LOCUS       NZ_OW967010             2313 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli isolate 624 plasmid P7, complete sequence.
ACCESSION   NZ_OW967010
VERSION     NZ_OW967010.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA8065689
            Assembly: GCF_905330065.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Alioto,T., Alioto,T. and Gomez Garrido,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2022) CNAG-CRG, CNAG, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_905330065.2-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 11:21:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,485
            CDSs (total)                      :: 5,369
            Genes (coding)                    :: 5,137
            CDSs (with protein)               :: 5,137
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 91 of 232
            Pseudo Genes (incomplete)         :: 162 of 232
            Pseudo Genes (internal stop)      :: 40 of 232
            Pseudo Genes (multiple problems)  :: 51 of 232
            Pseudo Genes (short protein)      :: 1 of 232
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            OW967010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="624"
                     /isolation_source="Hospital General de Alicante (Alicante)"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="P7"
                     /geo_loc_name="Spain"
                     /collection_date="2018"
     gene            complement(join(1910..2313,1..94))
                     /locus_tag="LQ179_RS27265"
     CDS             complement(join(1910..2313,1..94))
                     /locus_tag="LQ179_RS27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_023156898.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNVTQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
     gene            1451..1876
                     /gene="mobA"
                     /locus_tag="LQ179_RS27270"
                     /old_locus_tag="AI2834V1_5302"
     CDS             1451..1876
                     /gene="mobA"
                     /locus_tag="LQ179_RS27270"
                     /old_locus_tag="AI2834V1_5302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_024257935.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRLASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLYELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDIYNEMDLIKDEIKIIKRLHKNKYPGSDLFK"
                     /name="mobA"
     Typing          446..704
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.466"
                     /coverage="100.0"
                     /evalue="6.900000000000001e-74"
ORIGIN
        1 cttgtgttac gtttccagtt tctctatcta taacctcagt aatattttgt cttaaaactt
       61 ttttcttgct atccgttctt tcagtaatgt tcattttatc gcctttgtca ctttataaag
      121 gtgacaaaga atataatacc caaaaaacaa aatgtcactc aatatagatt acgcgaatac
      181 cgcccacacg ttacaggtgt atcctgtaca cgttacaggt gtatcttcta cacgttacaa
      241 cattggcgat cagcccgtat gtagcctaat tcggatatct tatattttta aatgtatcca
      301 caagcacgag gcataatctc tatttacgaa gtgacaattt taatattgag cgtgcgtgct
      361 caatgtagct tccggttctc ttccgcttcc tcgcccactc gcgagcctgg cggctcggtc
      421 gctggggctg cggcgagcgg tgccacctct cgcatatcgt tgatgatccc gatttgatct
      481 caggcgatcg agtcgctggt catctgttaa acgcgctcag agcctcccag acgcacgaaa
      541 cggggtctaa atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agggtttcag
      601 gaggccaagc gcgccgatac ccctaaccag gggctttcag gtcgccagga accgcgccag
      661 ccccctgcaa agcccatttg cacgggatgg gcagctactc ggttggtggg tggtcataga
      721 ccgtcgatgt tgcagcggtg ggggtctggg gttctcccca gacggtttcc ggttgcgctc
      781 aaggcgctgc cggaaccgtg gcgactcggt aggtttgaag cgcgtttccg gcgttcgttg
      841 gcacgcaaac catccccatg gttagttctt gccggaaacg cgccctgatg ttctatcgta
      901 tcgatggtta tatgtcgagg ttgtcagcag ctggcggagc actgcatggg gattaatgct
      961 cccattgcca ggatgaaata aagattagga gcgacgttaa gttatatttt taatatataa
     1021 acgcgatatc aataactcac atagttatat tttatgcata tgctaccatc ttgctctttt
     1081 gcctcctgct ctctggacga atcccgcatt accaacaggc aatggattat ataaccaaga
     1141 agatctaatc tttattcctc taataatcca caaacgacag acaacaaaga catcaattaa
     1201 ttcggtgtta tttattaaat aacaagcaat aatttttaaa atctgtgtaa ataatattga
     1261 atgaagcatc atttttttgt atcagaaata ccaggttacg acgcggtatt ttatttttgc
     1321 cgaaaagttg cagcggaaaa tgggacagga tgtgtattcg tcataccgcg aatatattcg
     1381 cttgcatgag aatacctcct gtcagggcaa gccccgacac cccaaacgtg caaaatggag
     1441 agtcattgcg atggctaaaa ataaacgtag tggttcagaa gtacggcaga gaactaaggt
     1501 tatcaccctt agagtaaata acagactcgc aagcgaaatc agacggcgag cgaagaacga
     1561 aggactgaca atttcagaat atatcaggac ggcaagttta aataatgaaa taaaacagcg
     1621 cgtaccttcc aggtatttat atgaattaat ccgacttggt cggatgcaga agaaattatt
     1681 tgataaggga aagcggccga aggataaaga gtacctcgag gtgatgcata aaataatttt
     1741 actttgcgat gaaatgaaaa tagtaacgaa gcgaatttct gatatatata atgaaatgga
     1801 tttaataaag gatgaaataa aaataataaa aaggctacat aaaaataaat atccaggttc
     1861 ggatttattc aaataaggtc tttttaaaaa gaccttattt aggtgcgcat tattttattt
     1921 cttccgttgt tataatagtt tcaccgttag gtgaatattt aattctcatt tcaaactcgc
     1981 gacgctgttc aattattttt ttccattcac cacgagcaaa taaattaggg ttcgcctgat
     2041 attcattacc gccacaacta gcaataatcc ccatttttga taatgaatac aatctgttac
     2101 gtaatgttcc gtcttttatt cccaataatt tacagatctc cttacggtat ctagttgata
     2161 atgcaatata tccgtcataa tccaatttcc taagaattaa aaataaaaca tcttttagag
     2221 aatcaggtgc ttttgtgaca gcacataaat tatcaatata aattttcaca tacgcaggtt
     2281 cttgtggaaa ttgaactgtt atatcggtta ttt
//
