LOCUS       NZ_OW967970             5593 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 648 plasmid P4, complete sequence.
ACCESSION   NZ_OW967970
VERSION     NZ_OW967970.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065716
            Assembly: GCF_905330275.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330275.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:25:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,224
            CDSs (total)                      :: 5,108
            Genes (coding)                    :: 4,875
            CDSs (with protein)               :: 4,875
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 71 of 233
            Pseudo Genes (incomplete)         :: 168 of 233
            Pseudo Genes (internal stop)      :: 39 of 233
            Pseudo Genes (multiple problems)  :: 40 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967970.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="648"
                     /isolation_source="Hospital Universitario Mutua de Terrassa
                     (Terrassa)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P4"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(199..522)
                     /locus_tag="LQ127_RS25890"
                     /old_locus_tag="AI2858V1_5017"
     CDS             complement(199..522)
                     /locus_tag="LQ127_RS25890"
                     /old_locus_tag="AI2858V1_5017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_023485641.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSEQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            586..777
                     /locus_tag="LQ127_RS25895"
                     /old_locus_tag="AI2858V1_5018"
     CDS             586..777
                     /locus_tag="LQ127_RS25895"
                     /old_locus_tag="AI2858V1_5018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1448..1606
                     /locus_tag="LQ127_RS26190"
     CDS             1448..1606
                     /locus_tag="LQ127_RS26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071593719.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(1896..2876)
                     /locus_tag="LQ127_RS25900"
                     /old_locus_tag="AI2858V1_5019"
     CDS             complement(1896..2876)
                     /locus_tag="LQ127_RS25900"
                     /old_locus_tag="AI2858V1_5019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="WP_002670781.1"
                     /translation="MARNATNKLLHKAKKSKSDEFYTQYCDIENELQYYREHFSDKVVY
                     CNCDDPRVSNFFKYFAVNFDNLGLKKLIASCYVENKEGFSSSEAAKNGFYYEYHKENGK
                     KLVFDDISVSSFCGDGDFRSSESIDLLKKSDIVVTNPPFSLFREYLDQLIKYDKKFLII
                     ANVNSITYKEVFNLIKENKIWLGVHLGRGVSGFIVPEHYELYGTEARIDSNGNRIISPN
                     NCLWLTNLDVFIRHKDLPLTRKYFGNESSYPKYDNYDAINVNKTKDIPLDYNGVMGVPI
                     TFLHKFNPEQFELIKFRKGVDEKDLSINGKCPYFRILIKNKRLQK"
                     /name="adenine-specific methyltransferase EcoRI family
                     protein"
     gene            complement(2906..3739)
                     /locus_tag="LQ127_RS25905"
                     /old_locus_tag="AI2858V1_5020"
     CDS             complement(2906..3739)
                     /locus_tag="LQ127_RS25905"
                     /old_locus_tag="AI2858V1_5020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme EcoRI"
                     /protein_id="WP_001565219.1"
                     /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKA
                     LSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEW
                     RVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHF
                     PYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKF
                     VNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK"
                     /name="type-2 restriction enzyme EcoRI"
     gene            complement(4356..4589)
                     /locus_tag="LQ127_RS25910"
                     /old_locus_tag="AI2858V1_5022"
     CDS             complement(4356..4589)
                     /locus_tag="LQ127_RS25910"
                     /old_locus_tag="AI2858V1_5022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001565220.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQQDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4596..5114)
                     /locus_tag="LQ127_RS25915"
                     /old_locus_tag="AI2858V1_5023"
     CDS             complement(4596..5114)
                     /locus_tag="LQ127_RS25915"
                     /old_locus_tag="AI2858V1_5023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001389085.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     Typing          complement(870..953)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.09e-36"
     Typing          964..1520
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.464"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(203..552)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.453"
                     /coverage="99.0"
                     /evalue="7.67e-63"
     Typing          complement(4253..5592)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.8"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgtcctcccg gcggcagctc cttctccgcg aacgacagaa cacctgacgt gtatttcttc
       61 gcaaatggcg tggcatcgat gagttcccgg acttcttccg gatcaccctg aagcaccgtt
      121 gccccttccc ggttacgctc ccttcccagc aggtaatcaa ccggaccact gccaccgcct
      181 tttcccctgg catgaaactt aactatcatc ccgcgctcca tgttccctga cgacctgccg
      241 taagctgcgc aactctctct cgatggccat cagtgcggcc accacctgaa cccggtcacc
      301 ggaagaccac tgctcgctgt tcaccttcct cgccgtctga ttcaggttat tcccgatggc
      361 ggccagctga cgcagtaacg gcggtgccag tgtcggcagc ttcccggaac gggcaaccgg
      421 ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac agcgttcaag
      481 taaccgtgca tgttcatcat cagtaacccg tatcgtgagc atcctctcgc gtttcatcgg
      541 tatcattacc ccatgaacag aaatccccct tacacggagg catcagtgac taaacaggaa
      601 aaaacagccc ttaacatggc gcgctttatc agaagccaga cattaacgct gctggaaaag
      661 ctcaacgaac tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgcc
      721 gatgagcttt accgcagctg cctcgcacgc ttcggggatg acggtgaaaa cctctgacac
      781 atacagctcc cggagacggt cacagcttgt ctgtgagcgg atgccgggag cagacaagcc
      841 cgtcagggcg cgtcaggccg ttgaccttgc gggtgtcggg gcgaagccct gacccagtca
      901 cgtagcgata gcggagtgta tactggctta accatgcggc atcagtgcgg attgtatgaa
      961 aagtgcgcca tgccgggtgt gaaatgccgc acagatgtgt aaggagaaaa tgcaacttcc
     1021 gacgctcttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
     1081 cggtatcagc tcactcaaag acggtaatgc ggttatccac agaatcaggg gataacacca
     1141 aaagaaacat gtgagcaaaa aacaagaacc cagagaaggc cacgccgctg gcgtttttcc
     1201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1261 acccgacagg acttaaagat accaggcgtt tccccctggt ggctccctcg tgcgctctcc
     1321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1381 gctttctcat agctcacgct gttggtatct cagttcggtg taggtcgttc gctccaagct
     1441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1501 tcttgagtcc aacccggtaa gacacgactt aacgccactg gcagcagcca ctggtaactg
     1561 gacaaggtgg attgagatat taagagttct tgaagtggtg gcctaactgc ggctacacta
     1621 gaaggacagt atttggtatc tgcgctccac caagccagtt accttcggaa aaagagttgg
     1681 tagctcttga tccggcaaac aaaccaccgt tggtagcggt ggtttttttg tttgcaagca
     1741 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaacc
     1801 ttcgaaaaaa ccaccaggag aggtggtttt ttcgtttctg gtaccgaagc ttacgacgaa
     1861 gctaatgatc tcaagaaaaa actaacaaac atcaattact tttgtaatcg tttgtttttt
     1921 atcaaaattc tgaaataagg gcatttacca tttatagaca aatctttttc atcaacaccc
     1981 tttctaaatt ttattaactc aaattgctca gggttaaact tatgcaagaa tgtgatagga
     2041 acccccataa ccccattgta atctaatgga atatcctttg ttttgtttac atttatagca
     2101 tcataattat catattttgg atatgaactt tcattcccaa aatattttct tgtaagaggc
     2161 aagtctttat gcctaataaa gacatctagg ttagttagcc ataagcagtt gtttggcgag
     2221 ataattctat taccattaga atcaattctc gcctcagtac catataattc ataatgctct
     2281 ggaacaataa atccagaaac acctctcccg agatgaaccc caagccaaat cttattttcc
     2341 ttaattagat taaacacctc tttatatgtt attgaattaa catttgcaat tataaggaat
     2401 ttcttatcat acttaattag ttgatcaaga tactctctaa ataacgagaa tggaggattc
     2461 gtaacaacaa tatctgattt ttttagcaga tcaatgctct ccgaactgcg aaaatcgcca
     2521 tcgccacaga aagaagaaac actaatatca tcaaaaacta atttctttcc attttcttta
     2581 tgatattcat agtaaaatcc gttcttcgcg gcttcgctac tagaaaaacc ttctttattc
     2641 tctacatagc aagatgctat taactttttc aagccaagat tatcaaaatt cactgcaaaa
     2701 tatttaaaga aattgcttac tctaggatca tcacaattgc aataaacaac cttatcagag
     2761 aagtgctctc tgtagtattg cagttcgttc tcaatatcac aatactgagt gtaaaattcg
     2821 tcgcttttcg attttttagc tttgtgcagt aacttgtttg ttgcatttct agccattaat
     2881 taaaacctta ttaaaataaa aaatatcact tagatgtaag ctgttcaaac aagtcacgcc
     2941 ccaacactct gagcgaagtc gttgatatat caaacattat ttcaaacata attttcgaat
     3001 cccactccct cccatctcct tgagtatata tagatgctgc ttgtagcata atgcttttgt
     3061 ctttatgatt tacaaatttg ttaatacata gattactatt tataggcatt ccataattag
     3121 ctgcagttag tcgatctaac ctatttaata taccagaatt atactcaaga ttaacaaccc
     3181 ttccatctgg tcttgttatt gagatatttt ctgttaaaaa gttagacccc tctaagaaaa
     3241 ggacgtaagg aaagtggctc tcagagagca taaaattcgc tatctctgat atattcttat
     3301 gagatctttc gatagcatta ccagcagcca ttaaatcttg atctcctctt ttcccaacta
     3361 acaaaccatt ccttatattt ataatatctt taccttggtg tttggcttca gcaacaagta
     3421 caactctcca ttcaccataa tcatctttga cctctacaat tccaccatca ggtttgatgc
     3481 tggaatttga aacaaataaa gtaccgccaa gatcagggtc aatttttttt aaagcttcat
     3541 ttatttctgt tttctttata ctatctctat atcgaaatga taattgaggg tattcgttgc
     3601 taagagcttt ctttactaat tttgaaacct caccaacagc gagatcatga gcttttgcat
     3661 aatccccaaa aatcccaatt acaccttgag ataacttatg ttgttcagtt agcctatttg
     3721 actgtttttt attagacatg aatccatgtt tccactgtta ttttataaag ttacatatgt
     3781 cgatagatta acatacgttt gatttaccat aatcccttat tgtacgcacc gctgaaacgc
     3841 gttcggcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc gaaaaactgc
     3901 cgcgatcgca cccggtaaat tttaaccaca tgcatagcta tgcagccatg tgaatcacgc
     3961 aggaattgcc agccagagac agctgaaacg gatttttttc atgctttcgg aagcgaagaa
     4021 gacgggaccg gagcgggaaa acagaagttc aggggaatga gcgcgatctg gcaatagagg
     4081 caaaacacag caacaaaaga cacaccagaa tcgcgcccgt atgcgtttta acgcgttcct
     4141 gtgttttcag caaggtctga ctgaagcgcg ttcccggtgc ccccgaacca accattatcc
     4201 caaaaaatgc accggaaacg cgctgacaac attttagcct gtctctgatt accatcccag
     4261 cgaagggcca tccagtgtcc gttcctgtat ttccagctgg cgctcacgct ccagggataa
     4321 ctcatgttcg cgctcctgta tcagccgttc gtcccttatc tcctgttctc gctgtataac
     4381 tggctcaagc gttctgtccg ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc
     4441 tgctcgttca gcatcgcgtt gtcctgttgc gtaagcgaaa acattttctg caattccacg
     4501 aaggcgctct cccattcgtt cagccgctgc atatagtcct gttgcagttg ctctaaggcg
     4561 ttcagcaaat gtctttccag ctccgtcact ctgtgtcact ccgtcagttg tacccagtcc
     4621 ttcccctgat agggaatcac cgttgccgat ttcccctgcg ggatgaccag aaatttcctt
     4681 ctcccgtcct gcacaaactc cacgccccat gtcttcgcgt tcagtttctg caatgtttct
     4741 tcctgtatcc tgatttcttc caggttcgcc tgtatcatcc cgccaagata ccagagcgtc
     4801 ccgccactca cggtaaacag gaaaatgacc atccccagta acatcatgcc cgtattccct
     4861 gccagcttta acacgtcctt cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt
     4921 tttttcagtg attcctctgt cgaagctgtg aacagggcta tagcgtcttt gattttcgtc
     4981 tcgtttgatg tcacagcctt gcttacagat ttttcgagct tgctgaactc gtcgttcagc
     5041 attgtctctg tagattcggc tctctctttc agctttttct cgaactccgc gcccgtctgt
     5101 aaaagattgc tcataaaatg ctcctttcag cctgatattc ttcccaccgt tcgggtctgc
     5161 aatgctgata ctgcttcgcg tcaccctgac cacttcaagc cctgcctctg tgagcgcctg
     5221 aatcacatcc tgacggcctt ttatctcccc gacatgataa agagcgtcta tcccgcgtgt
     5281 gacgctctct gcaagcgcct gtttcgtttc cggcaggtta tcaggaaggg tcagcgtccg
     5341 gcggttctcc ggggcgttgg ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca
     5401 ggcatcaatt ctcggcctgt ctgcccggtc gtagtacggc tggaggcgtt ttccggtctg
     5461 tagctccatg ttcggaatga caaaattcag ctcaagccgt cccttgtcct ggtgctctac
     5521 ccacaggatg ctgtactgat tcttttcgag accgggcatc agtacacgct caaagctcgc
     5581 catcaccttt tca
//
