LOCUS       NZ_OW968128            27551 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P4, complete sequence.
ACCESSION   NZ_OW968128
VERSION     NZ_OW968128.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065713
            Assembly: GCF_905330245.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330245.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:03:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,019
            Genes (coding)                    :: 4,758
            CDSs (with protein)               :: 4,758
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 86 of 261
            Pseudo Genes (incomplete)         :: 185 of 261
            Pseudo Genes (internal stop)      :: 48 of 261
            Pseudo Genes (multiple problems)  :: 53 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968128.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(26655..27551,1..81))
                     /locus_tag="LQ159_RS25220"
     CDS             complement(join(26655..27551,1..81))
                     /locus_tag="LQ159_RS25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_024245156.1"
                     /translation="MRYLTELICDLYRLTDHHKLTTATFKNLADIKLFKPTADADTILQ
                     LENIFSEYSLRYIDKDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVING
                     SWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRPPINVKYYSWFDRYVAS
                     NSGGSHHAAMVVYQSVRDGLEYKREAVIKQLSIDPGTVEILDKNYYSFIFQIKTLNKET
                     RIYTSEYEFTDALKDFGQNRHTILKPVNYVSNIKLVFIPKDALKTNSETFRNWFHSAIS
                     CGKIISLPDYLKNPAHYHTLHYSHEVDSITLGDPSRKFKLKGDS"
                     /name="DUF6685 family protein"
     gene            complement(106..387)
                     /locus_tag="LQ159_RS25225"
                     /old_locus_tag="AI2853V1_4871"
     CDS             complement(106..387)
                     /locus_tag="LQ159_RS25225"
                     /old_locus_tag="AI2853V1_4871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     gene            complement(485..1147)
                     /locus_tag="LQ159_RS25230"
                     /old_locus_tag="AI2853V1_4872"
     CDS             complement(485..1147)
                     /locus_tag="LQ159_RS25230"
                     /old_locus_tag="AI2853V1_4872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            1528..2172
                     /locus_tag="LQ159_RS25235"
                     /old_locus_tag="AI2853V1_4873"
     CDS             1528..2172
                     /locus_tag="LQ159_RS25235"
                     /old_locus_tag="AI2853V1_4873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203396.1"
                     /translation="MTSNVPTSFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGTRLERPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKAKKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISNALKRKTGQKSSK"
                     /name="recombinase family protein"
     gene            complement(2327..2410)
                     /gene="dinQ"
                     /locus_tag="LQ159_RS25240"
     CDS             complement(2327..2410)
                     /gene="dinQ"
                     /locus_tag="LQ159_RS25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="WP_000565612.1"
                     /translation="MIDKAIIVLGALISLLELIRYLLQLMG"
                     /name="dinQ"
     gene            2391..2825
                     /locus_tag="LQ159_RS25245"
                     /old_locus_tag="AI2853V1_4874"
     CDS             2391..2825
                     /locus_tag="LQ159_RS25245"
                     /old_locus_tag="AI2853V1_4874"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053273090.1"
                     /translation="MIALSIIRLLHYMEQTPDTYSYTACHIGCSSVLVEDSSTPARATN
                     SGLENTKKPDYQSGFFVPVVRLFFRFFPSFGGFFFHFLLRPILLFALANCVMALLTFKS
                     IVRNNTVQNIYIRACTEVSLTINEHLDQNLLAEIIGKIRK"
                     /name="hypothetical protein"
     gene            complement(2839..>3177)
                     /locus_tag="LQ159_RS25250"
                     /pseudo=""
     CDS             complement(2839..>3177)
                     /locus_tag="LQ159_RS25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241261.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3193..3890)
                     /locus_tag="LQ159_RS25255"
                     /old_locus_tag="AI2853V1_REPA000000304"
     CDS             complement(join(3193..3641,3641..3890))
                     /locus_tag="LQ159_RS25255"
                     /old_locus_tag="AI2853V1_REPA000000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            <3946..4197
                     /gene="tnpA"
                     /locus_tag="LQ159_RS25260"
                     /pseudo=""
     CDS             <3946..4197
                     /gene="tnpA"
                     /locus_tag="LQ159_RS25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525033.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /name="tnpA"
     gene            complement(4389..4583)
                     /locus_tag="LQ159_RS25265"
                     /old_locus_tag="AI2853V1_4877"
     CDS             complement(4389..4583)
                     /locus_tag="LQ159_RS25265"
                     /old_locus_tag="AI2853V1_4877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053273080.1"
                     /translation="MEKIGNDIPHLIDQAIDFMASTQAFREYLKKLPPRNTIPSDIPEE
                     NVPLYLQRLEYYRQLYRPK"
                     /name="hypothetical protein"
     gene            complement(4885..>5124)
                     /locus_tag="LQ159_RS25270"
                     /old_locus_tag="AI2853V1_REPA000000302"
                     /pseudo=""
     CDS             complement(4885..>5124)
                     /locus_tag="LQ159_RS25270"
                     /old_locus_tag="AI2853V1_REPA000000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            5190..5894
                     /locus_tag="LQ159_RS25275"
                     /old_locus_tag="AI2853V1_4879"
     CDS             5190..5894
                     /locus_tag="LQ159_RS25275"
                     /old_locus_tag="AI2853V1_4879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<5947..6549)
                     /gene="intI1"
                     /locus_tag="LQ159_RS25280"
                     /old_locus_tag="AI2853V1_4880"
                     /pseudo=""
     CDS             complement(<5947..6549)
                     /gene="intI1"
                     /locus_tag="LQ159_RS25280"
                     /old_locus_tag="AI2853V1_4880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /name="intI1"
     gene            6695..7533
                     /locus_tag="LQ159_RS25285"
                     /old_locus_tag="AI2853V1_4881"
                     /pseudo=""
     CDS             6695..7533
                     /locus_tag="LQ159_RS25285"
                     /old_locus_tag="AI2853V1_4881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            7656..7988
                     /gene="qac"
                     /locus_tag="LQ159_RS25290"
                     /old_locus_tag="AI2853V1_4882"
     CDS             7656..7988
                     /gene="qac"
                     /locus_tag="LQ159_RS25290"
                     /old_locus_tag="AI2853V1_4882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /protein_id="WP_000800531.1"
                     /translation="MKNWLFLAIAIFGEVVATSALKSSHGFTKLVPSVVVVAGYGLAFY
                     FLSLALKSIPVGIAYAVWAGLGIVLVAAIAWIFHGQKLDLWAFVGMGLIVSGVAVLNLL
                     SKVSAH"
                     /name="qac"
     gene            complement(8235..8867)
                     /locus_tag="LQ159_RS25295"
                     /old_locus_tag="AI2853V1_4883"
     CDS             complement(8235..8867)
                     /locus_tag="LQ159_RS25295"
                     /old_locus_tag="AI2853V1_4883"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="WP_020802982.1"
                     /translation="MKVPQVRGMEFYPSCIEKGMRSERALKLAIAEMYVKGVSTRRVSD
                     IVEILCGTEVSSSQVSRLAKELDEEITSWKAQPVGQIQYLVLDATYESVRVGSHVVKQA
                     LLVAIGVDYSGNRHILDAEVANSEAEVNWRSFLEGLVRRGMHGLRMITSDDHSGLRAAI
                     DAVFPGILWQRCQFHLQQNAHSYVTKKDEIPLIAADIRKVFNRNMSR"
                     /name="IS256 family transposase"
     gene            8890..9165
                     /locus_tag="LQ159_RS25300"
     CDS             8890..9165
                     /locus_tag="LQ159_RS25300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109023896.1"
                     /translation="MNIGLFGFTNKHYYLATKTQEKYTKPFGSDGSLSPLDYSVGIGTG
                     LELGHFQFMFGYDYPLSNSSKDPDLSQVRQHNFRASIAYSFRKLKK"
                     /name="hypothetical protein"
     gene            complement(9168..9959)
                     /gene="sul3"
                     /locus_tag="LQ159_RS25305"
                     /old_locus_tag="AI2853V1_4884"
     ARG             complement(9172..9959)
                     /name="sul3"
                     /gene="sul3"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul3"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(10428..10673)
                     /locus_tag="LQ159_RS25310"
                     /old_locus_tag="AI2853V1_4886"
     CDS             complement(10428..10673)
                     /locus_tag="LQ159_RS25310"
                     /old_locus_tag="AI2853V1_4886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="WP_001354008.1"
                     /translation="MKIEIMEYNPDWTKNFEEEKIKLLHFFGSHAVAIEHIGSTAIPNQ
                     RAKPVIDIFIGVSPFAELPFISAFLMQRSITTLRQI"
                     /name="GrpB family protein"
     gene            complement(10711..11574)
                     /locus_tag="LQ159_RS25315"
                     /old_locus_tag="AI2853V1_4887"
     CDS             complement(10711..11574)
                     /locus_tag="LQ159_RS25315"
                     /old_locus_tag="AI2853V1_4887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_048207714.1"
                     /translation="MIPNSENKRVWFITGASKGLGYAFTCAALKAGDKVVAVARTIDNL
                     AKLEETYQESLLPLNLDVTDREAVFSTVETAVKHFGRLDIVVNNAGIMIMGMIEELNES
                     DARKLMDTNFFGALWVCQAVMPYLRSQRSGHIIQITSIGAIISGPMSGIYSASKFALEG
                     MSEALAKEAEHFGVKLTMVEPGGYWTDLYTSMSYSNPLDSYGTLRDELAKQYSEDSVDS
                     DPSLAAEALMKLVASNNPPLRLILGSMVYDLAMDTLKARMATWEEWEAVSRASEKAIPA
                     PERYGV"
                     /name="SDR family oxidoreductase"
     gene            complement(11720..>11977)
                     /locus_tag="LQ159_RS25320"
                     /old_locus_tag="AI2853V1_4889"
                     /pseudo=""
     CDS             complement(11720..>11977)
                     /locus_tag="LQ159_RS25320"
                     /old_locus_tag="AI2853V1_4889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492336.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(12032..12736)
                     /locus_tag="LQ159_RS25325"
                     /old_locus_tag="AI2853V1_4890"
     CDS             complement(12032..12736)
                     /locus_tag="LQ159_RS25325"
                     /old_locus_tag="AI2853V1_4890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            12850..13626
                     /gene="aac(3)-IVa"
                     /locus_tag="LQ159_RS25330"
                     /old_locus_tag="AI2853V1_4891"
     ARG             12851..13623
                     /name="aac(3)-IVa"
                     /gene="aac(3)-IVa"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IVa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            13855..14880
                     /gene="aph(4)-Ia"
                     /locus_tag="LQ159_RS25335"
                     /old_locus_tag="AI2853V1_4893"
     ARG             13856..14877
                     /name="aph(4)-Ia"
                     /gene="aph(4)-Ia"
                     /gene_name="aminoglycoside O-phosphotransferase APH(4)-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            14955..>15191
                     /locus_tag="LQ159_RS25340"
                     /pseudo=""
     CDS             14955..>15191
                     /locus_tag="LQ159_RS25340"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /GO_function="GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /name="AAC(3) family N-acetyltransferase"
     gene            complement(15302..16054)
                     /locus_tag="LQ159_RS25345"
                     /old_locus_tag="AI2853V1_4894"
     CDS             complement(15302..16054)
                     /locus_tag="LQ159_RS25345"
                     /old_locus_tag="AI2853V1_4894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654541.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_000602738.1"
                     /translation="MILNTIAEKLKRQSKDDFKGRHFEAWLIVQAVAWYLRYPLSYRDL
                     EEMFRERGFEVDHSTINRWVLAYAPVIEKRLRQFRRPHCGSVRIDETYVKIRGKWRYLY
                     RAIDKHGNPVDFLLTAKRDLDAAKRFFRKMLKDEPLLSPNRIGTDGANTFPSTIKTSVD
                     DGLLHPDPVHYVTKHLQQGIESDHFRVKKNMPKIGGFQSFNTARRTIAGFEAMLWLRKG
                     FGFSGGWTVNDQNDLLARLFGLQKVNKA"
                     /name="IS6 family transposase"
     gene            <16116..17639
                     /locus_tag="LQ159_RS25350"
                     /old_locus_tag="AI2853V1_REPA000000308"
                     /pseudo=""
     CDS             <16116..17639
                     /locus_tag="LQ159_RS25350"
                     /old_locus_tag="AI2853V1_REPA000000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612398.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            <17672..18340
                     /locus_tag="LQ159_RS25355"
                     /old_locus_tag="AI2853V1_REPA000000307"
                     /pseudo=""
     CDS             <17672..18340
                     /locus_tag="LQ159_RS25355"
                     /old_locus_tag="AI2853V1_REPA000000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            18618..19244
                     /locus_tag="LQ159_RS25360"
                     /old_locus_tag="AI2853V1_4897"
     CDS             18618..19244
                     /locus_tag="LQ159_RS25360"
                     /old_locus_tag="AI2853V1_4897"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="WP_230163700.1"
                     /translation="MCALRRAGIVERIDADQWRIPDDLVSRAAAHDAGRDSQASVRVLS
                     PVDLNKQIGSDGATWLDRRLIHGETADLAPTGFGQQVREAMDQRREHHIEQGDATRSRD
                     SRVFYRRNLLAILREREVAGVGSDMALSKGLPFRAATDGESVSGKFTGTVHLSSGKFAV
                     VEKSHEFTLVPWRPIIDRQLGREVMGIVQGGSVSWQLGRQRGLER"
                     /name="DUF3363 domain-containing protein"
     gene            19461..20675
                     /gene="floR"
                     /locus_tag="LQ159_RS25365"
                     /old_locus_tag="AI2853V1_4898"
     ARG             19462..20672
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="99.5"
                     /coverage="100.0"
     gene            20703..>20978
                     /locus_tag="LQ159_RS25370"
                     /pseudo=""
     CDS             20703..>20978
                     /locus_tag="LQ159_RS25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /name="LysR family transcriptional regulator"
     gene            complement(21032..21736)
                     /locus_tag="LQ159_RS25375"
                     /old_locus_tag="AI2853V1_4899"
     CDS             complement(21032..21736)
                     /locus_tag="LQ159_RS25375"
                     /old_locus_tag="AI2853V1_4899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            21761..22321
                     /locus_tag="LQ159_RS25380"
                     /old_locus_tag="AI2853V1_4900"
     CDS             21761..22321
                     /locus_tag="LQ159_RS25380"
                     /old_locus_tag="AI2853V1_4900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749966.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004519 - endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="WP_230163698.1"
                     /translation="MISLSPPTICNSAQQSARRVLERIKTLPGFPQKINYLRKIDPFVF
                     EELLLEGFEAHGFRTIRNKRYTGDGGIDGQVIIGKYRYLIQAKRYRGHIALQHVQEFEK
                     LLKRHNCRGLFCHTGKTGAGSKSVSIASERMEIISGQRLIDLLTPGSSFTIATAPQTMM
                     KRTAATLETSTIVKDAGVMTPTY"
                     /name="restriction endonuclease"
     gene            complement(22315..23012)
                     /locus_tag="LQ159_RS25385"
                     /old_locus_tag="AI2853V1_REPA000000303"
     CDS             complement(join(22315..22763,22763..23012))
                     /locus_tag="LQ159_RS25385"
                     /old_locus_tag="AI2853V1_REPA000000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(23087..23368)
                     /locus_tag="LQ159_RS25390"
                     /old_locus_tag="AI2853V1_4903"
     CDS             complement(23087..23368)
                     /locus_tag="LQ159_RS25390"
                     /old_locus_tag="AI2853V1_4903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(23358..23609)
                     /locus_tag="LQ159_RS25395"
                     /old_locus_tag="AI2853V1_4904"
     CDS             complement(23358..23609)
                     /locus_tag="LQ159_RS25395"
                     /old_locus_tag="AI2853V1_4904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            24843..25679
                     /locus_tag="LQ159_RS25400"
                     /old_locus_tag="AI2853V1_4905"
     CDS             24843..25679
                     /locus_tag="LQ159_RS25400"
                     /old_locus_tag="AI2853V1_4905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001050931.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="RepB family plasmid replication initiator protein"
     gene            25719..26165
                     /locus_tag="LQ159_RS25405"
                     /pseudo=""
     CDS             25719..26165
                     /locus_tag="LQ159_RS25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            26302..26658
                     /gene="ddp1"
                     /locus_tag="LQ159_RS25410"
                     /old_locus_tag="AI2853V1_4908"
     CDS             26302..26658
                     /gene="ddp1"
                     /locus_tag="LQ159_RS25410"
                     /old_locus_tag="AI2853V1_4908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000456533.1"
                     /translation="MEYQVREFINEKYTKAVNILKDNLKENYHVFYGVRLIEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKILDASLLEGTNIVVLECITLA
                     DLLTNDNIDFLYKS"
                     /name="ddp1"
     ARG             7657..7985
                     /name="qacL"
                     /gene="qacL"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6696..7483
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11721..11974)
                     /name="mef(B)"
                     /gene="mef(B)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="20.54"
     ARG             6582..7486
                     /name="aadA3"
                     /gene="aadA3"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.48"
                     /coverage="114.45"
     Typing          25720..26165
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.21"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttttaaac gttgctgtgg tcagtttatg gtgatctgtc aacctgtaga ggtcacagat
       61 caattcggtt aaatatctca tagattttca tccatgcttc tatggttaga attttgcaga
      121 ctcaagcaac cagtgattct ttatgttttc atcctggctg ttgtacatcg ccagcgccgc
      181 tttgagaacg gttgttttgc tctgccctgt tctgatagct tcagcttcca gtatgtcctc
      241 aaaatgggct gaaagtctga agttcatcaa ctttggttta cccactacag gcgctctggt
      301 agcgccggag ataaaacgcg cggtttctgc gtttgttgct ggttgctgag attcatcgat
      361 gttcggacgg tttagtttta aagccatgac aatttcccct tgtacggtat gcattaatag
      421 taaatatcat actatcttta tattatcttt acaatgtaaa gatttataga tggtattgat
      481 attactataa aattccaatt tcctgcgcca ctagttcaat ctgtgctttc gctgttgaaa
      541 ggcttgatgc ccggctaacg tcatgtaccc ctgccccttc attacaggcg ctttcataca
      601 catccagctc ggatatccgg gttttaagag gttgaatcca gatgttgtcc gaacgcagta
      661 atttatccag atcaatggcc gcttttctgt ggcggggttt cgctgagttg attctggtga
      721 acagcaccca cggttccagg gctgcgttag ctttctgagc ggtgcggatt ttctctgtaa
      781 cactggtcag agtttcgctt tcgaaatcag aggacggctt aaccagtgtt accacgatat
      841 ctgatactgt cagggcgcta cggaactcct ggctgtcgtg gccggggcaa tcgacaatga
      901 ggacttcaca cagcttacta agtcgcttaa tttcattgct gacgtttccg taagctgcgt
      961 gaaccgggat agtgagtaag ccgttatctg tccgcttttc gttccagctc aacagatcgt
     1021 ggtttttatc tgtcttgagg ataattaccg acttaccttt attgaccagc attgagcctg
     1081 tgttagccac gtaggtgctt ttgcctacac cacccttatc tgatacgaca agcaagattt
     1141 tacccatttc agaattcctc tctttatgac gtaaagatta tgtaaagact gtcttatctt
     1201 tacattgtgt gttgttcgta aagattattt tgtctttaca gtgtctttat attacacatg
     1261 atttaccatg taaagcacaa ataaagataa gcttgtattt atgttatctt tattgtgtaa
     1321 agatttgtgt gtatttatag tggctttacg tggtaaatct gagaccgtct ttacatcatc
     1381 tttatgttgt aaatttctgt gtgacattgt cactttatgg tcggaggtct gttttctggt
     1441 tctttttgag tgtgcctttc ctgtcgatct actttctggt gcattttggg tatagtggtg
     1501 taaccttatg tgaaccggtt ctgtaatatg acttcaaatg taccaacaag ctttgtccgc
     1561 gcttatctga gagcttcaac agcagaacag gatgcttcgc gtgccctgga aactatcgag
     1621 gcttttgcca gggaaagagg cctgatcatt tgcagctact atattgagaa tgaatccggc
     1681 acccgtcttg agcgcccgga actttttcgt cttctgagag attgccaaca aaatgacatt
     1741 ttgcttatcg aggatgtgga cagactttcc cgcctgtcag gggaagactg gaatacgttg
     1801 aagaaaatga tccggcagaa agacatccgg gttatggcgg tgaatgtgcc gacgacatgg
     1861 attaattctg gtatgagtga gtttgacagc cggttgtttg cagccattaa cgatatgctg
     1921 ctggatatgc tggccgcagt tgccagaagg gactatgaac aacgtcggga acgccagaaa
     1981 caagggatag agaaagcaaa gaaagacggc aaatataaag ggcgtaaacc taatcaagcc
     2041 agacatgatg caattatcag gttgatagaa agcggtagct cctggacaca ggtacagaaa
     2101 gtgctagggt gcagccgtgg cacaatcagt aatgcgctaa aacgtaaaac cgggcagaaa
     2161 tccagtaagt agaggagtag ggggctaata tacctgcaag gcaggatatt aactcaggag
     2221 attgggtgtg tggtgtttag tcaatgcatt tgaatattgt aactaaaata tgtttttatt
     2281 aaaggccgga ggtggttagc ccccggcctt agttacgttt ccgccatcag cccatcaact
     2341 ggagaaggta gcgaatcaat tcaagaagtg agattagtgc ccccagaacg atgattgctt
     2401 tatcaatcat ccgtttactc cattatatgg agcagactcc cgatacttac tcttacactg
     2461 cctgtcatat cggttgttct tcggtgttgg ttgaagacag ttccactcca gccagagcca
     2521 ccaactcagg actggaaaac acgaaaaagc ccgattacca gtcgggtttt tttgtgcctg
     2581 tcgttcgact gttcttccgt tttttccctt catttggggg atttttcttt cattttttgt
     2641 tgcgaccaat actgctgttc gcgttggcca actgtgtaat ggcgttactg acatttaagt
     2701 ccattgtcag aaataacacc gttcagaaca tctatataag ggcatgtaca gaagtaagct
     2761 taacgattaa cgaacattta gatcaaaatc tcttagctga aattattggt aaaataagga
     2821 agtaaaggca ggtgttctct aatgctttag atatttacgt ggtgtacact catagtattg
     2881 tctgaaaagg catacaaagt acgatgtact actaaaccca cacatttcag acacatattt
     2941 tatcggatga ttttgcttta acaatttaat ggcaagcgac atcctggtat caagtaatat
     3001 tttactgaaa gaagtgtttt ctaataatag ctttttcttt attaagctct cgctcatgta
     3061 aagataatca gctaaatctc tcaatttcca gtgtttagaa acatctgaaa gaagaatgct
     3121 tcgaactttt cctgaaaacg taggcatgcc tgatgtgagg aatgctctta agtttgcggt
     3181 aatgactcca acttattgat agtgttttat gttcagataa tgcccgatga ctttgtcatg
     3241 cagctccacc gattttgaga acgacagcga cttccgtccc agccgtgcca ggtgctgcct
     3301 cagattcagg ttatgccgct caattcgctg cgtatatcgc ttgctgatta cgtgcagctt
     3361 tcccttcagg cgggattcat acagcggcca gccatccgtc atccatatca ccacgtcaaa
     3421 gggtgacagc aggctcataa gacgccccag cgtcgccata gtgcgttcac cgaatacgtg
     3481 cgcaacaacc gtcttccgga gcctgtcata cgcgtaaaac agccagcgct ggcgcgattt
     3541 agccccgaca tagccccact gttcgtccat ttccgcgcag acgatgacgt cactgcccgg
     3601 ctgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa aatcgtgttg
     3661 aggccaacgc ccataatgcg ggcggttgcc cggcatccaa cgccattcat ggccatatca
     3721 atgattttct ggtgcgtacc gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt
     3781 ttacggcagt gagagcagag atagcgctga tgtccggcgg tgcttttgcc gttacgcacc
     3841 accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct
     3901 caaaaacacc atcatacact aaatcagtaa gttggcagca tcaccgtgca tcttgtcgta
     3961 atagtgccgc caaaaatctc aatatctact ctgatggggc acctgaaagg tcgcagtgca
     4021 attaggctct acaatcgatt cccgcatatc aggaagaagt tatggggaaa ccatttttgg
     4081 tcgcggggtt attttgtcga tacggtaggc gtgaacgaag aaattatcag acgatatgtg
     4141 aggcatcagg aaaagatgga acaaacacat gaacagcaga tggagttgct agagtagaaa
     4201 acggaatatg acatttgccc cccttatagg gggcattctg aaaagccacc tcctcaggag
     4261 gtggtctttt actctatcaa caggctgtgt gatcaacaat ggtcctctct ctgacagttc
     4321 tgatttcaaa taatcagctt aaaaatacct ttcaaaagta aacaatttcc tctgagggca
     4381 cattttatct actttggcct gtaaagctgg cgataatatt ccagacgttg tagatataac
     4441 ggcacgtttt cctcaggaat atcggacgga attgtgttac ggggggggag ttttttcaaa
     4501 tactctcgga acgcctgggt tgatgccata aaatctatag cttgatctat aagatgcgga
     4561 atgtcattac ctattttttc catgataata tctccgtatt acttcgcacc agcggttaat
     4621 tttagataag atgctaaaag tagcattaag gaaaacgtgc ttaatcctca atgttgactc
     4681 ccggaaatct tttaactttt taagtttctt aatgaatcaa taaattacat agttcgtaag
     4741 caagtatatt attgcctgat taaactattt tgatgctatc aaccatggac aaagcacctc
     4801 gttgcacata acagagacta tgacgttatt tctgtcagag gccatcacat gaaagaagtc
     4861 aaaaaaaggg gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat
     4921 gactttgtca tgcagttcca ccgattttga gaacgacagc gacttccgtc ccagccgtgc
     4981 caggttctgc ctcagattca ggttatgccg ctcaattcgc tgcgtatatc gcttgctgat
     5041 tacgtgcagc tttcccttca ggcgggattc atacagcggc cagccatccg tcatccatat
     5101 caccacgtca aagggtgaca gcaggcggca ctgttgcaaa tagtcggtgg tgataaactt
     5161 atcatcccct tttgctgatg gagctgcaca tgaacccatt caaaggccgg cattttcagc
     5221 gtgacatcat tctgtgggcc gtacgctggt actgcaaata cggcatcagt taccgtgagc
     5281 tgcaggagat gctggctgaa cgcggagtga atgtcgatca ctccacgatt taccgctggg
     5341 ttcagcgtta tgcgcctgaa atggaaaaac ggctgcgctg gtactggcgt aacccttccg
     5401 atctttgccc gtggcacatg gatgaaacct acgtgaaggt caatggccgc tgggcgtatc
     5461 tgtaccgggc cgtcgacagc cggggccgca ctgtcgattt ttatctctcc tcccgtcgta
     5521 acagcaaagc tgcataccgg tttctgggta aaatcctcaa caacgtgaag aagtggcaga
     5581 tcccgcgatt catcaacacg gataaagcgc ccgcctatgg tcgcgcgctt gctctgctca
     5641 aacgcgaagg ccggtgcccg tctgacgttg aacaccgaca gattaagtac cggaacaacg
     5701 tgattgaatg cgatcatggc aaactgaaac ggataatcgg cgccacgctg ggatttaaat
     5761 ccatgaagac ggcttacgcc accatcaaag gtattgaggt gatgcgtgca ctacgcaaag
     5821 gccaggcctc agcattttat tatggtgatc ccctgggcga aatgcgcctg gtaagcagag
     5881 tttttgaaat gtaaggcctt tgaataagac aaaaggctgc ctcatcgcta actttgcaac
     5941 agtgcccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6001 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6061 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6121 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6181 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6241 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6301 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6361 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6421 ggcacgaacc cagtggacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat
     6481 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6541 ggttttcatg gcttgttatg actgtttttt tgtacagtct atgcctcggg catccaagca
     6601 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     6661 gcagggcagt cgccctaaaa caaagttaga catcatgagg gtagcggtga ccatcgaaat
     6721 ttcgaaccaa ctatcagagg tgctaagcgt cattgagcgc catctggaat caacgttgct
     6781 ggccgtgcat ttgtacggct ccgcagtgga tggcggcctg aagccataca gcgatattga
     6841 tttgttggtt actgtggccg taaagcttga tgaaacgacg cggcgagcat tgctcaatga
     6901 ccttatggag gcttcggctt tccctggcga gagcgagacg ctccgcgcta tagaagtcac
     6961 ccttgtcgtg catgacgaca tcatcccgtg gcgttatccg gctaagcgcg agctgcaatt
     7021 tggagaatgg cagcgcaatg acattcttgc gggtatcttc gagccagcca tgatcgacat
     7081 tgatctagct atcctgctta caaaagcaag agaacatagc gttgccttgg taggtccggc
     7141 agcggaggaa ttctttgacc cggttcctga acaggatcta ttcgaggcgc tgagggaaac
     7201 cttgaagcta tggaactcgc agcccgactg ggccggcgat gagcgaaatg tagtgcttac
     7261 gttgtcccgc atttggtaca gcgcaataac cggcaaaatc gcgccgaagg atgtcgctgc
     7321 cgactgggca ataaaacgcc tacctgccca gtatcagccc gtcttacttg aagctaagca
     7381 agcttatctg ggacaaaaag aagatcactt ggcctcacgc gcagatcact tggaagaatt
     7441 tattcgcttt gtgaaaggcg agatcatcaa gtcagttggt aaatgatgtc taacaattcg
     7501 ttcaagccga ccgcgctacg cgcggcggct taactccggc gttagatgcc agatttggcg
     7561 ttgcgtatgc tcacagaaaa tcgacagccg caagactgtt tttggcaact catagccacc
     7621 actatttatc cttcataccg tagaggagat tgcacgtgaa gaactggctc tttctggcta
     7681 ttgcaatatt tggtgaggtc gtcgcaactt ccgcactgaa gtccagccat ggattcacca
     7741 agttagttcc ttctgttgta gttgtggctg gctacgggct tgcgttctat ttcctctctc
     7801 tcgcactcaa gtccatcccg gtcggcattg cttatgctgt ttgggctggc ctcggcatcg
     7861 tacttgtggc agctatcgct tggatcttcc atggccagaa actagacttg tgggcgttcg
     7921 ttggcatggg acttatcgtt agtggcgtcg ccgttctaaa tctgctatcc aaggtcagcg
     7981 cacattgatc gggctggcat ctaacagttc attcaagccg acgccgcttc gcggcgcggc
     8041 ttaattcagg tgttagtcgg acgcctacgg cgccgaggaa aaaagaaacc aaaccattct
     8101 tgctgccaaa gagaaaaacc ccttacggtg gcgtactggg aaagtgcgta tctacgacgc
     8161 cttgccggtc tcaatgtcat gccgtcctct ttcgaaggct gcttgatgca aacctctcaa
     8221 aaatctggca taaactatct tgacatattc cgattgaaca ccttccgaat atcagcggct
     8281 atcagcggga tctcatcttt tttcgtgacg taggagtggg cattctgctg cagatgaaac
     8341 tggcagcgtt gccacagaat tccagggaag acagcatcga ttgccgcgcg cagtcctgag
     8401 tggtcatcac tggtgatcat tcgcaggccg tgcatccctc gtcgtacgag accctcgaga
     8461 aaggaacgcc agtttacctc tgcctcactg ttcgcgactt cggcgtcaag aatatgccga
     8521 ttcccgctgt aatcaacgcc aatagccact agaagcgcct gcttgaccac atgggaaccg
     8581 acgcgaaccg attcataggt cgcatcaagt accaagtatt gaatctgtcc gacaggctgc
     8641 gccttccaag acgtaatctc ttcatcgagc tcctttgcca gcctgctgac ctgggacgag
     8701 ctgacttcgg tgccacaaag aatttcgacg atatccgaga ccctgcgggt acttactcct
     8761 ttgacataca tttcggcgat ggcgagcttg agagcacgct cactgcgcat gcctttctcg
     8821 atgcagctgg gataaaactc catcccgcga acttgtggta ctttcaagag gactttcccg
     8881 gtcgcaagat tgaatattgg attatttgga tttaccaaca aacactacta tttagctacg
     8941 aaaactcaag agaagtacac aaaacctttt ggaagtgatg gaagtttaag tcctcttgat
     9001 tatagtgtag gtattggaac aggtttagaa ttaggacatt tccagtttat gtttggctat
     9061 gattaccctc ttagtaacag ttcgaaagat ccagacctaa gtcaagtacg ccaacacaac
     9121 ttcagagcaa gtatagcata ttcatttaga aagctaaaaa aataaaacta acctagggct
     9181 ttggatattt tcaaggcatc tgataaagac ttaacatcat gggtgcggag ataatctgca
     9241 ccttttttgt atgcatacat ttctgctgca agagttggtg ctaaacgaga tttcacatcg
     9301 gttccagtta ttttacctaa gaatgatttc cgtgacactg caatcattac ttgcaaatta
     9361 aaagcttctt gaatttcagg gaaacgcttc aaaacaagaa tagatgtttc tggattagag
     9421 cctaaaaaga agcccatacc cggatcaaga ataattcgtt cacgctttac accagcctca
     9481 actaaagcag caattctttc tttaaaaaat tccatcatgg aagtaaaaac ctcttccgga
     9541 ttcgtttcaa ctttagtagc tgcaccaatt cgctgaacgg agtgcatcaa cacaagtttg
     9601 caatctgact ttgccaagcc tgaataaatc tcaggataag gaaaaccttg aatatcatta
     9661 ataaaatcaa ccttttgttc tatgcaaaaa ctctgaacct caggtttaaa tgtatcaaca
     9721 gaaatagaaa tgcctttttc ttttaaagcc ttaatgacag gtttgagtct tttgatttct
     9781 tccacaacgc ccacttcagt tgtatcagga ttactggaag cggctcccaa atcaatcaca
     9841 tctgctccat cttcaaccaa atgcagagca tgctcaattg ccttatctgt atctaaataa
     9901 agtcctccat cggaaaaact atcggtggtt atatttacga ttccaaaaat cttgctcata
     9961 tcattttgct ttgattacaa aagtagcatt tatcttaatt gactaattat cacttacgca
    10021 aattgacaca ttttccctat ctttgcagcc taattttttt gttttaattc attatgaaaa
    10081 ctgataggaa ctgtcatttc gagtttttcg cggttttggg tcaaaattcg gttttttccc
    10141 cgaactacga ctgttttcgc accccgaaca actaatccaa tcagaatttt ccggcaacgc
    10201 cgactccaag aagtgtaatt ccagtctatt tctgccgtca acccctgatt cgcgttaatg
    10261 cgacaaagct caaacacata actcccccac gaagccatat aaactccgat ttcgaggtgt
    10321 tatcctgttc cgcataaact tcgtttttgg gtattatacc tacaccaaag caagaagatt
    10381 atgggtccaa acttcatttg tatattttgc aaacaaatat ctgcctgtca tatctgtcgg
    10441 agtgtggtga tactcctttg cattaaaaat gcgctgataa aaggtagttc agcaaaaggc
    10501 gaaacgccaa taaatatatc tataaccggt ttcgcccgtt gattgggtat cgccgtgctt
    10561 ccgatatgtt caatggctac ggcatgagaa ccgaaaaaat ggagcaattt tattttctct
    10621 tcttcaaaat tctttgtcca atccgggtta tattccataa tttcaatttt catgatagtt
    10681 tcgtccttgc ctcaaaaacc atgtttggta ttatactcca tatctctccg gtgcaggaat
    10741 agccttttct gatgcacggc taacagcttc ccattcttcc caggtagcca tccgtgcttt
    10801 taaggtatcc atcgccaaat cgtataccat gctgccaagg ataagcctca aaggcggatt
    10861 attgctggct actaatttca taagggcttc cgctgccaag gaaggatcac tatcgaccga
    10921 atcttcggaa tactgtttcg ccagctcatc gcgcagtgta ccgtatgaat ctaatggatt
    10981 gctgtaactc atcgaagtgt ataaatctgt ccaatatcct cctggttcca ccatagtgag
    11041 tttcactccg aaatgttccg cttcttttgc taaggcttca ctcattcctt ccagcgcaaa
    11101 tttacttgca ctgtaaatac cagacatcgg acccgagata atagcgccaa tgcttgtaat
    11161 ctgtatgata tgtccagaac gctgcgacct caaatagggc atcactgcct gacaaaccca
    11221 aagagctcca aaaaagtttg tgtccattag tttccgagca tcggattcgt ttagttcttc
    11281 aatcataccc ataatcatga tacccgcatt attaacaaca atatcaagcc taccgaaatg
    11341 tttaactgct gtttcaaccg tagaaaaaac agcctcccta tctgtaacat cgaggtttaa
    11401 tggcagtaag ctctcttgat atgtttcttc tagcttcgcc aaattatcga tagtccttgc
    11461 aactgcaaca actttatccc cggctttcaa ggcggcgcat gtaaaagcat agccaagccc
    11521 cttgcttgct ccggtaataa accatactct tttattttcg ctattaggta tcatagatat
    11581 tctcctttag gctattatag aaagattatt ataagcgaaa ttaataccgt taaacgccat
    11641 gagcccgaca attttgagca ctttgttttg tactcccaga agctcaccac tacaaattat
    11701 catcaacccg gtcctttttt cagtccactt gcggtttcat tgttactttg tcatagcgtc
    11761 gtgtcagaat gcggcagaga acagcgttta ctatcaacgc aacaccagac cagaaaaacc
    11821 atgtattaac acctataacc tcaacaaccg gacctgcaac aagtaagcct atcggcatag
    11881 aaagagtcat ggcggtcatc aaaagggaaa acaccttgcc catcatttca ggggcaatgc
    11941 tttcttgaac ataagccata acaggaacat tcataaatgg gcactgttgc aaagttagcg
    12001 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
    12061 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
    12121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
    12181 tggcgccgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
    12241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
    12301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
    12361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
    12421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
    12481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
    12541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
    12601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
    12661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
    12721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc
    12781 gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag
    12841 tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt
    12901 ggggttaccc ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc
    12961 gaagatgggc cacttggact gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg
    13021 ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc
    13081 gttacaccgg accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc
    13141 agcgcccatc catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat
    13201 ccattgcccc tgccacctca ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat
    13261 gggcaggtac ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag
    13321 ttgatggcaa aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag
    13381 ttggtacgcg tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac
    13441 aggtggctca aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg
    13501 ttgatccgct cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg
    13561 atcttcctgc atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt
    13621 ggctgagctc atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct
    13681 ggggcaacac gtggagcgga tcggggattg tctttcttca gctcgctgat gatatgctga
    13741 cgctcaatgc cgtttggcct ccgactaacg aaaatcccgc atttggacgg ctgatccgat
    13801 tggcacggcg gacggcgaat ggcggagcag acgctcgtcc gggggcaatg agatatgaaa
    13861 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
    13921 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
    13981 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
    14041 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
    14101 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
    14161 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
    14221 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
    14281 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
    14341 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
    14401 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
    14461 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
    14521 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
    14581 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
    14641 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
    14701 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
    14761 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
    14821 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
    14881 agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca actcgcgcga
    14941 gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca
    15001 gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt caggcccttc
    15061 tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt gatctgactg
    15121 atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc ttgcgcaacg
    15181 agatgccggc atacgatccg caaaccacac caactcggaa catgggtgca gtggctgaat
    15241 tgttccgggc gtgtcgcaaa cacttttgga ggcagctatc acccagccaa ggccggtatt
    15301 ttcatgcttt gttaaccttt tgcagtccga agaggcgcgc aagcaaatca ttctggtcat
    15361 tgacggtcca gccgcctgaa aagccaaagc ccttccgcag ccacagcatc gcctcgaaac
    15421 ccgcgatggt tcgccgcgcc gtgttaaagg attggaaacc gccgatcttc ggcatgttct
    15481 tcttcacccg gaaatggtcg ctctcaatcc cctgctggag atgtttggtc acataatgca
    15541 cgggatcggg atggagaagc ccatcatcaa ccgacgtttt gatcgttgac gggaaggtgt
    15601 tggccccgtc cgtcccaatc ctgttcggcg acaacaaggg ttcatcttta agcatctttc
    15661 ggaagaaccg cttggcagcg tcgagatcgc gcttagcggt cagcaggaaa tccaccggat
    15721 tgccatgctt atcgatggct cggtacagat agcgccattt gccgcggatc ttgacatagg
    15781 tctcatcaat ccggaccgag ccgcaatggg gtcgacgaaa ctgccgcagc cgtttctcga
    15841 tgacaggtgc ataagccaat acccagcggt tgatcgtgct atgatcgacc tcgaagcccc
    15901 gctcgcgaaa catctcttcc aagtcacgat agctgagcgg gtagcgcaag taccaggcaa
    15961 ccgcctgtac aatcagccag gcctcgaaat gcctgccctt gaaatcatcc ttcgactggc
    16021 gcttcagctt ttcggcaatg gtattcaaaa tcatcggctc gctccgcaat atcaggagcg
    16081 gcaacttctc tccccatggt caacatcgag ttaacctgaa gaatttgcga caaggccatg
    16141 cgttccgctc cggagatgtc tggcttacta ggtcccggcg ctatggcgat ctgaaacacg
    16201 cactcgttcc ggcacaatcc atcgcggaag gcggtcgtct cgctgtgcca ttgcggccgg
    16261 aggaatggct ggcagaccgg caagctcgcc tcgacatgcg gttgcgcgag cttggccgtg
    16321 ccgctcgcgc aggcacgatc ccgggcgggt cgattgaaaa cggcgttctg catatcgaga
    16381 aactcgaagc cgccgcgccg acaggcgccg aagatctggt gctcgatctc tacaagcaga
    16441 tcccgcccac gcgcatcacc gatctcctgc tggaggtgga tgcggcgacc ggcttcaccg
    16501 aagcgttcac ccatctgcgc acaggagcac cctgcgctga ccggatcggg ctaatgaacg
    16561 ttatcttggc ggaagggatc aacctcggct tgcgcaaaat ggcggatgcg acaaacaccc
    16621 acaccttctg ggaattgatc cgcattggac ggtggcatgt cgagggcgaa gcctatgacc
    16681 gggcgctggc catggtggtc gaggcacagg cagcgttacc catggcccgg ttctggggca
    16741 tgggcacgtc ggcttcgagc gacggacagt tcttcgtcgc tacagagcaa ggtgaggcca
    16801 tgaacctggt caacgcgaaa tatggcaata ccccgggcct gaaagcctat agccacgtct
    16861 ccgaccaata tgcgccgttc gcaacccagg tgattcctgc aacggcaagc gaagcgcctt
    16921 acatcctcga tggcctgctg atgaacgatg ctggacgcca tatccgcgag cagttcaccg
    16981 acacgggcgg cttcaccgat cacgtctttg ccgcatgtgc cattctcggc taccggttcg
    17041 ctccgcgcat ccgcgacctg ccatccaaac ggctctacgc gttcaatccg tcggccgccc
    17101 cggcgcacct gcgagcgttg atcggcggaa aggtcaacca agccatgatc gagcgcaatt
    17161 ggcccgacat cctgcgcatc gccgccacca ttgctgccgg gaccgtcgcg ccaagccaga
    17221 ttctgcggaa actcgcctcc tatccgcggc agaacgagct cgcgacagcc ctgcgggaag
    17281 tcggtcgcgt cgagcgcacc ctgttcatga tcgactggat tctggatgcc gaactccaac
    17341 ggcgtgccca gatcgggctc aacaaaggcg aagctcatca tgcgctgaag cgggcaatca
    17401 gcttccaccg ccgcggtgaa atccgcgacc gttccgccga aggccagcat taccgcatcg
    17461 ccggcatgaa tctgctcgcc gccatcatca tcttctggaa caccatgaag ctcggcgagg
    17521 tcgttgcaaa ccagaaacgc gatggaaagc tgctatcgcc cgatctcttg gcccatgttt
    17581 cgccgctcgg atgggaacac atcaatctca ccggagaata tcgctggcca aagccttagc
    17641 gtaggattcc gccccctccc gcaaacgacc ccaggcgggg cgaaaggtgt tcactttgca
    17701 aactctgccg accagtggtg atccgttcgg tgacgggatt ggcaaggtag ccgggtccag
    17761 cctgcacgcc ggcgtggcgg ccagggccga tgaacgcaag aagctcgaac ggctgtgccg
    17821 gtacatcagc cgcccggcgg tatccgagaa gcggctgtcg ttaacacgag gcggcaacgt
    17881 gcgctaccag ctcaagacgc cgtaccggga cggcaccacg cacgtcattt tcgaaccatt
    17941 ggatttcatt gcaaggctgg ccgccctggt accgaagccc agagtcaacc taacccgctt
    18001 ccacggggtg ttcgcaccca acagtcggca ccgggcgttg gtcacgccgg caaaacgggg
    18061 caggggcaac aaggtcaggg tggctgatga accggcaaca ccagcacaac ggcgagcgtc
    18121 gatgacatgg gcgcaacggc tcaagcgtgt tttcaatatc gacatcgaga cctgcagcgg
    18181 ctgcggcggc gccatgaaag tcatcgcctg cattgaagac cctatagtga tcaagcagat
    18241 ccttgatcac ctgaagcaca aagccgaaac cagcgggacc agggcgttac ccgaaagccg
    18301 ggcgccaccg gctgagctgc tcctgggtct gtttgactga cgagcctgaa ggccaacgat
    18361 accaatcaaa atgctgcgtt cacagcgccg cggcagggat ccgccgtgct ggttgtcgga
    18421 aaaggagccg ctagtgggaa agaggagggt aaattttcag cgttgctggc tccccgtcag
    18481 ccggattggg ttgcatcgca ggggtgtcga aagagtcaac tgcggtccaa agctgttgga
    18541 cttgggtgaa aagggcgttt attcttccta tacgtgccgc gttccaggcg gcgactatga
    18601 gggctatgtc gatgcccatg tgcgcgctac gccgggccgg tatcgtcgag cggatcgacg
    18661 ccgaccaatg gcgcatcccc gatgatctgg tcagccgtgc cgccgcccat gacgccggcc
    18721 gagacagtca ggccagcgtt cgcgtccttt ccccggtcga tctgaacaaa cagatcggat
    18781 cggacggcgc gacctggctg gaccggcggc tgatccacgg cgagacggcc gaccttgcgc
    18841 caaccggctt cgggcaacaa gtccgcgaag ccatggacca gcgccgcgag caccatatcg
    18901 aacagggcga cgccacccgc agccgggaca gccgcgtctt ctaccggcgc aaccttctcg
    18961 ccatcctgcg ggagcgcgag gtagccggcg tcggatcgga tatggctttg agtaagggcc
    19021 tgccgttccg cgccgccacg gacggcgaga gcgtcagcgg caagtttacc ggaaccgtgc
    19081 atctatcgag cggcaagttc gccgtggtcg agaaatccca tgagttcacc cttgtcccgt
    19141 ggcggccgat catcgaccgc caactcggcc gcgaggttat gggcatcgtg cagggcgggt
    19201 cggtgtcgtg gcagttaggg cggcagaggg ggctggaacg ctgagtgcgc ccatgccgca
    19261 ttgcgaagca aaagataatc ggataaaatg tagcaattca tattcgtaag cgtggagtaa
    19321 tcagatggga aattccaagt cagcagacaa gtaagccgca acaaccagta ttgttgttgc
    19381 ggcgctctgt aaggctagtc tcatctgatt gctgacgagc agacgtcgcc cggtattcct
    19441 taatcgaggg ttgattcgtc atgaccacca cacgccccgc gtgggcctat acgctgccgg
    19501 cagcactgct gctgatggct cctttcgaca tcctcgcttc actggcgatg gatatttatc
    19561 tccctgtcgt tccagcgatg cccggcatcc tgaacacgac gcccgctatg atccaactca
    19621 cgttgagcct ctatatggtg atgctcggcg tgggccaggt gatttttggt ccgctctcag
    19681 acagaatcgg gcgacggcca attctacttg cgggcgcaac ggctttcgtc attgcgtctc
    19741 tgggagcagc ttggtcttca actgcaccgg cctttgtcgc tttccgtcta cttcaagcag
    19801 tgggcgcgtc ggccatgctg gtggcgacgt tcgcgacggt tcgcgacgtt tatgccaacc
    19861 gtcctgaggg tgtcgtcatc tacggccttt tcagttcgat gctggcgttc gtgcctgcgc
    19921 tcggccctat cgccggagca ttgatcggcg agttcttggg atggcaggcg atattcatta
    19981 ctttggctat actggcgatg ctcgcactcc taaatgcggg tttcaggtgg cacgaaaccc
    20041 gccctctgga tcaagtcaag acgcgccgat ctgtcttgcc gatcttcgcg agtccggctt
    20101 tttgggttta cactgtcggc tttagcgccg gtatgggcac ctacttcgtc ttcttctcga
    20161 cggctccccg tgtgctcata ggccaagcgg aatattccga tatcggattc agctttgcct
    20221 tcgccactgt cgcgcttgta atgatcgtga caacccgttt cgcgaagtcc tttgtcgcca
    20281 gatggggcat cgcaggatgc gtggcgcgtg ggatggcgtt gcttgtttgc ggagcggtcc
    20341 tgttggggat cggcgaactt tacggctcgc cgtcattcct caccttcatc ctaccgatgt
    20401 gggttgtcgc ggtcggtatt gtcttcacgg tgtccgttac cgcgaacggc gctttggcag
    20461 agttcgacga catcgcggga tcagcggtcg cgttctactt ctgcgttcaa agcctgatag
    20521 tcagcattgt cgggacattg gcggtggcac ttttaaacgg tgacacagcg tggcccgtga
    20581 tctgttacgc cacggcgatg gcggtactgg tttcgttggg gctggtgctc cttcggctcc
    20641 gtggggctgc caccgagaag tcgccagtcg tctaaccgac gactggtagc aggcccgctc
    20701 cgatgcggcg cactaaccat cgaaacctcg tgaatgtcgg tatcctgtct ggcaggatac
    20761 cgctcatttc ccttgttcag ttcatcgccg tcgccgagca tctgaatttt cggcatgcgg
    20821 ccaaggcact tggtatcagc cagtcgagcg tcagcgcgcg tgtgaaagcg ctggaggata
    20881 accttggtgt cctgctattt gagcgccatg cgcggggcgt tcggctaaca gacgcaggca
    20941 ggcacttcat ggagcgtgtc acggcgggtg tcgatcaacg gcactgttgc aaagttagcg
    21001 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
    21061 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
    21121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
    21181 tggcgttgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
    21241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
    21301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg cgggatctgc cacttcttca
    21361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
    21421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
    21481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
    21541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
    21601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
    21661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
    21721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc
    21781 gactatttgc aacagtgccc agcaaagtgc gcggcgggtg ctggagcgca taaagacgtt
    21841 gccgggcttc ccacaaaaaa ttaactacct gagaaaaatt gatccttttg tgtttgaaga
    21901 actgttgctg gaaggatttg aagcgcatgg cttcagaacc atcagaaaca aacgctatac
    21961 cggcgatgga ggcattgacg gccaggtaat aataggaaaa tatcgctatc ttattcaggc
    22021 taaacgctat cgcggccata ttgctttaca gcacgtacag gagttcgaga agttgcttaa
    22081 acgtcataac tgtcgcggtc tgttttgcca taccgggaaa accggcgcag gttcaaaatc
    22141 tgtcagtatt gccagtgaac ggatggagat tatcagcggc cagcgcctga tagatttgct
    22201 cacgcccggt agctccttca ctatcgcaac cgccccgcag acgatgatga agcgtaccgc
    22261 agcaacgcta gaaacgagca ccattgttaa agatgccggg gtaatgactc caacttattg
    22321 atagtgtttt atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga
    22381 gaacgacagc gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg
    22441 ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgggattc
    22501 atacagcggc cagccatccg tcatccatat caccacgtca aagggtgaca gcaggctcat
    22561 aagacgcccc agcgtcgcca tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg
    22621 gagcctgtca tacgcgtaaa acagccagcg ctggcgcgat ttagccccga catagcccca
    22681 ctgttcgtcc atttccgcgc agacgatgac gtcactgccc ggctgtatgc gcgaggttac
    22741 cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt tgaggccaac gcccataatg
    22801 cgggctgttg cccggcatcc aacgccattc atggccatat caatgatttt ctggtgcgta
    22861 ccgggttgag aagcggtgta agtgaactgc agttgccatg ttttacggca gtgagagcag
    22921 agatagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa
    22981 caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca
    23041 ctaaatcagt aagttggcag catcaccaat catttgatga ttaatcttag cgcaaatctg
    23101 cgatctgata gacttcgctg ctttcccttc ttccaacggc aataacgagt aaaacgactt
    23161 tctcatcaat gacttggtat acaagtctgt agccagatgc acgaagtttg attttatagc
    23221 gatcagcatg accatgtaac cgggctgcgg gtacgcgtgg attttccagc cgttctgcca
    23281 gtttcttttt gaattgttca cggatggtgt ggccgagttt ctgccattcc ttcagtgctc
    23341 tacggtcaaa agccagttca taggtcatcc aggttaacct tcacaatttc cgggtttttc
    23401 atgcgctcat cagcaacagc gtttaactca gcatcttcag cgagttcccg atagtaggcg
    23461 taaagttttg gtggaacgca atagaacgcc ggttcgtttc ggttcaggat cgcaacagcg
    23521 tccccttcac cttcagctac ggttcccata ggattttttt tcaggtcagt aatactggcc
    23581 gctgtagttg tcagaatttg atagctcata agccctccgc ttgtctcatt ggcataatag
    23641 catctttaaa agcactctta aaagtgctta tgttgagaaa aacatcagaa caggtgagat
    23701 atggctcttt ccgacatcgc cttaaaacgc tctgtaacgc gttctgagcg ttttttcccg
    23761 tttcgttgta gtcagctacg gtttaacggt ttttaacgcc gctcaacgct ttttagcggc
    23821 cttgttgctg tgatctgttg ccatgatccg gataggagtt caatcttgat caaaatttcc
    23881 tcaaggcaac gactgttttg taaataccct ctaaaacgcg ctgtatcgca ttctgagcga
    23941 ttttttcgat ttcgcatggt ttcaccatgg ttgtatcgaa cgaagcggca gaaagcgatt
    24001 cagggcttct cagtgaaaga tccaaaaagg atcaatcaac ggatcgtatg agctgaactc
    24061 ttctggtttt gagaaaggag acaccgctcg ccgcagccga acgaccgagt gtagcgagtt
    24121 agtgagcgag gaagcggaat agcacaggga ttacattagg cacttacgca cagtggttaa
    24181 aaaatgagca tattgttttt atgtgtggtt gtcttggttc agagtgatct gtcctccggg
    24241 tttacgcaaa gttatccaca ggcgctgtga attttttgtt ttgagcagag ttatccacag
    24301 attttttgtg tttttttgct tattaaactt cttgttttta agatcttaaa tgtagatcgg
    24361 tagatctact ttaagatctt taacttactt taacttattc tgcaaacatt gtgccagctt
    24421 ttttatcaat aatttcatta agataggtgt tgttttttgt cgcctcaaaa tgaaaggtgg
    24481 atgggtacgc actgaaaggt ggatgggtac gcactgtaat tttcatgaaa ggtggatggc
    24541 tgcgcactga aaggtggatg ggtacgcact gaaaggtgga tgggtacgca tttttctgtt
    24601 tttattcaga tattcgtatc tttgtatctt catttcttta gttctttata gtgctctaat
    24661 ttaacgtatg acgttaaaag gtggatacct gcgcatttct atcatattga atataaatga
    24721 taaaaattct ttcatgtgat ccgtgttttg gtggaaatac ataaggtgga taaaccactt
    24781 tacatatttc cgcctttatg gttatgattt caccatcaga acttaaaaac cacaggtaat
    24841 gtatgaactt actgagcgtt acaaagaaaa caaaaattcg ccacagaaat gaaattaaca
    24901 gcacattctc atcgttgcct ttgtctgcca gaagaatttt gtttatggct atggcgcaga
    24961 ttgattcacg tgaagagtta tctgaggggg caacctttcg tattacggct cgggaatatg
    25021 catttatcgc tgatattgat gtaacaggtg cttaccggca gttgaaagag ggggcagaag
    25081 aacttcaggc cagtgttatt cgcattcctc gaaatcaact tctcacacct cagggcaatc
    25141 aaagctctgg acatacgaag aagagaggta aattaccttc tgatgcagtc aggcttatga
    25201 acctgactgc gttttgtgat tatgttgaaa gtgaaggata tattgatatt gctttttcac
    25261 atgtcattga gccatatatc acgatgttaa aggattctta cactactcag gttttgatat
    25321 cgtctgtaag actcgctgat caaaattcaa atatgcttta tcagtttatc aggaaacaat
    25381 acagcagtgg taaaaagaca tttttcgaca ttgaagtaga tgtattaaag gatgaacttg
    25441 aattattcag aatagataat ggcgaaaaag tttaccttta tcagaatttt aaggatttta
    25501 ataaggtttt tctgcaaaaa aatattgaaa aaataaatca atatacagag attaatcatc
    25561 tcgaagttaa aatcgttgag agagtcgcaa gaagagcatc aaagctaagg ttttcttata
    25621 aaatagataa agaaagtgag ggcttagata tcagaatacc atacggtttc agaggataat
    25681 tgcataaact tcgttttgag atcgaaatga ggtagctcat ggctaaaatc tatcaattcc
    25741 ctcaggggga agaacgtgct aaattcagga aagaaattgc cagggagcgt aagaaacgtt
    25801 ttgcagtgaa aacagggagc acttttgtta agtggcttgg ctgaggatgg ttttatcttc
    25861 gtcttcttgt cgcgagtgtt ttacatttta tctttgtttt tgtgttagcc attcttggtg
    25921 cgtttaaatg ggctgtattc tggctgggtg gtattttgtg tgtcatcact tggtatcatc
    25981 tcgatcacca gttatggacg ccacaaaatc tgactatacc ggtgattacg gcaacctgga
    26041 ttttgggact gctggctgag cctctgatgg agctgctcaa caaaactatg ccttggcaca
    26101 agttacttgt tcccgaacct gcggacgaaa gcaccgagaa cagtagcgag ataagccagc
    26161 aatgatgcta ctcagcagtt atgccagtcc ctgccttttc actgcgatgc aggatagggc
    26221 aaacgccgca aaatgacgtc tctgacgcca ttctgcaacg tttgcccggt gacctacagt
    26281 cggttcttgt cggagaattt tatggaatat caggtgagag agtttattaa cgaaaaatat
    26341 actaaggctg ttaatatttt aaaggataac cttaaagaaa actaccatgt tttttatggt
    26401 gtgagattaa ttgagattct ttttccagcc agtgagtatg gcactgatgc tttctttaaa
    26461 gagtttgaat taattaacag tgtaatactt ccattggtaa tctttgattt aacacaacgc
    26521 aagccaatga tgattatcag cttcgataag atacttgatg ccagtttgtt ggaaggaaca
    26581 aatattgtgg ttcttgaatg tattaccctc gctgatcttc tgacaaatga taacattgat
    26641 tttttatata aaagctaaga atctcctttc agtttaaatt ttctgcttgg gtcgccaagt
    26701 gttattgagt ctacttcatg tgagtaatga agtgtatgat aatgtgctgg atttttcaga
    26761 taatcaggga gtgatattat ttttccgcag ctaattgcag aatgaaacca gttgcggaat
    26821 gtctcgctat ttgtttttaa tgcatctttt ggaataaata ctaattttat gttggaaacg
    26881 taattaacag gtttaagtat ggtatgtctg ttttgtccaa agtcttttaa agcatctgta
    26941 aactcgtatt cagatgtata aattctagtt tctttattaa gggttttgat ctggaatatg
    27001 aatgaatagt aattcttatc aagtatttca acagtgcctg ggtctatact cagttgttta
    27061 ataacagctt ctcttttata ctctaacccg tctctgactg attgatatac aaccattgct
    27121 gcatgatgtg agccaccact gtttgaagct acatatcggt caaaccaaga ataatattta
    27181 acattaatag gggggcgtgg ggggaaacct tcatgctcaa tgtgccatat attatctttc
    27241 cagtctaaaa tattttcagc tttcagattt tcggcaggat acatcccctc tccccaagaa
    27301 taaagatctt tgaaccaact accatttatt accttgctcc atccacaacc gaaacttttc
    27361 gagtcagtca gagattgtat tttgttaaaa tcgaagtcca gagagtgttt cttatctgta
    27421 gttattaagt tgctcagaat ttcagccaga tccttgtcaa tatatctcag tgagtattcg
    27481 ctaaatatat tttcaagctg caatatggtg tctgcatctg ctgtaggttt aaataactta
    27541 atatcagcaa g
//
