LOCUS       NZ_OW968129            12602 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P5, complete sequence.
ACCESSION   NZ_OW968129
VERSION     NZ_OW968129.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065713
            Assembly: GCF_905330245.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330245.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,758
            CDSs (with protein)               :: 4,758
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 86 of 261
            Pseudo Genes (incomplete)         :: 185 of 261
            Pseudo Genes (internal stop)      :: 48 of 261
            Pseudo Genes (multiple problems)  :: 53 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968129.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(12543..12602,1..258))
                     /locus_tag="LQ159_RS25415"
     CDS             complement(join(12543..12602,1..258))
                     /locus_tag="LQ159_RS25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            1343..1492
                     /locus_tag="LQ159_RS25420"
                     /old_locus_tag="AI2853V1_4911"
     CDS             1343..1492
                     /locus_tag="LQ159_RS25420"
                     /old_locus_tag="AI2853V1_4911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1952..3180
                     /locus_tag="LQ159_RS25425"
                     /old_locus_tag="AI2853V1_REPA000000314"
     CDS             join(1952..2267,2267..3180)
                     /locus_tag="LQ159_RS25425"
                     /old_locus_tag="AI2853V1_REPA000000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            3211..3579
                     /locus_tag="LQ159_RS25430"
                     /old_locus_tag="AI2853V1_4914"
     CDS             3211..3579
                     /locus_tag="LQ159_RS25430"
                     /old_locus_tag="AI2853V1_4914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            3664..4647
                     /locus_tag="LQ159_RS25435"
                     /old_locus_tag="AI2853V1_4915"
     CDS             3664..4647
                     /locus_tag="LQ159_RS25435"
                     /old_locus_tag="AI2853V1_4915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(5106..5803)
                     /locus_tag="LQ159_RS25440"
                     /old_locus_tag="AI2853V1_REPA000000312"
     CDS             complement(join(5106..5554,5554..5803))
                     /locus_tag="LQ159_RS25440"
                     /old_locus_tag="AI2853V1_REPA000000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            5962..7506
                     /locus_tag="LQ159_RS25445"
                     /old_locus_tag="AI2853V1_4917"
     CDS             5962..7506
                     /locus_tag="LQ159_RS25445"
                     /old_locus_tag="AI2853V1_4917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<7599..7863)
                     /locus_tag="LQ159_RS25450"
                     /old_locus_tag="AI2853V1_REPA000000309"
                     /pseudo=""
     CDS             complement(<7599..7863)
                     /locus_tag="LQ159_RS25450"
                     /old_locus_tag="AI2853V1_REPA000000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(8008..8884)
                     /locus_tag="LQ159_RS25455"
                     /pseudo=""
     CDS             complement(8008..8884)
                     /locus_tag="LQ159_RS25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(9083..9367)
                     /gene="nadS"
                     /locus_tag="LQ159_RS25460"
                     /old_locus_tag="AI2853V1_4919"
     CDS             complement(9083..9367)
                     /gene="nadS"
                     /locus_tag="LQ159_RS25460"
                     /old_locus_tag="AI2853V1_4919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(9369..9701)
                     /locus_tag="LQ159_RS25465"
                     /old_locus_tag="AI2853V1_4920"
     CDS             complement(9369..9701)
                     /locus_tag="LQ159_RS25465"
                     /old_locus_tag="AI2853V1_4920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9846..10082)
                     /locus_tag="LQ159_RS25470"
                     /old_locus_tag="AI2853V1_4921"
     CDS             complement(9846..10082)
                     /locus_tag="LQ159_RS25470"
                     /old_locus_tag="AI2853V1_4921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            10427..10795
                     /locus_tag="LQ159_RS25475"
                     /old_locus_tag="AI2853V1_4922"
     CDS             10427..10795
                     /locus_tag="LQ159_RS25475"
                     /old_locus_tag="AI2853V1_4922"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(11225..11359)
                     /locus_tag="LQ159_RS25480"
     CDS             complement(11225..11359)
                     /locus_tag="LQ159_RS25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     Typing          1135..1308
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(660..1168)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(11252..11617)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
ORIGIN
        1 ttttttgcgg gcttcgcctt ttgccatgcc atttcctatc ccaaaatcac ccaggaaacc
       61 taatacggtg cgcccgtcaa attcgccggt ttcctcaatt tcttgttgta cactgcgcgt
      121 tttggcgatt tccagagcta ccgctttgca atccattgcg gaagattctg gaccggtgac
      181 gttcacggcc tgtggatatt gctttgttgc gcatcctgcc atgatcagcg ctgccgatag
      241 gatgaatatt tttttcaaaa ttatccttat ttatttaggc atagttaaga gatacccatt
      301 ttatcggcag gaaatgaggg ttttttagtt gaattttggg aggggcaagg ttccatattg
      361 gtcgaggatt ctggagctga ccggcaacgg ccagcgcggc tatgggccat gcggtggttg
      421 agcatagaaa ggatcttctt gagttccttt ttttctacgc gtaatctctt gctctgaaaa
      481 cgaaaaaacc acctggggag gtggtttttc gaaggttaag tcagttgggg aactgcttaa
      541 cctggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
      601 gttaggccac cacttcaaga actctatagc accacctaca ttaactcgct ctgctaatcc
      661 tgttaccagt ggctgctgcc agtggcgctt tgtcgtgtct ttccgggttg gactcaagac
      721 gatagttacc ggataaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca
      781 gcttggagcg aactgccttc ccggaactga gtgtcaggcg tggaatgaga caaacgcggc
      841 cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag
      901 ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
      961 gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc
     1021 gcggccctat cgcttccctg ttaagtattt ccctggcatc ttccaggaca tctccgcccc
     1081 gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     1141 aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgcc gccttttttg
     1201 tcctgccaca tgaagcactt cactgactcc ctcatcagtg ccaacatagt aagccagtat
     1261 acactccgct agcgctacgt gactggttca gggctgcgcc ccgaaaccct ctaacaacct
     1321 ctcagctact ggatatcacc ccatgccatc tgaacatgat gaacaactgg attcgactga
     1381 tacgcagctt cccggcgaat atgacctttt tgatttaatg gcgcaggtta ctgaagagaa
     1441 cctgcatgcc ctggtggatt ttgggccgcc tgtcggccgg gaagcgttat agctttttcg
     1501 cttacccgcg tacgtggtaa tacgccggta ttacatctta tttctttatt ttcatgtaat
     1561 tacaggtggt tgtaatacgt tgcgtattac ataaataaac ggggacttca gtttttatct
     1621 tttaattcat gcaattgcga tgaaaatcgc ggttgcgtat ggtgtatgac catttaaggg
     1681 ataatgtaac ctgactttta aagaatgaat ctatctttgg cagcgcatga gatcgataaa
     1741 aagcgttcca catcaggcgg ttcatgtatc catgaaccgg aaaggacaaa aataagacaa
     1801 ttgtaagaca ttttttgcgt tttactctta ttttggacta ttatgagaca aatgtaagac
     1861 tagactggcc ccctgaatct ccagacaacc agtatcactt aaataagtga tagtcttaat
     1921 actagttttt agactagtca ttggagaaca gatgattgat gtcttagggc cggagaaacg
     1981 cagacggcgt accacacagg aaaagatcgc aattgttcag cagagctttg aaccggggat
     2041 gacggtctcc ctcgttgccc ggcaacatgg tgtagcagcc agccagttat ttctctggcg
     2101 taagcaatac caggaaggaa gtcttactgc tgtcgccgcc ggagaacagg ttgttcctgc
     2161 ctctgaactt gctgccgcca tgaagcagat taaagaactc cagcgcctgc tcggcaagaa
     2221 aacgatggaa aatgaactcc tcaaagaagc cgttgaatat ggacgggcaa aaaagtggat
     2281 agcgcacgcg cccttattgc ccggggatgg ggagtaagct tagtcagccg ttgtctccgg
     2341 gtgtcgcgtg cgcagttgca cgtcattctc agacgaaccg atgactggat ggatggccgc
     2401 cgcagtcgtc acactgatga tacggatgtg cttctccgta tacaccatgt tatcggagag
     2461 ctgcccacgt atggttatcg tcgggtatgg gcgctgcttc gcagacaggc agaacttgat
     2521 ggtatgcctg cgatcaatgc caaacgtgtt taccggatca tgcgccagaa tgcgctgttg
     2581 cttgagcgaa aacctgctgt accgccatcg aaacgggcac atacaggcag agtggccgtg
     2641 aaagaaagca atcagcgatg gtgctctgac gggttcgagt tctgctgtga taacggagag
     2701 agactgcgtg tcacgttcgc gctggactgc tgtgatcgtg aggcactgca ctgggcggtc
     2761 actaccggcg gcttcaacag tgaaacagta caggacgtca tgctgggagc ggtggaacgc
     2821 cgcttcggca acgatcttcc gtcgtctcca gtggagtggc tgacggataa tggttcatgc
     2881 taccgggcta atgaaacacg ccagttcgcc cggatgttgg gacttgaacc gaagaacacg
     2941 gcggtgcgga gtccggagag taacggaata gcagagagct tcgtgaaaac gataaagcgt
     3001 gactacatca gtatcatgcc caaaccagac gggttaacgg cagcaaagaa ccttgcagag
     3061 gcgttcgagc attataacga atggcatccg catagtgcgc tgggttatcg ctcgccacgg
     3121 gaatatctgc ggcagcgggc ttgtaatggg ttaagtgata acagatgtct ggaaatatag
     3181 gggcaaatcc aaagacatta tgggcggtat atggcgaatt ttaaccctgg tccaagaaca
     3241 gccattgcag ctgtcaggga ttccaagaac gatatagaaa cctggaagaa aaatggtatt
     3301 cgtagggaag agattgtgac tgtgctgaat gaacgttacg gcctgaatat ctcactggtt
     3361 gcgtttgata aggctcttta cagattgaga aaaagtacag atgaaccgtc tcttgctgat
     3421 gtggattctg tgtctgctgc agaaacgaat ttctctgaag ccagtgggcc tgacaatggt
     3481 ttttttctgt ccgggaatga cttcatgcga gcagaagaag aatctcaaag agtggttgag
     3541 tcattcagag atagccttag caggaagaaa ggattctgat ttattcaaca aagccgttga
     3601 taaatcgcat tccataaata acaacttgat gttagtataa ctaaatattg tttggagatt
     3661 actgtgaaaa aaaaattttc gataatagta cctgtatata acgttgaaaa atacttaagg
     3721 gaatgtattc aatcagtatt agatcaggat tttaacgact atgagataat ctgtgtaaat
     3781 gatggttcaa cagatggcag tctagatatc atactgtcat ttgatgacca aagaatcaaa
     3841 gtaataaatc aggaaaataa aggcctgtcg gtggcaagaa atgtcgccat tgaatcatca
     3901 actggtgaat atatatggtt tttagatagt gatgataaaa tatcacatgg aagtctaaag
     3961 ttaatagaca agataaccaa tgaaaaaaat tatgggatta ttttctttaa tacacatgtt
     4021 ttttttgaca ctccagaact gaaaaaaaaa ggctggcatg attataagcg ttcaacattt
     4081 ggtgaaatgg atgcattaac tttctttgaa aaagaaatga cgaacaaaaa actaatagtg
     4141 tcagcatgct gctatgtttt tagaagggaa ttattaaatg gcaatgcatt cattccgcat
     4201 atatatcatg aggataatca ctttatagca accctgattt ttgacaatta ttcaactata
     4261 tgcctctcgt tggatgaggc gttatatctt aggagggtaa gaaatgattc cataatgacg
     4321 tctaaaaaat caatgaggca catcattgga tattcaacat gcatcgatga gcttacgaag
     4381 tataaaaaaa caaaaggcat acaaaacaag agctttgata gatttgtgaa tggaatggtt
     4441 ttgtcgttat tctctatagc ggaaacaata aaaacaaagg aagcaatgaa ctatgtggcg
     4501 agatataaaa aacaccccga cctatcattt aaagatagag cattaattaa ttacccatta
     4561 attaaaaaga cttatttaaa actaaaaaac gggaaggtgt ttaattacct aaggaatggc
     4621 attattaata aaaaaagcaa cccttaataa aagcaaagcg ataattaata aaggtttttc
     4681 tttgttgaat aaataagatt tcggataggt atccccgtga cgggttgggt tttcaggcga
     4741 tacgcacgct ttccggggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     4801 atcggggtca gtttggagat cggaaatttt tgtacgttaa gcgcattatt taaccaatct
     4861 tggttcgtaa ggggttgatt ttgcgtcagt ggctatcgct tttaagttgt cgctcttgca
     4921 gtatcgcatt taggttatcc gattaaggtc aaacctctga aaatgccgta tagcgcggga
     4981 gtacacccta ttgctatagg taagtctgtt caaaaaacag gcttaccgta caataattct
     5041 ctatatccaa actaacccca gatcacgcat cttcccgaca acgcagaccg ggtaatgact
     5101 ccaacttact gatagtgttt tatgttcaga taatgcccga tgaccttgtc atgcagctcc
     5161 accgattttg agaacgacag tgacttccgt cccagccttg ccagatgttg tctcagattc
     5221 agattatgtc gctcaatgcg ctgcgtatat cgcttgctga taacgtgcag ctttcccttc
     5281 aggcgtgatt catacagcgg ccagccatcc gtcatccata ccacgacctc aaaggccgac
     5341 agcaggctca gaagacgctc cagtgtggcc agagtgcgtt caccgaagac gtgcgccaca
     5401 accgtcctcc gtatcctgtc atacgcgtaa aacagccagc gctgacgtga tttagcaccg
     5461 acgtagcccc aatgttcgtc catttcagcg cagacaatca catcacttcc cggttgtatg
     5521 cgcgaggtta ccgactgcgg cctgagtttt ttaagtgacg taaaaccgtg ttgaggccaa
     5581 cgcccataat gcgtgcactg gcgcgacatc cgacgccatt catggccata tcaatgattt
     5641 tctggtgcgt accgggctga gaggcggtgt aagtgaactg tagttgccat gttttacggc
     5701 aatgagagca gagatagcgc tgatgtccgg cagtgctttt gccgttacgc accacgcctt
     5761 cagtagcgga gcaggaagga catctgatgg aaatggaagc cacgcaagca ccttaaaatc
     5821 accatcatac actaaatcag taagttggca gcattaccca ctattggtgg agatgatcat
     5881 aatgccttat aatcatacgg gatacgaact tataaagtcc taaagtttag ctaaatcgga
     5941 atctacaacg tagatgatag cttgaagaat tatgatgtct tttatggtgg tatctttaaa
     6001 accatcacgg attataaatt attgaaaatt caaaaggata ttaaaaacaa tatgactgca
     6061 ctagtcagta gttttaaatg ggttggcatt ggacaacttg taaaaatagc aacacaattg
     6121 gtttcgttat tctacttgac gcacttaatc cccccatcga gctatggact attagcaatg
     6181 tcatctgtgg ttatgaattt agcaagcata ttcaatgaca tgggaactgg ggcggcagta
     6241 attcagcgac aagaggcgtc gatcggtttt tataattaca tatacaggat aaacattttt
     6301 actgggttgt ttgttatgct gcttgtaata ataacatccc cattaattgt cgtgtacttt
     6361 aacacacccg agctataccc ggttctattt ctattggctt ttagctttcc aatttccagc
     6421 ttaacaataa ctcacaaagc aaaattagaa aaagaaatga actttaaagt catcattgca
     6481 attgaaattt tctctagcct tacaggaatg gtgctggcaa tagttttagc taatttaggc
     6541 tttggtgttt atagtttgat ttttcaaaca ctgacaacaa tatcaatatc cacaatatgt
     6601 tttttgaagg tttccgggtt gagtttatca ttgagaatta aaaatatcag ccatagtgac
     6661 aagtcaggag ttatagggtt tagtggttat ttattgtctt ttaatttaat aaattatttt
     6721 tccaggaatc ttgattctat tttaataggc cgttttttct catccgcaat acttggggtc
     6781 tattcgattg cctaccgcat tatgttattt ccgttgcaga gcctgacgct tgttttttct
     6841 agagttttct tgccacacct tagcaaaaac ataaataaca ctaataacac taataaacaa
     6901 gattacttaa atgcattaaa acttatacta tcattaagcg cgccactgat gctaggttta
     6961 tctgctacat ctttttcttt tacgaggatt ttctttgatc ctaagtggca cctacttgga
     7021 gacttattgc attggttggc acctacagcg attatccaat cagccttgag tacaacaggt
     7081 tctgttttta tggcctacgg aaaaacaagg tggttgtttt tcttaggttg cattggcgct
     7141 tttttgatgg ggttgtcttt cattataggt gttcatttcg atataaaaac attggtctac
     7201 ctttattttt ttgcaaactt aattaattca ctcccggcat ttattttact taagaatatc
     7261 ttgaagttta gttacattga attggtaaac gtgtttttta gaactatctc cccagctttt
     7321 ttgatgttct tacttattaa agtatggcag catttttcct ttttcaaaaa tgccagcatt
     7381 gatttttctt ttaatgtcgc attgggtatt tttagttata ttgtttttta taccttgttt
     7441 aatttcaatg aggttgttag gacaggagta attcataagt taagatgcat gaccaagtta
     7501 aagtgattca tttattaaat tcattaaatg aattggtaat gactccaact tactgcttgt
     7561 gttttatgtt cagataatgc ccgataccga cttcggccta atttttttaa gtgtcgtaaa
     7621 atcgtgttga ggacagtgcc cataatgcgg gatgtggccc ggcatccaac gccattcatg
     7681 gccatatcaa tgattttctg gtgtgtaccg ggctcagagg cggtgtaagt gaactgtagt
     7741 tgcttgctat gttttacgac agtgagagca gagatagcgt tgatgtccgg tagtgctttt
     7801 accgttacgc accacgcctt cagtagctga acaggaggga taactgatgg aaatggaagc
     7861 cacgggagca cctcaaaaac accattagac actaaaccag caagttggca tcatcacaaa
     7921 cagttgttac tgctggaaaa aaaacactat caacacttaa gtaatggtga ttcaataatt
     7981 aagattcagt atgtaaaata ctgaatctta attataaatt ttcctttttc taaaaaatgt
     8041 agcgcgacta atacctaaag caacccaagg ctcccctaga gacagcctac ctcctcctga
     8101 tgatttttca ccaccaactt ttcctctttt ggcttgtata agtgaaaaat tttgcttatt
     8161 aaatttcaaa aatgtccact tgcagatact tttcgctatt cccgttactt cattctcatt
     8221 caaaggcact gcaaattgca gattgtaagc gcaggcgcgt tcatggcagg cttgtagcca
     8281 ctgttcgtac tcaggccagc cctgacggat agcgcgatat gcccactttc tggttttatc
     8341 aaacagtatg cagttacgac ctaagccata atcggcgacg atttttttat cgttcgcagc
     8401 gttcaggtcg agtgattcgg ctaaccagtc cagcgtgtag agttcaggct gccatacggg
     8461 caattttcca gtgactgtgg ttcggattct tgcaaattag ccccgaatac cccgtgtcag
     8521 cgcccaattt tttacgtagc gcgctctcta cagcagcggc gtaacgtaac ggtttcattt
     8581 ttccgtctgg cgcggtgcgt atcgaggttt tcagtgcgta cagcaggtgc gcgtgtccgt
     8641 tttcggggtt ggtgatggtc agtgtcggcg cgggggcgtt gcggtcgctc cagtcaatgg
     8701 ctgcgccgat gcggtcaacg tcaaatccca gccagaacat ggcgtgaggc tgattaaact
     8761 ggatgtattt agccagaata gcgcgctctt tccctgcgat gcggacacca aagtgtaaat
     8821 catccgagaa gtacggttta tgcggcaagc ggtcgttgaa aagttcgagt gctgcgttac
     8881 gcaatgtgcc ctgaacttat ggcgggctgt tgtctggcac ggttcatgtg ctaatatcct
     8941 tcttaggttt tatacctagc cctgatttat catgtctctt ccgccaagtt gaaatcatga
     9001 ttatcggggc tttgtttttt ctgttgccgt taaagttaca cgcgaacggc ctcgcctgtc
     9061 gaatttcttc tgtcacatca gcttaatgcg ccgccagttc atcaaaaaac tttgggttag
     9121 cctgaatggt ggccagcact tttgctgcca gtccggttgg gttacggcgt ccggtttccc
     9181 agcttttgat ggtatcgagg ctggttccca gcactttcgc catttcgctc tgagtaatat
     9241 tcaggttttc tctgatagcc ctgacgtctg ccagctcgta gcgggtcaca cgggcggggg
     9301 ctttggttcc gtttttgatt tcaacggcct cttccagaga ggctttcagc tcgtcaaaaa
     9361 aactcatctt acacctcgtc tttcagtcgt tgggtcagct tcttcagttc tgctttctct
     9421 gcgtctgtga tgctgtcttt ggtgctttta gggtaggcca tcaccagata aatcacctct
     9481 gcggtggcaa ggaagtaaat cacccgcgca ctgccgctct ttccctgatt ccccgtcgcc
     9541 atcctgatct ttcgcagacc gcccgttttc tggatgaggt cacctttgtc cggtgactcg
     9601 ataagctctt tctgaagctc tttgatctca tcatccgtgg cgatttgctt tatctgacgg
     9661 gtaaacatcg ttgtctcgat aaactcaatc gcgtgactca cggctgtctc ctgtatattt
     9721 tgatgtacaa tgtacaccaa gaggcgtgtt ctgaaaagcc gcgttctgtg ccatcagacg
     9781 ttacatggac tgacctcgat agcgggttgc ttgcttctcc tgctgtaact gccgttcctg
     9841 ctgctttacg agcgtgttaa tcggctcaga tcgctgctca agcgttcgac ctgttcgtgc
     9901 aaggcgttca cctgattgct caagttcaga cataccctgt cgttgcgctc cagtgcctgt
     9961 gaggtagtct cgaacatgcg ttgcaagctg ctgtaactgt cctgccacgc ctgcttctgc
    10021 tgttcgtagc ttgtttgtaa gctttctaag gcgctcagaa gctgcctttc catctcggtc
    10081 atggtttttc ctgtcctcag tgataaaaac gtgctgtggc gtttctgtgg tgtctgacgg
    10141 ggttatttcg cggtgtactt catcacggtg tttccggctg tcgatgtgta tgtctcggtg
    10201 ccatgcgctt tcttcggcag taccaccaga taggtgttct tctgcttgtc gtccttgtaa
    10261 atcgctaccc ctgcgccgct ctggggtccg cttggaaaaa ggaaattatc ctacgctaag
    10321 cgcatttttt gcacagatgg ctaccgactg aacgtaacgt gtgagtcatg atcagaaatg
    10381 tgttaactct aggaattgac acgacttact gtttgatggg gaaatcatgt ttagctatct
    10441 caaggctatg tatcatcaat caaaaattca ggctgaactc aaggcacaga tacatgaaca
    10501 aactacggtt aatgctatct gtcatcaccc tgaatcaatc gagatcattg cggtttgttc
    10561 gactgatgcg tattaccgaa aacgtaagga cgctgctttt ctgacaacct gttctgtgct
    10621 catgcgtact ttaaaagatg aatccgtacc gatggtacta cggaaaacag catggcgatt
    10681 attgaacgaa agataccaac gaattaagtt aaatcaggct tataggatag aaaatttctt
    10741 gcttgttgcc gattttgaat atgctctcga agaacatgat gaactagcag agtagtacta
    10801 gtgaggcgat gaataccgac agttaacgtg catgtgactt aaacgtccga ttacgtacag
    10861 catgtagaca acatgtccga aagtaagcaa aaccattaac ggacagccgg atcatttcgt
    10921 acatccctta cggtcagggg ttcgcgctca atggaacgaa atcacccgtt agcttaaaac
    10981 gcttaacgta ggataatttc ctttttccaa gcggacccgc aatacagcaa ggaacttctt
    11041 gagatccttt ctgttgcgtc gtaatctctt gctctgtaaa cgaaaaaacc accctggcag
    11101 gtggtttttc gaaggttagc taatcctggc agattttcta accgcggtaa cagtcttgtg
    11161 cgagacatgt caccaaatct gtcctttcag tgtagccgca ctcaggccat cacttcaaga
    11221 actcttactg catctctcgc acatcctgtt taccaatggc cgctgccagt ggcgtttttg
    11281 cgtgtctttc cggattggac tcaagacgat agttaccgga caagacgcag cggtcggact
    11341 gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg
    11401 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag
    11461 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
    11521 cctgtcgggt ttcgccacca ctgatttgag cgtcagattc tgtgatgctt gtcagggggg
    11581 cggagcctat ggaaaaccgg cagcgccgcg gcctttcctc cctcgcttta tctcgtcttc
    11641 atcgccatat gcctttgtga atattctccg tccgctcgcc gcagccgaac gaccgagcgt
    11701 agcgagtcag tgagcgagga agcggaatat cttcggcctc acgtccggca ccttacactc
    11761 cgtaactgca ttttttcata ggatgcagga tagggctaac gccgcaaagt gacgtcacct
    11821 gacgttcctg gattacaaag gttaaagcag cggacaggaa atgttttgtg cccacagcta
    11881 tgctgggtta ttcacaaaac agcgggacaa aatgtgtttt tggagtaccg ccggcccgct
    11941 aaagactcct ggttagctcc cgcatgtttg ctatcttttt gctaccattt tgctactttt
    12001 ttgctatctc cagagtcttc tggtctttcg gttgctatcc ttttgctact tttttgctat
    12061 caaaatgcta cctctccctt cttgcaataa atgaccaagg cacgttagcg atgtatacat
    12121 cgccgcgccg caagggtgcc ccttccgaat cccgttcatc actgtgtgct gattaatcca
    12181 ggttaaatcc gaccccctta gcaataaatc ctgccatgcc tgacggcctg gccaacgcct
    12241 cacggtcgca cagtaaactg cgcacccttt caccgttatc ggtggggttc gtggtggctt
    12301 tcagggcgct cgccggattt tagccgctaa aatgacgtcc atgcgttcgt gtgcatagcc
    12361 atgcagacgc gaagaaatca tcaggcgcgt tttttcacga tatggacagg gaaagatgcc
    12421 ggaaataggc aggagggcgc agaaatgccc tgagggcgtc tgagatggtg cacgcaacgg
    12481 atgttatggt aaattcagct gattggcagg tacaaaaaaa gccccgaggg cttttttgta
    12541 ccttattgat ctgcacactt tgcagccttc agggcttcca gctgatttag ccggttagct
    12601 gc
//
