LOCUS       NZ_OW968114             4638 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 602 plasmid P3, complete sequence.
ACCESSION   NZ_OW968114
VERSION     NZ_OW968114.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065718
            Assembly: GCF_905330295.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330295.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:03:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,925
            CDSs (total)                      :: 4,807
            Genes (coding)                    :: 4,623
            CDSs (with protein)               :: 4,623
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 68 of 184
            Pseudo Genes (incomplete)         :: 120 of 184
            Pseudo Genes (internal stop)      :: 33 of 184
            Pseudo Genes (multiple problems)  :: 33 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW968114.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="602"
                     /isolation_source="Hospital Universitario La Paz (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P3"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(105..959)
                     /locus_tag="LQ120_RS24515"
                     /old_locus_tag="AI2860V1_4739"
     CDS             complement(105..959)
                     /locus_tag="LQ120_RS24515"
                     /old_locus_tag="AI2860V1_4739"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_230133888.1"
                     /translation="MLVQTCGVPQAKKQPPCGVPQAKKQPSCGEPQANNPPVASRRQTT
                     LLRRAVGKKTADAVFFISKMEPTPLKTIVYVDGFNFYYRKLKDSNKKWLNIKAMVEKLL
                     PDDEIIGINYYTARISGRIDSDAPRRQQIYFNALKSIDNLSIHYGTFSVNESRMFLVQP
                     LDFKHHLKNPNQKIKFANVLKTEEKGSDVNLGCHLVRDGFQGKYEKAVIITNDTDLAEP
                     MRVVKSELSLYIELLSPVNKPARTLSAICDKVTLISDQSIADSQFEDVIVVGEKTVTKP
                     AQW"
                     /name="NYN domain-containing protein"
     gene            complement(2008..2199)
                     /locus_tag="LQ120_RS24520"
                     /old_locus_tag="AI2860V1_4740"
     CDS             complement(2008..2199)
                     /locus_tag="LQ120_RS24520"
                     /old_locus_tag="AI2860V1_4740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2263..2586
                     /locus_tag="LQ120_RS24525"
                     /old_locus_tag="AI2860V1_4741"
     CDS             2263..2586
                     /locus_tag="LQ120_RS24525"
                     /old_locus_tag="AI2860V1_4741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001430281.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2795..>3226
                     /locus_tag="LQ120_RS24530"
                     /pseudo=""
     CDS             2795..>3226
                     /locus_tag="LQ120_RS24530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3264..3782
                     /locus_tag="LQ120_RS24535"
                     /old_locus_tag="AI2860V1_4742"
     CDS             3264..3782
                     /locus_tag="LQ120_RS24535"
                     /old_locus_tag="AI2860V1_4742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3789..4022
                     /locus_tag="LQ120_RS24540"
                     /old_locus_tag="AI2860V1_4743"
     CDS             3789..4022
                     /locus_tag="LQ120_RS24540"
                     /old_locus_tag="AI2860V1_4743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_230133889.1"
                     /translation="MTELEIYLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4386..4622
                     /locus_tag="LQ120_RS24545"
     CDS             4386..4622
                     /locus_tag="LQ120_RS24545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139242144.1"
                     /translation="MSIIIDREKYTALMTLIQTSQGYVGSKVQPNGYTLKCSFDTGFAC
                     CISRNTRNSNLSVHFENESADPATHFAILSAIS"
                     /name="hypothetical protein"
     Typing          1838..1923
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          complement(1286..1812)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="98.0"
                     /evalue="2.31e-161"
     Typing          2577..4126
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catagctata gatttcatac tctttatatt tgcctgaagg tataatgcaa aacataaaaa
       61 gcccggggga cgggcttttt ttcaacagaa cgagtatgta gccactacca ctgtgctggt
      121 tttgtcacgg ttttctcacc aactacaata acgtcttcaa actggctatc agctattgat
      181 tggtcgctta taagagtgac cttatcgcat attgcgctca gggttcttgc tggtttgttt
      241 acaggagaaa gtagttcaat ataaaggctg agctctgatt tgactactct cattggttca
      301 gccaaatctg tatcattagt tattattaca gccttctcgt atttcccttg gaaaccatct
      361 ctaaccaagt ggcaacctaa attaacgtca gaaccctttt cttctgtctt taagacgttt
      421 gcaaacttta ttttttgatt cgggtttttt aagtgatgtt tgaaatcaag aggctgcact
      481 aaaaacattc tgctttcatt caccgaaaaa gtaccataat ggatacttag gttatctatg
      541 cttttcagtg cattgaaata tatttgttgc ctacgaggtg cgtcagaatc aatccttcct
      601 gagatcctag ccgtgtaata gttaattcct attatttcat catccggtag caatttttcg
      661 accattgctt ttatgttcag ccactttttg ttggaatctt tcagcttacg ataataaaag
      721 ttaaagccat caacataaac tattgttttc aatggggttg gctccatttt agatataaaa
      781 aaaaccgcat ctgcggtttt ttttcctacg gcacgccgta ggagggttgt ttgcctgcgg
      841 ctcgccacag gagggttgtt tgcctgcggc tcgccacagg agggttgttt cttcgcctgc
      901 ggcacgccac aggggggttg tttcttcgcc tgcggcacgc cacaggtctg taccaacatt
      961 attgcatttt ttttgtctag tgcaaatgtt ttatatggtg acgaagtaag ccctacctaa
     1021 aggagaaaag atcttcttga gatccttttt ttctgcgcgt aatctcctgc tttgaaaacg
     1081 aaaaaaccac cctggcaggt ggtttttcga aggttagtta atcctggcag attctctaac
     1141 cgcggtaacg gtcttgtgcg agacatgtca ccaaatctgt cctttcagtg tagccgtagt
     1201 caggccgcca cttcaagaac tctcgataca tctctcgcac atcctgttta ccagtggccg
     1261 ttgccaatgg cgattagtcg tgtctttccg ggttggactc aagttgatag ttaccggata
     1321 agacgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
     1381 ccttcccgga gccgagtgtc aggcgtggaa tgagaaaccg cggccataac agcggaatga
     1441 caccggtaca ccgaaaggca ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1501 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt caatttttgt
     1561 gatgctcgtc aggggggcgg agcctatgga aaaacgcctg cggcgcggcc ttttccgggt
     1621 tcttgttttt tgctcacatg tttcttttgg cgttatcccc tgattctgtg gataaccgca
     1681 ttacagtctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgtagcgagt
     1741 cagtgagcga ggaagcggaa gagcgcctga tgcggcattt tctccttacg catctgtgcg
     1801 gtatttcaca ccgcatatag tgcactctca gtacaatctg cactgatgcc gcatagttaa
     1861 gccagtatac actccgctat cgctacgtga ctgggtcagg gctgcgcccc gacacccgct
     1921 aaaacctgct gacgcgccct gacgggcttg tcagctcccg gcatccgctc acagacaagc
     1981 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatccc cgaaacgtgc
     2041 gaggcagctg cggtaaagct catcggcgtg gtcgtgaagc gattcacaga tgtctgcctg
     2101 ttcatccgcg tccagttcgt tgagcttttc cagcagcgtt aatgtctggc ttctgataaa
     2161 gcgcgccatg ttaagggctg ttttttcctg tttagtcact gatgcctccg tgtaaggggg
     2221 atttctgttc atggggtaat gataccgatg aaacgcgaga ggatgctcac gatacgggtt
     2281 actgatgatg aacatgcccg gttacttgaa cgctgtgagg gtaaacaact ggcggtatgg
     2341 atgcggcggg tctgcctggg ggagccggtt gcccgttccg ggaggctgcc gacactggca
     2401 ccgccgttac tgcgtcagct ggccgccatc ggaaataacc tgaatcagac agcccgtaag
     2461 gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg tggtggccgc actgatggcc
     2521 atcggggatg agctgcgccg gctgcgtctg gccgtcaggg aacagggggc gcgggatgat
     2581 agttaaattt catgccaggg gaaaaggtgg tggcagtggt ccggttgatt acctgctggg
     2641 gagggagcgt aaccgcgaag gcgcaacggt gcttcagggt aatccggaag aagtccggga
     2701 actcatcgat gccacgccat ttgcgaagaa atacacgtcc ggtgttctgt cgttcgcgga
     2761 gaaggaactg ccgccgggag gacgtgaaaa agtgatggcg agctttgagc gtgtactgat
     2821 gcccggtctc gaaaaaaatc agtacagcat cctgtgggtg gagcaccagg acaagggacg
     2881 gcttgagctg aattttgtca ttccgaacat ggagctacag accggaaaac gcctccagcc
     2941 gtactacgac cgcgcagaca ggccgagaat tgatgcctgg cagacgctgg tgaatcacca
     3001 ttacggactg catgacccca acgccccgga gaaccgccgg acgctgaccc ttcctgataa
     3061 cctgccggaa acgaaacagg cacttgctga gagcgttaca cgcggtatag acgcacttta
     3121 ccatgccgga gagataaaag gccgtcagga tgtgattcag gcgctcacag aggcggggct
     3181 tgaagtggtc agggtgacgc gaagcagtat cagcattgca gacccgaacg gcgggaggaa
     3241 tatcaggctg aaaggagcat tttatgagca atcttttaca gacgggcgcg gagttcgaga
     3301 aaaagctgaa agagagagcc gaatctacag agaaaatgct gaacgacgag ttcaggaagc
     3361 tcggcgagtc tgtcagcgag gctgtgacct caaacgagac gaaaatcaga gacgctatag
     3421 ccctgttcac agcctcgaca gagaaatcgc tggaaaaaca ccgggaaggg gtgaaagagg
     3481 cgatgatgca gcacaggaag gacgtgttaa agctggcagg gaatacgggc atgatgttac
     3541 tggggatagt ctttctcctg tttaccgcga gtggcgggac gctctggtat cttggcggac
     3601 ggatacagac gaacctggaa gaaatcagga agcaggaaga gacattgcag aaactgaacg
     3661 cgaagacatg gggcgtggag tttgtgcagg acgggaacag gaaattcctt gtccttccgt
     3721 acgggaaatc agcggaggtg attccctttc aggggaaaga gtgggtacat ctgaaggagt
     3781 gacacagagt gacagagctg gaaatttatt tgctgaacgc cttagagcag ctgcaacagg
     3841 actatatgca gcggctgagc gaatgggaga gcgccttcgt ggaattgcag aagatgtttt
     3901 cgcttacgca acgggacaac gcgatgctga acgagcgggt catgcagttg agtcagcagg
     3961 tgcagcactt gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat
     4021 aagagaggaa cggctgatac aggagcgcga acatgtgtta tccctggaac gggagcgtca
     4081 gccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa
     4141 atggcaggtt cagtggattt tgacggacta atgtagtctg caccatcttg ttgcataggt
     4201 attcatgcgg ttaaaattta tcaggcgcga tcgcggcagt ttttcgggtg gtttgttgcc
     4261 tgtttttccg gtttcccgtc agaaacgcgc tgagggcgtc tgaggcggtg cgggcaacgg
     4321 gagttatggt aaatagattt attgctgagg tttaatgcct gcagagtaat actaatgggg
     4381 atgtgatgag tattattatt gatcgtgaaa aatatactgc tttgatgacg ttgatccaga
     4441 catctcaagg atatgttggc tcaaaagtac aaccaaatgg atatacattg aagtgttctt
     4501 tcgacactgg ttttgcatgt tgtatctcta gaaatactag aaatagtaac ttatctgttc
     4561 attttgaaaa tgaaagtgca gatcctgcaa ctcactttgc tattttaagt gctattagct
     4621 aattttttat tttctgca
//
