LOCUS       NZ_OW967268             3576 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Citrobacter freundii isolate 22 plasmid P5, complete sequence.
ACCESSION   NZ_OW967268
VERSION     NZ_OW967268.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065677
            Assembly: GCF_905329885.2
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905329885.2-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:17:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,331
            CDSs (total)                      :: 5,210
            Genes (coding)                    :: 5,079
            CDSs (with protein)               :: 5,079
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 32 of 131
            Pseudo Genes (incomplete)         :: 97 of 131
            Pseudo Genes (internal stop)      :: 22 of 131
            Pseudo Genes (multiple problems)  :: 19 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967268.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /isolate="22"
                     /isolation_source="Hospital Universitario Gregorio Maranon
                     (Madrid)"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="P5"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            join(3445..3576,1..57)
                     /locus_tag="LQ176_RS26600"
     CDS             join(3445..3576,1..57)
                     /locus_tag="LQ176_RS26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044703047.1"
                     /translation="MTKQQKTALNMAKFIQAQSLFLLGNLNEQDLDTEAGLCEKLHEDA
                     ELLFRTLDIRLDDFQED"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            60..491
                     /locus_tag="LQ176_RS26605"
                     /old_locus_tag="AI2826V1_5177"
     CDS             60..491
                     /locus_tag="LQ176_RS26605"
                     /old_locus_tag="AI2826V1_5177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044703046.1"
                     /translation="MARVSISEAARLVSVSRPTIYKLLKSGELSYTSVVKHGKPVKTID
                     TAELIRLFGALDVVSGDKSDTVKFDGVTTSVNSDFLHSLQHQVALLQAENTGLKDAVNA
                     RDEHISSLRQAMQLLEHKQSTSDPQNVPAKSWWQFWKSH"
                     /name="helix-turn-helix domain-containing protein"
     gene            1648..2586
                     /locus_tag="LQ176_RS26610"
                     /old_locus_tag="AI2826V1_5179"
     CDS             1648..2586
                     /locus_tag="LQ176_RS26610"
                     /old_locus_tag="AI2826V1_5179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013185833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200002678.1"
                     /translation="MSVLDFLSSGGMASIGGDLSEEEQKHTNEIVKLFAKKLREYARTL
                     QIPASNIKINKCSFLFVAKDDFSYHFVFADKPGGGNNLVYRNLSEYRQLELQSIVHQVR
                     IDVGDVVWAFSCPVSLSGEELDTYINKLSIEYIQSEIQSSKDNNDGRISEEAMNVPEIA
                     SGLEKFRKDYPKGKKTAFIIMQFGSTAAHVKLVDAIKKELSKHNIIALRADDKEYMDDL
                     FSNIKTYMYGCDFGIAIFERITEDNFNPNVSLEVGYMMGMGKQVLLLKDKTLSSLHTDL
                     AGKLYKPFDVFDIESSLSTHIGKWLSDKGFL"
                     /name="hypothetical protein"
     gene            complement(2896..3027)
                     /locus_tag="LQ176_RS26615"
     CDS             complement(2896..3027)
                     /locus_tag="LQ176_RS26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072052181.1"
                     /translation="MHEPPVQSNRCALSGNYRLESNPKRHDKTPLAAATGNGMYKIN"
                     /name="replication initiation protein"
     Typing          complement(2920..3264)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.509"
                     /coverage="80.0"
                     /evalue="2.99e-149"
ORIGIN
        1 gcggaactgc tttttcgtac tctggatatt cgtctggatg attttcaaga ggattagtaa
       61 tggctcgcgt atccattagt gaagctgcac gtcttgtcag tgtcagtcgc ccgactattt
      121 acaagctgct taaatctggt gagcttagct acacctccgt tgtaaagcat ggaaaacccg
      181 ttaaaaccat tgatacagct gagcttattc gtctttttgg tgctcttgat gttgtcagtg
      241 gtgataaaag tgacactgta aaatttgacg gtgtcactac gtcggttaac agtgattttt
      301 tacattcttt acagcatcaa gtggcgcttt tacaggcgga gaataccggg cttaaagatg
      361 cagtgaatgc acgggatgag catataagtt cacttcgtca ggccatgcaa cttctggagc
      421 ataaacagag tacttctgac ccgcagaatg ttcctgcaaa gtcctggtgg cagttctgga
      481 aatcccattg agtcattatc aatcatgcat tccggtcata gcgttcggcc agccaccagg
      541 ctatcaccgg atatggatgt ttatactttc tcatctgacc gctttacccg catgtttgat
      601 ttctgatggc tgataaaggc caaaaaatcg tgtcacaccc cacgcagtat acacggctaa
      661 tgccggaagc ctttgctgac aaggcttccg ggggtatacc accccttgta tgaaatggta
      721 tacccgcatc tgtttttatg cggttaaagc cagttctggc aagacctgta gggggtatac
      781 caggtggtaa ctggcttggt atacctgaaa agctgctctg agggcttcag tgccacacag
      841 gataagggat tgcacttggt ataccctttt tctgtttgtg tggtgcgata tcagtatctc
      901 tccctgcgcg gtgttataga taaaagacct gtcatggatg cgccccgaca ccctgccagc
      961 gcctcacgac cccgcaataa attgcgtgcc cgttcaccgc tttctttagc cagtctgtgc
     1021 cagactgtta tctgaatggc ttactagtgt ggaggtaaaa aggtgaaaga atggtttgcg
     1081 acccttatcg atgacctgaa atttctgggg gcggagttta agagagagtt ttcggatctg
     1141 gagatcaggc cgatgacggc tcaggagaac cgacggagac atgatgagat gcaccgttct
     1201 tccgtggccg ggaagttagc acggggcgct ttgcaaatca gggacgggaa actgaccccc
     1261 tatggtatgg gacggaacaa ataggcttca tcaatcccgg attcccgggc tagtaaaccg
     1321 ggaaaaatct cccttttagc ggctgttttc cgtcaaatca cgcaccatgc agccgggagc
     1381 atagttattc accctctcag gattcatctg tggcgttctg agcgttttcc cccgggtaaa
     1441 ctgtacaaaa cgacgcactc accccaaaag cgctcagagt gcgtctgagc gatgcgcgta
     1501 atgagtgctt atgttaaata gatggcagta gactcaaggt gggttgtatc attatcaagt
     1561 gaataggtga tgttaccatt aagtagtggt taggttttgg gatgtccggg ccaagcgttc
     1621 tttagaattg gaaaaaggaa attaactatg tctgttttgg attttttatc ttccggtggg
     1681 atggctagta ttggtggaga tctgtctgaa gaagaacaaa aacatactaa tgaaatagtt
     1741 aaattatttg ccaaaaaatt gagagagtat gctaggacat tacaaattcc agcatccaat
     1801 atcaaaataa ataaatgttc atttttgttt gtcgcaaaag acgatttttc ttatcatttc
     1861 gtgtttgctg ataaacctgg tggcggtaat aatttagtat atagaaactt atctgaatat
     1921 agacagcttg aacttcaaag tatcgtccat caggttcgga ttgatgttgg tgatgttgtt
     1981 tgggcgttct cttgcccagt ctctctctcc ggggaagaac ttgatacata tattaataaa
     2041 ctctctattg aatatattca gtctgaaatt caaagtagta aagataacaa tgatggtaga
     2101 atttcagaag aggctatgaa tgtccctgaa atagcttctg gccttgaaaa attcagaaaa
     2161 gactatccaa aagggaaaaa aacagcattt atcatcatgc agtttggtag cacggcagcg
     2221 cacgtaaagc ttgttgatgc aataaagaaa gagttgtcta aacacaacat aatagcttta
     2281 agggctgatg ataaagagta tatggatgat ctcttttcaa atatcaaaac atatatgtat
     2341 gggtgcgatt tcggtattgc tatttttgag aggattacag aagataattt taaccctaat
     2401 gtttcattgg aagttggata tatgatggga atggggaagc aggttttact tttaaaggat
     2461 aagactctaa gttcattaca tacagactta gcaggtaaac tttataagcc atttgatgtt
     2521 ttcgatattg agtcatccct cagcactcat attgggaaat ggctatcaga caagggattt
     2581 ttatagtttc cggaacaagt accgtatgtg cggtacttgt ttgacatcgt tagttatcga
     2641 atggggttat gcgaaaatca tggtcaaatt tgagacttga cgcgtttgat tgatgagatc
     2701 ttcttgagat ccttcgtctc tcgtcgtagt ctcttgttcc agaaacgaaa aaaccgactg
     2761 gggagtcggt tttttcgagg gttcagcaag ttggggaact tctgaaccgt ggtaacaggg
     2821 tgtacaagac cgctgaaacc gtaatctgtc ctttcagttt agccgtagtt tagctcggac
     2881 ttcaagaact ctcattcagt taatcttgta catcccatta ccagtggccg ctgccagtgg
     2941 cgttttgtcg tgtcttttcg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3001 gttggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga
     3061 actgagtgtc aggcgtggaa tgagagaacc gcggccataa cagcggaatg acaccggtaa
     3121 accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc
     3181 tttaaagtcc tgtcgggttt cgccaccact gatttgagcg tcaaattttg tgatgcttgt
     3241 caggggggcg aagcctatgg aaaaacgccc tggtcgggca tggctttatc gttcttgttc
     3301 tggcttttcg ttacgcttac cgtctacgtt atttgtctct taggaagatg cgtccgctcg
     3361 ccgcagataa gtgaccgagc gtagcgagcg aacggtcgag gaagcggaaa agaaccggat
     3421 atcatttgga gcactgacac tgtgatgacg aaacaacaga aaacggcact gaatatggcg
     3481 aaattcattc aggcgcaatc cctgttcttg ctgggtaatc tcaatgaaca ggatctcgat
     3541 actgaagccg gcttgtgcga aaaattgcat gaggat
//
