LOCUS       NZ_OW967972             2924 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 648 plasmid P6, complete sequence.
ACCESSION   NZ_OW967972
VERSION     NZ_OW967972.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065716
            Assembly: GCF_905330275.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330275.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:25:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,224
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,875
            CDSs (with protein)               :: 4,875
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 71 of 233
            Pseudo Genes (incomplete)         :: 168 of 233
            Pseudo Genes (internal stop)      :: 39 of 233
            Pseudo Genes (multiple problems)  :: 40 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="648"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P6"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(2491..2924,1..58)
                     /locus_tag="LQ127_RS25955"
     CDS             join(2491..2924,1..58)
                     /locus_tag="LQ127_RS25955"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_063660750.1"
                     /translation="MLKEHAKIDNVLDFDLSLNTVEEINKMQEKGDVKVTTIVQNEKKV
                     SLKDQFVMAFTSNLRALAELNISQNELKVITYILEVMEFGNLISINQTSIAKELGLHKS
                     NVSYTFKRLVEKNILIKKDGHTFMNSNLFAKGLNHSMNEERRANLRSAQVENEHFKRSF
                     "
                     /name="replication/maintenance protein RepL"
     gene            complement(343..1494)
                     /gene="mobV"
                     /locus_tag="LQ127_RS25960"
                     /old_locus_tag="AI2858V1_5028"
     CDS             complement(343..1494)
                     /gene="mobV"
                     /locus_tag="LQ127_RS25960"
                     /old_locus_tag="AI2858V1_5028"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_096216837.1"
                     /translation="MYAICRVGKIKSQSQLSQAQSHNLRQRETFNADPLKTSNNEILYG
                     SPFVRDDVNKRLEESGVKIRKDSVLCAEMILTASPEFFNTDNSFKEWKNENLNFLKNKY
                     GENLINVIIHNDEKTPHLHAIITPIISDKNNNRLSMKEFLGGKKLLSELQTDYAKSMEK
                     FGLSRGIEKSVAKHQDIKTFYSSIEDAKEIGQETKEEFKKDFKVAINIKTPTPTGRIFK
                     TITPEEAKKSTIENLQEYKKKVNEKIHPMHDGIVNLSIENKRLQTENTQLREVAARLNR
                     DNEISRKRDQKFDHMRKLCPDEYNQLQKAYKNRLNEIEQKKKQDEELKLKQKLELEQKR
                     KEELAKRPKGESSLDLDEQRKNKMKPEPEPQQQIEYSRPRPRF"
                     /name="mobV"
     gene            complement(1497..1649)
                     /locus_tag="LQ127_RS25965"
                     /old_locus_tag="AI2858V1_5029"
     CDS             complement(1497..1649)
                     /locus_tag="LQ127_RS25965"
                     /old_locus_tag="AI2858V1_5029"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161653071.1"
                     /translation="MVIVIVLEMLFGFMLMGWIISSCISTKDKSQEIRNSYSEISKLKK
                     KIKGR"
                     /name="hypothetical protein"
ORIGIN
        1 aagagcgaat ctacgatccg ctcaagttga aaatgaacat ttcaaaagaa gcttttaaaa
       61 ataaaactca atacgcaatt tgattataac aataaaaaaa gggattttgc aaatcccttt
      121 taatttatat aaattaaaag ctccgatcta cggagcttaa ttatttttga aaaatccatc
      181 attgccagca gctccgcagc aggcggctac gccctttata tttattatta tggttcgggt
      241 cgcttagaca agttcttctt gtctaagtgg gggtgggggt gagcaatcta tctgcgagat
      301 tacgcagata gtgcgagggg gaacgggttt aggggttccc ccttaaaatc tgggtcttgg
      361 tcttgaatat tctatttgtt gctgtggttc aggttctggc ttcattttgt ttttacgttg
      421 ttcatcaaga tcaagactac tttcaccttt tggtcttttt gctaattctt cttttctttt
      481 ttgctctaac tctaattttt gttttaattt taactcttca tcttgtttct ttttttgctc
      541 aatttcattg agcctatttt tatacgcttt ttgtaattga ttatattcat caggacaaag
      601 ttttctcatg tgatcaaatt tttgatctcg ttttcttgat atttcattgt ctctatttag
      661 tcttgcagcc acttcacgta actgcgtgtt ctcagtttgt aacctcttgt tttcaattga
      721 taaatttact ataccatcat gcatggggtg aattttttca tttacttttt tcttatattc
      781 ttgaagattt tcaattgtac tttttttagc ttcttcagga gttattgttt taaaaattct
      841 acctgtaggt gttggggttt taatattaat tgctacttta aaatctttct tgaattcttc
      901 ttttgtttct tgccctattt cttttgcatc ttcaattgaa gaataaaaag tcttaatatc
      961 ttgatgttta gcaactgatt tttctatacc tctgcttaac ccgaattttt ccatagattt
     1021 tgcataatct gtttgaagtt cacttaataa ctttttacca cctaagaatt ctttcattga
     1081 taatctatta ttgtttttat ctgaaataat aggagtaatt attgcatgaa ggtgtggtgt
     1141 tttttcatca ttatgaataa tgacattaat taaattttca ccgtatttat ttttcaggaa
     1201 atttaaattt tcatttttcc actctttaaa actattatct gtattaaaga attcaggaga
     1261 tgcagttaaa atcatctcag cacataatac agaatccttt cttattttca ctcccgactc
     1321 ttcgagtctt ttatttacat catcacgaac gaacggagaa ccatataaaa tttcattatt
     1381 acttgttttt aatggatctg cattgaatgt ttcacgctgt cttaaattat ggctttgagc
     1441 ttgtgaaagc tgagattgtg atttaatttt ccctactcta caaattgcat acatatttac
     1501 ctacctttta ttttcttttt taatttactt atttcactat aactatttct aatttcttgg
     1561 cttttatctt tagtggaaat acagctagaa ataatccaac ccatcaacat aaaaccaaat
     1621 aacatttcca aaactataac tattaccatt ttaaattctc catgtagagc tgtgcacgaa
     1681 cggaacctta cgaagtaagg tgtagtgagt gtacctttac agattaccgt tatacttttt
     1741 aattaacaca gaataggaaa atagcaacat tgcaaattag ttaaagctga aaatataaag
     1801 tcaaaagatc aatccctcac aagcgaaaaa taaatttttc acttgttcac gattaccaat
     1861 ttaaaaccaa ttgatcctac ccctctgacg ggtctttctg gatagatacc ataaccccct
     1921 ctaaaacgct ctgtaacggt ctgtgatgag ttttagggct atgacctcat aagagcctga
     1981 aaaactaccc tctgacgggt ctgtaatgtt tagtccggta aacctgcagg ccgcttttga
     2041 tgatgtttga gacatcattt aggcggcagt atggcctatg gccaaaagag tattagccag
     2101 agttagaaga accaatcgag aaccgcgaga actacagtag cttgctactg tggttcgagg
     2161 ggttggcctg cccggcgagg gcggttttac cctttcttat gtttcttact aaaatcttac
     2221 ttcttaacca cccctctagc ccttgtccca caaggctttg agccttaaaa agttataggt
     2281 atcaataact attgttatag gtatcaataa ctcattgtta taggcatcaa taactccagt
     2341 tataggtatc aataactttt ttgatttagt catatgttca aaaatctata actttgttat
     2401 agtttttttg ttttagttat tgacaaatat ttatgatcgg ttatttaaat aatatgtctt
     2461 ttatatatta cgattaaata aggaggttat atgttaaaag aacatgcaaa aatagataac
     2521 gttttagatt tcgatttgtc tctaaataca gttgaagaaa taaacaagat gcaagaaaaa
     2581 ggtgacgtaa aagtcactac aatagtgcaa aacgaaaaaa aggtttcttt aaaggatcag
     2641 ttcgttatgg cttttacttc taacttaaga gcattagctg agttaaatat tagtcaaaat
     2701 gagcttaaag taataactta tattcttgaa gttatggagt ttggaaacct cattagcatt
     2761 aatcaaacat caatagctaa agagcttgga ttacataaat ccaatgtttc atatactttt
     2821 aagagacttg tagaaaaaaa tattctaata aaaaaagatg gtcatacatt tatgaactct
     2881 aacttatttg caaaggggtt aaatcatagt atgaacgaag aaag
//
