LOCUS       NZ_OW967982             4073 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P7, complete sequence.
ACCESSION   NZ_OW967982
VERSION     NZ_OW967982.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065712
            Assembly: GCF_905330215.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330215.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:53:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,137
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 84 of 249
            Pseudo Genes (incomplete)         :: 177 of 249
            Pseudo Genes (internal stop)      :: 46 of 249
            Pseudo Genes (multiple problems)  :: 53 of 249
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967982.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(2671..4073,1..745)
                     /gene="mobQ"
                     /locus_tag="LQ150_RS25500"
     CDS             join(2671..4073,1..745)
                     /gene="mobQ"
                     /locus_tag="LQ150_RS25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1003..1704)
                     /locus_tag="LQ150_RS25505"
                     /old_locus_tag="AI2852V1_4935"
     CDS             complement(1003..1704)
                     /locus_tag="LQ150_RS25505"
                     /old_locus_tag="AI2852V1_4935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2238..2492)
                     /gene="mobC"
                     /locus_tag="LQ150_RS25510"
                     /old_locus_tag="AI2852V1_4936"
     CDS             complement(2238..2492)
                     /gene="mobC"
                     /locus_tag="LQ150_RS25510"
                     /old_locus_tag="AI2852V1_4936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2494..2670)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2671..3029
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1004..1704)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..742
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="99.0"
                     /evalue="3.21e-157"
     Typing          2585..4071
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctgggggaac gagctggaga aataccgcac cagaatcttt gagagtgttg atttaatgcg
       61 tgacaggagt ggttatatct gtgaccgaag cgaaatcggc caacgtccgc cactgcaacg
      121 tgtttattcc tctgcaacct ttgagcaact gctgacgcag gtttgccaga catggccgca
      181 acatacccgc aatctccgcc agccgaagac atggccggag tctttctgtc ttggagaaga
      241 ccgccagcca gccatgccat caccagcggc aaggaaggtg gattttatgc agggacgtct
      301 gccaccgacg ctgatgccgg tcatgagttc tgtcgaccgg gaaacccgcc agcttcagct
      361 tttgctggtc atgggcgtgg atgactctct gggcggtgtt atccggctta atggtacgct
      421 gtatcccgcg tttgccgttc cctcagccga taacaggcat ctggtcatca gtgcgctgac
      481 cgataagggg ttacggtatg cagggtatgg tgttgccgta aatcacgatg cagacagcca
      541 catatcacct gcaccagaac tgatgcagtt tcatctgaaa acccgtgagg caccgctttt
      601 tgctgccgtc aacacaccag aaaaacagcc ggatcatctg ttccgctcgc tgggttttaa
      661 tcgtacctgg gaggaatggc agcgcgagga agatgcacga acacacacca ccgagaggag
      721 acatgatcgc ggttggtcac agtgaatgtg aacaatattt tgttttgttt aacatcgtgt
      781 tatgttcgtc gatgttcttc gatgttcatc gacagggatg aatgcaagaa gcacactcac
      841 ataacgcaag catagttttg tttttccgcc cccaaaaatt ggggggtctt ttttcgctgc
      901 atcagagcgt ttttgacgca atttttcact tcaatagtac cttcgcacta ctaacacaaa
      961 aaaaacgcag cagggagcgt tttaggtgcc ttaaaatcga ttttagatcg tcatcttgat
     1021 ctgtttgtct tccttgaagc gaaaatgcag ggctacaacc gtgcgcccct ttttgatcgt
     1081 ctcaacagtt acaaccagat cgcttttttc gttcagttcg gcgatggctg gcttgattat
     1141 catcttattc aagtctttga attgttgata tttatcttca acggcaagca tggttcggaa
     1201 atcatcgata gctatcgttc tgtcaccggt gctgcggaac tgctggagca gctcgtaaat
     1261 gcggatgctg taggtgctgg aaaggctgga gacgttcttc acgactaccc gcgtaaactg
     1321 ccccttgagc tgtgtcagat acggcatgat ggcatcggag aaggtgatat ctactttcgc
     1381 ctcacctttg aaatactgcg cccggtactg cacccagcgg aactcttctc gtttttcctc
     1441 gttcttgatg attactgacc tgtcccagag gcgatctatc gcttccttga gcctagcttc
     1501 agcgtgctgc cgtcccatgt caggaaaggc atcatgaaac tctgcagccg tgatggtcat
     1561 ggttttttgt tcatccggcg aaggggaatc ttctccggat tttaggcggc tgatacagac
     1621 cagcaggaag cgctgttcct gcaaagtgag cttgtagctt gcctcgataa gggcgtttga
     1681 tttgtaagct atcagatctg acatttcgct tgctcgttgc tattggtggc gtttttttac
     1741 tgtacgccac ttatcatatg ggtggaagtt ttttttttgc aacccccttt ttctgtggat
     1801 tacaccccca aaagctccac ctttcacccc caaaagctcc acctttcacc cccaaaagct
     1861 ccacctttca cccccaaaag ctccacttat cttgccagaa catctttaaa atcatggtgt
     1921 tacaagacct aaaaaggttt taaaaaaaaa gatctaaaaa taagatctta aaaacttaac
     1981 ggcgagcctg tggataactt ggggtgctga aaacaaaaaa ccaataaaaa caatgcgttg
     2041 caataaagca gaattgcaag tttcgaatac accccctcca accctgtgga aacccagtat
     2101 tcatgcgggt tcagcgcata tcacctgccg aaaaaccgtg cgtatgtgct caacctagcg
     2161 actgattcac ttccgcttcc tcgcccactc gctcgctaac gcgcggtcgc tggggctgcg
     2221 gcgagcggac gatacaatca ctgttctggc agcgcctcca gctcgttaat cagcggcagt
     2281 atgcgcgtca catcagcctg tcgtgtcacc tctgccttca ggaactggag taaccaggca
     2341 cgcaactgca cctcctccgg ctttctgacc tttgccagca atgcagcacc cacaacaacc
     2401 ttctgcgctg tgtccaggct ccggctcttc gccttcaggc gctgtaatct cgcctcagca
     2461 tcagcaatct tctgttcgag cgttctgctc atgatgtaac tccgtgcgcg atgaaaaatc
     2521 gcattttagc gcgtcactgg tagtttaaaa actgagctgg cataatgcac ggcaaatcac
     2581 gaagtgcgca cttatacaat ctccactccg tttcgattgt gtgcgccctg cggggctgaa
     2641 agaaaactac aaaaaggcat tacggcagaa atggcgattt atcatctcag catgaaaatt
     2701 atctccagaa gcagcggcta cagcgccgta gcttccgctg cctatcgttc cggttcactg
     2761 atgcttgatg agcgtaccgg actgactcac gactacaccc gcaaaagcgg tgtggctgag
     2821 gctgtgattc tgacccccgc caccgcccct gcctggtgta cgaaccgtgc cgaactctgg
     2881 aatgccgttg agaaggccga acgccggaag aactcacaac ttgcgcggga aattgaactc
     2941 gccatccccc acgaactgcc gcaggatgcc gcacgggaaa ccgttctcgc ctttgtcaga
     3001 gaaaactttg tcagtcaggg catgattgct gatgtggcgt tccatcacat ggataaaaca
     3061 aatccccatg cgcacatcat gctgaccacg agggctatcg gacctgctgg gttcagcgga
     3121 aaggtcaggg actggaacga ccggacacat gccgagacgt ggcgggcatc atgggctgac
     3181 catgcaaaca gggcactggc gaacgccggt tatcaggaag agatagacca tcgttcatac
     3241 gagcgacagg ggcttgagaa aacgcctggt attcacctcg gaaagtctgc ctgtgcgatg
     3301 gaaacacgcg gtatcgaaac agaacgcggc gaacagaatc gtctgattaa caggcttaac
     3361 ctggaaatac agatttcccg caccgaactc aggaacggag ggctgacaga gcctgcccgc
     3421 cgtgtggctg accttctgaa tatcgggctt cctgacaacg caacttcgta cacactacgt
     3481 gacattatcg aagcgttgcc gaaggacagt aactcggcat ggaaactcac cagtaatgcc
     3541 atgtcgatga tggcagatat gcaggcgacg cgcagacgct gggaagagct gaacaccgaa
     3601 cgaaaggaag cgatgagcca tgctgccagc ctgaaaaaat catcgccgtt ttcgtccggt
     3661 ttttcccgta ttcccctgat gcaatgggcg gcaccggaat accgtagtga gcagggaaaa
     3721 atccagtctc tgagccagca aatggaaaaa ttacgacagc attaccggaa ggtggagaaa
     3781 caggacattc ccgcgagtca gatggcattt gaaaaacagt ggcagcagtg gggggaatcc
     3841 ggccttgcag ctctcagagc ccagttgctt aaacgtgaag aggaattgcg ccggaaggaa
     3901 caggaagata ctgaacgtag tcgcgcggag cagctcagga aaaatgacaa tgccgttatg
     3961 gattcagggg tgctgagagc agtcgttacg gggtatggcg aggctcccat gcctggtaac
     4021 ggggaaatga cctgttacct tctgcttcac aaccggaacg gagaatatac gct
//
