LOCUS       NZ_OW967971             3638 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 648 plasmid P5, complete sequence.
ACCESSION   NZ_OW967971
VERSION     NZ_OW967971.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065716
            Assembly: GCF_905330275.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330275.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:25:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,224
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,875
            CDSs (with protein)               :: 4,875
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 71 of 233
            Pseudo Genes (incomplete)         :: 168 of 233
            Pseudo Genes (internal stop)      :: 39 of 233
            Pseudo Genes (multiple problems)  :: 40 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967971.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="648"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(3486..3638,1..165))
                     /gene="sugE"
                     /locus_tag="LQ127_RS25925"
     CDS             complement(join(3486..3638,1..165))
                     /gene="sugE"
                     /locus_tag="LQ127_RS25925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_000118520.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIAM
                     LSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKLST
                     H"
                     /name="sugE"
     gene            complement(<276..707)
                     /locus_tag="LQ127_RS25930"
                     /old_locus_tag="AI2858V1_5024"
                     /pseudo=""
     CDS             complement(<276..707)
                     /locus_tag="LQ127_RS25930"
                     /old_locus_tag="AI2858V1_5024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(710..1636)
                     /locus_tag="LQ127_RS25935"
                     /old_locus_tag="AI2858V1_5025"
     CDS             complement(710..1636)
                     /locus_tag="LQ127_RS25935"
                     /old_locus_tag="AI2858V1_5025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001314449.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1812..2207
                     /locus_tag="LQ127_RS25940"
                     /old_locus_tag="AI2858V1_5026"
     CDS             1812..2207
                     /locus_tag="LQ127_RS25940"
                     /old_locus_tag="AI2858V1_5026"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094321956.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYQKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            complement(3309..>3407)
                     /locus_tag="LQ127_RS25945"
                     /pseudo=""
     CDS             complement(3309..>3407)
                     /locus_tag="LQ127_RS25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            <3406..3489
                     /locus_tag="LQ127_RS25950"
                     /pseudo=""
     CDS             <3406..3489
                     /locus_tag="LQ127_RS25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          complement(714..1636)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacaggcaac gtgcgcattg cccaagagag catggcgata ctgacgatca tcgccgcaat
       61 agtgataatg cttggcgtaa gacgcgtaaa accgtgggtg tatttcaggc caatcgccca
      121 gacaacttcg agcaaacctg caattaataa aacgatccag gacatatcag gctccagaac
      181 aatgggtccg tccccggtga aagaagcgtt tgcaggtcgt cctgcaaagc taatgtgtga
      241 aatggcattt ttgcccggaa gaaaatgaat ttcaagagca ttgttttcat tccccatcca
      301 agcacacaga acataaccag aaaagccact gacaccccac ccagccattt ccagctatac
      361 cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat atccctgttc
      421 agggcatccg cgtttgcctt catgtttgcg actgactctt ccagccgctg aaaatcctgg
      481 ctgatgcgct cacctatcag ccctgcaaca tccagcttct gcacctcgtt tctgacttca
      541 tccctgacct gttttgccac accgtcagaa atatcccgat atccgcgaag tgtttccctt
      601 gccacagcca gagaatcagc aacagctttc tgttgctcca ttgcggtatg ttgcagatgc
      661 tccaccgata acaggagcgt gagtaatttt tcttctgtgc tggccatgtt cagaatccca
      721 tagaaaatcc acgtgacggt gatttaaccc gttcaggttc agttcggttc tgtctgctca
      781 ctctgtctct catgtcaaag ccatgctccc gcgcaacatc atgttgcagc cttctgacca
      841 gttcatgttg cttaaccgga gtgtccagcc attccctgat gtcatgcccg accgttcgcg
      901 cctgttcaag atggcgggaa tagtccggtt cagaccgttt ttgcatgatg tcatgacgct
      961 gggcatcatc aagccgtccg gcagcttcaa aacccagatg cacttcggga cgtcgttcaa
     1021 tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct ttcaaagcca tgctttctca
     1081 tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc tctttttgct
     1141 gagtgagagt caggcttccg gaatcctttt tcgcacctcc ccgctcggga tacctggcgt
     1201 tatagcgccg gaaatactgt tccggtgtac gttcaatgcc atcattcaca cgctggctca
     1261 tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct cgctttcgga ttgtgaatgg
     1321 caaaactcca ggcgtgccgt tcgccagctt cctgacgtac aaaatcctgc accagttcaa
     1381 ggcgctgttc cgctgtcagt tcacggggta atgcaatttc aagttcccga taggtcgaac
     1441 cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg tgagggatta tctcgcgccc
     1501 attccggcat gtttccgtgt gcggaatatt ccaaatcctg acggtcgcgg tatttctctt
     1561 tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt gccaccaaat ttaacgctga
     1621 ggtgataggt tgccacggtt tcagccctcc gcaggagcgc actgtttctc gacgaaggag
     1681 agaaacatat aagtgcgctt ccttctaatt ttacgaatta gcacggaaga taccccctgc
     1741 acccaccgac cgcaagcgat atactgcaca accccaacac accgcaaaaa acaccaggga
     1801 gcatcccaaa catgcgcatc agcaacatcg actggttgaa gaaacgaatc ggctttatca
     1861 ggaagctcgg agaacagacc gcacgacaga ggcagatcat tgacctgctc gataacgaag
     1921 ccggactaac agaacaggag cgcaaactac tccacgtcct ggcaaccgca gaaaaaaacg
     1981 agctacaggc acaggagaac gcacgcaaac aggccaacca aaaacggatg gaggggaaaa
     2041 cacaacgacg ggaacgcaat caccgcctgt ttcttgcggc ggggttactg atcgaggccg
     2101 gactagtgga cacaaaaaca ggcgaactga gataccaaaa agacaatata ttacagaaac
     2161 ttaaggcaat caggctcgat cttgaaacaa gcccagatca tcattaattc cgctgcgtgg
     2221 tggtgcgttt gcctgccggt gttcaatgtt ttcgatttcg cgacgcgcta cgcgcggatc
     2281 tggcataggt gcgtacgtgc tcaatgtggt ttccggttca cttccgcttc ctcgcccact
     2341 cgctcactac gttcggtcgc tggggctgcg gcgagcggtg ggatcactct cacatgatgc
     2401 agatcctttt tttttttgct ctggagttat cagttgttgc ggtgctcatg ggatcgtgcg
     2461 atatgctgga tggtggaagg tcggagggaa cggcaacaac tcacccctcc cagtgaaggg
     2521 acgggtagtt tgccgctctc gatatgccgg acggagccaa ggccatcgat cacggggcgc
     2581 gtcggtggaa gaacccgcgc ctgcgtgacg tatgcgtctt cgccgcacca tctcacccgt
     2641 cacgccacca cataccggga actgccgcca aggtctaact caaccaatct agccgcgcta
     2701 catacggttt ttgtggcaac tcatcaggtg tccgctgtcg atgaaactct ctgcgccggt
     2761 agcggaactc tcacgcgtgc ggttgccgtc gtcacagctc ccccaaccgc aaacggtctt
     2821 tgcaaacacg caatcatctg caaaaacagg attttttcgg gattctgggg aggctgtgcg
     2881 gtcggtaatg cttgaagaat acagaaggtt catgcaaact gaatgaactg gtttccgttg
     2941 tcactgcaac agactccaca ggaacattga ggaggtcgaa aacctcaatg aacccgaaga
     3001 agctcgccta acggcgggct tttttcgttc tacgctccct gacgaaagat cgcaagcaca
     3061 ataacgcgat ttttcacgct tttacgctca aaacatcaaa aaaccatgct ttcccccacc
     3121 cctacctcaa aaaacgcgtc agaaacgttt ccaggggctt gcataactat tcacaaaagt
     3181 gattcggaat caatttcgcc aaatctgagc accgaacggg tcaaaacggc aaactttctc
     3241 atcagaaaac ggtgaacggg acgcggaatt ttttcgcgtc ccgtgaggcg ttggacaagc
     3301 gaagcgcgtc agtttttccg gctcttccct gttgctggtt tctccgccag tttatcttcc
     3361 agtgtcacaa ctttctgata caccgccgct gtggcgttgt agttcctgaa acagcaaatg
     3421 ttagccatcg cgacccggga agggtttgaa gtgaataaac tgatttgggt gaaacagcct
     3481 ggcgcttagt gagtgctcag cttcagacca ataatgccag caacgatcag cccaaggctc
     3541 agcaaacgtg ccgggctggc agactcaccc agcagcaaaa tccctgtaat ggccgcccca
     3601 acagcgccaa taccggtcca gaccgcataa gcggttcc
//
