LOCUS       NZ_OW968131             4073 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P7, complete sequence.
ACCESSION   NZ_OW968131
VERSION     NZ_OW968131.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065713
            Assembly: GCF_905330245.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330245.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,758
            CDSs (with protein)               :: 4,758
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 86 of 261
            Pseudo Genes (incomplete)         :: 185 of 261
            Pseudo Genes (internal stop)      :: 48 of 261
            Pseudo Genes (multiple problems)  :: 53 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968131.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(2896..4073,1..970))
                     /gene="mobQ"
                     /locus_tag="LQ159_RS25520"
     CDS             complement(join(2896..4073,1..970))
                     /gene="mobQ"
                     /locus_tag="LQ159_RS25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            1149..1403
                     /gene="mobC"
                     /locus_tag="LQ159_RS25525"
                     /old_locus_tag="AI2853V1_4930"
     CDS             1149..1403
                     /gene="mobC"
                     /locus_tag="LQ159_RS25525"
                     /old_locus_tag="AI2853V1_4930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1937..2638
                     /locus_tag="LQ159_RS25530"
                     /old_locus_tag="AI2853V1_4931"
     CDS             1937..2638
                     /locus_tag="LQ159_RS25530"
                     /old_locus_tag="AI2853V1_4931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(613..971)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          972..1148
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1938..2638
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1057)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.443"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2900..4071)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.684"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 attttttcag gctggcagca tggctcatcg cttcctttcg ttcggtgttc agctcttccc
       61 agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat ggcattactg gtgagtttcc
      121 atgccgagtt actgtccttc ggcaacgctt cgataatgtc acgtagtgtg tacgaagttg
      181 cgttgtcagg aagcccgata ttcagaaggt cagccacacg gcgggcaggc tctgtcagcc
      241 ctccgttcct gagttcggtg cgggaaatct gtatttccag gttaagcctg ttaatcagac
      301 gattctgttc gccgcgttct gtttcgatac cgcgtgtttc catcgcacag gcagactttc
      361 cgaggtgaat accaggcgtt ttctcaagcc cctgtcgctc gtatgaacga tggtctatct
      421 cttcctgata accggcgttc gccagtgccc tgtttgcatg gtcagcccat gatgcccgcc
      481 acgtctcggc atgtgtccgg tcgttccagt ccctgacctt tccgctgaac ccagcaggtc
      541 cgatagccct cgtggtcagc atgatgtgcg catggggatt tgttttatcc atgtgatgga
      601 acgccacatc agcaatcatg ccctgactga caaagttttc tctgacaaag gcgagaacgg
      661 tttcccgtgc ggcatcctgc ggcagttcgt gggggatggc gagttcaatt tcccgcgcaa
      721 gttgtgagtt cttccggcgt tcggccttct caacggcatt ccagagttcg gcacggttcg
      781 tacaccaggc aggggcggtg gcgggggtca gaatcacagc ctcagccaca ccgcttttgc
      841 gggtgtagtc gtgagtcagt ccggtacgct catcaagcat cagtgaaccg gaacgatagg
      901 cagcggaagc tacggcgctg tagccgctgc ttctggagat aattttcatg ctgagatgat
      961 aaatcgccat ttctgccgta atgccttttt gtagttttct ttcagccccg cagggcgcac
     1021 acaatcgaaa cggagtggag attgtataag tgcgcacttc gtgatttgcc gtgcattatg
     1081 ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc gatttttcat cgcgcacgga
     1141 gttacatcat gagcagaacg ctcgaacaga agattgctga tgctgaggcg agattacagc
     1201 gcctgaaggc gaagagccgg agcctggaca cagcgcagaa ggttgttgtg ggtgctgcat
     1261 tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg tgcctggtta ctccagttcc
     1321 tgaaggcaga ggtgacacga caggctgatg tgacgcgcat actgccgctg attaacgagc
     1381 tggaggcgct gccagaacag tgattgtatc gtccgctcgc cgcagcccca gcgaccgcgc
     1441 gttagcgagc gagtgggcga ggaagcggaa gtgaatcagt cgctaggttg agcacatacg
     1501 cacggttttt cggcaggtga tatgcgctga acccgcatga atactgggtt tccacagggt
     1561 tggagggggt gtattcgaaa cttgcaattc tgctttattg caacgcattg tttttattgg
     1621 ttttttgttt tcagcacccc aagttatcca caggctcgcc gttaagtttt taagatctta
     1681 tttttagatc ttttttttta aaaccttttt aggtcttgta acaccatgat tttaaagatg
     1741 ttctggcaag ataagtggag cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt
     1801 ggagcttttg ggggtgaaag gtggagcttt tgggggtgta atccacagaa aaagggggtt
     1861 gcaaaaaaaa aacttccacc catatgataa gtggcgtaca gtaaaaaaac gccaccaata
     1921 gcaacgagca agcgaaatgt cagatctgat agcttacaaa tcaaacgccc ttatcgaggc
     1981 aagctacaag ctcactttgc aggaacagcg cttcctgctg gtctgtatca gccgcctaaa
     2041 atccggagaa gattcccctt cgccggatga acaaaaaacc atgaccatca cggctgcaga
     2101 gtttcatgat gcctttcctg acatgggacg gcagcacgct gaagctaggc tcaaggaagc
     2161 gatagatcgc ctctgggaca ggtcagtaat catcaagaac gaggaaaaac gagaagagtt
     2221 ccgctgggtg cagtaccggg cgcagtattt caaaggtgag gcgaaagtag atatcacctt
     2281 ctccgatgcc atcatgccgt atctgacaca gctcaagggg cagtttacgc gggtagtcgt
     2341 gaagaacgtc tccagccttt ccagcaccta cagcatccgc atttacgagc tgctccagca
     2401 gttccgcagc accggtgaca gaacgatagc tatcgatgat ttccgaacca tgcttgccgt
     2461 tgaagataaa tatcaacaat tcaaagactt gaataagatg ataatcaagc cagccatcgc
     2521 cgaactgaac gaaaaaagcg atctggttgt aactgttgag acgatcaaaa aggggcgcac
     2581 ggttgtagcc ctgcattttc gcttcaagga agacaaacag atcaagatga cgatctaaaa
     2641 tcgattttaa ggcacctaaa acgctccctg ctgcgttttt tttgtgttag tagtgcgaag
     2701 gtactattga agtgaaaaat tgcgtcaaaa acgctctgat gcagcgaaaa aagacccccc
     2761 aatttttggg ggcggaaaaa caaaactatg cttgcgttat gtgagtgtgc ttcttgcatt
     2821 catccctgtc gatgaacatc gaagaacatc gacgaacata acacgatgtt aaacaaaaca
     2881 aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg tggtgtgtgt
     2941 tcgtgcatct tcctcgcgct gccattcctc ccaggtacga ttaaaaccca gcgagcggaa
     3001 cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg cctcacgggt
     3061 tttcagatga aactgcatca gttctggtgc aggtgatatg tggctgtctg catcgtgatt
     3121 tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagcgcac tgatgaccag
     3181 atgcctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat taagccggat
     3241 aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct ggcgggtttc
     3301 ccggtcgaca gaactcatga ccggcatcag cgtcggtggc agacgtccct gcataaaatc
     3361 caccttcctt gccgctggtg atggcatggc tggctggcgg tcttctccaa gacagaaaga
     3421 ctccggccat gtcttcggct ggcggagatt gcgggtatgt tgcggccatg tctggcaaac
     3481 ctgcgtcagc agttgctcaa aggttgcaga ggaataaaca cgttgcagtg gcggacgttg
     3541 gccgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat caacactctc
     3601 aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtatatt ctccgttccg
     3661 gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatgggag cctcgccata
     3721 ccccgtaacg actgctctca gcacccctga atccataacg gcattgtcat ttttcctgag
     3781 ctgctccgcg cgactacgtt cagtatcttc ctgttccttc cggcgcaatt cctcttcacg
     3841 tttaagcaac tgggctctga gagctgcaag gccggattcc ccccactgct gccactgttt
     3901 ttcaaatgcc atctgactcg cgggaatgtc ctgtttctcc accttccggt aatgctgtcg
     3961 taatttttcc atttgctggc tcagagactg gatttttccc tgctcactac ggtattccgg
     4021 tgccgcccat tgcatcaggg gaatacggga aaaaccggac gaaaacggcg atg
//
