LOCUS       NZ_OW967521            10058 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Klebsiella oxytoca isolate 2 plasmid P3, complete sequence.
ACCESSION   NZ_OW967521
VERSION     NZ_OW967521.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065822
            Assembly: GCF_905329555.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905329555.2-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,061
            CDSs (total)                      :: 5,941
            Genes (coding)                    :: 5,837
            CDSs (with protein)               :: 5,837
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 42 of 104
            Pseudo Genes (incomplete)         :: 65 of 104
            Pseudo Genes (internal stop)      :: 18 of 104
            Pseudo Genes (multiple problems)  :: 20 of 104
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967521.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /isolate="2"
                     /isolation_source="Hospital Universitario Marques de
                     Valdecilla (Santander)"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="P3"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            1..864
                     /locus_tag="LQ214_RS30200"
                     /old_locus_tag="AI2744V1_5904"
     CDS             1..864
                     /locus_tag="LQ214_RS30200"
                     /old_locus_tag="AI2744V1_5904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_013250884.1"
                     /translation="MAINIMKAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIADN
                     LTLESIMGKRLNVMDEAHLRRVIEYSAGARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELLEVPNGKKG
                     GSREQA"
                     /name="helicase RepA family protein"
     gene            851..1702
                     /gene="repC"
                     /locus_tag="LQ214_RS30205"
                     /old_locus_tag="AI2744V1_5905"
     CDS             851..1702
                     /gene="repC"
                     /locus_tag="LQ214_RS30205"
                     /old_locus_tag="AI2744V1_5905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_111987085.1"
                     /translation="MSKHDLTHARHDPAHCLAPGLFRSLKRGERKRGKLDVVHDYGDGK
                     RIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAFQQDA
                     MVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYASDEQ
                     DGKLYVALNPLIARAVMGGGQHVRISMDEVRALQTDPARLMHQRLCGWIDPGKAGRVEL
                     DTLCGYVWPEEANPEAMKKRRQTARKALAELAAIGWTVSEYAKGKWEVKRGTVEKTDKI
                     VR"
                     /name="repC"
     gene            complement(1788..2192)
                     /gene="tnpA"
                     /locus_tag="LQ214_RS30210"
                     /old_locus_tag="AI2744V1_5906"
     CDS             complement(1788..2192)
                     /gene="tnpA"
                     /locus_tag="LQ214_RS30210"
                     /old_locus_tag="AI2744V1_5906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021141233.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISAs26 family
                     transposase"
                     /protein_id="WP_102948427.1"
                     /translation="MQFTKINKSRHAAYVLHVHLVFITKYRKPILGDLHHASFGQCAAE
                     VCRGFGAELKECDGEADHVHMLIEYAPTVQLSVLVNSLKAVTSRRLRNEFLDLRGAYSK
                     PVLWSRSYFAGSCGGAPLEVIKQYIQNQRG"
                     /name="tnpA"
     gene            2251..3477
                     /locus_tag="LQ214_RS30215"
                     /old_locus_tag="AI2744V1_5907"
     CDS             2251..3477
                     /locus_tag="LQ214_RS30215"
                     /old_locus_tag="AI2744V1_5907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672025.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="WP_021141234.1"
                     /translation="MQILKAYKFRLEPTEQQAQRLRQLGGCARYVWNHGLAETKRILDA
                     GEKLPSAFELNRMLTSWKKDPEMAFLAEAYTDNLQQKLKDLHGAWMRCFDKKLAAKAPV
                     FKKKSDNRDAIRFVNFDKYCRLEKGRVKLPAGLAWVKFRQSQPVEGKIKNATVSQAAGH
                     WYISFQVEQETLEPIHPSGSMVGLDAGIAKLATLSDGTVFEPVNSFKTNQTKLARLQRQ
                     LSKKVKFSSNWKKQKAKIQRLHSHIANTRRDYLHKVTTTISKNHAMIVIEDLKVASMSK
                     SAAGTTSQPGRNVRAKSGLNRSILDQGWFELRRQLEYKQAWRGGQVLAINPAYTSQQCA
                     CCGHTAKDNRQTQSQFKCAACGYEANADVNGARNILAAGHAVLACGGVVQSGRPMKQEP
                     GTARAAITQ"
                     /name="RNA-guided endonuclease TnpB family protein"
     gene            complement(3812..4675)
                     /locus_tag="LQ214_RS30220"
                     /old_locus_tag="AI2744V1_5908"
     CDS             complement(3812..4675)
                     /locus_tag="LQ214_RS30220"
                     /old_locus_tag="AI2744V1_5908"
                     /EC_number="3.5.2.6"
                     /function="hydrolyzes ceftazidime"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor-resistant carbapenem-hydrolyzing class
                     A beta-lactamase GES-6"
                     /protein_id="WP_032490973.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVKWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MSDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="inhibitor-resistant carbapenem-hydrolyzing class A
                     beta-lactamase GES-6"
     gene            4778..5728
                     /locus_tag="LQ214_RS30225"
                     /old_locus_tag="AI2744V1_5909"
     CDS             4778..5728
                     /locus_tag="LQ214_RS30225"
                     /old_locus_tag="AI2744V1_5909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436976.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="WP_230182964.1"
                     /translation="MHYSLQTEKAYVYWAKAFVLWTARSHGGFRHPREMGQAEVEGFLT
                     MLATEKQVAPATHRQALNALLFLYRQVLGMELPWMQQIGRPPERKRIPVVLTVQEVQTL
                     LSHMAGTEALLAALLYGSGLRLREALGLRVKDVDFDRHAIIVRSGKGDKDRVVMLPRAL
                     VPRLRAQLIQVRAVWGQDRATGRGGVYLPHALERKYPRAGESWAWFWVFPSAKLSVDPQ
                     TGVERRHHLFEERLNRQLKKAVVQAGIAKHVSVHTLRHSFATHLLQAGTDIRTMQELLG
                     HSDVSTTMIYTHVLKVAAGGTSSPLDALALHLSPG"
                     /name="integron integrase"
     gene            complement(6209..6676)
                     /locus_tag="LQ214_RS30230"
                     /old_locus_tag="AI2744V1_5910"
     CDS             complement(6209..6676)
                     /locus_tag="LQ214_RS30230"
                     /old_locus_tag="AI2744V1_5910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_230182963.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYELSDGLV
                     ENLTDEERIELQSYIETERQAEEKRIRQYSAKSAASRIFEVADTIKAGDFEPSEAWAAD
                     TWAAIEALTKAMRKAGYPKPRKAPQKAADAPMPGQAGLPFGDAPETPESAS"
                     /name="hypothetical protein"
     gene            7132..7563
                     /locus_tag="LQ214_RS30235"
                     /old_locus_tag="AI2744V1_5911"
     CDS             7132..7563
                     /locus_tag="LQ214_RS30235"
                     /old_locus_tag="AI2744V1_5911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_013250886.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNEAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPAANREQWAELARTAANLNQIARHLNEGQRGDGERIGKA
                     LGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDESEG"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(7754..7987)
                     /locus_tag="LQ214_RS30240"
                     /old_locus_tag="AI2744V1_5912"
     CDS             complement(7754..7987)
                     /locus_tag="LQ214_RS30240"
                     /old_locus_tag="AI2744V1_5912"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228283734.1"
                     /translation="MLKLLGRLDGAAGFPLAQPNFPVQPYPACHDVDVILVRVLVAHCH
                     PRRIGLVKAHALHEVSRHGFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            8319..9002
                     /locus_tag="LQ214_RS30245"
                     /old_locus_tag="AI2744V1_5913"
     CDS             8319..9002
                     /locus_tag="LQ214_RS30245"
                     /old_locus_tag="AI2744V1_5913"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064483930.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSLSLMHEDLKRLPVAVS
                     TETAQTLEPLARLQQDVTQVLEAYDKVTAIQRKTLDELTEQMSVSAAKAFERKAGELDK
                     TISDLSNSLSGLETSISSMKQTAEQVVALPGNLVSAQQNMTEAANKLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            9038..9988
                     /locus_tag="LQ214_RS30250"
                     /old_locus_tag="AI2744V1_5914"
     CDS             9038..9988
                     /locus_tag="LQ214_RS30250"
                     /old_locus_tag="AI2744V1_5914"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064483931.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGIS
                     PDTIEHAEKAGMVRYADGGVLFVGYDHTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRA
                     ERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEQGKDLADINARQV
                     AEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR"
                     /name="hypothetical protein"
     ARG             complement(3816..4675)
                     /name="blaGES-6"
                     /gene="blaGES-6"
                     /gene_name="inhibitor-resistant carbapenem-hydrolyzing
                     class A beta-lactamase GES-6"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..864
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.769"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7548..9985
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctatca acattatgaa ggcgttccag caggaaccgc cacaactgga tttcatctgg
       61 ccgggctttc tggctggcac cgtgggcgca ctggtggccc ccggcgcgac cggcaagagc
      121 ttctgggcgc tggaagctgc tatgtccatt gcttgcagcg tggcaggcgg tgacgtggtg
      181 aacctcaagc cgcagcacac cgggcgcgtg gtctatctgg ccggggaaga tcccgaaccc
      241 gccctgatcc ggcgcgttca cgccatcggc cagcacctca accagtcggc ccgcgaagcc
      301 atcgccgaca acctgaccct tgagtcgatc atgggcaagc gcctgaacgt catggacgag
      361 gcgcacttgc gccgcgtcat cgagtacagc gccggggcgc ggctgatcgt gctggacacc
      421 ctgagccgga ttcacagcct cgacgagaac agcaacggcg acatggcgcg gcttgtcgcc
      481 acgctggaac acgtcgcggc cagcactggc gcgtctgtcc tgtacctgca ccacgtcagc
      541 aagggcagcg cccgcgaggg gcaaaccgac cagcagcagg cagcgcgggg cgcatcggca
      601 ctgatcgaca acgcccgctg gtgcggcttc gtcgccaaga tgaccgagga cgaggccaag
      661 cgcctgagcg accgcgccta tgaccgtcag cccatcggca acgaccggcg cgggttcttc
      721 gtgcgcttcg gcgtgagcaa acagaactac gacgccaccc cgcttgacca gtggtatgag
      781 cgccgagacg gcggcgtgct ggttccggtg gaactgctgg aagtgccgaa cggcaagaag
      841 gggggcagcc gtgagcaagc atgacctgac ccacgcccgg cacgacccgg cgcattgtct
      901 ggcccccggc ctattccgca gcctcaagcg tggcgagcgc aagcgcggca agctggacgt
      961 ggtgcatgac tacggcgacg gtaagcgcat cgagttcagc ggcccggagc cgctgggcgc
     1021 tgatgacttg cgcatcctgc aaggacttgt ggcaatggct gggccgggtg gccttgtgct
     1081 gtcacctgaa ccgcagaccg aggccgggca gcaactccgg ctgttccttg agccgaaatg
     1141 ggaagccttc cagcaggatg cgatggtggt caagggcagc tatcgagcac ttgcccgcga
     1201 aattggctac gccaaccccg acgacactaa gccggttcgg gagtgcatcg agcggctttg
     1261 gaaggtgtcc atcatcgcgc aaaacggcag gaagcggcag ggcttccgcc tgctgtccga
     1321 gtacgccagc gacgagcagg acggcaagct gtatgtggcc ttgaatccct tgattgcccg
     1381 cgccgtcatg ggtggcggtc agcatgtccg catcagcatg gacgaggtgc gggcgctaca
     1441 gaccgacccg gcgcggctga tgcatcagcg cctatgcggc tggatcgacc ccggcaaggc
     1501 cgggcgggtg gaactggaca cgctttgcgg ctacgtctgg ccggaggaag ccaacccgga
     1561 agccatgaag aagcggcgcc agacggcccg caaggcactg gccgagcttg ccgccatcgg
     1621 ctggacggtg agcgagtacg ccaagggtaa atgggaggtc aaacggggga cggtagagaa
     1681 aacggacaaa atcgtacgct aagcatgcgg cctgtcaggt tgaggccatc ttaaatgcga
     1741 aaggggactc cctccgacgt aggtgggagg ggaatttcgc cggggcgtca gccccgctgg
     1801 ttttgaatgt attgcttgat cacctccaac ggtgctccgc cgcaagagcc cgcaaagtaa
     1861 gagcgtgacc agagtacggg tttgctgtaa gcacctctca agtccagaaa ttcattacgc
     1921 agtcgccttg atgtgaccgc ctttaacgag ttcaccagta ccgatagctg caccgttggc
     1981 gcgtactcta tcagcatgtg aacatgatca gcctctccgt cacactcttt cagttccgca
     2041 ccaaaacctc tgcaaacctc tgccgcacac tgaccgaagg atgcgtgatg cagatccccc
     2101 agtatcggct ttcgatactt ggtaataaac accaaatgta cgtgcaaaac gtaggccgca
     2161 tggcgtgatt tattaatttt agtgaattgc attctgtcac tcaatcgggc attaatagat
     2221 atggcaataa tgctgcacac tgagcgctaa atgcaaattc tgaaagccta caaattccga
     2281 ctcgaaccga ccgaacaaca agcgcaacgc ctgcgccagt tgggcggttg tgctcgttat
     2341 gtctggaatc acgggctggc agaaaccaaa cgcattctgg atgctggcga aaaactgcct
     2401 tctgcctttg agctgaacag gatgctcacc agttggaaga aagacccaga aatggccttt
     2461 ctggccgagg catataccga caacctccag cagaagctca aggatttgca tggcgcgtgg
     2521 atgcgctgct ttgataagaa actcgccgcc aaggcacccg tattcaagaa aaaaagcgac
     2581 aaccgcgacg ctatccggtt cgtcaacttc gacaagtatt gccgccttga aaagggtcgc
     2641 gtgaagctgc ctgccgggct ggcgtgggtc aagttccgtc agtcccagcc agttgaaggg
     2701 aaaattaaaa acgcgaccgt cagccaagca gccgggcact ggtatatctc ctttcaggtc
     2761 gagcaggaga cgcttgagcc aattcacccc tccggctcaa tggtcgggct agatgcgggg
     2821 atcgccaagc tcgcaaccct gtcagacggc acggtgtttg agcccgtcaa cagcttcaaa
     2881 accaatcaga ccaagctggc aaggctccaa cgccagttga gcaaaaaggt aaagttcagc
     2941 agcaactgga agaaacaaaa agccaagatc cagcggctgc actcacatat cgccaatacc
     3001 cgccgcgatt accttcacaa ggtcacaacg actatcagca aaaaccacgc gatgatcgtc
     3061 attgaggatt tgaaggttgc cagcatgtca aaatcagcag cgggcacgac cagccagccg
     3121 gggcgcaacg tccgggcaaa atcagggctg aatcgttcga tattagatca gggctggttt
     3181 gagctgcgcc gccagctcga atataagcag gcatggcgag gcggtcaggt gttggcgata
     3241 aacccggcct acaccagcca gcagtgcgcc tgttgtgggc atactgcgaa ggataaccgc
     3301 cagacgcaaa gccagtttaa atgcgctgcg tgtggttatg aggcgaacgc cgatgtgaac
     3361 ggcgctcgca atattttagc ggcgggacac gccgttttag cctgtggagg cgtggtgcaa
     3421 tcaggccgcc cgatgaagca ggaacccggt actgcgcgag cagctatcac ccagtaatgg
     3481 gaatccccct cctgtgacgc gaagcgctaa ggtgggggag gatgtcaaag agtttgacgt
     3541 ttaggcgtca gataatgcag aaagcggccc tagtctgacg cgatgccagt gagccgactg
     3601 acgccaagtt agggtataga tcaggttgac gccccaactc tgggtttcgg ctcccggcca
     3661 ggagcggccg accgtcaaac gggtaagcag tcgctcaacg cctgagttaa gccgcggtgc
     3721 gcagtggcaa gcggcgtgcg aagcttagcg tagcacgcaa gcgccgccac gaagcaacgt
     3781 cggcttgaac gaattgttag acgggcgtca actatttgtc cgtgctcagg atgagttgtg
     3841 taataacttg accgacagag gcaactaatt cgtcacgttc tacggccgat agtttcgggg
     3901 ccgttgtata caccgctaca gcgtaatctc tctcctgggc tttaaaaaaa ccaatgtcgt
     3961 tccggccccc gttggcgcag gtaccagttt tctctccaac aacccaatct ttaggaaaac
     4021 ccgctcgtag tgtcgcgtct cccgtttggt ttccgatcag ccacctctca atggtgtggg
     4081 tcgaggtgga cgtcagtgcg ccgccataga ggactttagc cacagtacgt gccatagcaa
     4141 taggcgtagt tgtatctctg aggtcgccag gtgtgttgtc gctcatctcc ggctctttcc
     4201 ggtctagccg actcacagag tcgccaattt tacgaaaata ctgcgtcatt gcagcaggtc
     4261 cgccaatttc tctcagtaag aggttagtag ccccattgtc gctaagctgc accgcagctt
     4321 gcgctgcctc gagaaccgtc atgtgtcccg atgctagaaa ccgctccgtg gcaggagacc
     4381 atttgacgat catgtccggc ccatatgaaa gttttcgatc cccccgctcg gtgcctgagt
     4441 caattctttc aaagaccagc gcggctagcg gaaacttgaa cgttgagcac attgcaaaac
     4501 gctgcgccat tcggtggccc gcgacgatct ctccttgggg atcgacgatc gcaacaccga
     4561 tctgagctgc tttttcgcgc tctagcttct caagatcggt cttgaaggtt aatttttccg
     4621 acgcatatgc agagtgagcg atccctgcca gtaatagtgc gtgaatgaag cgcatggtga
     4681 tcccttgaga tggaaattat ctttgtacgc ccgtctaact tatggtttat ctgaccaaac
     4741 accggatacc cacaaccgtg gtcgttaaac aagctacctg cattacagcc tacagaccga
     4801 gaaagcttat gtctactggg ccaaggcatt tgtgttgtgg acggcccgca gccatggtgg
     4861 gtttcgacat ccgcgcgaaa tggggcaagc tgaagtcgag ggttttctga ccatgctcgc
     4921 caccgagaag caagtggcgc cggccaccca ccggcaggcg ctcaacgcgc tgttgttctt
     4981 gtatcggcag gtgctgggca tggaattgcc gtggatgcag cagattggtc ggccgccaga
     5041 acgcaagcgg attccggtgg tgctgacggt gcaggaggtt cagacgttgc tttcgcacat
     5101 ggcgggcacc gaagcgctgt tggccgccct gctttacggc agtgggttgc gcctgcgcga
     5161 agcgctgggc ctgcgggtca aggatgtgga tttcgaccgc cacgcgatca ttgtgcgcag
     5221 cggcaagggc gacaaggacc gcgtggtgat gctgcccagg gcgctcgtac ctcggttgcg
     5281 ggcgcagctg attcaggtcc gcgctgtgtg ggggcaggac cgtgccacgg ggcgcggagg
     5341 cgtgtatctg cctcatgcac tggagcgcaa gtaccccagg gcgggcgaga gctgggcctg
     5401 gttctgggtg tttccatcgg ccaagctgtc tgtggaccca caaaccggcg ttgagcgccg
     5461 ccaccacttg tttgaggaaa gactgaaccg gcaactaaaa aaagcggtag ttcaggctgg
     5521 cattgccaaa cacgtatctg tccacaccct gcgccactca ttcgccaccc acttgctgca
     5581 agcaggcaca gacatccgaa cgatgcaaga gttgttgggg cattcggacg tgagcacgac
     5641 gatgatctac acgcatgtgc tgaaagtcgc tgccggaggc acctccagcc cgctggacgc
     5701 cttggccttg cacttgtcgc ccggctgaat gacggttgtg gtgctttcat ctggatggcg
     5761 gctcagggtc gatagcgccc tcctgcatgt catgcctcgc aggtgcagac ttgcactgct
     5821 gctaggtaaa agccgtcgcg cgccttagcg tgctattttt cccgttttct gagacgaccc
     5881 caaacggccc gccaccccca cggtaactct ccccaacccc cacggtaact ctccccaacc
     5941 cccacggtaa ctctccccgc cccgatctga aaaaacccag acggggcgcg gctttccggg
     6001 cgattcgcaa aatcgatcca agattctcta agaatctcca ctaggcgcag cacagcccgc
     6061 gccccttcgg cgcggtctgg cattgcctca aatgcccggc caaggccgga aggctgaaca
     6121 cctctccccc cgccttcggc gaccccctcc gggggtaatg ggccagcggg gagagggggg
     6181 tggaggccgg tttcccggcc taaacccctc aggaggcgct ttctggcgtt tctggcgcat
     6241 ccccaaaggg taacccagcc tgacccggca tcggcgcgtc tgcggctttc tggggcgctt
     6301 tgcggggctt cgggtagccc gccttgcgca tggccttggt cagcgcctcg attgccgccc
     6361 aggtatccgc cgcccatgcc tcggacggct caaagtcccc ggccttgatc gtgtcggcaa
     6421 cctcaaaaat acgactggcg gcagacttgg cggaatactg tcggattcgc ttttcttctg
     6481 cctgtctttc tgtttcgatg tatgactgta gctctattct ttcttcgtct gtcagattct
     6541 caactaggcc gtctgacagc tcgtaagaat atacgtcaat agaccccatc atcttgacga
     6601 tggcgcggcg tttttccttg tcataccccc ggtaggcgag cacctgaacc cggcgacctt
     6661 ggcgcttaaa ctgcatggct gtttctccgc ttgactgtgt gattacattc tagtccgaca
     6721 gtcacacact gtcaaatgtt tattgtcggc ccttatttta atccgacagt cgcactcaaa
     6781 tgggttttta ctgtcaggtt aggttccgcc cgacagtctg ccgttctttc gctaccatag
     6841 cggcatggta tcagttcgcc caagtgcttt ggccaccctc tccccgctgg cccattaccg
     6901 cccttgggcg gtcgggctga gagcgcagcg agcgggccgg gtgggtggct gttcggtgct
     6961 tgcatggaga tgtgctatta tggtattgag gctgtggttt ggcatggagc aaggtgtaac
     7021 ctgcgcttcg cttggttctc accctccggc cttcggccta cgcccccact cccttcggtc
     7081 ggggggttgc tgcgcgtttc gcttcgcttc acttactgga gggcttcgcc catgaacact
     7141 gacgaatcca agcgccgccg ccgtgggcct gcgccgctcg acgccacgga caagcgcggg
     7201 cataccgttt ctgtgcgcct caacgaggcc gagcttgcgc ggctggattc gcagcgtgac
     7261 gcggtgcaga tgcagcgggg cgagtaccta cgcgccgccg ctctgcatcg cctgccgccc
     7321 accatcccgg cagcgaaccg ggaacagtgg gcagagttgg cccgcacagc ggccaacctg
     7381 aaccagatcg cccggcacct gaacgaaggg cagcggggcg acggggagcg catcggcaag
     7441 gcgctgggct tgcagttgag cgccgagctt gccaactgcc tgcgattgct ggtgctggtg
     7501 cgcggcgact tgataggcgt caagcacgac tcggaggacg tggaagatga aagcgaaggt
     7561 tagccgtggt ggcggttttc gcggtgcgct gaactacgtt tttgacgttg gcaaggaagc
     7621 cacgcacacg aaaaacgcgg aacgtgtcgg cggcaacatg gccgggaatg ccccccgcga
     7681 actgtcgcgg gagttctcag ccgtgcgcca gttgcgcccg gaaatcggca agcccgtctg
     7741 gcattgctcg ctgtcactgc ctcccggtga gcgcctgagc gccgagaagt gggaagccgt
     7801 gacggctgac ttcatgcagc gcatgggctt tgaccagacc aatacgccgt gggtggcagt
     7861 gcgccaccag gacacggaca aggatcacat ccacatcgtg gcaagccggg tagggctgga
     7921 cgggaaagtt tggctgggcc agtgggaagc ccgccgcgcc atcgaggcga cccaagagct
     7981 tgagcatacc cacggcctga ccctgacgcc ggggctgggc gatgcgcggg ccgagcgccg
     8041 gaagctgacc gacaaggaga tcaacatggc cgtgagaacg ggcgacgaac cgccgcgcca
     8101 gcgtctgcaa cggctgctgg atgaggcagt gaaggacaag ccgaccgcgc cagaactggc
     8161 cgaccggcta tatgccgcag gcgtaggcgt ccgggcaaac ctcgccagca ccgggcgcat
     8221 gaacggcttt tccttcgagg tggccggagt gccgttcaag ggcagcgact tgggcaaggg
     8281 ctacacatgg gcggggctac agaaatcagg ggtgacttat gacgaagcta gagaccgtgc
     8341 gggccttgaa cgattcaggc ccacagttgc agatcgtgga gagcgtcagg acgttgcagc
     8401 agtccctgag cctgatgcac gaggacttga agcgcctacc ggtcgcagtc tcgaccgaga
     8461 cggcgcagac cttggaaccg ctggcccgac tccagcagga cgtgacgcag gttctggaag
     8521 cctacgacaa ggtgacggcc attcagcgca agacgctgga cgagctgacg gagcagatga
     8581 gcgtgagcgc ggcgaaggcg ttcgagcgga aggccgggga gctggacaag accatctccg
     8641 acctgtccaa cagcctgtca gggctggaaa cgagcatcag cagcatgaag cagaccgcgg
     8701 agcaggtggt ggccttgccg ggcaatctgg tgagcgcaca gcagaatatg acggaagccg
     8761 ccaacaaact gaccgaggca gcgaacgaga cgcgcccgcg cctttggcgg caggcgctgg
     8821 ggctgattct ggccggggcc gtgggcgcga tgctggtagc gactgggcaa gtcgctttaa
     8881 acaggctagt gccgccaagc gacgtgcagc agacggcgga ttgggccaac gcgatttgga
     8941 acaaggccac gcccaaggag cgcgagttgc tggaacagat cgtcaatcgg cccgcgaact
     9001 agatccgacc gcctaccttg aggcgagcgg ctacaccgtg aagcgcgaag ggcgacacct
     9061 gtccgtcaag gctggcggtg atgaggcgta ccgcgtgacc cggcagcagg acgggcgctg
     9121 gctctggtgc gaccgctacg gcaacgacgg cggggacaat atcgaccttg tgcgcgagat
     9181 cgaacccggc accggctacg ccgaggccgt ctatcggctt tcaggtgcgc cgacagtccg
     9241 gcagcaaccg cgcccgagcg agccgaagcg ccaaccgccg cagctaccgg cgcaagggct
     9301 tgcagcccgc gagcatggcc gcgactacct caagggccgg ggcatcagcc cggacaccat
     9361 cgagcacgcc gagaaggcgg gcatggtgcg ctatgcagac ggtggcgtgc tgttcgtcgg
     9421 ctacgaccat acaggcaccg cgcagaacgc cacacgccgc gccattgccc ccgctgatcc
     9481 ggtgcagaag cgcgacctac gcggcagcga caagatctat ccgccgatcc tgccgggcga
     9541 cccggcaaag gtctggatcg tggaaggtgg cccggatgcg ctagccctgc acgacatcgc
     9601 caagcgcagc ggccagcagc cgcccaccgt catcgtgtca ggcggcgcga acgtgcgcag
     9661 cttcttggag cgtgacgacg tgcaagcgat cctgaagcgg gccgagcgcg tcaccgtggc
     9721 cggggaaaac gaaaagaacc ccgaggcgca ggccaaggcc gacgccgggc accagaaaca
     9781 ggcgcagcgg gtggccgaaa tcaccgggcg cgaggtgcgc caatggacgc ccaagcctga
     9841 gcagggcaag gacttggccg acataaacgc ccgtcaggtg gccgagatcg agcgcaagcg
     9901 acaggcagag atcgaggccg agagggcacg aaaccgcgag ctttcacgga agagccgggg
     9961 gtatgatggc cccagcttcg gcagataaga gcgcctgtca gtcggtgtga tgaaccgacc
    10021 gacaggctaa ccatcatcaa cctacgcagg aggccgat
//
