LOCUS       NZ_OW967980            12602 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Escherichia coli isolate 10 plasmid P5, complete sequence.
ACCESSION   NZ_OW967980
VERSION     NZ_OW967980.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065712
            Assembly: GCF_905330215.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330215.2-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 03:53:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,137
            CDSs (total)                      :: 5,016
            Genes (coding)                    :: 4,767
            CDSs (with protein)               :: 4,767
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 249
            CDSs (without protein)            :: 249
            Pseudo Genes (ambiguous residues) :: 0 of 249
            Pseudo Genes (frameshifted)       :: 84 of 249
            Pseudo Genes (incomplete)         :: 177 of 249
            Pseudo Genes (internal stop)      :: 46 of 249
            Pseudo Genes (multiple problems)  :: 53 of 249
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967980.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="10"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(12574..12602,1..955)
                     /locus_tag="LQ150_RS25395"
                     /old_locus_tag="AI2852V1_4912"
     CDS             join(12574..12602,1..955)
                     /locus_tag="LQ150_RS25395"
                     /old_locus_tag="AI2852V1_4912"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(1414..2111)
                     /locus_tag="LQ150_RS25400"
                     /old_locus_tag="AI2852V1_REPA000000288"
     CDS             complement(join(1414..1862,1862..2111))
                     /locus_tag="LQ150_RS25400"
                     /old_locus_tag="AI2852V1_REPA000000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            2270..3814
                     /locus_tag="LQ150_RS25405"
                     /old_locus_tag="AI2852V1_4914"
     CDS             2270..3814
                     /locus_tag="LQ150_RS25405"
                     /old_locus_tag="AI2852V1_4914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<3907..4171)
                     /locus_tag="LQ150_RS25410"
                     /old_locus_tag="AI2852V1_REPA000000285"
                     /pseudo=""
     CDS             complement(<3907..4171)
                     /locus_tag="LQ150_RS25410"
                     /old_locus_tag="AI2852V1_REPA000000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(4316..5192)
                     /locus_tag="LQ150_RS25415"
                     /pseudo=""
     CDS             complement(4316..5192)
                     /locus_tag="LQ150_RS25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(5391..5675)
                     /gene="nadS"
                     /locus_tag="LQ150_RS25420"
                     /old_locus_tag="AI2852V1_4916"
     CDS             complement(5391..5675)
                     /gene="nadS"
                     /locus_tag="LQ150_RS25420"
                     /old_locus_tag="AI2852V1_4916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(5677..6009)
                     /locus_tag="LQ150_RS25425"
                     /old_locus_tag="AI2852V1_4917"
     CDS             complement(5677..6009)
                     /locus_tag="LQ150_RS25425"
                     /old_locus_tag="AI2852V1_4917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6154..6390)
                     /locus_tag="LQ150_RS25430"
                     /old_locus_tag="AI2852V1_4918"
     CDS             complement(6154..6390)
                     /locus_tag="LQ150_RS25430"
                     /old_locus_tag="AI2852V1_4918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            6735..7103
                     /locus_tag="LQ150_RS25435"
                     /old_locus_tag="AI2852V1_4919"
     CDS             6735..7103
                     /locus_tag="LQ150_RS25435"
                     /old_locus_tag="AI2852V1_4919"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(7533..7667)
                     /locus_tag="LQ150_RS25440"
     CDS             complement(7533..7667)
                     /locus_tag="LQ150_RS25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(8851..9168)
                     /locus_tag="LQ150_RS25445"
                     /old_locus_tag="AI2852V1_4920"
     CDS             complement(8851..9168)
                     /locus_tag="LQ150_RS25445"
                     /old_locus_tag="AI2852V1_4920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            10253..10402
                     /locus_tag="LQ150_RS25450"
                     /old_locus_tag="AI2852V1_4922"
     CDS             10253..10402
                     /locus_tag="LQ150_RS25450"
                     /old_locus_tag="AI2852V1_4922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            10862..12090
                     /locus_tag="LQ150_RS25455"
                     /old_locus_tag="AI2852V1_REPA000000290"
     CDS             join(10862..11177,11177..12090)
                     /locus_tag="LQ150_RS25455"
                     /old_locus_tag="AI2852V1_REPA000000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            12121..12489
                     /locus_tag="LQ150_RS25460"
                     /old_locus_tag="AI2852V1_4925"
     CDS             12121..12489
                     /locus_tag="LQ150_RS25460"
                     /old_locus_tag="AI2852V1_4925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     Typing          10045..10218
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(7560..7925)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(9570..10078)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgtatataac gttgaaaaat acttaaggga atgtattcaa tcagtattag atcaggattt
       61 taacgactat gagataatct gtgtaaatga tggttcaaca gatggcagtc tagatatcat
      121 actgtcattt gatgaccaaa gaatcaaagt aataaatcag gaaaataaag gcctgtcggt
      181 ggcaagaaat gtcgccattg aatcatcaac tggtgaatat atatggtttt tagatagtga
      241 tgataaaata tcacatggaa gtctaaagtt aatagacaag ataaccaatg aaaaaaatta
      301 tgggattatt ttctttaata cacatgtttt ttttgacact ccagaactga aaaaaaaagg
      361 ctggcatgat tataagcgtt caacatttgg tgaaatggat gcattaactt tctttgaaaa
      421 agaaatgacg aacaaaaaac taatagtgtc agcatgctgc tatgttttta gaagggaatt
      481 attaaatggc aatgcattca ttccgcatat atatcatgag gataatcact ttatagcaac
      541 cctgattttt gacaattatt caactatatg cctctcgttg gatgaggcgt tatatcttag
      601 gagggtaaga aatgattcca taatgacgtc taaaaaatca atgaggcaca tcattggata
      661 ttcaacatgc atcgatgagc ttacgaagta taaaaaaaca aaaggcatac aaaacaagag
      721 ctttgataga tttgtgaatg gaatggtttt gtcgttattc tctatagcgg aaacaataaa
      781 aacaaaggaa gcaatgaact atgtggcgag atataaaaaa caccccgacc tatcatttaa
      841 agatagagca ttaattaatt acccattaat taaaaagact tatttaaaac taaaaaacgg
      901 gaaggtgttt aattacctaa ggaatggcat tattaataaa aaaagcaacc cttaataaaa
      961 gcaaagcgat aattaataaa ggtttttctt tgttgaataa ataagatttc ggataggtat
     1021 ccccgtgacg ggttgggttt tcaggcgata cgcacgcttt ccggggcttt gttgaataaa
     1081 tcgaactttt gctgagttga aggatcagat cggggtcagt ttggagatcg gaaatttttg
     1141 tacgttaagc gcattattta accaatcttg gttcgtaagg ggttgatttt gcgtcagtgg
     1201 ctatcgcttt taagttgtcg ctcttgcagt atcgcattta ggttatccga ttaaggtcaa
     1261 acctctgaaa atgccgtata gcgcgggagt acaccctatt gctataggta agtctgttca
     1321 aaaaacaggc ttaccgtaca ataattctct atatccaaac taaccccaga tcacgcatct
     1381 tcccgacaac gcagaccggg taatgactcc aacttactga tagtgtttta tgttcagata
     1441 atgcccgatg accttgtcat gcagctccac cgattttgag aacgacagtg acttccgtcc
     1501 cagccttgcc agatgttgtc tcagattcag attatgtcgc tcaatgcgct gcgtatatcg
     1561 cttgctgata acgtgcagct ttcccttcag gcgtgattca tacagcggcc agccatccgt
     1621 catccatacc acgacctcaa aggccgacag caggctcaga agacgctcca gtgtggccag
     1681 agtgcgttca ccgaagacgt gcgccacaac cgtcctccgt atcctgtcat acgcgtaaaa
     1741 cagccagcgc tgacgtgatt tagcaccgac gtagccccaa tgttcgtcca tttcagcgca
     1801 gacaatcaca tcacttcccg gttgtatgcg cgaggttacc gactgcggcc tgagtttttt
     1861 aagtgacgta aaaccgtgtt gaggccaacg cccataatgc gtgcactggc gcgacatccg
     1921 acgccattca tggccatatc aatgattttc tggtgcgtac cgggctgaga ggcggtgtaa
     1981 gtgaactgta gttgccatgt tttacggcaa tgagagcaga gatagcgctg atgtccggca
     2041 gtgcttttgc cgttacgcac cacgccttca gtagcggagc aggaaggaca tctgatggaa
     2101 atggaagcca cgcaagcacc ttaaaatcac catcatacac taaatcagta agttggcagc
     2161 attacccact attggtggag atgatcataa tgccttataa tcatacggga tacgaactta
     2221 taaagtccta aagtttagct aaatcggaat ctacaacgta gatgatagct tgaagaatta
     2281 tgatgtcttt tatggtggta tctttaaaac catcacggat tataaattat tgaaaattca
     2341 aaaggatatt aaaaacaata tgactgcact agtcagtagt tttaaatggg ttggcattgg
     2401 acaacttgta aaaatagcaa cacaattggt ttcgttattc tacttgacgc acttaatccc
     2461 cccatcgagc tatggactat tagcaatgtc atctgtggtt atgaatttag caagcatatt
     2521 caatgacatg ggaactgggg cggcagtaat tcagcgacaa gaggcgtcga tcggttttta
     2581 taattacata tacaggataa acatttttac tgggttgttt gttatgctgc ttgtaataat
     2641 aacatcccca ttaattgtcg tgtactttaa cacacccgag ctatacccgg ttctatttct
     2701 attggctttt agctttccaa tttccagctt aacaataact cacaaagcaa aattagaaaa
     2761 agaaatgaac tttaaagtca tcattgcaat tgaaattttc tctagcctta caggaatggt
     2821 gctggcaata gttttagcta atttaggctt tggtgtttat agtttgattt ttcaaacact
     2881 gacaacaata tcaatatcca caatatgttt tttgaaggtt tccgggttga gtttatcatt
     2941 gagaattaaa aatatcagcc atagtgacaa gtcaggagtt atagggttta gtggttattt
     3001 attgtctttt aatttaataa attatttttc caggaatctt gattctattt taataggccg
     3061 ttttttctca tccgcaatac ttggggtcta ttcgattgcc taccgcatta tgttatttcc
     3121 gttgcagagc ctgacgcttg ttttttctag agttttcttg ccacacctta gcaaaaacat
     3181 aaataacact aataacacta ataaacaaga ttacttaaat gcattaaaac ttatactatc
     3241 attaagcgcg ccactgatgc taggtttatc tgctacatct ttttctttta cgaggatttt
     3301 ctttgatcct aagtggcacc tacttggaga cttattgcat tggttggcac ctacagcgat
     3361 tatccaatca gccttgagta caacaggttc tgtttttatg gcctacggaa aaacaaggtg
     3421 gttgtttttc ttaggttgca ttggcgcttt tttgatgggg ttgtctttca ttataggtgt
     3481 tcatttcgat ataaaaacat tggtctacct ttattttttt gcaaacttaa ttaattcact
     3541 cccggcattt attttactta agaatatctt gaagtttagt tacattgaat tggtaaacgt
     3601 gttttttaga actatctccc cagctttttt gatgttctta cttattaaag tatggcagca
     3661 tttttccttt ttcaaaaatg ccagcattga tttttctttt aatgtcgcat tgggtatttt
     3721 tagttatatt gttttttata ccttgtttaa tttcaatgag gttgttagga caggagtaat
     3781 tcataagtta agatgcatga ccaagttaaa gtgattcatt tattaaattc attaaatgaa
     3841 ttggtaatga ctccaactta ctgcttgtgt tttatgttca gataatgccc gataccgact
     3901 tcggcctaat ttttttaagt gtcgtaaaat cgtgttgagg acagtgccca taatgcggga
     3961 tgtggcccgg catccaacgc cattcatggc catatcaatg attttctggt gtgtaccggg
     4021 ctcagaggcg gtgtaagtga actgtagttg cttgctatgt tttacgacag tgagagcaga
     4081 gatagcgttg atgtccggta gtgcttttac cgttacgcac cacgccttca gtagctgaac
     4141 aggagggata actgatggaa atggaagcca cgggagcacc tcaaaaacac cattagacac
     4201 taaaccagca agttggcatc atcacaaaca gttgttactg ctggaaaaaa aacactatca
     4261 acacttaagt aatggtgatt caataattaa gattcagtat gtaaaatact gaatcttaat
     4321 tataaatttt cctttttcta aaaaatgtag cgcgactaat acctaaagca acccaaggct
     4381 cccctagaga cagcctacct cctcctgatg atttttcacc accaactttt cctcttttgg
     4441 cttgtataag tgaaaaattt tgcttattaa atttcaaaaa tgtccacttg cagatacttt
     4501 tcgctattcc cgttacttca ttctcattca aaggcactgc aaattgcaga ttgtaagcgc
     4561 aggcgcgttc atggcaggct tgtagccact gttcgtactc aggccagccc tgacggatag
     4621 cgcgatatgc ccactttctg gttttatcaa acagtatgca gttacgacct aagccataat
     4681 cggcgacgat ttttttatcg ttcgcagcgt tcaggtcgag tgattcggct aaccagtcca
     4741 gcgtgtagag ttcaggctgc catacgggca attttccagt gactgtggtt cggattcttg
     4801 caaattagcc ccgaataccc cgtgtcagcg cccaattttt tacgtagcgc gctctctaca
     4861 gcagcggcgt aacgtaacgg tttcattttt ccgtctggcg cggtgcgtat cgaggttttc
     4921 agtgcgtaca gcaggtgcgc gtgtccgttt tcggggttgg tgatggtcag tgtcggcgcg
     4981 ggggcgttgc ggtcgctcca gtcaatggct gcgccgatgc ggtcaacgtc aaatcccagc
     5041 cagaacatgg cgtgaggctg attaaactgg atgtatttag ccagaatagc gcgctctttc
     5101 cctgcgatgc ggacaccaaa gtgtaaatca tccgagaagt acggtttatg cggcaagcgg
     5161 tcgttgaaaa gttcgagtgc tgcgttacgc aatgtgccct gaacttatgg cgggctgttg
     5221 tctggcacgg ttcatgtgct aatatccttc ttaggtttta tacctagccc tgatttatca
     5281 tgtctcttcc gccaagttga aatcatgatt atcggggctt tgttttttct gttgccgtta
     5341 aagttacacg cgaacggcct cgcctgtcga atttcttctg tcacatcagc ttaatgcgcc
     5401 gccagttcat caaaaaactt tgggttagcc tgaatggtgg ccagcacttt tgctgccagt
     5461 ccggttgggt tacggcgtcc ggtttcccag cttttgatgg tatcgaggct ggttcccagc
     5521 actttcgcca tttcgctctg agtaatattc aggttttctc tgatagccct gacgtctgcc
     5581 agctcgtagc gggtcacacg ggcgggggct ttggttccgt ttttgatttc aacggcctct
     5641 tccagagagg ctttcagctc gtcaaaaaaa ctcatcttac acctcgtctt tcagtcgttg
     5701 ggtcagcttc ttcagttctg ctttctctgc gtctgtgatg ctgtctttgg tgcttttagg
     5761 gtaggccatc accagataaa tcacctctgc ggtggcaagg aagtaaatca cccgcgcact
     5821 gccgctcttt ccctgattcc ccgtcgccat cctgatcttt cgcagaccgc ccgttttctg
     5881 gatgaggtca cctttgtccg gtgactcgat aagctctttc tgaagctctt tgatctcatc
     5941 atccgtggcg atttgcttta tctgacgggt aaacatcgtt gtctcgataa actcaatcgc
     6001 gtgactcacg gctgtctcct gtatattttg atgtacaatg tacaccaaga ggcgtgttct
     6061 gaaaagccgc gttctgtgcc atcagacgtt acatggactg acctcgatag cgggttgctt
     6121 gcttctcctg ctgtaactgc cgttcctgct gctttacgag cgtgttaatc ggctcagatc
     6181 gctgctcaag cgttcgacct gttcgtgcaa ggcgttcacc tgattgctca agttcagaca
     6241 taccctgtcg ttgcgctcca gtgcctgtga ggtagtctcg aacatgcgtt gcaagctgct
     6301 gtaactgtcc tgccacgcct gcttctgctg ttcgtagctt gtttgtaagc tttctaaggc
     6361 gctcagaagc tgcctttcca tctcggtcat ggtttttcct gtcctcagtg ataaaaacgt
     6421 gctgtggcgt ttctgtggtg tctgacgggg ttatttcgcg gtgtacttca tcacggtgtt
     6481 tccggctgtc gatgtgtatg tctcggtgcc atgcgctttc ttcggcagta ccaccagata
     6541 ggtgttcttc tgcttgtcgt ccttgtaaat cgctacccct gcgccgctct ggggtccgct
     6601 tggaaaaagg aaattatcct acgctaagcg cattttttgc acagatggct accgactgaa
     6661 cgtaacgtgt gagtcatgat cagaaatgtg ttaactctag gaattgacac gacttactgt
     6721 ttgatgggga aatcatgttt agctatctca aggctatgta tcatcaatca aaaattcagg
     6781 ctgaactcaa ggcacagata catgaacaaa ctacggttaa tgctatctgt catcaccctg
     6841 aatcaatcga gatcattgcg gtttgttcga ctgatgcgta ttaccgaaaa cgtaaggacg
     6901 ctgcttttct gacaacctgt tctgtgctca tgcgtacttt aaaagatgaa tccgtaccga
     6961 tggtactacg gaaaacagca tggcgattat tgaacgaaag ataccaacga attaagttaa
     7021 atcaggctta taggatagaa aatttcttgc ttgttgccga ttttgaatat gctctcgaag
     7081 aacatgatga actagcagag tagtactagt gaggcgatga ataccgacag ttaacgtgca
     7141 tgtgacttaa acgtccgatt acgtacagca tgtagacaac atgtccgaaa gtaagcaaaa
     7201 ccattaacgg acagccggat catttcgtac atcccttacg gtcaggggtt cgcgctcaat
     7261 ggaacgaaat cacccgttag cttaaaacgc ttaacgtagg ataatttcct ttttccaagc
     7321 ggacccgcaa tacagcaagg aacttcttga gatcctttct gttgcgtcgt aatctcttgc
     7381 tctgtaaacg aaaaaaccac cctggcaggt ggtttttcga aggttagcta atcctggcag
     7441 attttctaac cgcggtaaca gtcttgtgcg agacatgtca ccaaatctgt cctttcagtg
     7501 tagccgcact caggccatca cttcaagaac tcttactgca tctctcgcac atcctgttta
     7561 ccaatggccg ctgccagtgg cgtttttgcg tgtctttccg gattggactc aagacgatag
     7621 ttaccggaca agacgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     7681 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa
     7741 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc
     7801 agggggaaac gcctgggatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     7861 tcagattctg tgatgcttgt caggggggcg gagcctatgg aaaaccggca gcgccgcggc
     7921 ctttcctccc tcgctttatc tcgtcttcat cgccatatgc ctttgtgaat attctccgtc
     7981 cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatct
     8041 tcggcctcac gtccggcacc ttacactccg taactgcatt ttttcatagg atgcaggata
     8101 gggctaacgc cgcaaagtga cgtcacctga cgttcctgga ttacaaaggt taaagcagcg
     8161 gacaggaaat gttttgtgcc cacagctatg ctgggttatt cacaaaacag cgggacaaaa
     8221 tgtgtttttg gagtaccgcc ggcccgctaa agactcctgg ttagctcccg catgtttgct
     8281 atctttttgc taccattttg ctactttttt gctatctcca gagtcttctg gtctttcggt
     8341 tgctatcctt ttgctacttt tttgctatca aaatgctacc tctcccttct tgcaataaat
     8401 gaccaaggca cgttagcgat gtatacatcg ccgcgccgca agggtgcccc ttccgaatcc
     8461 cgttcatcac tgtgtgctga ttaatccagg ttaaatccga cccccttagc aataaatcct
     8521 gccatgcctg acggcctggc caacgcctca cggtcgcaca gtaaactgcg caccctttca
     8581 ccgttatcgg tggggttcgt ggtggctttc agggcgctcg ccggatttta gccgctaaaa
     8641 tgacgtccat gcgttcgtgt gcatagccat gcagacgcga agaaatcatc aggcgcgttt
     8701 tttcacgata tggacaggga aagatgccgg aaataggcag gagggcgcag aaatgccctg
     8761 agggcgtctg agatggtgca cgcaacggat gttatggtaa attcagctga ttggcaggta
     8821 caaaaaaagc cccgagggct tttttgtacc ttattgatct gcacactttg cagccttcag
     8881 ggcttccagc tgatttagcc ggttagctgc ttttttgcgg gcttcgcctt ttgccatgcc
     8941 atttcctatc ccaaaatcac ccaggaaacc taatacggtg cgcccgtcaa attcgccggt
     9001 ttcctcaatt tcttgttgta cactgcgcgt tttggcgatt tccagagcta ccgctttgca
     9061 atccattgcg gaagattctg gaccggtgac gttcacggcc tgtggatatt gctttgttgc
     9121 gcatcctgcc atgatcagcg ctgccgatag gatgaatatt tttttcaaaa ttatccttat
     9181 ttatttaggc atagttaaga gatacccatt ttatcggcag gaaatgaggg ttttttagtt
     9241 gaattttggg aggggcaagg ttccatattg gtcgaggatt ctggagctga ccggcaacgg
     9301 ccagcgcggc tatgggccat gcggtggttg agcatagaaa ggatcttctt gagttccttt
     9361 ttttctacgc gtaatctctt gctctgaaaa cgaaaaaacc acctggggag gtggtttttc
     9421 gaaggttaag tcagttgggg aactgcttaa cctggtaact ggcttcagca gagcgcagat
     9481 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctatagc
     9541 accacctaca ttaactcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgctt
     9601 tgtcgtgtct ttccgggttg gactcaagac gatagttacc ggataaggcg cagcagtcgg
     9661 actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaactga
     9721 gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga
     9781 aaggcaggaa caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat
     9841 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg
     9901 gggcggagcc tatggaaaaa cggctttgcc gcggccctat cgcttccctg ttaagtattt
     9961 ccctggcatc ttccaggaca tctccgcccc gttcgtaagc catttccgct cgccgcagtc
    10021 gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat
    10081 tctgctgacg caccggtgcc gccttttttg tcctgccaca tgaagcactt cactgactcc
    10141 ctcatcagtg ccaacatagt aagccagtat acactccgct agcgctacgt gactggttca
    10201 gggctgcgcc ccgaaaccct ctaacaacct ctcagctact ggatatcacc ccatgccatc
    10261 tgaacatgat gaacaactgg attcgactga tacgcagctt cccggcgaat atgacctttt
    10321 tgatttaatg gcgcaggtta ctgaagagaa cctgcatgcc ctggtggatt ttgggccgcc
    10381 tgtcggccgg gaagcgttat agctttttcg cttacccgcg tacgtggtaa tacgccggta
    10441 ttacatctta tttctttatt ttcatgtaat tacaggtggt tgtaatacgt tgcgtattac
    10501 ataaataaac ggggacttca gtttttatct tttaattcat gcaattgcga tgaaaatcgc
    10561 ggttgcgtat ggtgtatgac catttaaggg ataatgtaac ctgactttta aagaatgaat
    10621 ctatctttgg cagcgcatga gatcgataaa aagcgttcca catcaggcgg ttcatgtatc
    10681 catgaaccgg aaaggacaaa aataagacaa ttgtaagaca ttttttgcgt tttactctta
    10741 ttttggacta ttatgagaca aatgtaagac tagactggcc ccctgaatct ccagacaacc
    10801 agtatcactt aaataagtga tagtcttaat actagttttt agactagtca ttggagaaca
    10861 gatgattgat gtcttagggc cggagaaacg cagacggcgt accacacagg aaaagatcgc
    10921 aattgttcag cagagctttg aaccggggat gacggtctcc ctcgttgccc ggcaacatgg
    10981 tgtagcagcc agccagttat ttctctggcg taagcaatac caggaaggaa gtcttactgc
    11041 tgtcgccgcc ggagaacagg ttgttcctgc ctctgaactt gctgccgcca tgaagcagat
    11101 taaagaactc cagcgcctgc tcggcaagaa aacgatggaa aatgaactcc tcaaagaagc
    11161 cgttgaatat ggacgggcaa aaaagtggat agcgcacgcg cccttattgc ccggggatgg
    11221 ggagtaagct tagtcagccg ttgtctccgg gtgtcgcgtg cgcagttgca cgtcattctc
    11281 agacgaaccg atgactggat ggatggccgc cgcagtcgtc acactgatga tacggatgtg
    11341 cttctccgta tacaccatgt tatcggagag ctgcccacgt atggttatcg tcgggtatgg
    11401 gcgctgcttc gcagacaggc agaacttgat ggtatgcctg cgatcaatgc caaacgtgtt
    11461 taccggatca tgcgccagaa tgcgctgttg cttgagcgaa aacctgctgt accgccatcg
    11521 aaacgggcac atacaggcag agtggccgtg aaagaaagca atcagcgatg gtgctctgac
    11581 gggttcgagt tctgctgtga taacggagag agactgcgtg tcacgttcgc gctggactgc
    11641 tgtgatcgtg aggcactgca ctgggcggtc actaccggcg gcttcaacag tgaaacagta
    11701 caggacgtca tgctgggagc ggtggaacgc cgcttcggca acgatcttcc gtcgtctcca
    11761 gtggagtggc tgacggataa tggttcatgc taccgggcta atgaaacacg ccagttcgcc
    11821 cggatgttgg gacttgaacc gaagaacacg gcggtgcgga gtccggagag taacggaata
    11881 gcagagagct tcgtgaaaac gataaagcgt gactacatca gtatcatgcc caaaccagac
    11941 gggttaacgg cagcaaagaa ccttgcagag gcgttcgagc attataacga atggcatccg
    12001 catagtgcgc tgggttatcg ctcgccacgg gaatatctgc ggcagcgggc ttgtaatggg
    12061 ttaagtgata acagatgtct ggaaatatag gggcaaatcc aaagacatta tgggcggtat
    12121 atggcgaatt ttaaccctgg tccaagaaca gccattgcag ctgtcaggga ttccaagaac
    12181 gatatagaaa cctggaagaa aaatggtatt cgtagggaag agattgtgac tgtgctgaat
    12241 gaacgttacg gcctgaatat ctcactggtt gcgtttgata aggctcttta cagattgaga
    12301 aaaagtacag atgaaccgtc tcttgctgat gtggattctg tgtctgctgc agaaacgaat
    12361 ttctctgaag ccagtgggcc tgacaatggt ttttttctgt ccgggaatga cttcatgcga
    12421 gcagaagaag aatctcaaag agtggttgag tcattcagag atagccttag caggaagaaa
    12481 ggattctgat ttattcaaca aagccgttga taaatcgcat tccataaata acaacttgat
    12541 gttagtataa ctaaatattg tttggagatt actgtgaaaa aaaaattttc gataatagta
    12601 cc
//
