LOCUS       NZ_OW967798             8547 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 538 plasmid P3, complete sequence.
ACCESSION   NZ_OW967798
VERSION     NZ_OW967798.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065748
            Assembly: GCF_905330545.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330545.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:27:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,829
            CDSs (total)                      :: 4,717
            Genes (coding)                    :: 4,557
            CDSs (with protein)               :: 4,557
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 68 of 160
            Pseudo Genes (incomplete)         :: 108 of 160
            Pseudo Genes (internal stop)      :: 30 of 160
            Pseudo Genes (multiple problems)  :: 38 of 160
            Pseudo Genes (short protein)      :: 2 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967798.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="538"
                     /isolation_source="Hospital Universitario Ramon y Cajal
                     (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P3"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            552..1082
                     /locus_tag="LQ148_RS24030"
                     /old_locus_tag="AI2890V1_4584"
     CDS             552..1082
                     /locus_tag="LQ148_RS24030"
                     /old_locus_tag="AI2890V1_4584"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058813529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTISTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            1089..1322
                     /locus_tag="LQ148_RS24035"
                     /old_locus_tag="AI2890V1_4585"
     CDS             1089..1322
                     /locus_tag="LQ148_RS24035"
                     /old_locus_tag="AI2890V1_4585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021534848.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2950..3378)
                     /locus_tag="LQ148_RS24040"
                     /old_locus_tag="AI2890V1_4586"
     CDS             complement(2950..3378)
                     /locus_tag="LQ148_RS24040"
                     /old_locus_tag="AI2890V1_4586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_021536732.1"
                     /translation="MKRSGGTRYLYLISLITVAAALTACTPKGSVEQYTRHYVYASDDR
                     SDPNFYTNKADTTRMMIPFFQQFREMGEKDKAAGVSAETAQQRIKEFHSEKFLQSLRST
                     TTFAGRKYTNSDMPSPEKMKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3459..3896)
                     /locus_tag="LQ148_RS24045"
                     /old_locus_tag="AI2890V1_4587"
     CDS             complement(3459..3896)
                     /locus_tag="LQ148_RS24045"
                     /old_locus_tag="AI2890V1_4587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021536733.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQESEREQLQNEIAQLRQALEQEKKRGFWSRLFGH"
                     /name="hypothetical protein"
     gene            4122..5972
                     /gene="cya"
                     /locus_tag="LQ148_RS24050"
                     /old_locus_tag="AI2890V1_4588"
     CDS             4122..5972
                     /gene="cya"
                     /locus_tag="LQ148_RS24050"
                     /old_locus_tag="AI2890V1_4588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin Y"
                     /protein_id="WP_225387284.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGITSTQAKQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLLPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKDMGRVTERVVDAVNLALILNQIS
                     SGMSASQKDVDAANRIINETVKAINDVNLKIAEKKKQQVPLLSLMKQKQKEVEELKKVF
                     KNHSYHRIRDAQRAYDDARNKNDLLVSDINALQAQVSGLTARKQQAEKNKAAAEKAKAD
                     AKAKAEAEKARKEAEAKENNEKAVLTKASEIIISVGDKVGEYLGDKYKALSREIAGNIK
                     NFQGKTIRSYDEAMASVNKLMANPDLKINAADRDVIVNAWKAFDAEDMGNKFAALGKTF
                     KAADYVMKANNVREKSIEGYQTGNWGPLMREVESWVVSGIASAVALAIFSATLGAYLLA
                     VGASAAVVGIIGIIIASFIGALIDDKFIDRLNNEIIRPAY"
                     /name="cya"
     gene            complement(6000..6524)
                     /gene="cui"
                     /locus_tag="LQ148_RS24055"
                     /old_locus_tag="AI2890V1_4589"
     CDS             complement(6000..6524)
                     /gene="cui"
                     /locus_tag="LQ148_RS24055"
                     /old_locus_tag="AI2890V1_4589"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="WP_021534853.1"
                     /translation="MRKEHTDNKDLNRTLFLITFAGITPLIIIFITYTSNPKFYLISII
                     FDNTQNIPSIISAYNPVMTKVMDIYGKSAPLLALIAFTLQLRDRKLETIANREKLITAS
                     IFSPFFYAFYAYFFLWNNFELTTAGRTVRWMSDNDFTLFIFYACLYFCSFFMTYALCYI
                     PVVSYKLWKER"
                     /name="cui"
     gene            6644..6784
                     /gene="crl"
                     /locus_tag="LQ148_RS24060"
                     /old_locus_tag="AI2890V1_4590"
     CDS             6644..6784
                     /gene="crl"
                     /locus_tag="LQ148_RS24060"
                     /old_locus_tag="AI2890V1_4590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WP_010892547.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSKLTGIAV
                     Q"
                     /name="crl"
     gene            complement(6981..7139)
                     /locus_tag="LQ148_RS24205"
     CDS             complement(6981..7139)
                     /locus_tag="LQ148_RS24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            7472..7690
                     /locus_tag="LQ148_RS24210"
     CDS             7472..7690
                     /locus_tag="LQ148_RS24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072021258.1"
                     /translation="MFFPALSPDSVDNRITAFECAGTARRSRTTERSESVSEEAEECRK
                     LHFLLTHLCGISHRLWCTFHTIRTDAA"
                     /name="hypothetical protein"
     gene            complement(7842..8033)
                     /locus_tag="LQ148_RS24065"
                     /old_locus_tag="AI2890V1_4591"
     CDS             complement(7842..8033)
                     /locus_tag="LQ148_RS24065"
                     /old_locus_tag="AI2890V1_4591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_042634456.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8065..8421
                     /locus_tag="LQ148_RS24070"
                     /old_locus_tag="AI2890V1_4592"
     CDS             8065..8421
                     /locus_tag="LQ148_RS24070"
                     /old_locus_tag="AI2890V1_4592"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_232350939.1"
                     /translation="MGVMIPMKRERMLTIRVTDDEHARLLERCEGKQMAVWMRRVCLGE
                     PVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRR
                     LRLAVREQGTRDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          7670..7757
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          complement(7031..7413)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.354"
                     /coverage="89.0"
                     /evalue="1.03e-178"
     Typing          complement(7454..7644)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.188"
                     /coverage="99.0"
                     /evalue="3.36e-70"
     Typing          3..1405
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.421"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8066..8418
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.458"
                     /coverage="100.0"
                     /evalue="1.0799999999999999e-69"
ORIGIN
        1 cacgccattt gcgaagaaat acacgtcagg tgttctgtcg ttcgcggaga aggagctgcc
       61 gccgggagga cgtgaaaaag tgatggcgag ctttgagcgt gtactgatgc ccggtctcga
      121 aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac aagggacggc ttgagctgaa
      181 ttttgtcatt ccgaacatgg agctacagag cggaaaacgc ctccagccgt actacgaccg
      241 ggcagacagg ccgagaattg atgcctggca gacgctggtt aatcaccatt acgggctgca
      301 tgaccccaac gccccggaga accgccggat actgactctc cctgataacc tgccggaaaa
      361 gaaacaggcg cttgcagaga gcgtcacacg cgggatagac gctctttatc atgtcgggga
      421 gataaaaggc cgtcaggatg tgattcaggc gctcacggag gcagggattg aagtggtcag
      481 ggtgacgcga agcagtatca gcattgcaga cccgaacgga gggaagaata tcagactgaa
      541 aggagcgttt tatgagcaat cttttacaga cgggcgcgga gttcgagaaa aagctgaaag
      601 agagagccga atctacagag aaaatgctga acgacgagtt caggaagctc gaagaatctg
      661 taaacaggga actgacctca aacgaatctt taatcaggaa cgctatcaac gaccatacaa
      721 cagccctgaa agaactgctg gagagatatc agaaaacgac catcagcacg atggatacac
      781 actggcaaac cgtactgaaa atgagcgtga aacgctggct gtggctgatt atcgtctcag
      841 tgctgatgtt tgccacgacc gggagtctgc tgtggtatca gggcatgaag ataaatgcaa
      901 acatgaatat tctccgcgag cagaaagaga gtctggagaa gctgaacgcg aagacatggg
      961 gcgtgcgtta tcacgaggac agcaacgggc gttttcttgt gttgccgaag gggatgaaag
     1021 ccgaaacgaa ctggacggag gacaacggca aactgaatgc agtgagactg gtacaggagt
     1081 gacagagcat gacggagctg gaaaaacatt tgctgaacgc attagagcaa ctgcaacagg
     1141 actatatgca gcggctgaac gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt
     1201 cgcttacgca acgggacaac gcgatgctga acgagcgggt catgaagttg agtcagcagg
     1261 tgcagcactt gagcgagcgg acagagcgct tgagccagtt atacagcgag aacaggagat
     1321 aagggacgaa cggctgatgc aggaacgcga acatgagtta tctctggaac gggagcggaa
     1381 gctggatggc ccttcgctgg gatggtgact gaaagtccgg ttgagtcgta aacagaatat
     1441 tttccagaaa tgtcaggctg cctattggta atataaccag tcgttcttgc ggtttattga
     1501 tttttgtaca ggttgttgct gttttttttg taaagaggac aggtattttt ctgaaaatga
     1561 acttgcggga atgcgcgggt tatgtaaaaa atataattgc gtatctccga tatgtttgta
     1621 ataccgatgt ggacacagcg aatctggaag gggtcggaga tgcgcgttct ctcatgtgtt
     1681 aatattgagt tcttacatgt ggttgcatat tttttttgca tatttgaagt ctgcgaatta
     1741 tattctgata tgcaaaagtc aggactagac atactatcca tcgattcagt ccttacctta
     1801 cgccctcact gatttcctgc cgtgagggct tttttcatgg gtgtgaaaaa aggaagcccc
     1861 acctgacgga ggggctgagt gatacagagc aaaaaaatat cctggtgttg gtcacctctg
     1921 gagagtaata agattaacca taaaacaaac tataaacaag aatattgatg ccaggaattt
     1981 tcctggttat ttttttgttt tttacaaaaa agacaaaata acagaaagta aaatctgatc
     2041 atttttgttg attgttattg ctctgtaccg ttattcttaa gctgaaagtg aattcttcta
     2101 attttttctt atattcagac gcaattaaca gtccctgctc cttgagggac ttttttttgt
     2161 ctttttctct ctgttttata ttgttttgag tagtaactat aaaggcagtg aagttctgtc
     2221 ttatgagcgc gtttctggcg gtgatgcgac taacccattc tacatcaacg aacaattcac
     2281 cagaaacgcg cctgtaaatt tttagctgag caagggctct gtgtcgagag ttgtggatgt
     2341 ctggttcgcc attttaaaaa acttcttccg gcggttttta gtttcgatgc aggtcagggg
     2401 gcgctgtaaa gttatgttcc ctgattactg tgccagaatc ctgagacaga caggaggtct
     2461 accggttatt tttcgaattg ggcgcttccg gaagagagag tttcattggg cgttggtcgc
     2521 ggatggtgag cacgtttcga tgttgtgtga cgaacattca gctcagttga tggagactgt
     2581 cagaaacgcg ctcagaagtg ttgtaacgcg atttctggta tcaggcaatt gtttcctttt
     2641 tctggagcat gatacctgtg tgtacggtga tgagttctgt cacgtacagt aatatttccc
     2701 tcctgatgct tccatctggc aggagttgaa cctgccgaaa atatgaaaaa acccccgttt
     2761 cagttacatc aggcaggcaa ttcgttcgtg attcgcatgg ctgcatagat atgcatgagg
     2821 ttaaaattta tcaggtgcga tcgcggcggt ttttcgggtg gtttgttgtc atttttacct
     2881 gtctgctgcc gtgatcgcgc tgaacgcatt tcagtggtgc gtacaattaa gggattatgg
     2941 taaatccatt tactgtcttc cctcgtagcc atcgagataa accgcagaaa tcgtgtcagc
     3001 cagtagcttc attttttccg gtgatggcat atcactgttc gtatattttc tgcccgcaaa
     3061 ggtggttgtg cttctgagag actgaagaaa tttttctgag tggaattctt ttatgcgttg
     3121 ctgtgccgtt tctgctgata ctccggctgc tttgtctttc tcccccatct ccctgaactg
     3181 ctgaaagaac ggaatcatca tccgtgtggt gtcggccttg ttggtgtaga agttagggtc
     3241 agaacggtca tcggaagcgt acacataatg tcgggtatat tgttccacac tgcctttcgg
     3301 ggtacaggcc gtgagggctg cagccacagt tatcagggat ataagataca gatagcgtgt
     3361 gccaccagaa cgtttcatac gtgtctcctt accttaaagt atttattctc ggtgagttat
     3421 tatccgaata ctatcttatc atcagcctct gaccgagttc agtgaccgaa gagtctggac
     3481 cagaatcccc gtttcttttc ctgttccagt gcctgcctga gctgggctat ctcattctgt
     3541 aactgttccc gttctgattc ctggtgcctg cgatccatat cctgtgcctg tttatcctca
     3601 agcatcagcg tcaggcattg tttcagctca ttcagttccc ggagaatgcg ttctgtctgc
     3661 tgatcatgtg gattttctgc ctgtcccaca gtgtgccttt cttgtgtctc attttgcttt
     3721 aattcgccgt atgcccggat cagttcactg gtttcaaact cccttcgtcc gtcctggctg
     3781 gtacggtaac tgacaagacc tgagcgcata tcacggtaaa actgtgaacg cgatcttcca
     3841 gtcattttca gtgcgtcccg gagtgtgtgc catgccataa agttacagtg tcccataggt
     3901 gtctcatctc atggtttcag taaaacataa tgaggtctga gaacggtaat gtttgcgccg
     3961 gtttttgtgg tatccggcgg gaatagcgcg tggtgtgaga gactgttttt ttgatcgttt
     4021 tcacaaaaat agaagtccac agtcttgaca gggaaaatgc agcggcgtag cttttgtact
     4081 gtacataaaa ccagtggtta tgtatacagt atttgttttt aatggtttgt tttaaaagtc
     4141 aaagaggaat gaatatgccg gggtttaatt acggtgggta tggtgacggt actggctgga
     4201 gttctgaaag tggtggtcca gctccaggcg gtggtatgca tggaaatagt ggaggccagc
     4261 gcggagataa cgcaaacagt agcaattctg tgagtcagca gatttctgcc attcagaatg
     4321 atcagaaact taaacagaaa gtggtgaata tgctgattgc tgcgagaaaa atgaatcctg
     4381 aagcaaaaat gattctggga agtattgctc cttctggtgt tatgcaggtc accattgagg
     4441 ggataacctc cactcaggcc aagcagcttg gtttgggagg gctggtcatg ggatataacg
     4501 cttctggtgt tattggggct gtgggcgaaa tagacaccgg tcaccgcctt aatgcatcag
     4561 gagcaagtac tcccggaagc gaaacttctg tggagagttt tgtgaacgga cagaaacccg
     4621 ccggagaatg gcatgctgtg gcgaaggaca gctggacagg ggctggtcca gtgaatgtag
     4681 ggctggttaa taacgccatc aaaagtgtcc ggattatcaa aaaaggctat gtgactggtg
     4741 ttctcttgcc tgaagaggtg atgaataaag cagagtacaa agcaatgcgg caggctttcg
     4801 attcccttcc actggcaaaa cagggagagg ctgtcagaca gattgttgca gcctggagtc
     4861 tggcatatca ggatttcccg gtaaatctga aaaaagatat ggggcgagtt actgaacggg
     4921 tggtggatgc ggttaatttg gcgctcattc tcaaccagat atcatccgga atgtcggcct
     4981 cacagaagga tgttgatgcg gcgaaccgga ttattaatga gaccgtgaaa gccattaacg
     5041 acgtaaatct taaaatagcg gagaagaaga aacaacaggt accattatta agccttatga
     5101 aacaaaaaca gaaagaggtt gaagaactaa aaaaagtatt taagaaccat tcttatcacc
     5161 gtattcgtga tgctcagaga gcatatgatg atgcccggaa caaaaatgat ttactggtca
     5221 gtgatattaa tgcgctgcag gcacaggttt ccggactgac tgcaaggaaa cagcaggcag
     5281 aaaagaacaa ggcggcagca gaaaaagcaa aagctgatgc aaaggcgaaa gctgaagctg
     5341 aaaaagccag aaaggaagct gaagcaaaag aaaataacga gaaagctgtt ctgacaaaag
     5401 ccagtgaaat tatcattagt gtgggtgata aggtcggaga atatcttggc gataaatata
     5461 aagctctttc tcgtgagata gcaggtaata tcaaaaactt tcaaggtaag acgatccgta
     5521 gttatgatga ggcgatggct tctgtcaata aactgatggc taatcctgat cttaaaatta
     5581 atgctgcaga cagggatgtc attgtgaatg cctggaaagc atttgatgca gaggatatgg
     5641 ggaataagtt tgccgcgctg ggtaagacat ttaaagccgc agattatgtg atgaaggcaa
     5701 ataacgtccg ggagaagagt atagagggat atcagaccgg aaactggggg ccgttaatgc
     5761 gggaggtcga gtcctgggtt gtcagtggta ttgcttctgc tgtggctctt gctatttttt
     5821 ctgccaccct tggagcttac ctgcttgctg tgggggcatc agctgcagtc gtagggatta
     5881 tcggtattat tatagccagt tttattggag cattgattga tgacaaattt atagacaggt
     5941 taaataacga aattattcgc cctgcgtatt aacctagagt cgaaaggtag tcaagaagac
     6001 tacctttctt tccagagttt atatgacaca accggaatat aacacagagc ataagtcata
     6061 aagaaagagc aaaaatagag gcaggcgtaa aaaataaaca aagtaaaatc attatcggac
     6121 atccatctga cagtcctacc tgcagtggtt aattcgaaat tattccacag aaagaaatat
     6181 gcataaaaag cataaaaaaa tggactaaat atgctggcag taattagttt ttctctgttg
     6241 gctattgtct ctagctttct gtcgcggagt tggagagtaa aagcaataag tgccagtaag
     6301 ggagcgcttt tcccgtagat atccatcacc tttgtcatca caggattgta tgcggaaatt
     6361 attgaaggta tattttgtgt gttatcaaaa atgatgctaa ttaaatagaa cttggggttt
     6421 gatgtataag ttataaaaat aatgattaat ggtgttatgc ctgcaaaagt aatcaaaaaa
     6481 agcgtcctgt ttaaatcttt gttgtcagtg tgctcttttc tcattggtat aactctccaa
     6541 gcttttcatt attattaatt tagttgtaga tgtgtataag catatcatgc tgtacaagta
     6601 atggtcatcc taagattagg ggtatgtgtt aatgagggaa tatatgaaaa tgatattatc
     6661 aggagggatg cttgtcttga tggctttgct atcaggctgc caagctaatt acatccgcga
     6721 tgttcaggga gggactgtcg caccatcctc ctcttctaaa ctgacgggga tcgcggttca
     6781 gtagaaaagt taaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcctg
     6841 taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg ttaggtcagt tggggaactg
     6901 cttaaccaag gtaactggct tcagcagagc gcagatacca aatactgtcc tttcagtgta
     6961 gccgtagtcg ggccactact tcaagaactc tgtagcaccg tttgtgccat catcgctctg
     7021 ctaatccggt taccagtggc tgctgccagt ggcgttaagg cgtgccttac cgggttggac
     7081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7141 cagcccagct tggagcgaac gacctacacc aagccgagat accaacagcg tgagctatga
     7201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7381 agcctatgga aaaacgccag cggcgcggcc tttcgttggc ctttcgttgg cctttcgttg
     7441 gcctttcgtt ggcctttgcc tttttgctca catgttcttt ccggccttat cccctgattc
     7501 tgtggataac cgtattaccg cctttgagtg cgccggtacc gctcgccgca gccgaacgac
     7561 cgagcgtagc gagtcagtga gcgaggaagc ggaagagtgt cggaagttgc attttctcct
     7621 tacgcatctg tgcggcattt cacatcggct atggtgcact tttcatacaa tccgcactga
     7681 tgccgcatag ttaagccagt atacactccg ctatcgctat gtgactgggt cagggctgcg
     7741 ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtctgct ctcggcatcc
     7801 attcacagac cagttgtgta cgtctccggg agctgcatgt gtcagtggtt ttcaccgtca
     7861 tccccgaagc gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg aagcgattca
     7921 cagatatctg cctgttcatc cgcgtccagt tcgttgagct tctccagcag cgttaatgtc
     7981 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgttttgt cactgatgcc
     8041 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg cgagaggatg
     8101 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg tgagggtaaa
     8161 caaatggcgg tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg ttccgggaag
     8221 ctgccgacac tggcaccgcc gttactgcgt cagctggccg ccatcgggaa taacctgaat
     8281 cagacagcga ggaaggtgaa cagcgggcag tggtcttccg gtgaccgggt tcaggtggtg
     8341 gccgcactga tggccatcgg ggatgagctg cgccggctgc gtctggccgt cagggaacag
     8401 gggacgcggg atgatagtta agtttcatgc caggggaaaa agtggtggca gtggtccggt
     8461 tgattacctg ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc agggtaatcc
     8521 ggaagaagtc cgggaactca tcgatgc
//
