaata
     3541 attaaatccc taacacctca tttatagtat taagtttatt cttatcaata taggagcata
     3601 gaattcctgt aacaatagca ataccccaaa tacctaatgt agttccagca agcaagctaa
     3661 aaagtaaagc aacaacataa ctcacccctg catctgcagc tttcttctct aatgtaagaa
     3721 atagtggctt ccagtcacct gtatccttaa tttttaggat atcagatacc acatcatatc
     3781 caaaagaaac atgccccgta atcttcaagt acttggcaaa ctgatctaaa tgtttagccc
     3841 agtcatcata cttcaccgat gccaacgcat taaaaatagc atcacgatcg gctttgctga
     3901 atttcttatt taaaacatcc ttgtattttt caaaagcagc gagagcttca ttcacattgc
     3961 cgattttctt acctttagac ttatcagcaa gttcctgtgc cattttcgaa tatttttcac
     4021 catatttttc agtcagcgtt tgataaaagc taactgttgc atcaacagca tccttaatct
     4081 gtgaattaag gagattattc tgtgcttttt tcaaattttc ttcagcttca tgaacacgag
     4141 cgataccggc attacgatta ttactgacct gagaaatagc cttctggatc tgagttatat
     4201 cagcatttat ccggttaata cgtgtttctg atgctgttac ctgtttttgt ttttcttctc
     4261 taatcttacc ggccccaacc cgtcgtctgg ttgcttcaaa aaaaggacgg ttctgaagcg
     4321 gatcattggc tcttggtgat agttttttga ccagctcatc cagttcttta tatttagcgg
     4381 atgcctgagc cagttcattt cgttttccag cgagcgtttt catttctgca tcacgggcat
     4441 ggatactgga gcttaaacga gaattgagag tcttaatctc tccatccatt ttcaccactt
     4501 cagattgtgc agcagaaagt tttttttggg cgatctcaac agctttagct tcttcactca
     4561 atgcagccag tcgtttctct tcagcttcag ccagtttcaa ctggcgttct gtttcagcct
     4621 tctcccgttc aatctcttta cgtcgttgtt ctgcttcctg aaaagccttt tctgctgctt
     4681 ccgcttcttt acgggctttt tcttctgctt tcgcaaggcg caaacgctct gcttccgcct
     4741 gcatagctgc attattagca tgagcaagct ctgttgctga aggcgtacgt gaggcattgt
     4801 gacgaagagc ctcattcacg atatccttca ggcgctgagt cagcgcatcc ctgtttgcct
     4861 ttgctttcgc ctgtgcttcc gctgcagctt ttgcccgggc agcctgctct gcctgtgttt
     4921 tctttaattg agcagtagac catttagcag ttgcatgaat agctgcagaa ctttcacttt
     4981 tactgcctcc ttttccacct ccgccgccag agccactccc gtcaggagta ccattcaaaa
     5041 gagtaataat tacctgtccc ttatcatcat aaggaacacc atctttatag tacgctaccg
     5101 cggtttccat tataaaatcc tctttgactt ttaaaacaat aagttaaaaa taaatactgt
     5161 acatataacc actggtttta tatacagcat aaaagctacg ccgctgcatt ttccctgtca
     5221 agactgtgga cttccatttt tgtgaaaacg atcaaaaaaa cagtctttca caccacgcgc
     5281 tattctcgcc cgatgccaca aaaaccagca caaacattac cgttctcaga cctcattatg
     5341 ttttactgaa actatgagat gagacatcta tgggacactg tcactttatg gcatggcaca
     5401 cactccggga cgcactaaaa atgacaggca gatcgcgttc acagttttac cgtgatatgc
     5461 gcgcaggcct tgtcagttac cgtaccggca gggacggacg acgggagttt gaaaccagtg
     5521 aactgatccg ggcatacggc gaattaaagc agaatgagac accagaaagg cacagtgagg
     5581 gacatgcaga aaatccacat gatcagcaga cagaacgcat tctccgggaa ctgaatgagc
     5641 tgaaacaatg cctgacgctg atgcttgagg ataaacaggc acaggatatg gatcgcagac
     5701 gccaggaagc agaacgggaa cagctacaaa atgagatagc ccagctcagg caggcactgg
     5761 aactggaaaa gaaacgggga ttctggtcca ggttgttcgg tcgctgaacg ctgtcagaga
     5821 ctgatgataa aatagtcttc ggataataac tcaccgagaa taaatacttt aaggtaggga
     5881 gacactcatg agacgtaccg gaaacaaact ttgtcttatc gccatgataa cagcaacagt
     5941 agctctcaca gcctgtaccc caaagggcag cgtggaacaa catacccggc attacgtata
     6001 tgcttctgat gacggttttg atcccaactt ttccacccaa aaagccgaca caacacgaat
     6061 gatggtgcct ttttttcggc agttctggga tatgggagct aaagataaag cgacaggaaa
     6121 atcacggagt gatgtgcaac aacgcattca gcagtttcac agccaggaat ttttaaactc
     6181 actccggggc acaactcaat ttgcgggtac tgattaccgc agcaaagacc ttaccccgaa
     6241 aaaatccagg ctgctggctg acacgatttc tgcggtttat ctcgatggct acgagggcag
     6301 acagtaagtg gatttaccat aatcccttaa ttgtacgcac cgctaaaacg cgttcagcgc
     6361 gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc
     6421 gcctgataaa ttttaaccgt atgaatacct atgcaaccag agggtacagg ccacattacc
     6481 cccacttaat ccactgaagc tgccattttt catggtttca ccatcccagc gaagggccat
     6541 ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac acatgttcgc
     6601 gctcctgtat cagccgttcc tctcttatct ccagttctcg ctgtata
//
